FastQCFastQC Report
Wed 25 May 2016
SRR1294506_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294506_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010944
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC112600.5599360300436014No Hit
GTACATGGTAAGCAGTGGTATCAAC85940.42736147799242546No Hit
ACCATGTACTCTGCGTTGATACCAC82160.4085643359536616No Hit
GTGGTAAGCAGTGGTATCAACGCAG75250.3742023646605773No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA66490.3306407339040769No Hit
CATGTACTCTGCGTTGATACCACTG57420.2855375385888419No Hit
GGTAAGCAGTGGTATCAACGCAGAG52230.2597287642022851No Hit
GTGGTATCAACGCAGAGTACATGGG43080.21422774577511855No Hit
CCATGTACTCTGCGTTGATACCACT36730.18265053626555489No Hit
ACTCTGCGTTGATACCACTGCTTAC36130.17966686292606857No Hit
GTATCAACGCAGAGTACATGGGAAG35670.17737938003246237No Hit
GTACATGGGAAGCAGTGGTAAGCAG34160.1698704687947551No Hit
GCTTACCATGTACTCTGCGTTGATA32270.16047189777537316No Hit
GCAGTGGTATCAACGCAGAGTACAT31880.15853251010470706No Hit
GCTTACCACTGCTTCCCATGTACTC31290.15559856465421215No Hit
GGTATCAACGCAGAGTACATGGGAA30090.14963121797523948No Hit
GAGTACATGGTAAGCAGTGGTATCA29970.14903448330734223No Hit
GTACTCTGCGTTGATACCACTGCTT27090.13471285127780785No Hit
GTACATGGGAAGCAGTGGTATCAAC27040.13446421183285062No Hit
GTACATAAGCAGTGGTATCAACGCA25990.12924278348874957No Hit
GCGTTGATACCACTGCTTACCATGT25690.1277509468190064No Hit
GTATCAACGCAGAGTACTTTTTTTT25060.12461808981254574No Hit
GTACATGATAAGCAGTGGTATCAAC24290.12078904236020496No Hit
TATCAACGCAGAGTACATGGGAAGC23560.11715890646382993No Hit
GAGTAAGCAGTGGTATCAACGCAGA22460.11168883867477164No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA22100.10989863467107984No Hit
GCGTTGATACCACTGCTTACCACTG21620.10751169599949079No Hit
AAGCAGTGGTATCAACGCAGAGTAC20970.10427938321504726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC1207.421477E-1012.6649753
GTCTTAG1552.5465852E-1111.6610111
TAGGACT2300.011.1504084
TAGACAG2750.010.3614795
GTCTTAC1752.5138434E-99.7847271
GTCCCAC1856.330083E-109.7700361
GTTCTAT1804.08545E-99.5129291
GCTTATG5100.09.5129291
CTTAGAC1304.2462634E-69.4987323
GTGTTAG3150.09.3619311
CCAGGAC2451.8189894E-129.304883
GTATTAT3900.09.2690091
CCTACAC1951.6862032E-99.2551743
TTAGACA2700.09.1466994
TAGCACC1151.11263624E-49.0855184
CTAATAC2106.693881E-109.0464113
ACACCGT1054.502269E-49.0457356
CTAGGGG850.0074444938.9399823
GACTGTC1701.6901868E-78.9393167
GTATTAA3950.08.9108451
Produced by FastQC (version 0.11.5)