FastQCFastQC Report
Wed 25 May 2016
SRR1294498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362059
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC151810.4515387743046746No Hit
ACCATGTACTCTGCGTTGATACCAC123420.3670964727269807No Hit
GTACATGGTAAGCAGTGGTATCAAC116660.34698974646191516No Hit
GTATCAACGCAGAGTACTTTTTTTT106570.3169783754538513No Hit
GTGGTAAGCAGTGGTATCAACGCAG87200.25936487134818276No Hit
CATGTACTCTGCGTTGATACCACTG80090.23821711635637566No Hit
GGTAAGCAGTGGTATCAACGCAGAG75520.2246242555529216No Hit
GGTATCAACGCAGAGTACTTTTTTT66590.1980631511820584No Hit
TATCAACGCAGAGTACTTTTTTTTT63790.18973492136812592No Hit
CCATGTACTCTGCGTTGATACCACT60720.1806036122507071No Hit
GTATCAACGCAGAGTACATGGGAAG54930.16338202274261102No Hit
GTGGTATCAACGCAGAGTACATGGG54460.16198406988098663No Hit
ACTCTGCGTTGATACCACTGCTTAC51380.1528230170856609No Hit
GGTATCAACGCAGAGTACATGGGAA44860.133430139090361No Hit
GAGTACATGGTAAGCAGTGGTATCA43760.1301583345206018No Hit
GTACTCTGCGTTGATACCACTGCTT43520.12944448625083615No Hit
GCAGTGGTATCAACGCAGAGTACAT42930.12768960925432896No Hit
GTACATGGGAAGCAGTGGTAAGCAG41170.12245472194271428No Hit
GCTTACCATGTACTCTGCGTTGATA39510.11751727141016859No Hit
GCGTTGATACCACTGCTTACCATGT38320.11397777373924728No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37770.11234187145436769No Hit
GCTTACCACTGCTTCCCATGTACTC35060.10428133474159734No Hit
TATCAACGCAGAGTACATGGGAAGC34920.1038649232509007No Hit
CTGCTTACCATGTACTCTGCGTTGA33670.100146963512538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAC551.9560673E-413.8224683
TACGACC752.069215E-411.4035364
GAATCGT700.00148814410.8606726
GTCCTAG2650.010.4037871
GACTGCG1201.5249643E-610.2917967
GTCTAAG2053.6379788E-1210.2025361
GTATTGG4150.09.3923671
GTATTAT4550.09.1934941
CCTATAC2800.09.1635563
GTATTAG3750.09.1266321
GTGTTAC2401.0913936E-119.1107861
TTTGCGT850.0074441058.94035710
TCTAGAC2850.08.6693553
TTAGACT3400.08.6644524
TTAGTAC3000.08.5526513
CTTAGAC2803.6379788E-128.4847743
ACACCGT1356.7762805E-58.447196
CTAGGAC2606.002665E-118.4064523
TAATACT5000.08.3625944
CTTAGGC1951.5297883E-78.2846193
Produced by FastQC (version 0.11.5)