FastQCFastQC Report
Wed 25 May 2016
SRR1294499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668399
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC101760.38135226403547595No Hit
GTATCAACGCAGAGTACTTTTTTTT85790.32150364319578895No Hit
ACCATGTACTCTGCGTTGATACCAC64810.2428797192623742No Hit
GTACATGGTAAGCAGTGGTATCAAC58490.21919510537966774No Hit
GTGGTAAGCAGTGGTATCAACGCAG54630.2047295025968755No Hit
GGTATCAACGCAGAGTACTTTTTTT53400.2001199970469184No Hit
TATCAACGCAGAGTACTTTTTTTTT51910.1945361244701411No Hit
CATGTACTCTGCGTTGATACCACTG51450.19281224434576688No Hit
GGTAAGCAGTGGTATCAACGCAGAG43760.16399346574481552No Hit
GTGGTATCAACGCAGAGTACATGGG38700.14503078437669928No Hit
GTATCAACGCAGAGTACATGGGAAG36430.13652381071946135No Hit
CCATGTACTCTGCGTTGATACCACT33570.12580577342443916No Hit
ACTCTGCGTTGATACCACTGCTTAC32510.12183335400740294No Hit
GGTATCAACGCAGAGTACATGGGAA32300.12104636525497123No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30490.11426327172210753No Hit
GTACTCTGCGTTGATACCACTGCTT26760.1002848524527254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC350.002171034516.2853937
CTCGGAA500.001502170213.29749412
ACCGTCT604.0983025E-412.6649928
GACCGGC550.003073770612.08863211
AGAGTCG600.005866103311.0862315
TATTCCG1252.2339373E-710.6427835
GGGAGTA1054.1080988E-59.9521847
TCTAACC1606.611481E-89.5026623
TAGACAG3900.09.5024845
TTAGGAC1651.0617623E-79.2147043
TAGGACC1554.245685E-79.1961254
CTACACT3250.09.0640784
CACCGCG1054.5034513E-49.04574517
CTAACCT1606.717164E-78.9087464
GTACTAA2054.0763553E-98.8127981
TTATACT5550.08.7321774
TAGGACT2659.094947E-128.6061854
ACCGCGC1000.0029107798.548388518
GTCTAAG2252.408342E-98.452041
GAACTGT4050.08.4466526
Produced by FastQC (version 0.11.5)