FastQCFastQC Report
Wed 25 May 2016
SRR1294509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294509_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023212
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC99650.4925336544069529No Hit
GTACATGGTAAGCAGTGGTATCAAC91500.452251172887468No Hit
GTATCAACGCAGAGTACTTTTTTTT87580.4328760406719612No Hit
ACCATGTACTCTGCGTTGATACCAC86780.42892193205655166No Hit
GGTATCAACGCAGAGTACTTTTTTT59910.2961133089364832No Hit
GTGGTAAGCAGTGGTATCAACGCAG58130.28731541726719695No Hit
CATGTACTCTGCGTTGATACCACTG55430.2739703006901897No Hit
TATCAACGCAGAGTACTTTTTTTTT53890.26635864160552625No Hit
GGTAAGCAGTGGTATCAACGCAGAG52530.25963665695933No Hit
CCATGTACTCTGCGTTGATACCACT42230.20872750853593197No Hit
GTGGTATCAACGCAGAGTACATGGG40690.2011158494512686No Hit
GAGTACATGGTAAGCAGTGGTATCA35880.17734177140111862No Hit
ACTCTGCGTTGATACCACTGCTTAC33370.16493575562027113No Hit
GTATCAACGCAGAGTACATGGGAAG32620.16122877879332467No Hit
GCTTACCATGTACTCTGCGTTGATA30940.1529251507009646No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30790.15218375533557532No Hit
GCAGTGGTATCAACGCAGAGTACAT29950.14803194128939529No Hit
GCGTTGATACCACTGCTTACCATGT28000.1383938015393345No Hit
GTACTCTGCGTTGATACCACTGCTT27520.13602133637008873No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26730.1321166541123718No Hit
GGTATCAACGCAGAGTACATGGGAA26190.12944763079697036No Hit
GTACATAAGCAGTGGTATCAACGCA26170.12934877808158513No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA25080.12396130509308961No Hit
AAGCAGTGGTATCAACGCAGAGTAC24190.11956235925844647No Hit
GTACATGGGAAGCAGTGGTAAGCAG23040.11387832812379524No Hit
GTATCAACGCAGAGTACATGGTAAG22500.11120930480839379No Hit
GTACATGGGAAGCAGTGGTATCAAC21880.10814487063145137No Hit
CTGCTTACCATGTACTCTGCGTTGA21720.10735404890836946No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21020.10389420386988611No Hit
GTACATGATAAGCAGTGGTATCAAC20950.10354821936603775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG707.2516777E-613.5738655
TACACCG550.003064871512.09308055
GTCCTAG2250.011.4082511
CTTAGAC1001.9255986E-611.4023293
ACGACGG752.0776155E-411.39810219
GTATACG600.00584512911.0913561
GTACGAC600.00586776711.0855983
CGACACG600.00588290111.08176213
TCTACAC2350.010.9171233
AAGAGCG803.7654815E-410.689425
TAGGACA1451.4391844E-810.48494
GTCTAGT1105.99498E-610.3711391
GTATTAA3450.010.1957811
AGCGCTA856.603376E-410.05739814
TACGACG856.604877E-410.05714918
GCCTACG856.604877E-410.05714915
CCTAAGT951.6409939E-410.0020432
GCTCTAA1356.380469E-79.8589831
TTAGAAC1451.6125705E-79.8295953
GTATAGG1554.0186933E-89.8135491
Produced by FastQC (version 0.11.5)