FastQCFastQC Report
Wed 25 May 2016
SRR1294505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118491
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC54260.2561257045699038No Hit
ACCATGTACTCTGCGTTGATACCAC41120.19410042336738745No Hit
GTACATGGTAAGCAGTGGTATCAAC38270.18064745141707No Hit
GTGGTAAGCAGTGGTATCAACGCAG30110.14212946856984524No Hit
CATGTACTCTGCGTTGATACCACTG28050.13240556603733505No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA26730.12617471587087223No Hit
GTGGTATCAACGCAGAGTACATGGG24930.11767810200751384No Hit
GGTAAGCAGTGGTATCAACGCAGAG22210.10483877439177226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCA508.7399094E-515.1951859
CACGCTC400.005291053614.2454839
TAGTACG1350.014.0722722
TACGGGG707.2857893E-613.5677685
AGTACGG1700.013.4075163
GTACGGG1052.0030711E-812.6629524
ATCCGGA604.1032548E-412.66295111
CGTACCA701.09473534E-412.21012710
TATTCCG951.043747E-611.9967635
AAGTACG600.005882453611.0819152
TCGTGGG1355.7143552E-810.55246112
ACACCGT750.002654710.1303626
GTACGGT750.002654710.1303624
CGAACTC856.602813E-410.05753615
GTATTAC2600.09.877651
GTTCTAG2550.09.6983181
TGGACTG3050.09.6531335
TAGACAG3450.09.6350825
CGTGGGG1401.0623371E-69.49766313
TTAGGAC1502.6609814E-79.4969893
Produced by FastQC (version 0.11.5)