FastQCFastQC Report
Wed 25 May 2016
SRR1294508_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294508_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873337
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC46890.2503020011882539No Hit
ACCATGTACTCTGCGTTGATACCAC40080.21394975917306924No Hit
GTGGTATCAACGCAGAGTACATGGG38520.20562237333699168No Hit
GTACATGGTAAGCAGTGGTATCAAC38430.20514194723106413No Hit
GTGGTAAGCAGTGGTATCAACGCAG33790.1803733124365771No Hit
GTATCAACGCAGAGTACTTTTTTTT30660.1636651600859856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA30320.16185021701914817No Hit
CATGTACTCTGCGTTGATACCACTG25730.1373484856168431No Hit
GGTAAGCAGTGGTATCAACGCAGAG22100.11797129934443189No Hit
GGTATCAACGCAGAGTACTTTTTTT21130.11279337353610162No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACCG550.003072696312.0888865
CTCAGCG600.005886079711.0808889
GCTAGAC1106.0544153E-610.3624563
TAGACAG3000.010.1316395
GTCTAAC1151.010734E-59.9259611
TAGCACC1304.243182E-69.4991724
CCAGGAC2303.6379788E-129.4989193
CTAATCC1002.758666E-49.4989193
TGCACTG3150.09.3476445
GTACAAG2252.5465852E-119.3009931
GTGCTAG1752.6129783E-89.2405971
AGGACAG3400.09.2190475
TTAGGAC1151.1119884E-49.0859223
TGGACTA1151.1126353E-49.0854375
CCGAGGT1054.47849E-49.05045519
ACACTGT3400.08.9394436
CTGATCA1701.6901686E-78.9392049
AAGAGTG2151.0440999E-98.8357325
GTCTTAC1401.0672535E-58.8329231
GTCCTGG2605.456968E-128.7806571
Produced by FastQC (version 0.11.5)