FastQCFastQC Report
Wed 25 May 2016
SRR1294495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2340059
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC315401.3478292641339384No Hit
ACCATGTACTCTGCGTTGATACCAC170320.7278448962184287No Hit
CATGTACTCTGCGTTGATACCACTG162180.6930594485010848No Hit
GTACATGGTAAGCAGTGGTATCAAC155350.6638721502321095No Hit
GTGGTAAGCAGTGGTATCAACGCAG148960.636565146434342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA133570.5707975739073246No Hit
GGTAAGCAGTGGTATCAACGCAGAG118970.5084059846354301No Hit
GTATCAACGCAGAGTACATGGGAAG103600.4427238800389221No Hit
GGTATCAACGCAGAGTACATGGGAA93540.39973351099267157No Hit
ACTCTGCGTTGATACCACTGCTTAC90510.3867851195204907No Hit
GTACTCTGCGTTGATACCACTGCTT90510.3867851195204907No Hit
GTGGTATCAACGCAGAGTACATGGG83150.3553329210930152No Hit
CCATGTACTCTGCGTTGATACCACT82800.3538372323090999No Hit
GTACATGGGAAGCAGTGGTAAGCAG77350.3305472212452763No Hit
GCAGTGGTATCAACGCAGAGTACAT75150.3211457488892374No Hit
GTACATGGGAAGCAGTGGTATCAAC73020.31204341428998156No Hit
TATCAACGCAGAGTACATGGGAAGC67870.29003542218379963No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA63080.2695658528267877No Hit
GCTTACCACTGCTTCCCATGTACTC62790.26832656783440073No Hit
AAGCAGTGGTATCAACGCAGAGTAC60630.25909603133938075No Hit
GCGTTGATACCACTGCTTACCACTG57940.2476005946858605No Hit
GCTTACCATGTACTCTGCGTTGATA56370.2408913621408691No Hit
GAGTACATGGTAAGCAGTGGTATCA54060.23101981616702824No Hit
ATCATGTACTCTGCGTTGATACCAC53470.22849851221699966No Hit
GCGTTGATACCACTGCTTACCATGT51110.2184132964168852No Hit
GTACATGATAAGCAGTGGTATCAAC48640.2078580069989688No Hit
CTGCTTACCATGTACTCTGCGTTGA47230.201832517898053No Hit
CAGTGGTATCAACGCAGAGTACATG46270.19773005723359968No Hit
ACGCAGAGTACATGGGAAGCAGTGG43670.1866192262673719No Hit
GTGATAAGCAGTGGTATCAACGCAG43070.18405518835208856No Hit
GTACATAAGCAGTGGTATCAACGCA41740.17837157097321052No Hit
GTATCAACGCAGAGTACATGGTAAG41240.1762348727104744No Hit
TATCAACGCAGAGTACATGGTAAGC37130.15867121299078357No Hit
ACGCAGAGTACATGGTAAGCAGTGG36900.15768833178992495No Hit
ATGGTAAGCAGTGGTATCAACGCAG36390.15550889956193412No Hit
GTATCAACGCAGAGTACTTTTTTTT36010.15388500888225468No Hit
AAAAAGTACTCTGCGTTGATACCAC31330.1338855131430447No Hit
GGTATCAACGCAGAGTACATGGTAA31210.133372705559988No Hit
GAGTACATGGGAAGCAGTGGTAAGC31190.13328723762947858No Hit
GAGTACATGGGAAGCAGTGGTATCA30720.13127874126250663No Hit
GTGGTATCAACGCAGAGTACATGGT27970.11952690081745802No Hit
GATAAGCAGTGGTATCAACGCAGAG26790.1144842929174008No Hit
TACCACTGCTTACCATGTACTCTGC26170.11183478707160802No Hit
GCTTCCCATGTACTCTGCGTTGATA25830.11038183225294745No Hit
ACTCTGCGTTGATACCACTGCTTAT25390.10850153778173968No Hit
AAAGTACTCTGCGTTGATACCACTG24920.10649304141476774No Hit
TATCAACGCAGAGTACTTTTTTTTT24220.1035016638469372No Hit
GCGTTGATACCACTGCTTCCCATGT24090.1029461222986258No Hit
GCTTATGTACTCTGCGTTGATACCA24070.10286065436811635No Hit
GGTATCAACGCAGAGTACTTTTTTT23930.10226237885455024No Hit
CTTCCCATGTACTCTGCGTTGATAC23810.10174957127149359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC500.00148483713.3178751
TATGCGT1001.448534E-712.34677710
ATAACGC550.003073279312.0887873
ACTCCGC1052.7311762E-711.7588358
TCCGCGT907.4866766E-611.60808210
CTCCGCG907.4866766E-611.6080829
GTCCTAC1001.8998144E-611.4153211
GTCGCCC752.0797033E-411.39702611
CTATGCG1104.9884875E-711.2243439
ATGCGTT1104.9884875E-711.22434311
GAACAAA8500.010.9676621
TTAGGAC1355.705806E-810.5537033
GTCTTAT2350.010.5247651
CCGCGTT1002.4067174E-510.44727211
CTGTCGC1303.8572762E-710.22819
CTATACC1409.7221346E-810.1765684
GCGTTAA750.002619077710.1469541
TCTGTCG1151.0300391E-59.9104578
CGTTAAT900.0011120439.5017852
TAGACTG2002.6557245E-109.4983325
Produced by FastQC (version 0.11.5)