FastQCFastQC Report
Wed 25 May 2016
SRR1294497_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294497_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022848
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC102070.33766170181233063No Hit
ATTCCATTCCATTCCATTCCATTCC72690.2404685912093496No Hit
ACCATGTACTCTGCGTTGATACCAC64330.21281255293021678No Hit
GTACATGGTAAGCAGTGGTATCAAC60550.2003077892107046No Hit
GAATGGAATGGAATGGAATGGAATG60330.19957999872967477No Hit
GTGGTAAGCAGTGGTATCAACGCAG52990.1752982617716802No Hit
CATGTACTCTGCGTTGATACCACTG47830.15822826685298103No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA47750.1579636157689702No Hit
GTGGTATCAACGCAGAGTACATGGG47180.15607797679539295No Hit
GGTAAGCAGTGGTATCAACGCAGAG42850.14175373687330622No Hit
GTATCAACGCAGAGTACATGGGAAG38280.12663554369918698No Hit
GGTATCAACGCAGAGTACATGGGAA34290.11343607088414634No Hit
CCATGTACTCTGCGTTGATACCACT33220.10989636263550136No Hit
ACTCTGCGTTGATACCACTGCTTAC31960.10572810806233061No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG550.003075049312.0880459
AGTCCGC550.003075430212.0878456
CTTAGAC1406.8394E-1011.5331743
TAGACAG3000.010.7639395
CTAGTAC1303.8567305E-710.2285163
AATCGTT750.002654018310.1309347
GTTCTAG2350.010.1185521
ACGGGTC856.612171E-410.0562745
TATTCCG2107.2759576E-129.949865
CTGTGCG1554.0825398E-89.804139
TTAGACT2054.1836756E-119.7297254
GTTAGAC800.00452574959.4979083
ACACCGT1002.763797E-49.4975936
TTAGACA2750.09.3253724
GTCTAAG1451.6798585E-69.1834591
TAGGACT2700.09.1462844
CCAGTAC2600.09.1326043
GTACTAA2304.1836756E-119.0978991
GACAGTC2304.1836756E-119.0848057
TGGACCG1151.1142828E-49.0846555
Produced by FastQC (version 0.11.5)