FastQCFastQC Report
Wed 25 May 2016
SRR1294502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713517
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC138171.9364640225811018No Hit
ACCATGTACTCTGCGTTGATACCAC83531.1706798856929828No Hit
GTACATGGTAAGCAGTGGTATCAAC81291.1392860997004977No Hit
CATGTACTCTGCGTTGATACCACTG75511.0582789197734601No Hit
GTGGTAAGCAGTGGTATCAACGCAG74191.0397790101707458No Hit
GGTAAGCAGTGGTATCAACGCAGAG62860.8809881194141134No Hit
GTATCAACGCAGAGTACTTTTTTTT61530.8623480589810755No Hit
GTATCAACGCAGAGTACATGGGAAG50450.7070609389825331No Hit
GTACTTTTTTTTTTTTTTTTTTTTT48670.6821140911849332No Hit
GGTATCAACGCAGAGTACATGGGAA48460.6791709237481377No Hit
TATCAACGCAGAGTACTTTTTTTTT44620.6253530049038776No Hit
ACTCTGCGTTGATACCACTGCTTAC43390.6081144527740755No Hit
GGTATCAACGCAGAGTACTTTTTTT41680.5841486607887408No Hit
CCATGTACTCTGCGTTGATACCACT41350.5795236833880623No Hit
GTACTCTGCGTTGATACCACTGCTT40050.561304075445995No Hit
GTGGTATCAACGCAGAGTACATGGG39210.5495314056988131No Hit
TATCAACGCAGAGTACATGGGAAGC36450.510849776529501No Hit
GTACATGGGAAGCAGTGGTAAGCAG35600.4989369559519955No Hit
AAGCAGTGGTATCAACGCAGAGTAC34720.4866036828835192No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA34690.48618323039254846No Hit
GCAGTGGTATCAACGCAGAGTACAT33740.47286890151180705No Hit
GTACATGGGAAGCAGTGGTATCAAC30880.43278576403925906No Hit
GAGTACATGGTAAGCAGTGGTATCA30440.4266191275050209No Hit
GCTTACCACTGCTTCCCATGTACTC29990.42031234014045915No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27400.3840132750866483No Hit
GCGTTGATACCACTGCTTACCACTG27300.3826117667834123No Hit
GCTTACCATGTACTCTGCGTTGATA26610.3729413594910843No Hit
GTACATGATAAGCAGTGGTATCAAC26480.37111939869687754No Hit
ATCATGTACTCTGCGTTGATACCAC26370.369577739563318No Hit
GTACATAAGCAGTGGTATCAACGCA25350.35528235487031146No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24740.34673315422057216No Hit
GCGTTGATACCACTGCTTACCATGT24660.3456119475779834No Hit
CTGCTTACCATGTACTCTGCGTTGA23920.33524078613403746No Hit
ACGCAGAGTACATGGGAAGCAGTGG22930.3213658539320016No Hit
CAGTGGTATCAACGCAGAGTACATG21890.3067901675783478No Hit
GTATCAACGCAGAGTACATGGTAAG20860.2923546320550176No Hit
TATCAACGCAGAGTACATGGTAAGC20260.28394558223560196No Hit
ATGGTAAGCAGTGGTATCAACGCAG20100.2817031689504244No Hit
ACGCAGAGTACATGGTAAGCAGTGG19860.2783395490226582No Hit
GTGATAAGCAGTGGTATCAACGCAG16600.23265037833716645No Hit
GGTATCAACGCAGAGTACATGGTAA16330.2288663059184294No Hit
AAAAAGTACTCTGCGTTGATACCAC16170.22662389263325192No Hit
GTGGTATCAACGCAGAGTACTTTTT15860.2222792168932205No Hit
GCTTATGTACTCTGCGTTGATACCA15380.21555197703768797No Hit
GAGTACATGGGAAGCAGTGGTAAGC15370.21541182620736435No Hit
TACCACTGCTTACCATGTACTCTGC13360.18724150931232192No Hit
AAAGTACTCTGCGTTGATACCACTG13290.18626045350005677No Hit
GTGGTATCAACGCAGAGTACATGGT13280.18612030266973317No Hit
GAGTACATGGGAAGCAGTGGTATCA13090.18345743689358487No Hit
ACTCTGCGTTGATACCACTGCTTAT12640.17715064952902312No Hit
GATAAGCAGTGGTATCAACGCAGAG12060.16902190137025466No Hit
GTAAGCAGTGGTATCAACGCAGAGT11860.16621888476378277No Hit
CTTCCCATGTACTCTGCGTTGATAC10960.1536053100346593No Hit
GCTTCCCATGTACTCTGCGTTGATA10920.15304470671336493No Hit
TGGTAAGCAGTGGTATCAACGCAGA10730.15038184093721663No Hit
CTGCTTACCACTGCTTCCCATGTAC10710.15010153927656944No Hit
GCAGAGTACATGGTAAGCAGTGGTA10670.14954093595527507No Hit
CTTATGTACTCTGCGTTGATACCAC10590.14841972931268632No Hit
GAGTACATGATAAGCAGTGGTATCA10250.1436546010816841No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9860.13818871869906393No Hit
GCGTTGATACCACTGCTTCCCATGT9490.13300313797709093No Hit
GCTTATCATGTACTCTGCGTTGATA9300.13034027220094266No Hit
GAGTAAGCAGTGGTATCAACGCAGA9120.12781755725511795No Hit
CTGCTTCCCATGTACTCTGCGTTGA8410.11786684830214277No Hit
GCAGTGGTATCAACGCAGAGTACTT8300.11632518916858323No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA8240.11548428418664165No Hit
GCTAAGCAGTGGTATCAACGCAGAG8220.11520398252599447No Hit
GTACATGGGAAGTAAGCAGTGGTAT7990.1119805134285518No Hit
GTTGATACCACTGCTTACCATGTAC7930.11113960844661025No Hit
GATACCACTGCTTACCATGTACTCT7920.11099945761628666No Hit
GTACATGGTAAGCAGTGGTAAGCAG7880.11043885429499227No Hit
GTTGATACCACTGCTTACCACTGCT7770.10889719516143273No Hit
ATCAACGCAGAGTACATGGGAAGCA7740.10847674267046195No Hit
AAGCAGTGGTAAGCAGTGGTATCAA7680.10763583768852039No Hit
ATACCACTGCTTACCATGTACTCTG7530.10553357523366647No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT7470.1046926702517249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAC508.693012E-515.2021313
GTACAAC400.00525580114.2579961
CCTATGT400.00525580114.2579961
GGTCGGA400.005280307514.24800317
GCACTGT707.2388884E-613.5733316
CACTGTA500.001498161313.2999997
CACAACG655.4480086E-513.1529259
TACACAG604.0823748E-412.6684435
GTACAAG1001.4276884E-712.3569281
GGACTGT701.08788125E-412.2159986
ACTGAAT550.003067258512.0900618
ATGACGA550.003067258512.09006111
TGCACTG802.8571667E-511.8766655
GAACAAA1701.8189894E-1211.74187851
CCGCTTC658.018804E-411.6914889
CGCTTCC658.018804E-411.69148810
TACAAGG855.30569E-511.1780382
AAGACCC600.005864257511.08488755
CACTCTT600.005864257511.08488755
TACACAA600.005864257511.08488752
Produced by FastQC (version 0.11.5)