FastQCFastQC Report
Wed 25 May 2016
SRR1294499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668399
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC97420.3650878298185541No Hit
ACCATGTACTCTGCGTTGATACCAC64360.24119331479287767No Hit
GTACATGGTAAGCAGTGGTATCAAC54720.20506678349077478No Hit
CATGTACTCTGCGTTGATACCACTG49280.18468002723730598No Hit
GTGGTAAGCAGTGGTATCAACGCAG48140.18040780258124814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA42390.15885930102657062No Hit
GGTAAGCAGTGGTATCAACGCAGAG38000.14240748853526028No Hit
GTGGTATCAACGCAGAGTACATGGG37960.1422575859157495No Hit
GTATCAACGCAGAGTACATGGGAAG35520.13311352612559066No Hit
ACTCTGCGTTGATACCACTGCTTAC30660.11490035785502843No Hit
CCATGTACTCTGCGTTGATACCACT30450.11411336910259673No Hit
GGTATCAACGCAGAGTACATGGGAA30170.11306405076602112No Hit
GTACTCTGCGTTGATACCACTGCTT27130.1016714516832003No Hit
ATTCCATTCCATTCCATTCCATTCC26960.10103436555027939No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA350.002174670316.28131311
TACCGCA456.7746034E-414.7748917
CTCGTGG1700.013.4081411
GACTACG500.001502248713.2974017
TCGTGGG2000.012.82201412
TCTCGTG1750.011.93985310
AGTACCG658.041154E-411.6898055
TAGACGG600.00588361911.0817914
GTCTCGT1803.6379788E-1211.080969
GGATTAG1355.5784767E-810.5701341
CGTGGGC1951.6370905E-109.74150513
GTTCTAA2900.09.5131221
GTACTAG1502.597426E-79.5131211
ATTAGGC1106.847232E-59.4990353
GTATAGA3250.09.3667651
GTCTAAG1752.6122507E-89.2413181
TAGACAG2800.09.1587545
TATTCCG1151.11367495E-49.08501155
GTCCTAC2106.511982E-109.0601161
TAGACTG3150.09.0456825
Produced by FastQC (version 0.11.5)