FastQCFastQC Report
Wed 25 May 2016
SRR1294507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294507_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768937
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC547501.4526642392802003No Hit
ACCATGTACTCTGCGTTGATACCAC436751.1588148064029726No Hit
GTACATGGTAAGCAGTGGTATCAAC408921.0849743574912503No Hit
GTGGTAAGCAGTGGTATCAACGCAG307040.8146594119243702No Hit
CATGTACTCTGCGTTGATACCACTG284520.7549078161826531No Hit
GGTAAGCAGTGGTATCAACGCAGAG258520.6859228477419496No Hit
CCATGTACTCTGCGTTGATACCACT201580.5348457668568087No Hit
ACTCTGCGTTGATACCACTGCTTAC181060.4804007071489919No Hit
GTATCAACGCAGAGTACATGGGAAG177740.4715918573327174No Hit
GTATCAACGCAGAGTACTTTTTTTT172600.4579540597255937No Hit
GTACTCTGCGTTGATACCACTGCTT155560.4127423727167634No Hit
GAGTACATGGTAAGCAGTGGTATCA154370.40958498377659275No Hit
GCAGTGGTATCAACGCAGAGTACAT152670.40507442814777744No Hit
GCTTACCATGTACTCTGCGTTGATA136940.3633385222411518No Hit
GTACATGGGAAGCAGTGGTATCAAC134990.3581646496080991No Hit
GTACATGGGAAGCAGTGGTAAGCAG133830.35508685870843687No Hit
GCGTTGATACCACTGCTTACCATGT131840.3498068553547061No Hit
GGTATCAACGCAGAGTACATGGGAA130120.3452432343655519No Hit
GTGGTATCAACGCAGAGTACATGGG125460.33287900540656423No Hit
AAGCAGTGGTATCAACGCAGAGTAC120780.3204617110872376No Hit
CTGCTTACCATGTACTCTGCGTTGA119450.3169328646246939No Hit
GCGTTGATACCACTGCTTACCACTG115670.3069035115206224No Hit
TATCAACGCAGAGTACATGGGAAGC110930.29432702112027875No Hit
TATCAACGCAGAGTACTTTTTTTTT110550.2933187792738377No Hit
GTATCAACGCAGAGTACATGGTAAG109780.29127576290078605No Hit
GCTTACCACTGCTTCCCATGTACTC108910.28896741972603945No Hit
GTACATGATAAGCAGTGGTATCAAC107150.2842976680162072No Hit
GGTATCAACGCAGAGTACTTTTTTT105100.27885846858145946No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA100660.26707795858620087No Hit
ATCATGTACTCTGCGTTGATACCAC99090.2629123277995891No Hit
ATGGTAAGCAGTGGTATCAACGCAG98100.26028559246280847No Hit
GTACATAAGCAGTGGTATCAACGCA96200.2552443832306032No Hit
GTACTTTTTTTTTTTTTTTTTTTTT93890.24911533411144843No Hit
CAGTGGTATCAACGCAGAGTACATG93730.24869081122873637No Hit
ACGCAGAGTACATGGTAAGCAGTGG90040.23890025224619038No Hit
TATCAACGCAGAGTACATGGTAAGC89010.23616738618873176No Hit
ACGCAGAGTACATGGGAAGCAGTGG76120.20196676145024448No Hit
GTGATAAGCAGTGGTATCAACGCAG75800.2011177156848204No Hit
GGTATCAACGCAGAGTACATGGTAA68850.18267750296701696No Hit
TACCACTGCTTACCATGTACTCTGC66860.17739749961328619No Hit
GTGGTATCAACGCAGAGTACATGGT59960.15908995029633022No Hit
GCTTATGTACTCTGCGTTGATACCA59000.1565428130000581No Hit
GAGTACTTTTTTTTTTTTTTTTTTT58870.1561978881578546No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58260.15457939466751502No Hit
GAGTACATGGGAAGCAGTGGTATCA56810.1507321560429373No Hit
GCTTCCCATGTACTCTGCGTTGATA55550.14738903834158013No Hit
GAGTACATGGGAAGCAGTGGTAAGC53620.14226823106886635No Hit
GTACATGGTAAGCAGTGGTAAGCAG51050.1354493322653045No Hit
GAGTAAGCAGTGGTATCAACGCAGA49290.13077958055547229No Hit
GCAGAGTACATGGTAAGCAGTGGTA49120.13032852499259073No Hit
GATAAGCAGTGGTATCAACGCAGAG48330.12823244325920014No Hit
AAAAAGTACTCTGCGTTGATACCAC47570.12621595956631804No Hit
GTAAGCAGTGGTATCAACGCAGAGT46670.1238280183510629No Hit
TGGTAAGCAGTGGTATCAACGCAGA46100.12231565558140133No Hit
ACTCTGCGTTGATACCACTGCTTAT45580.12093595621258726No Hit
GAGTACATGATAAGCAGTGGTATCA41590.1103494168249562No Hit
GCTTACCACTGCTTACCATGTACTC40860.10841253117258262No Hit
GTACATGGGAAGTAAGCAGTGGTAT40720.10804107365020961No Hit
CTTCCCATGTACTCTGCGTTGATAC40240.10676750500207353No Hit
AAAGTACTCTGCGTTGATACCACTG39860.10575926315563249No Hit
GTGGTATCAACGCAGAGTACTTTTT39780.10554700171427646No Hit
GCGTTGATACCACTGCTTCCCATGT39760.10549393635393746No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT39140.10384891018342837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG655.4490105E-513.1557755
TACGACC1352.7284841E-1112.6688614
CGTCTAG802.8735582E-511.87311819
TCGCTAA657.9825736E-411.7002331
GTACGAC658.016425E-411.6943333
GTCCTAG1406.7484507E-1011.5435351
CACGATT951.3633022E-510.99869414
ACCGCGC803.776114E-410.6866578
TAGGCCC2100.010.4065644
TCCTAGG1856.002665E-1110.2724582
CTACGAC1409.6773874E-810.1803353
ACGACCG951.6428017E-410.0014675
CCCTAGG2002.5465852E-119.9817621
CCCATAA1356.3907646E-79.858531
TAACACT3250.09.6478254
GTACTAA2106.548362E-119.5064391
CCTAGGC1701.661465E-89.5020242
CTACACT3000.09.5016464
CCTAGGG1201.6983957E-59.5016453
GTCTTTA2950.09.3453141
Produced by FastQC (version 0.11.5)