FastQCFastQC Report
Wed 25 May 2016
SRR1294493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141457
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC37520.17520781411907874No Hit
ATTCCATTCCATTCCATTCCATTCC30280.1413990568103866No Hit
ACCATGTACTCTGCGTTGATACCAC27920.1303785226600394No Hit
GTACATGGTAAGCAGTGGTATCAAC27070.1264092624787703No Hit
GTGGTATCAACGCAGAGTACATGGG25510.11912450261667641No Hit
GAATGGAATGGAATGGAATGGAATG24840.11599579165026427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGCT701.3187673E-917.6376976
TAGTACG1600.016.6247962
GTACGGT702.51257E-816.281334
AGTACGG1650.016.1172493
CTCGTGG1055.456968E-1215.37681211
TACGGGC902.2992026E-914.7734555
GTACGGG1058.913048E-1114.4722944
ACCGTCC604.1040697E-412.6626668
CCGTCCT550.003075828812.0873739
GCTCGTG752.0806087E-411.39639910
TCGTGGG1604.5474735E-1111.27900412
CGTGGGG1900.010.99780913
TAACACG951.3648152E-510.9970394
TAGTACT1900.010.9970394
GTATTAA4350.010.7152841
CACCGTC803.782953E-410.6841257
GTCTAAG1252.2007043E-710.6540541
GTCATAG1701.364242E-1010.6316721
GTCCTAT1553.5852281E-910.4331181
GTGTAGG2800.010.1920161
Produced by FastQC (version 0.11.5)