FastQCFastQC Report
Wed 25 May 2016
SRR1294494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872050
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT145010.5049006806984558No Hit
GGTATCAACGCAGAGTACTTTTTTT88720.3089082710955589No Hit
TATCAACGCAGAGTACTTTTTTTTT81410.2834560679653906No Hit
GTGGTATCAACGCAGAGTACATGGG44930.15643878066189656No Hit
CCCATGTACTCTGCGTTGATACCAC42510.14801274351073274No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42110.14662001009731726No Hit
ACCATGTACTCTGCGTTGATACCAC37130.12928047910029422No Hit
ATTCCATTCCATTCCATTCCATTCC34890.12148117198516738No Hit
GTACATGGTAAGCAGTGGTATCAAC34890.12148117198516738No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33980.11831270346964712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGC600.005870422311.0851826
TTAGACA4400.010.149424
GTTCTAG3150.09.9606331
GTATTAG4250.09.843451
GCTATAC1606.625851E-89.501423
TCAGGAC2850.09.0013453
GTCTATA3400.08.948591
TTATACT5650.08.9129924
TAGGACC2252.6193447E-108.8681464
TAATACT5150.08.8558464
GTCCTAT2151.02591E-98.8445371
TATACTG6350.08.8282445
GCCTAGT1201.7487702E-48.7155541
CCACGTA1201.7610379E-48.7099372
CCTATAC1901.0094482E-78.501273
AGACAGT5050.08.4667596
ATAATAC6650.08.4298313
ATTAGAC3850.08.3908633
CCTAGAC2752.1827873E-118.2921493
CTAGGAC3100.08.275433
Produced by FastQC (version 0.11.5)