FastQCFastQC Report
Wed 25 May 2016
SRR1294510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244087
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC549232.4474541316802783No Hit
ACCATGTACTCTGCGTTGATACCAC335621.495574815058418No Hit
GTACATGGTAAGCAGTGGTATCAAC332311.4808249412790147No Hit
GTGGTAAGCAGTGGTATCAACGCAG295501.316793867617432No Hit
CATGTACTCTGCGTTGATACCACTG271191.2084647342103938No Hit
GGTAAGCAGTGGTATCAACGCAGAG250971.1183612756546426No Hit
GTATCAACGCAGAGTACATGGGAAG197480.8800015329173958No Hit
ACTCTGCGTTGATACCACTGCTTAC192770.8590130418294835No Hit
CCATGTACTCTGCGTTGATACCACT179380.7993451234288154No Hit
GGTATCAACGCAGAGTACATGGGAA160460.7150346666595369No Hit
GTATCAACGCAGAGTACTTTTTTTT148970.6638334431775595No Hit
GTACTCTGCGTTGATACCACTGCTT148800.6630758967900977No Hit
GCAGTGGTATCAACGCAGAGTACAT139910.6234606768810657No Hit
GTACATGGGAAGCAGTGGTAAGCAG139610.6221238303149565No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA134430.5990409462734734No Hit
TATCAACGCAGAGTACATGGGAAGC129840.5785871938120046No Hit
GAGTACATGGTAAGCAGTGGTATCA124810.556172733053576No Hit
GTGGTATCAACGCAGAGTACATGGG124620.5553260635617069No Hit
AAGCAGTGGTATCAACGCAGAGTAC124310.5539446554433941No Hit
GTACATGGGAAGCAGTGGTATCAAC123260.5492656924620124No Hit
GTACTTTTTTTTTTTTTTTTTTTTT110110.4906672513142316No Hit
GCTTACCACTGCTTCCCATGTACTC109810.4893304047481225No Hit
GCTTACCATGTACTCTGCGTTGATA108130.4818440639779117No Hit
GCGTTGATACCACTGCTTACCATGT107430.47872475532365727No Hit
GCGTTGATACCACTGCTTACCACTG106270.4735556152680355No Hit
TATCAACGCAGAGTACTTTTTTTTT103210.459919780293723No Hit
ATGGTAAGCAGTGGTATCAACGCAG96760.43117757912237803No Hit
GTACATAAGCAGTGGTATCAACGCA93590.4170515670738255No Hit
GGTATCAACGCAGAGTACTTTTTTT92480.41210523477922206No Hit
CTGCTTACCATGTACTCTGCGTTGA91530.4078718873198766No Hit
GAGTAAGCAGTGGTATCAACGCAGA89520.3989150153269459No Hit
GTGATAAGCAGTGGTATCAACGCAG86160.3839423337865243No Hit
CAGTGGTATCAACGCAGAGTACATG85410.38060021737125166No Hit
GTATCAACGCAGAGTACATGGTAAG84700.3774363471647935No Hit
ACGCAGAGTACATGGGAAGCAGTGG79720.355244694167383No Hit
ACGCAGAGTACATGGTAAGCAGTGG74930.33389971066184154No Hit
TATCAACGCAGAGTACATGGTAAGC73600.327973024218758No Hit
GAGTACTTTTTTTTTTTTTTTTTTT65990.2940616829917913No Hit
ATTCCATTCCATTCCATTCCATTCC65820.2933041366043295No Hit
GGTATCAACGCAGAGTACATGGTAA60290.268661598235719No Hit
GTACATGATAAGCAGTGGTATCAAC60020.26745843632622085No Hit
GAGTACATGGGAAGCAGTGGTAAGC59540.26531948182044635No Hit
AAAAAGTACTCTGCGTTGATACCAC59100.2633587735234864No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58530.26081876504787915No Hit
ATCATGTACTCTGCGTTGATACCAC57160.25471383239598105No Hit
GCTTATGTACTCTGCGTTGATACCA56500.25177276995054115No Hit
GAATGGAATGGAATGGAATGGAATG56020.24963381544476662No Hit
GTAAGCAGTGGTATCAACGCAGAGT55610.2478067918044176No Hit
TACCACTGCTTACCATGTACTCTGC54220.2416127360481122No Hit
GAGTACATGGGAAGCAGTGGTATCA54100.2410779974216686No Hit
GCTTACTCTGCGTTGATACCACTGC52500.2339481490690869No Hit
AAAGTACTCTGCGTTGATACCACTG49500.22057968340799622No Hit
GTGGTATCAACGCAGAGTACATGGT48120.21443018920389448No Hit
GCTTCCCATGTACTCTGCGTTGATA45720.20373541667502193No Hit
GTGGTATCAACGCAGAGTACTTTTT45220.20150733906484017No Hit
