FastQCFastQC Report
Wed 25 May 2016
SRR1294503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850803
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC122440.31795965672614257No Hit
GTATCAACGCAGAGTACTTTTTTTT110550.2870829798356343No Hit
ACCATGTACTCTGCGTTGATACCAC82590.2144747472150614No Hit
GTACATGGTAAGCAGTGGTATCAAC74580.1936738908741891No Hit
TATCAACGCAGAGTACTTTTTTTTT66500.17269125426566875No Hit
GGTATCAACGCAGAGTACTTTTTTT66270.1720939762433965No Hit
GTGGTAAGCAGTGGTATCAACGCAG64340.16708203457824253No Hit
CATGTACTCTGCGTTGATACCACTG58170.15105940241554813No Hit
GGTAAGCAGTGGTATCAACGCAGAG54750.14217813791045658No Hit
GTGGTATCAACGCAGAGTACATGGG54320.14106148769490415No Hit
GTATCAACGCAGAGTACATGGGAAG41100.10673098571908249No Hit
CCATGTACTCTGCGTTGATACCACT40620.10548449245521No Hit
ACTCTGCGTTGATACCACTGCTTAC39370.10223841624720871No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAA400.005282751814.24927910
TCGGTAA550.00307192112.08982713
GACCGTC951.3611185E-511.00037
AAACCGT1158.808147E-710.7409586
GTATAGG3200.010.396311
TATGCGT856.600729E-410.05831410
TAGACAG5450.09.7630025
AGGGTCG900.00111269959.5014935
AACCGTG1151.1110922E-49.0872047
ACCGAGT950.00182535698.9996628
GGACCGT1502.6980942E-68.8681766
GTGCTAG3150.08.4490641
CTAGACA3400.08.3836714
GTCCTAG2951.8189894E-128.3774621
TCTATAC3300.08.3497973
ATATCGT1050.00450806938.1442436
CTAGGAC3150.08.1441373
CAGCGAA1050.00451651268.1425519
GTATTAA6600.08.0650161
TCTAGAC3300.08.0618733
Produced by FastQC (version 0.11.5)