FastQCFastQC Report
Wed 25 May 2016
SRR1294505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118491
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC56900.2685874049028294No Hit
GTATCAACGCAGAGTACTTTTTTTT54550.2574946034701115No Hit
ACCATGTACTCTGCGTTGATACCAC45200.2133594147909998No Hit
GTACATGGTAAGCAGTGGTATCAAC40010.18886084481831644No Hit
GTGGTAAGCAGTGGTATCAACGCAG34870.164598291897393No Hit
GGTATCAACGCAGAGTACTTTTTTT34350.16214371455908946No Hit
TATCAACGCAGAGTACTTTTTTTTT31550.14892675966053195No Hit
CATGTACTCTGCGTTGATACCACTG30300.14302633336653306No Hit
GGTAAGCAGTGGTATCAACGCAGAG25570.12069912027004127No Hit
GTGGTATCAACGCAGAGTACATGGG25120.11857496680420167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC1155.304173E-912.3950623
CTAGGAC1553.110472E-1011.0356043
TAGGACT2700.010.5587574
CTAGAAC1856.002665E-1110.2733853
GTATTGC1409.599171E-810.1852661
GTCTTGC3050.09.9737691
GGTTAGA1151.0187108E-59.9195631
CTAGACT1804.1618478E-99.5028824
TTGCACA2900.09.5028814
CCAGTAC2106.548362E-119.5028813
CTAGGGC1606.60566E-89.5028813
TAATACT5050.09.40879254
AGGACAG3300.09.2149155
TGTACTG3200.09.2059155
CCAGGAC2600.09.1373853
CCTAGAC1252.7446427E-59.12276653
TATTATA6050.08.9533392
GTATTAG3300.08.9301121
GTCTAAC1401.0762271E-58.827231
TAGACTG2800.08.8241035
Produced by FastQC (version 0.11.5)