FastQCFastQC Report
Wed 25 May 2016
SRR1294504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540122
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC45430.2949766317213831No Hit
GTATCAACGCAGAGTACTTTTTTTT44680.29010688763617426No Hit
GTGGTAAGCAGTGGTATCAACGCAG35140.2281637428723179No Hit
GTACATGGTAAGCAGTGGTATCAAC33260.21595691769872777No Hit
GGTATCAACGCAGAGTACTTTTTTT33150.21524268856623047No Hit
ACCATGTACTCTGCGTTGATACCAC32560.21141182321919952No Hit
TATCAACGCAGAGTACTTTTTTTTT26190.1700514634554925No Hit
GTGGTATCAACGCAGAGTACATGGG24490.15901337686235248No Hit
CATGTACTCTGCGTTGATACCACTG23490.1525203847487407No Hit
GGTAAGCAGTGGTATCAACGCAGAG19860.12895082337632993No Hit
ATTCCATTCCATTCCATTCCATTCC18530.12031514386522627No Hit
GTATCAACGCAGAGTACATGGGAAG16310.10590070137300811No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15480.10051151791871032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCT751.4824125E-512.66346419
CTCGATC802.87375E-511.87199718
GTCTAGA1459.640644E-1111.8023831
GTCCTAG1752.0008883E-1110.8656851
TATACTG3150.010.5583715
CCTATAC1803.45608E-1110.5583713
TCTCGAT909.535317E-510.55322817
GTCTTAT1451.5977275E-79.83531951
TCTGGAC1304.2223055E-69.5025343
TAGAACT2401.8189894E-129.5025334
CGAACTC800.0045224189.49821515
CTTGTAC1951.6716513E-99.258883
GTTCTAG1651.05193976E-79.2193691
CTAATAC1651.0603799E-79.2145783
CTCTTAG1451.6871181E-69.1796311
GTCTAAG1451.6871181E-69.1796311
CTAGGAC1356.8207028E-69.1505883
GTCCTGG2501.8189894E-129.1271761
GTGTAAG2501.8189894E-129.1271761
ACCTTAC1054.456216E-49.0547381
Produced by FastQC (version 0.11.5)