FastQCFastQC Report
Wed 25 May 2016
SRR1294503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850803
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC116500.3025343025857204No Hit
ACCATGTACTCTGCGTTGATACCAC79720.2070217562414904No Hit
GTACATGGTAAGCAGTGGTATCAAC75340.19564750520865387No Hit
GTGGTAAGCAGTGGTATCAACGCAG59630.15485081942649365No Hit
CATGTACTCTGCGTTGATACCACTG57400.14905981947141933No Hit
GTGGTATCAACGCAGAGTACATGGG54830.1423858867877687No Hit
GGTAAGCAGTGGTATCAACGCAGAG49350.12815508869189102No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43960.11415800808298945No Hit
GTATCAACGCAGAGTACATGGGAAG39670.103017474537129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG5300.09.5137961
TAGACTG4100.09.497985
GTCTTAT3750.09.1332441
TCTTATA5550.09.0736792
GCACCGT1951.6574631E-88.7672536
TTTAGAC4350.08.7340023
CTAGCAC2304.0563464E-108.6722953
TCTAGAC2751.8189894E-128.6347523
GTATAGG3550.08.5758161
GTGCTAA3400.08.3945251
TAGACAG5350.08.3440215
GTAGGAC2409.367795E-108.3109493
GTATAAA7500.08.1184391
GTCCTAT2701.2914825E-108.1043441
TAGGACT3900.08.0369624
TAGGACC3350.07.93881654
TAGGGGC2053.4363438E-77.87657834
GTGTAAA5200.07.86717751
GTCCTAG2802.8012437E-107.81490331
CTAATAC4450.07.6839593
Produced by FastQC (version 0.11.5)