FastQCFastQC Report
Wed 25 May 2016
SRR1294495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2340059
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC330111.4106909270236347No Hit
ACCATGTACTCTGCGTTGATACCAC178770.7639550968586689No Hit
CATGTACTCTGCGTTGATACCACTG171420.7325456323964481No Hit
GTGGTAAGCAGTGGTATCAACGCAG163810.7000250848376045No Hit
GTACATGGTAAGCAGTGGTATCAAC163270.6977174507138495No Hit
GGTAAGCAGTGGTATCAACGCAGAG133620.5710112437335981No Hit
GTATCAACGCAGAGTACTTTTTTTT119620.5111836923769871No Hit
GTATCAACGCAGAGTACATGGGAAG105500.4508433334373193No Hit
GGTATCAACGCAGAGTACATGGGAA98000.41879285949627765No Hit
ACTCTGCGTTGATACCACTGCTTAC95200.40682734922495545No Hit
GTACTCTGCGTTGATACCACTGCTT90320.38597317418065097No Hit
CCATGTACTCTGCGTTGATACCACT89180.38110150214161265No Hit
GTGGTATCAACGCAGAGTACATGGG86040.3676830370516299No Hit
GCAGTGGTATCAACGCAGAGTACAT80740.34503403546662714No Hit
TATCAACGCAGAGTACTTTTTTTTT79360.3391367482614755No Hit
GTACATGGGAAGCAGTGGTAAGCAG78810.3367863801724657No Hit
GTACTTTTTTTTTTTTTTTTTTTTT77830.33259845157750295No Hit
GGTATCAACGCAGAGTACTTTTTTT77440.3309318269325688No Hit
GTACATGGGAAGCAGTGGTATCAAC75110.3209748130282185No Hit
TATCAACGCAGAGTACATGGGAAGC72660.31050499154081157No Hit
AAGCAGTGGTATCAACGCAGAGTAC71830.3069580724246696No Hit
GCTTACCACTGCTTCCCATGTACTC64030.2736255795259863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA60920.2603353163317677No Hit
GCGTTGATACCACTGCTTACCACTG59900.25597645187578605No Hit
GCTTACCATGTACTCTGCGTTGATA57980.24777153054687936No Hit
GAGTACATGGTAAGCAGTGGTATCA56010.2393529393916991No Hit
ATCATGTACTCTGCGTTGATACCAC55580.23751537888574603No Hit
GCGTTGATACCACTGCTTACCATGT53760.2297377972093866No Hit
CAGTGGTATCAACGCAGAGTACATG51530.22020812295758357No Hit
GTGATAAGCAGTGGTATCAACGCAG49840.2129860828295355No Hit
CTGCTTACCATGTACTCTGCGTTGA49420.21119125628883714No Hit
ACGCAGAGTACATGGGAAGCAGTGG48590.2076443371726952No Hit
GTACATGATAAGCAGTGGTATCAAC48490.207216997520148No Hit
GTATCAACGCAGAGTACATGGTAAG44080.1883713188428155No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43830.18730296971144744No Hit
GTACATAAGCAGTGGTATCAACGCA41480.1772604878765877No Hit
GAGTACTTTTTTTTTTTTTTTTTTT41220.17614940477996494No Hit
ACGCAGAGTACATGGTAAGCAGTGG40030.171064062914653No Hit
ATGGTAAGCAGTGGTATCAACGCAG39130.167218006041728No Hit
TATCAACGCAGAGTACATGGTAAGC38110.16285914158574635No Hit
GAGTACATGGGAAGCAGTGGTAAGC33520.14324425153382886No Hit
GGTATCAACGCAGAGTACATGGTAA33510.1432015175685741No Hit
AAAAAGTACTCTGCGTTGATACCAC31610.1350820641701769No Hit
GAGTACATGGGAAGCAGTGGTATCA31550.13482566037864857No Hit
GATAAGCAGTGGTATCAACGCAGAG30970.13234709039387468No Hit
GTGGTATCAACGCAGAGTACTTTTT29890.1277318221463647No Hit
GTGGTATCAACGCAGAGTACATGGT28710.12268921424630747No Hit
TACCACTGCTTACCATGTACTCTGC27370.11696286290217468No Hit
GCTTCCCATGTACTCTGCGTTGATA26910.11499710050045747No Hit
AAAGTACTCTGCGTTGATACCACTG26580.11358687964705165No Hit
ACTCTGCGTTGATACCACTGCTTAT26240.11213392482839109No Hit
GTAAGCAGTGGTATCAACGCAGAGT25370.10841606985123026No Hit
CTTCCCATGTACTCTGCGTTGATAC24410.10431360918677691No Hit
GCTTATGTACTCTGCGTTGATACCA24150.10320252609015415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCG400.00528059414.2498247
TAGGGTG1750.011.9462835
CAACGAT658.035553E-411.69066311
CACAACG803.7756242E-410.6864549
GTGTAAC909.45221E-510.5628751
CTGCGGT1002.403521E-510.44853111
CGGTGAT1002.4046547E-510.44808514
GGGCTGT1201.5184469E-610.294846
GTTATAC1606.146365E-910.0966453
GCGGTAT1054.0754618E-59.9592821
GCGTTAT800.0044911389.5065871
GACCTGT1201.6948088E-59.502936
GCTATCC800.0045062159.5027263
TACACGA800.0045062159.5027262
GACTGTC1002.756516E-49.4998837
GCGGTGA1201.7046988E-59.49825913
CGATGAT1106.852382E-59.49825914
CTTACAC2054.2018655E-109.2709523
GTACATA15600.09.2628281
CTAGACA1856.666596E-99.2458954
Produced by FastQC (version 0.11.5)