GATAAGCAGTGGTATCAACGCAGAG44840.19981400008110203No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT43490.19379819053361122No Hit
CTTCCCATGTACTCTGCGTTGATAC43000.19161467447563307No Hit
ACTCTGCGTTGATACCACTGCTTAT41200.18359359507897866No Hit
TGGTAAGCAGTGGTATCAACGCAGA41200.18359359507897866No Hit
GCAGAGTACATGGTAAGCAGTGGTA40820.18190025609524052No Hit
GCGTTGATACCACTGCTTCCCATGT38940.17352268428095702No Hit
GCGTTGATACCACTGCTTACTCTGC38690.17240864547586615No Hit
GTACATGGTAAGCAGTGGTAAGCAG38350.17089355270094253No Hit
CTGCTTACCACTGCTTCCCATGTAC37760.16826442112092801No Hit
CTTATGTACTCTGCGTTGATACCAC35740.15926298757579363No Hit
CTGCTTCCCATGTACTCTGCGTTGA32670.14558259104927748No Hit
CTGCTTACTCTGCGTTGATACCACT31460.1401906432326376No Hit
GATACCACTGCTTACCATGTACTCT30810.13729414233940126No Hit
GTACATGGGAAGTAAGCAGTGGTAT30630.13649203439973584No Hit
GCAGTGGTATCAACGCAGAGTACTT30420.1355562418034595No Hit
GTTGATACCACTGCTTACCATGTAC30170.1344422029983686No Hit
GCTTACCACTGCTTACCATGTACTC29670.13221412538818683No Hit
ATCAACGCAGAGTACATGGGAAGCA29040.12940674759935777No Hit
ACTCTGCGTTGATACCACTGCTTCC28320.12619831584069602No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA28110.12526252324441967No Hit
ATACCACTGCTTACCATGTACTCTG27550.12276707632101608No Hit
GTACATGGGAAGCAGTGATAAGCAG27360.121920406829147No Hit
GCAGTGGTAAGCAGTGGTATCAACG27030.12044987560642703No Hit
TCCATGTACTCTGCGTTGATACCAC26810.11946952145794705No Hit
GTATCAACGCAGAGTACATGGGAAA26560.11835548265285616No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT26520.1181772364440416No Hit
GCGTTGATACCACTGCTTATCACTG26510.11813267489183797No Hit
GTACATGGCAAGCAGTGGTATCAAC26160.11657302056471072No Hit
GCTAAGCAGTGGTATCAACGCAGAG26030.11599372038606348No Hit
CCACTGCTTACCATGTACTCTGCGT25790.11492424313317622No Hit
CATGGTAAGCAGTGGTATCAACGCA25770.11483512002876893No Hit
CCACTGCTTCCCATGTACTCTGCGT25310.1127852886274017No Hit
GTTGATACCACTGCTTACCACTGCT25130.11198318068773626No Hit
TACCACTGCTTACCACTGCTTCCCA25050.11162668827010719No Hit
AAGCAGTGGTAAGCAGTGGTATCAA24740.11024528015179448No Hit
GCTTATCACTGCTTCCCATGTACTC24340.10846281806364903No Hit
GAGTACATGATAAGCAGTGGTATCA24000.10694772528872544No Hit
GCAGAGTACTTTTTTTTTTTTTTTT23860.10632386355787454No Hit
GTATCAACGCAGAGTAAGCAGTGGT23750.10583368648363456No Hit
GAGTACATAAGCAGTGGTATCAACG23690.10556631717041273No Hit
TACCACTGCTTCCCATGTACTCTGC23530.10485333233515455No Hit
TGATACCACTGCTTACCATGTACTC23460.10454140146972911No Hit
GCAGAGTACATGGGAAGCAGTGGTA23020.10258069317276915No Hit
TTCCCATGTACTCTGCGTTGATACC22820.10168946212869644No Hit
TATGTACTCTGCGTTGATACCACTG22750.10137753126327098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAA453.5194455E-516.900371
CGCTAAG602.5625339E-514.2539632
CACAGCG1102.752131E-912.9535289
GAACAAA3600.012.9393451
AGAATAG853.9342467E-612.2972635
GTCCAGG1103.7807695E-812.0991281
GCGTTGT550.00307303312.088881513
CAATACA802.8601935E-511.8780374
CTGCTAA4000.011.4077481
AAAAGTA10350.011.3848092
AAAAAGT10400.011.1518061
CCAATAC600.00586585611.0861693
CCTATTC600.00586585611.0861693
CTGTCGC600.00588047411.0824629
CGTCGAT600.005885353311.08122714
CCCAAAA2500.011.0274911
ACAGCGT951.3620145E-510.99913610
ATAGGAG700.001488669710.859923
GGGACTG700.001488669710.859925
TATTAAT803.762401E-410.6904732
Produced by FastQC (version 0.11.5)