FastQCFastQC Report
Wed 25 May 2016
SRR1294498_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294498_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362059
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC145700.43336538710355765No Hit
ACCATGTACTCTGCGTTGATACCAC117570.3496964211514432No Hit
GTACATGGTAAGCAGTGGTATCAAC111830.33262355003288163No Hit
CATGTACTCTGCGTTGATACCACTG77100.22932375666221202No Hit
GTGGTAAGCAGTGGTATCAACGCAG77060.22920478195058444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA73770.2194191119192138No Hit
GGTAAGCAGTGGTATCAACGCAGAG65980.1962487868297374No Hit
CCATGTACTCTGCGTTGATACCACT56750.16879537212166712No Hit
GTGGTATCAACGCAGAGTACATGGG51840.15419122626937837No Hit
GTATCAACGCAGAGTACATGGGAAG51300.1525850676624057No Hit
ACTCTGCGTTGATACCACTGCTTAC48960.1456250470321907No Hit
GAGTACATGGTAAGCAGTGGTATCA42830.12739217247525994No Hit
GTACTCTGCGTTGATACCACTGCTT42630.1267972989171219No Hit
GGTATCAACGCAGAGTACATGGGAA41820.12438806100666289No Hit
GCAGTGGTATCAACGCAGAGTACAT40870.12156241160550722No Hit
GTACATGGGAAGCAGTGGTAAGCAG39710.11811214496830662No Hit
GCTTACCATGTACTCTGCGTTGATA39310.11692239785203057No Hit
GCGTTGATACCACTGCTTACCATGT37110.11037878871251218No Hit
GCTTACCACTGCTTCCCATGTACTC34300.1020208152206728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTA750.002652677710.13161212
GTACCGT750.002654642310.1307076
TGTATCG800.00452686759.497685
AATCCGA1002.764164E-49.4975386
GTCTTAG2850.09.3450941
GTATAGG2451.8189894E-129.3178481
GACAGTG5000.09.3077267
TAAGGCT2900.09.1701744
CTAGGAC2201.6916601E-109.0658323
CAATCCG1054.505895E-49.0454095
TACACCG950.00182866748.9978015
GTTCTAG2750.08.9931351
TAACCCG850.00745343238.93899255
GTCTAGG2451.6370905E-118.9296051
CTTAGAC2356.730261E-118.8913133
ATAATAC6900.08.6716653
GTATTAA7500.08.62418651
GTACATA10750.08.5828941
GTGCTAG4050.08.4550851
TTAGTAC3100.08.2720493
Produced by FastQC (version 0.11.5)