FastQCFastQC Report
Wed 25 May 2016
SRR1294510_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294510_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244087
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC520952.3214340620483966No Hit
ACCATGTACTCTGCGTTGATACCAC317831.4162998136881502No Hit
GTACATGGTAAGCAGTGGTATCAAC315631.4064962722033505No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA275811.2290521713284734No Hit
GTGGTAAGCAGTGGTATCAACGCAG261391.164794413050831No Hit
CATGTACTCTGCGTTGATACCACTG254381.1335567649560823No Hit
GGTAAGCAGTGGTATCAACGCAGAG223410.9955496377814229No Hit
GTATCAACGCAGAGTACATGGGAAG188270.8389603433378474No Hit
ACTCTGCGTTGATACCACTGCTTAC184590.8225616921269095No Hit
CCATGTACTCTGCGTTGATACCACT162520.7242143464134858No Hit
GGTATCAACGCAGAGTACATGGGAA154890.6902138820821118No Hit
GTACTCTGCGTTGATACCACTGCTT151860.6767117317644102No Hit
GTACATGGGAAGCAGTGGTAAGCAG130200.5801914096913355No Hit
GCAGTGGTATCAACGCAGAGTACAT129630.5776514012157283No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA128590.5730169997865502No Hit
TATCAACGCAGAGTACATGGGAAGC119280.5315301946849654No Hit
GTGGTATCAACGCAGAGTACATGGG118630.5286336937917291No Hit
GTACATGGGAAGCAGTGGTATCAAC117930.5255143851374746No Hit
GAGTACATGGTAAGCAGTGGTATCA116480.5190529600679474No Hit
GCTTACCACTGCTTCCCATGTACTC108950.48549811125860987No Hit
GCTTACCATGTACTCTGCGTTGATA108070.4815766946646899No Hit
GCGTTGATACCACTGCTTACCATGT104940.467628928824952No Hit
AAGCAGTGGTATCAACGCAGAGTAC104160.46415312775306844No Hit
GCGTTGATACCACTGCTTACCACTG102910.458582933727614No Hit
GTACATAAGCAGTGGTATCAACGCA91780.4089859261249675No Hit
ATGGTAAGCAGTGGTATCAACGCAG90500.40328204744290214No Hit
CTGCTTACCATGTACTCTGCGTTGA89110.39708799168659686No Hit
GAGTAAGCAGTGGTATCAACGCAGA87150.3883539274546842No Hit
GTATCAACGCAGAGTACATGGTAAG81570.36348858132505557No Hit
CAGTGGTATCAACGCAGAGTACATG77600.34579764510021227No Hit
GTGATAAGCAGTGGTATCAACGCAG76140.3392916584784815No Hit
ACGCAGAGTACATGGGAAGCAGTGG73850.3290870630238489No Hit
TATCAACGCAGAGTACATGGTAAGC70490.31411438148342735No Hit
ACGCAGAGTACATGGTAAGCAGTGG68370.3046673324162566No Hit
ATTCCATTCCATTCCATTCCATTCC64350.2867535884303951No Hit
GTACATGATAAGCAGTGGTATCAAC61560.2743209153655808No Hit
AAAAAGTACTCTGCGTTGATACCAC58850.2622447347183955No Hit
GGTATCAACGCAGAGTACATGGTAA57380.2556941865444611No Hit
GCTTATGTACTCTGCGTTGATACCA55630.24789591490882484No Hit
GAATGGAATGGAATGGAATGGAATG53890.24014220482539225No Hit
GAGTACATGGGAAGCAGTGGTAAGC53430.23809237342402498No Hit
TACCACTGCTTACCATGTACTCTGC52800.23528499563519595No Hit
ATCATGTACTCTGCGTTGATACCAC52650.23461657235214142No Hit
GAGTACATGGGAAGCAGTGGTATCA52050.2319428792199233No Hit
GTATCAACGCAGAGTACTTTTTTTT51770.2306951557582215No Hit
GCTTACTCTGCGTTGATACCACTGC50470.22490215397174884No Hit
GTAAGCAGTGGTATCAACGCAGAGT49570.2208916142734217No Hit
AAAGTACTCTGCGTTGATACCACTG48070.21420738144287632No Hit
GTGGTATCAACGCAGAGTACATGGT47340.2109543881320109No Hit
GCTTCCCATGTACTCTGCGTTGATA44860.1999031231855093No Hit
CTTCCCATGTACTCTGCGTTGATAC41360.18430657991423685No Hit
