FastQCFastQC Report
Wed 25 May 2016
SRR1294501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500807
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC156741.0443714614870532No Hit
ACCATGTACTCTGCGTTGATACCAC106220.7077525624547326No Hit
GTACATGGTAAGCAGTGGTATCAAC90270.6014764056937367No Hit
GTGGTAAGCAGTGGTATCAACGCAG83710.5577665882421924No Hit
GTATCAACGCAGAGTACTTTTTTTT81120.5405092060471467No Hit
CATGTACTCTGCGTTGATACCACTG80670.5375108191792816No Hit
GGTAAGCAGTGGTATCAACGCAGAG73200.4877375971727211No Hit
GTATCAACGCAGAGTACATGGGAAG58570.39025670855746275No Hit
ACTCTGCGTTGATACCACTGCTTAC54980.3663362444338279No Hit
GGTATCAACGCAGAGTACTTTTTTT54300.36180534872238734No Hit
TATCAACGCAGAGTACTTTTTTTTT53570.35694129891451737No Hit
CCATGTACTCTGCGTTGATACCACT53440.35607509826380074No Hit
GGTATCAACGCAGAGTACATGGGAA50580.33701868394803597No Hit
GTGGTATCAACGCAGAGTACATGGG49070.32695743023586643No Hit
GTACTTTTTTTTTTTTTTTTTTTTT45820.3053024139679519No Hit
GTACTCTGCGTTGATACCACTGCTT44960.2995721635093653No Hit
GCAGTGGTATCAACGCAGAGTACAT39900.26585696895070454No Hit
TATCAACGCAGAGTACATGGGAAGC39390.26245879716712406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA38490.2564620234313939No Hit
AAGCAGTGGTATCAACGCAGAGTAC35210.2346071147056217No Hit
GTACATGGGAAGCAGTGGTAAGCAG34130.2274109862227455No Hit
GAGTACATGGTAAGCAGTGGTATCA34090.22714446294560195No Hit
GCTTACCATGTACTCTGCGTTGATA32840.21881561053486556No Hit
GCGTTGATACCACTGCTTACCACTG32520.21668342431771706No Hit
GTACATGGGAAGCAGTGGTATCAAC31070.20702195552126287No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29120.19402894576051416No Hit
ATGGTAAGCAGTGGTATCAACGCAG29080.1937624224833706No Hit
GCTTACCACTGCTTCCCATGTACTC28530.1900977274226466No Hit
GTACATAAGCAGTGGTATCAACGCA28110.18729923301263918No Hit
GCGTTGATACCACTGCTTACCATGT27670.18436747696406No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26880.17910364224047465No Hit
CTGCTTACCATGTACTCTGCGTTGA26420.17603862455332364No Hit
ATCATGTACTCTGCGTTGATACCAC26270.1750391622640353No Hit
GTACATGATAAGCAGTGGTATCAAC25740.17150772884188306No Hit
GTATCAACGCAGAGTACATGGTAAG25360.1689757577090192No Hit
CAGTGGTATCAACGCAGAGTACATG24950.16624389411829768No Hit
TATCAACGCAGAGTACATGGTAAGC23600.15724873351470242No Hit
ACGCAGAGTACATGGGAAGCAGTGG22920.15271783780326184No Hit
ACGCAGAGTACATGGTAAGCAGTGG22260.14832020373039304No Hit
GAGTAAGCAGTGGTATCAACGCAGA21160.14099081360894505No Hit
GGTATCAACGCAGAGTACATGGTAA19210.12799780384819634No Hit
GTGGTATCAACGCAGAGTACTTTTT17480.11647067211173721No Hit
GTGGTATCAACGCAGAGTACATGGT16830.11213966885815431No Hit
AAAAAGTACTCTGCGTTGATACCAC16610.11067379083386471No Hit
GTGATAAGCAGTGGTATCAACGCAG16600.11060716001457882No Hit
GCTTATGTACTCTGCGTTGATACCA16440.10954106690600457No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT16210.10800855806242908No Hit
TACCACTGCTTACCATGTACTCTGC15500.10327776989313084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC551.9572547E-413.8205335
GTCTATA655.4197597E-513.1622271
CAAGGGT550.00306445412.0929664
GTAAGGC550.00306445412.0929663
GGCGGTC550.003070579112.08974312
GTACTAA802.8475855E-511.88256551
CCTAGAC600.005868062411.0852193
CGAGGGT600.00588263611.08152614
GTCCAAG2050.010.6653271
ATCTTAG909.4524185E-510.5622811
GAATAAG1553.621608E-910.4259931
ACTGATC1106.040951E-610.36401758
ACATCGA750.00264228510.1357336
CACGGGT750.002643746610.13505654
TAGACAC750.002643746610.13505655
GTTCTAT856.5541716E-410.0652321
CTAGACT856.582356E-410.0605354
ATTAGGG856.582356E-410.0605353
GTATTAG1401.0487602E-69.5060531
GTATTAC800.0044916369.5060521
Produced by FastQC (version 0.11.5)