FastQCFastQC Report
Wed 25 May 2016
SRR1294501_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294501_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500807
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC148490.9894010355761934No Hit
ACCATGTACTCTGCGTTGATACCAC99970.6661083004010508No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA98690.657579555532457No Hit
GTACATGGTAAGCAGTGGTATCAAC85890.5722921068465165No Hit
CATGTACTCTGCGTTGATACCACTG76280.5082598895127755No Hit
GTGGTAAGCAGTGGTATCAACGCAG73600.49040282994415674No Hit
GGTAAGCAGTGGTATCAACGCAGAG65010.43316695617757645No Hit
GTATCAACGCAGAGTACATGGGAAG54410.36253828773453217No Hit
ACTCTGCGTTGATACCACTGCTTAC52430.34934538551592575No Hit
CCATGTACTCTGCGTTGATACCACT50440.3360858524780335No Hit
GGTATCAACGCAGAGTACATGGGAA48180.32102728731942215No Hit
GTGGTATCAACGCAGAGTACATGGG47600.3171626998008405No Hit
GTACTCTGCGTTGATACCACTGCTT44170.29430832878577995No Hit
GCAGTGGTATCAACGCAGAGTACAT38200.2545297296721031No Hit
TATCAACGCAGAGTACATGGGAAGC36910.24593435398422314No Hit
GTACATGGGAAGCAGTGGTAAGCAG32500.21655016267914529No Hit
GCGTTGATACCACTGCTTACCACTG32310.21528417711271336No Hit
GAGTACATGGTAAGCAGTGGTATCA31910.2126189443412777No Hit
GCTTACCATGTACTCTGCGTTGATA31360.20895424928055376No Hit
GTACATGGGAAGCAGTGGTATCAAC30890.20582260077411685No Hit
AAGCAGTGGTATCAACGCAGAGTAC30470.20302410636410945No Hit
GCTTACCACTGCTTCCCATGTACTC29050.19356253002551294No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA28420.1893647884105018No Hit
GTACATAAGCAGTGGTATCAACGCA28150.18756575628978278No Hit
CTGCTTACCATGTACTCTGCGTTGA27080.18043625862619242No Hit
GCGTTGATACCACTGCTTACCATGT26960.17963668879476175No Hit
GTACATGATAAGCAGTGGTATCAAC25690.1711745747454536No Hit
GTATCAACGCAGAGTACATGGTAAG25350.1689091268897333No Hit
ATGGTAAGCAGTGGTATCAACGCAG24930.1661106324797259No Hit
ATCATGTACTCTGCGTTGATACCAC24780.16511117019043753No Hit
GTATCAACGCAGAGTACTTTTTTTT23800.15858134990042025No Hit
CAGTGGTATCAACGCAGAGTACATG23230.15478339320112447No Hit
TATCAACGCAGAGTACATGGTAAGC21780.14512192440467028No Hit
ACGCAGAGTACATGGGAAGCAGTGG20780.1384588424760812No Hit
ACGCAGAGTACATGGTAAGCAGTGG19970.13306174611392405No Hit
GAGTAAGCAGTGGTATCAACGCAGA19970.13306174611392405No Hit
GGTATCAACGCAGAGTACATGGTAA18370.12240081502818151No Hit
AAAAAGTACTCTGCGTTGATACCAC16810.11200640721958253No Hit
GCTTATGTACTCTGCGTTGATACCA16220.10807518888171497No Hit
GTGGTATCAACGCAGAGTACATGGT15530.1034776623509885No Hit
TACCACTGCTTACCATGTACTCTGC15350.10227830760384246No Hit
GTGATAAGCAGTGGTATCAACGCAG15330.10214504596527069No Hit
TATCAACGCAGAGTACTTTTTTTTT15240.10154536859169766No Hit
GGTATCAACGCAGAGTACTTTTTTT15220.10141210695312587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG307.7375135E-418.9945355
ACGGGTC500.001502758713.2961755
GTATTAT751.4546746E-512.6866961
ATTAGAC604.1018208E-412.6630233
TAGAACT1552.5465852E-1111.6418114
GTCGTGC600.00588955211.0797778
CTCCTAT803.715851E-410.70441
TCTAGAC803.7805462E-410.6844253
TCGGGGG803.7817104E-410.68407112
GGTATAA1105.9307004E-610.3800241
GTCTATA856.4975745E-410.0747291
TCTATAC856.5798056E-410.0609592
GTCCTAC1054.0333034E-59.9681191
TCTTAGG1054.0943865E-59.9544942
AGCCTCG1252.5618174E-69.8820982
GAACAAA4900.09.7092071
GTGTTAG800.0044568179.5150221
GTTCTAA1304.152007E-69.5150221
GGATATA800.0044568179.5150221
CTTCTAG800.0044568179.5150221
Produced by FastQC (version 0.11.5)