FastQCFastQC Report
Wed 25 May 2016
SRR1294506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010944
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC121950.6064316062505968No Hit
GTACATGGTAAGCAGTGGTATCAAC90850.45177787148722187No Hit
ACCATGTACTCTGCGTTGATACCAC89560.44536297380732626No Hit
GTGGTAAGCAGTGGTATCAACGCAG85040.42288596798319594No Hit
GTATCAACGCAGAGTACTTTTTTTT80930.4024478056077146No Hit
CATGTACTCTGCGTTGATACCACTG61180.30423522484962284No Hit
GGTAAGCAGTGGTATCAACGCAGAG57940.2881233888163967No Hit
GGTATCAACGCAGAGTACTTTTTTT56550.2812112122465867No Hit
TATCAACGCAGAGTACTTTTTTTTT49820.24774434295534833No Hit
GTGGTATCAACGCAGAGTACATGGG43810.21785788167149356No Hit
CCATGTACTCTGCGTTGATACCACT40170.19975693007860984No Hit
ACTCTGCGTTGATACCACTGCTTAC38670.19229774672989403No Hit
GTATCAACGCAGAGTACATGGGAAG38630.19209883517392826No Hit
GTACATGGGAAGCAGTGGTAAGCAG36720.18260080837656345No Hit
GCTTACCATGTACTCTGCGTTGATA33610.16713543490022598No Hit
GCAGTGGTATCAACGCAGAGTACAT32820.1632069316699023No Hit
GAGTACATGGTAAGCAGTGGTATCA32810.16315720378091084No Hit
GCTTACCACTGCTTCCCATGTACTC32600.16211291811209064No Hit
GGTATCAACGCAGAGTACATGGGAA32570.16196373444511633No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30760.1529629865376659No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA30380.15107332675599122No Hit
GTACATGGGAAGCAGTGGTATCAAC28830.1433655039623182No Hit
GTACTCTGCGTTGATACCACTGCTT27550.13700033417141402No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27450.13650305528149964No Hit
GTACATAAGCAGTGGTATCAACGCA26750.1330221030520989No Hit
GCGTTGATACCACTGCTTACCATGT25760.12809904204194647No Hit
AAGCAGTGGTATCAACGCAGAGTAC25500.12680611692816907No Hit
TATCAACGCAGAGTACATGGGAAGC24870.12367325992170841No Hit
GTACATGATAAGCAGTGGTATCAAC24260.12063985869323064No Hit
GAGTAAGCAGTGGTATCAACGCAGA22900.11387686579039495No Hit
GCGTTGATACCACTGCTTACCACTG21730.10805870277839662No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21520.10701441710957639No Hit
CTGCTTACCATGTACTCTGCGTTGA21300.10592040355176474No Hit
ATCATGTACTCTGCGTTGATACCAC21290.1058706756627733No Hit
GTATCAACGCAGAGTACATGGTAAG21290.1058706756627733No Hit
ATGGTAAGCAGTGGTATCAACGCAG20690.10288700232328697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACTG2650.010.398735
CGTATAC750.002642863210.1357753
AGACCTA856.57714E-410.0617486
CTAAGAC1651.0180884E-89.7902363
GTTCTAG1951.6189006E-109.7512741
TCTTAGA1951.6370905E-109.7459372
TAGGACA1951.6370905E-109.7459374
GTCTTGC2401.8189894E-129.5074921
AATACTG2600.09.5022895
GTATTGG2357.2759576E-129.3052041
CTCTTAG1951.6589183E-99.2637111
CTAGCAC1651.06168955E-79.214343
ACTGTCC1451.7070943E-69.1721138
CTAGGAC1252.7464444E-59.1221973
AGCACTG4400.09.0703665
TTACCCC950.00181994959.0021694
GGACTGT2750.08.9844296
CCCTTAC1804.1645762E-88.9792981
GTCTAGC850.0073884138.9482281
GCTTATG4150.08.93475151
Produced by FastQC (version 0.11.5)