FastQCFastQC Report
Wed 25 May 2016
SRR1294507_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294507_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768937
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC510251.3538300056488075No Hit
ACCATGTACTCTGCGTTGATACCAC413851.0980549688148145No Hit
GTACATGGTAAGCAGTGGTATCAAC394851.0476428764927617No Hit
GTGGTAAGCAGTGGTATCAACGCAG282810.7503707278736684No Hit
CATGTACTCTGCGTTGATACCACTG270840.7186111097107751No Hit
GGTAAGCAGTGGTATCAACGCAGAG240580.6383232195178641No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA208100.5521450743273236No Hit
CCATGTACTCTGCGTTGATACCACT188620.500459413357135No Hit
ACTCTGCGTTGATACCACTGCTTAC168320.4465980726130471No Hit
GTATCAACGCAGAGTACATGGGAAG167030.4431753568711815No Hit
GTACTCTGCGTTGATACCACTGCTT152820.40547241835031994No Hit
GAGTACATGGTAAGCAGTGGTATCA145550.3861831598670925No Hit
GCAGTGGTATCAACGCAGAGTACAT143580.3809562218737007No Hit
GCTTACCATGTACTCTGCGTTGATA133520.3542643456231823No Hit
GCGTTGATACCACTGCTTACCATGT130550.3463841396128404No Hit
GTACATGGGAAGCAGTGGTATCAAC129080.3424838356279237No Hit
GTACATGGGAAGCAGTGGTAAGCAG125670.3334361916901238No Hit
GGTATCAACGCAGAGTACATGGGAA123180.3268295543279179No Hit
GTGGTATCAACGCAGAGTACATGGG122310.3245212111531713No Hit
CTGCTTACCATGTACTCTGCGTTGA111700.29637003749333035No Hit
GCGTTGATACCACTGCTTACCACTG109750.2911961648602776No Hit
GTATCAACGCAGAGTACATGGTAAG108100.28681827263230986No Hit
TATCAACGCAGAGTACATGGGAAGC107870.2862080209884113No Hit
GCTTACCACTGCTTCCCATGTACTC105830.280795354233833No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA103500.27461323975433927No Hit
GTACATGATAAGCAGTGGTATCAAC103450.2744805763534917No Hit
AAGCAGTGGTATCAACGCAGAGTAC103270.2740029881104407No Hit
GTACATAAGCAGTGGTATCAACGCA95950.2545810662263657No Hit
ATCATGTACTCTGCGTTGATACCAC95930.25452800086602667No Hit
ATGGTAAGCAGTGGTATCAACGCAG89140.23651231103093526No Hit
CAGTGGTATCAACGCAGAGTACATG86560.22966687954720388No Hit
TATCAACGCAGAGTACATGGTAAGC85430.22666868668805024No Hit
ACGCAGAGTACATGGTAAGCAGTGG82190.2180720983131318No Hit
ACGCAGAGTACATGGGAAGCAGTGG68570.1819345879222709No Hit
GTGATAAGCAGTGGTATCAACGCAG68220.18100594411633839No Hit
GGTATCAACGCAGAGTACATGGTAA68100.18068755195430436No Hit
TACCACTGCTTACCATGTACTCTGC64750.1717991040975214No Hit
GTGGTATCAACGCAGAGTACATGGT61150.16224733923650092No Hit
GCTTATGTACTCTGCGTTGATACCA58530.15529577703209152No Hit
GTATCAACGCAGAGTACTTTTTTTT54790.14537255464869803No Hit
GCTTCCCATGTACTCTGCGTTGATA53540.14205596962751035No Hit
GAGTACATGGGAAGCAGTGGTAAGC52820.14014561665530625No Hit
GAGTACATGGGAAGCAGTGGTATCA52760.13998642057428926No Hit
GTACATGGTAAGCAGTGGTAAGCAG48720.12926721778581068No Hit
AAAAAGTACTCTGCGTTGATACCAC48160.1277813876963186No Hit
GAGTAAGCAGTGGTATCAACGCAGA46610.12366882227004591No Hit
GCAGAGTACATGGTAAGCAGTGGTA44940.11923786468173918No Hit
TGGTAAGCAGTGGTATCAACGCAGA43940.11658459666478904No Hit
ACTCTGCGTTGATACCACTGCTTAT43640.11578861625970399No Hit
GTAAGCAGTGGTATCAACGCAGAGT43300.11488650513394096No Hit
GATAAGCAGTGGTATCAACGCAGAG42840.11366600184614388No Hit
GCTTACCACTGCTTACCATGTACTC40890.10849212921309112No Hit
GAGTACATGATAAGCAGTGGTATCA39590.10504288079105593No Hit
AAAGTACTCTGCGTTGATACCACTG39390.10451222718766591No Hit
GCGTTGATACCACTGCTTCCCATGT39360.1044326291471574No Hit
GTACATGGGAAGTAAGCAGTGGTAT38630.10249574349478381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA10750.012.8307691
TACGACC1001.937431E-611.3972384
CCTATCC855.3382995E-511.1742073
GTCTTAA2200.010.8096211
ATAGCCG1106.0516995E-610.363616
TAGCGAA2053.6379788E-1210.20090818
GTCCAAT1606.0499588E-910.1069951
TAGATAG1701.5679689E-910.0563865
ACATAAG20900.010.0434213
GTCCTAA1752.5193003E-99.784251
CATAAGC21400.09.76398854
GTCTAAG1951.6007107E-109.7563751
TAAGACA2054.1836756E-119.7293494
TACATAA22200.09.6713942
GTATTAG3250.09.6588121
GTACATA28050.09.5294221
GCTTATG13100.09.51246551
GTGTAGG3000.09.51246551
TACGGGT1002.763897E-49.4976984
GAACCGT900.00111676119.4976986
Produced by FastQC (version 0.11.5)