FastQCFastQC Report
Wed 25 May 2016
SRR1294504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540122
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC42800.27790006246258414No Hit
GTGGTAAGCAGTGGTATCAACGCAG31510.20459418149990716No Hit
ACCATGTACTCTGCGTTGATACCAC31300.20323065315604869No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA30910.2006983862317401No Hit
GTACATGGTAAGCAGTGGTATCAAC30910.2006983862317401No Hit
GTGGTATCAACGCAGAGTACATGGG23030.14953360837647928No Hit
CATGTACTCTGCGTTGATACCACTG22340.14505344381808713No Hit
ATTCCATTCCATTCCATTCCATTCC17740.11518568009547296No Hit
GGTAAGCAGTGGTATCAACGCAGAG17400.11297806277684495No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCG400.00528596314.2471997
TATTCCG658.034402E-411.6903895
CTTAGGC700.001493027310.8557143
CTTGGAC1252.2433596E-710.6385993
CCAGGGG1659.313226E-1010.3622723
ATAGAAC2700.010.202363
CTACACC1409.697578E-810.1775624
TAGACAG2250.010.1316715
CTACACT2550.010.0578264
CGAGTGC856.608739E-410.05619310
GTACTAG951.628269E-410.0094111
CTAGACA1356.448372E-79.8508754
TAGGACA1752.575689E-99.770464
TTGGACA1856.493792E-109.755794
TCTACAC1856.493792E-109.7554733
TATACTG3800.09.74845
CTATACT2054.1836756E-119.7307424
TGGACTG2550.09.6846865
ACCTTAC800.00448050359.5089411
GACTGTG2600.09.4981337
Produced by FastQC (version 0.11.5)