FastQCFastQC Report
Wed 25 May 2016
SRR1294494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872050
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG43660.15201685207430232No Hit
CCCATGTACTCTGCGTTGATACCAC40300.1403178914016121No Hit
GTATCAACGCAGAGTACTTTTTTTT39270.13673160286206718No Hit
ATTCCATTCCATTCCATTCCATTCC34670.12071516860778886No Hit
ACCATGTACTCTGCGTTGATACCAC34580.12040180358977037No Hit
GTACATGGTAAGCAGTGGTATCAAC34090.11869570515833638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG550.003073920712.0886045
ACCGATC550.003075123812.0879728
TCCGGAT803.758798E-410.69179318
CCTCTCG800.00452452769.49818913
TCAGGAC3150.09.3475873
GTCTTAC2650.09.3336061
CTAGACT2357.2759576E-129.2964244
GTATAGG4100.09.2810711
CTTAGAC1951.6898412E-99.2548073
TATTCCG1554.27528E-79.1917965
TAGGACT3450.09.0855414
GTATTAG4450.08.9786541
ACACCGT850.00745066868.9393166
TAGACTG4600.08.8787425
GTCTAAT3150.08.7580911
CCGGATG1000.00289495458.55358419
AAACCCG1000.00291097568.548375
CTGTCGC2356.202754E-108.48739
CTAGCTC1252.7495352E-48.3585513
GTCTTAT3650.08.3402511
Produced by FastQC (version 0.11.5)