FastQCFastQC Report
Wed 25 May 2016
SRR1294508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294508_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873337
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT101350.5410131759528585No Hit
GGTATCAACGCAGAGTACTTTTTTT70970.3788426748630919No Hit
TATCAACGCAGAGTACTTTTTTTTT57810.3085937020407967No Hit
CCCATGTACTCTGCGTTGATACCAC50560.2698927101744107No Hit
ACCATGTACTCTGCGTTGATACCAC41850.22339813925631108No Hit
GTACATGGTAAGCAGTGGTATCAAC40530.216351889702707No Hit
GTGGTATCAACGCAGAGTACATGGG38340.20466152112513658No Hit
GTGGTAAGCAGTGGTATCAACGCAG37170.19841598174807842No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29840.15928794445420125No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26650.1422595080329914No Hit
CATGTACTCTGCGTTGATACCACTG26150.13959047411117165No Hit
GGTAAGCAGTGGTATCAACGCAGAG24660.1316367530241489No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19910.10628093076686149No Hit
CCATGTACTCTGCGTTGATACCACT19890.1061741694099887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT456.750934E-414.781336
AGCACCG500.001497034913.3031975
GGACAGT1850.011.3000136
TAGGACC1104.954254E-711.2299714
ACTGATC2550.010.8027688
CGGAACG909.534591E-510.55358414
GTAAGAC1856.002665E-1110.2727383
CCTAGAC1606.148184E-910.0961763
TAGGGGT1252.5621684E-69.8823754
CTGATCA2900.09.8265379
GTCTAGC1502.621382E-79.5065981
GTCCAGA2303.6379788E-129.50659751
GTAGGAC1201.6956E-59.5022833
GAGTGTC900.00111346589.5002547
ACTGACC1401.0600434E-69.4989868
GTGTAGG2054.1654857E-109.2747311
CTGGACC2054.2018655E-109.270524
TAGATAG1951.6734703E-99.25863555
GACCATG1856.692062E-99.243497
TCTGGAC1752.658453E-89.2307893
Produced by FastQC (version 0.11.5)