FastQCFastQC Report
Wed 25 May 2016
SRR1294509_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294509_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023212
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC92320.4563041342182628No Hit
GTACATGGTAAGCAGTGGTATCAAC84010.4152308309756961No Hit
ACCATGTACTCTGCGTTGATACCAC81960.40509842764870907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA69680.3444028604021724No Hit
CATGTACTCTGCGTTGATACCACTG52500.2594883778862521No Hit
GTGGTAAGCAGTGGTATCAACGCAG49400.24416620700154013No Hit
GGTAAGCAGTGGTATCAACGCAGAG44890.22187491968216874No Hit
CCATGTACTCTGCGTTGATACCACT39760.19651919818585495No Hit
GTGGTATCAACGCAGAGTACATGGG39280.19414673301660923No Hit
GAGTACATGGTAAGCAGTGGTATCA32690.161574763297173No Hit
ACTCTGCGTTGATACCACTGCTTAC32000.15816434461638226No Hit
GCTTACCATGTACTCTGCGTTGATA30980.15312285613173507No Hit
GTATCAACGCAGAGTACATGGGAAG30560.15104694910864505No Hit
GTACTCTGCGTTGATACCACTGCTT27950.1381466697508714No Hit
GCAGTGGTATCAACGCAGAGTACAT27840.13760297981625255No Hit
GCGTTGATACCACTGCTTACCATGT26770.13231435954314227No Hit
GTACATAAGCAGTGGTATCAACGCA25160.12435671595463055No Hit
GTATCAACGCAGAGTACTTTTTTTT25040.12376359966231913No Hit
GGTATCAACGCAGAGTACATGGGAA24610.12163826628153648No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA24170.11946350654306125No Hit
GTACATGGGAAGCAGTGGTATCAAC21960.10854028149299233No Hit
CTGCTTACCATGTACTCTGCGTTGA21870.10809544427375875No Hit
GTATCAACGCAGAGTACATGGTAAG21470.10611838996605397No Hit
GTACATGGGAAGCAGTGGTAAGCAG20520.10142288598525512No Hit
GTACATGATAAGCAGTGGTATCAAC20350.1005826379044806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG400.00528621614.2474045
TATTCCG751.481877E-512.664365
GGACCGA550.003074323312.0881096
AGACCGT752.0788706E-411.3973616
CCTATAC1303.8532926E-710.2286533
TGGACCG1409.7166776E-810.1767175
CCGCATT750.002653797610.13073613
AAGACCG951.6474049E-49.9981785
CTATACT2800.09.8374934
TATACTG3700.09.754985
GTTCTAA1901.02591E-99.513321
TTAGACA1804.1945896E-99.498274
CCAGACG800.0045241579.49803453
GTTATCC900.00111592849.49803453
ACACTGT4200.09.49786
GGACCGT800.004525089.49786
GGACCGC1401.0620279E-69.49786
CCCCGAA1502.6602538E-79.49709612
TCTATAC2550.09.3117983
ATAGAAC2054.2382453E-109.2663763
Produced by FastQC (version 0.11.5)