FastQCFastQC Report
Wed 25 May 2016
SRR1294502_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294502_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713517
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC134051.8787218804877808No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA120221.6848932821502502No Hit
ACCATGTACTCTGCGTTGATACCAC81021.1355020272817606No Hit
GTACATGGTAAGCAGTGGTATCAAC75791.062203143022521No Hit
CATGTACTCTGCGTTGATACCACTG72321.0135708049002337No Hit
GTGGTAAGCAGTGGTATCAACGCAG64120.8986471240348863No Hit
GGTAAGCAGTGGTATCAACGCAGAG55030.7712500192707391No Hit
GTATCAACGCAGAGTACATGGGAAG46220.6477771377556526No Hit
GGTATCAACGCAGAGTACATGGGAA44830.628296172340673No Hit
ACTCTGCGTTGATACCACTGCTTAC40720.5706941810776758No Hit
GTACTCTGCGTTGATACCACTGCTT40010.5607434721247007No Hit
CCATGTACTCTGCGTTGATACCACT39130.5484101990562243No Hit
GTGGTATCAACGCAGAGTACATGGG37220.5216413904644178No Hit
GTACATGGGAAGCAGTGGTAAGCAG33240.4658613599956273No Hit
TATCAACGCAGAGTACATGGGAAGC32460.45492959523038695No Hit
GCAGTGGTATCAACGCAGAGTACAT31800.44567964042902974No Hit
GCTTACCACTGCTTCCCATGTACTC29650.415547211909457No Hit
GTACATGGGAAGCAGTGGTATCAAC29180.40896012288424805No Hit
GAGTACATGGTAAGCAGTGGTATCA28900.4050358996351874No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA28310.3967670006460954No Hit
AAGCAGTGGTATCAACGCAGAGTAC27500.3854147833898842No Hit
GCGTTGATACCACTGCTTACCACTG27440.38457387840794266No Hit
GCTTACCATGTACTCTGCGTTGATA27370.38359282259567745No Hit
GTACATAAGCAGTGGTATCAACGCA26200.36719517544781693No Hit
GTACATGATAAGCAGTGGTATCAAC25290.3544414498883699No Hit
ATCATGTACTCTGCGTTGATACCAC25110.35191873494254516No Hit
GCGTTGATACCACTGCTTACCATGT24930.3493960199967205No Hit
CTGCTTACCATGTACTCTGCGTTGA23340.327112037975269No Hit
ACGCAGAGTACATGGGAAGCAGTGG20030.2807221131381593No Hit
CAGTGGTATCAACGCAGAGTACATG19980.28002135898654135No Hit
TATCAACGCAGAGTACATGGTAAGC19350.2711918566761549No Hit
GTATCAACGCAGAGTACATGGTAAG19110.2678282367483886No Hit
GTATCAACGCAGAGTACTTTTTTTT17540.24582455638758433No Hit
ACGCAGAGTACATGGTAAGCAGTGG17510.24540410389661355No Hit
ATGGTAAGCAGTGGTATCAACGCAG17240.2416200314778765No Hit
AAAAAGTACTCTGCGTTGATACCAC15600.21863529530480705No Hit
GGTATCAACGCAGAGTACATGGTAA15440.21639288201962953No Hit
GCTTATGTACTCTGCGTTGATACCA15280.214150468734452No Hit
GTGATAAGCAGTGGTATCAACGCAG14660.20546111725438915No Hit
GAGTACATGGGAAGCAGTGGTAAGC13600.19060512924008818No Hit
GTGGTATCAACGCAGAGTACATGGT12780.17911276115355346No Hit
TATCAACGCAGAGTACTTTTTTTTT12700.1779915545109647No Hit
AAAGTACTCTGCGTTGATACCACTG12640.17715064952902312No Hit
TACCACTGCTTACCATGTACTCTGC12630.17701049869869953No Hit
GAGTACATGGGAAGCAGTGGTATCA12550.17588929205611079No Hit
ACTCTGCGTTGATACCACTGCTTAT11960.16762039306701873No Hit
GGTATCAACGCAGAGTACTTTTTTT11520.16145375653278057No Hit
GATAAGCAGTGGTATCAACGCAGAG10980.15388561169530648No Hit
CTTCCCATGTACTCTGCGTTGATAC10720.15024169010689303No Hit
CTGCTTACCACTGCTTCCCATGTAC10590.14841972931268632No Hit
GAGTACATGATAAGCAGTGGTATCA10450.146457617688156No Hit
CTTATGTACTCTGCGTTGATACCAC10340.14491595855459646No Hit
GTAAGCAGTGGTATCAACGCAGAGT10260.1437947519120077No Hit
AAACAAAAAAAAAAAAAAAAAAAAA10160.14239324360877176No Hit
GCAGAGTACATGGTAAGCAGTGGTA9970.1397303778326235No Hit
TGGTAAGCAGTGGTATCAACGCAGA9750.1366470595655044No Hit
GCTTCCCATGTACTCTGCGTTGATA9650.13524555126226845No Hit
GCTTATCATGTACTCTGCGTTGATA9020.12641604895188202No Hit
GCGTTGATACCACTGCTTCCCATGT8940.12529484230929328No Hit
GAACAAAAAAAAAAAAAAAAAAAAA8410.11786684830214277No Hit
CTGCTTCCCATGTACTCTGCGTTGA8360.1171660941505248No Hit
GATACCACTGCTTACCATGTACTCT8210.11506383169567087No Hit
GAGTAAGCAGTGGTATCAACGCAGA8090.11338202173178774No Hit
GTTGATACCACTGCTTACCATGTAC7940.11127975927693384No Hit
GTACATGGGAAGTAAGCAGTGGTAT7820.10959794931305071No Hit
GTTGATACCACTGCTTACCACTGCT7660.10735553602787319No Hit
GCGTTGATACCACTGCTTATCATGT7650.1072153851975496No Hit
ATACCACTGCTTACCATGTACTCTG7200.10090859783298785No Hit
TATGTACTCTGCGTTGATACCACTG7190.10076844700266427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTT250.006040394318.9937615
GGTCGGA350.002153138616.30322317
GAAGTAT350.002171379716.2826515
AACTCTT350.002174432416.2792267
AGTATGA400.00523791714.2653217
TTACACC400.00528690414.2453214
GTGTTAA551.937734E-413.8349791
GAACAAA2800.013.5879271
CTGCTAA1009.8661985E-913.3161691
CTGGTTA701.0933654E-412.209429
CCCTGTA550.003066748612.0903292
CTTACAC550.003071584312.0877873
CAAGGTA550.003073197612.086944
GTACAGG752.0475747E-411.4138581
TACATAA5500.011.0540152
ACATAAG5450.010.9788153
TACAAGG700.001490284910.8566222
CATAAGC5550.010.7802434
GCAAAAC1355.563379E-810.5683871
GCTTAAC909.381298E-510.5683871
Produced by FastQC (version 0.11.5)