FastQCFastQC Report
Wed 25 May 2016
SRR1294500_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294500_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347645
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC121170.36195594216232607No Hit
ACCATGTACTCTGCGTTGATACCAC91980.2747603165807605No Hit
GTACATGGTAAGCAGTGGTATCAAC86790.25925688058321594No Hit
CATGTACTCTGCGTTGATACCACTG66680.19918480006093836No Hit
GTGGTAAGCAGTGGTATCAACGCAG58630.17513804480463133No Hit
GGTAAGCAGTGGTATCAACGCAGAG53540.15993332626368684No Hit
GTGGTATCAACGCAGAGTACATGGG52840.15784230406748623No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA50890.15201731366378454No Hit
CCATGTACTCTGCGTTGATACCACT43590.13021093933197816No Hit
GTATCAACGCAGAGTACATGGGAAG39770.1187999324898548No Hit
ACTCTGCGTTGATACCACTGCTTAC38570.11521532301065375No Hit
GTACTCTGCGTTGATACCACTGCTT37250.1112722525835326No Hit
GCAGTGGTATCAACGCAGAGTACAT35370.10565636439945096No Hit
GTACATGATAAGCAGTGGTATCAAC34860.10413290537079051No Hit
ATCATGTACTCTGCGTTGATACCAC33910.10129508953308967No Hit
GGTATCAACGCAGAGTACATGGGAA33740.10078726985686952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAG751.4616553E-512.6818671
ATAACGC701.0935747E-412.2119513
TGTACCG752.0794415E-411.3974815
CGTAGCG600.005886426711.0812153
CGGCTCG600.0058910111.0800568
ATACGTG700.001494934110.8547445
GTCTTAA2750.010.7219431
CCGTCCA856.6151324E-410.055859
TGAACCG1356.468872E-79.8496755
GCGTAGC1201.6992302E-59.5011652
AAGTGCG800.0045271069.4976177
ACCGACT800.00452877959.4971918
TACTCCG1356.8718655E-69.1461275
TCGACCG850.0074565588.9385349
ATAGGAC1502.7108726E-68.8649723
TTAGTAC2054.1600288E-98.8031963
GTTCTAG2751.8189894E-128.64672851
TATACTG3850.08.6344565
GTATTAG4650.08.5909411
TAGGACC2002.5491318E-88.5483674
Produced by FastQC (version 0.11.5)