TGGTAAGCAGTGGTATCAACGCAGA38520.17165109908840434No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT38440.17129460667077526No Hit
GCGTTGATACCACTGCTTACTCTGC38360.17093811425314615No Hit
GCAGAGTACATGGTAAGCAGTGGTA38310.170715306492128No Hit
GATAAGCAGTGGTATCAACGCAGAG38290.1706261833877207No Hit
TATCAACGCAGAGTACTTTTTTTTT38150.1700023216568698No Hit
ACTCTGCGTTGATACCACTGCTTAT37670.16786336715109532No Hit
GCGTTGATACCACTGCTTCCCATGT37380.16657108213718985No Hit
CTGCTTACCACTGCTTCCCATGTAC36470.16251598088665903No Hit
GTACATGGTAAGCAGTGGTAAGCAG34980.15587630960831733No Hit
GGTATCAACGCAGAGTACTTTTTTT34310.15289068561067373No Hit
CTTATGTACTCTGCGTTGATACCAC32890.1465629451977575No Hit
CTGCTTCCCATGTACTCTGCGTTGA31810.14175029755976484No Hit
GTTGATACCACTGCTTACCATGTAC30340.1351997493858304No Hit
CTGCTTACTCTGCGTTGATACCACT30100.13413027213294315No Hit
GATACCACTGCTTACCATGTACTCT28730.12802533948104508No Hit
GCTTACCACTGCTTACCATGTACTC28110.12526252324441967No Hit
GTACATGGGAAGTAAGCAGTGGTAT28050.12499515393119785No Hit
GCGTTGATACCACTGCTTATCACTG26650.11875653662268887No Hit
ATCAACGCAGAGTACATGGGAAGCA26440.11782074402641252No Hit
TCCATGTACTCTGCGTTGATACCAC26400.11764249781759797No Hit
GAACAAAAAAAAAAAAAAAAAAAAA26210.1167958283257289No Hit
GTACATGGGAAGCAGTGATAAGCAG26110.11635021280369255No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25840.11514705089419439No Hit
GTTGATACCACTGCTTACCACTGCT25730.1146568738199544No Hit
ATACCACTGCTTACCATGTACTCTG25640.11425581985012166No Hit
ACTCTGCGTTGATACCACTGCTTCC25190.11225055000095809No Hit
CCACTGCTTACCATGTACTCTGCGT25150.11207230379214352No Hit
CCACTGCTTCCCATGTACTCTGCGT24680.10997791083857265No Hit
TACCACTGCTTACCACTGCTTCCCA24510.10922036445111084No Hit
GTATCAACGCAGAGTACATGGGAAA24000.10694772528872544No Hit
GTATCAACGCAGAGTAAGCAGTGGT23760.10587824803583819No Hit
GCTTATCACTGCTTCCCATGTACTC23740.1057891249314309No Hit
TACCACTGCTTCCCATGTACTCTGC23560.10498701699176546No Hit
GTACATGGCAAGCAGTGGTATCAAC23500.10471964767854366No Hit
GCAGTGGTAAGCAGTGGTATCAACG23160.10320455490362004No Hit
TTCCCATGTACTCTGCGTTGATACC23080.10284806248599097No Hit
TGATACCACTGCTTACCATGTACTC23010.10253613162056552No Hit
TATGTACTCTGCGTTGATACCACTG23000.10249157006836188No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT22960.10231332385954733No Hit
AAGCAGTGGTAAGCAGTGGTATCAA22760.10142209281547461No Hit
GAGTACATGATAAGCAGTGGTATCA22670.1010210388456419No Hit
GAGTACATAAGCAGTGGTATCAACG22650.10093191574123463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACACT704.4510307E-714.9248596
GAACAAA8650.014.8427491
GTTCTAA456.708349E-414.7938831
AACGTTG1101.2732926E-1114.6791512
CGGTACT400.005242048814.2655311
GAATTCG400.00528566114.24772615
GTACAAA3200.013.9683321
CAACGTT1101.8735591E-1013.81536111
TTCTCGT500.001501965613.29758313
TCTTATA604.0918656E-412.6671872
ATTATGC604.099566E-412.6643643
ACAACGT1155.34601E-912.38849910
CACAACG2400.012.268339
CACTCAA701.0936257E-412.2115210
TAACCTA701.0938532E-412.2112485
CTCGTTT550.003072844412.0889815
CTTCTCG550.003073357312.08871112
TGTATCG550.003074896512.0879025
CTGCTAA3900.011.9489061
AAAAGTA10900.011.7665372
Produced by FastQC (version 0.11.5)