FastQCFastQC Report
Wed 25 May 2016
SRR1294497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022848
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC107740.3564188473915989No Hit
GTATCAACGCAGAGTACTTTTTTTT103560.3425908282520325No Hit
ATTCCATTCCATTCCATTCCATTCC73430.24291661373644988No Hit
ACCATGTACTCTGCGTTGATACCAC68090.2252511538787263No Hit
GGTATCAACGCAGAGTACTTTTTTT63710.2107615070291328No Hit
TATCAACGCAGAGTACTTTTTTTTT62020.20517075287940378No Hit
GAATGGAATGGAATGGAATGGAATG61390.20308662559281843No Hit
GTACATGGTAAGCAGTGGTATCAAC60750.20096941692073172No Hit
GTGGTAAGCAGTGGTATCAACGCAG58400.1931952913279133No Hit
CATGTACTCTGCGTTGATACCACTG50030.16550617166327913No Hit
GTGGTATCAACGCAGAGTACATGGG48350.1599484988990515No Hit
GGTAAGCAGTGGTATCAACGCAGAG47680.1577320460704607No Hit
GTATCAACGCAGAGTACATGGGAAG41270.13652687796409216No Hit
GGTATCAACGCAGAGTACATGGGAA35760.11829903455284553No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34870.11535479124322494No Hit
CCATGTACTCTGCGTTGATACCACT34320.11353531504065041No Hit
ACTCTGCGTTGATACCACTGCTTAC32540.10764682842140921No Hit
GTACATGGGAAGCAGTGGTAAGCAG30430.1006666560806233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGCG400.00528703914.247395518
CCGGTAC500.001497512213.303073
TATACCG658.0128555E-411.6948145
TCGACCG658.0329E-411.6913319
TTACGCC600.005867748511.0858914
CTTACGC600.005867748511.0858913
GTCTTAG2300.010.7462331
ACCGACC803.775811E-410.6866068
ATACCGT1002.3947618E-510.4522396
TTCGTTG750.002651738710.13198412
TAGGACC1803.929017E-1010.0300924
TCGTGCT951.6463787E-49.9991649
GTCTTAA2850.09.6730591
TAAACGT800.00450894059.5021934
ATCGACC900.00111500679.4992068
TAGACTG3050.09.3462645
GTATTAG2850.09.3395061
GTATTAA4800.09.3082351
TATTCCG3200.09.2050975
TAGCCTG2501.8189894E-129.1219545
Produced by FastQC (version 0.11.5)