FastQCFastQC Report
Wed 25 May 2016
SRR1294500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347645
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC127190.3799387330496513No Hit
GTATCAACGCAGAGTACTTTTTTTT102330.3056775733388696No Hit
ACCATGTACTCTGCGTTGATACCAC95570.28548427327270365No Hit
GTACATGGTAAGCAGTGGTATCAAC87240.26060110913791634No Hit
CATGTACTCTGCGTTGATACCACTG68610.20495004697332003No Hit
GGTATCAACGCAGAGTACTTTTTTT65530.19574954931003735No Hit
GTGGTAAGCAGTGGTATCAACGCAG62790.18756469099919496No Hit
TATCAACGCAGAGTACTTTTTTTTT62450.18654905164675464No Hit
GGTAAGCAGTGGTATCAACGCAGAG57640.17218074198429045No Hit
GTGGTATCAACGCAGAGTACATGGG54980.16423485763872814No Hit
CCATGTACTCTGCGTTGATACCACT45820.1368723386141601No Hit
ACTCTGCGTTGATACCACTGCTTAC42060.12564056224599682No Hit
GTATCAACGCAGAGTACATGGGAAG41240.1231910791018761No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38250.11425942714953347No Hit
GTACTCTGCGTTGATACCACTGCTT37240.11124238083787259No Hit
ATCATGTACTCTGCGTTGATACCAC36600.10933058911563204No Hit
GCAGTGGTATCAACGCAGAGTACAT35450.10589533836473101No Hit
GTACATGATAAGCAGTGGTATCAAC35070.1047602120296507No Hit
GGTATCAACGCAGAGTACATGGGAA33970.10147432000704973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAC400.005283376714.24894711
CCGCTCG600.005880640411.082689
CTAGGGT1900.011.0011594
AGACCGT1002.7544698E-49.5008586
GACCGTC1106.839845E-59.500157
ACCGTCT1002.758605E-49.4994398
GTGTTAG2451.8189894E-129.3112761
GCCTTAC1951.6680133E-99.2615361
GTATTAG4450.08.9712581
CTACCCT2356.548362E-118.8945544
GGTTTAG2054.105459E-98.8097541
TCCTATA2054.138201E-98.8058062
TTACACC2201.6043487E-98.6372744
TAGGACA2106.373739E-98.5961444
GTATTAT4250.08.4988211
CTAGACC1804.0309897E-78.4453343
CTAGGAC2606.002665E-118.4047323
CTACTAG2159.631549E-98.3999991
GTATTAA4550.08.3562741
TAAGACT3300.08.3493654
Produced by FastQC (version 0.11.5)