FastQCFastQC Report
Wed 25 May 2016
SRR1294496_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294496_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3678419
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC107830.29314224399123645No Hit
ACCATGTACTCTGCGTTGATACCAC83330.2265375423517549No Hit
GTACATGGTAAGCAGTGGTATCAAC79260.2154730062018492No Hit
GTGGTAAGCAGTGGTATCAACGCAG55840.15180434855300606No Hit
CATGTACTCTGCGTTGATACCACTG51670.14046795647804122No Hit
GGTAAGCAGTGGTATCAACGCAGAG46700.12695671700260355No Hit
GTGGTATCAACGCAGAGTACATGGG43840.11918163754591306No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA41170.11192308434683487No Hit
ACTCTGCGTTGATACCACTGCTTAC39360.10700249210326503No Hit
CCATGTACTCTGCGTTGATACCACT39270.10675782177071182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC604.1016124E-412.664024
TAAAGCG550.003075565412.0878895
TAACCCG1104.9933624E-711.2240110
TAGACAG5450.011.1531775
ACCGTTC600.00589037511.0802658
CTAGACA4750.010.1978684
CGAACAT1252.577206E-69.87860815
CTAGTAC1951.6370905E-109.7415543
GTATTAA7400.09.5147031
GTGTAGG4000.09.5147031
CGCAATC800.00452318379.49866115
TACATCG900.00111682339.4976275
GAACCGA900.00111696229.49749856
ATCGTCC800.00452825949.497378
GTACTAG2550.09.328141
TGACGAA1451.7140846E-69.16999912
AGAACCG1054.5063975E-49.045365
TTGACGG850.0074515528.9393084
CTGCGCA1606.772061E-78.9040269
GTTCTAA4500.08.8803891
Produced by FastQC (version 0.11.5)