FastQCFastQC Report
Wed 25 May 2016
SRR1294493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141457
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52450.24492670177360554No Hit
CCCATGTACTCTGCGTTGATACCAC41040.19164522098739317No Hit
GGTATCAACGCAGAGTACTTTTTTT34950.16320663921806508No Hit
ATTCCATTCCATTCCATTCCATTCC31190.14564850006327468No Hit
TATCAACGCAGAGTACTTTTTTTTT30510.14247309191825938No Hit
ACCATGTACTCTGCGTTGATACCAC29880.13953116966625995No Hit
GTACATGGTAAGCAGTGGTATCAAC28080.13112567751769005No Hit
GTGGTATCAACGCAGAGTACATGGG26360.12309376279794552No Hit
GAATGGAATGGAATGGAATGGAATG24470.11426799604194714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCG402.758467E-416.6275755
TGTGCGA400.00528272114.24887910
CTAGGAC2150.010.6062943
CCGTGCA909.5210926E-510.5552189
TTAGACA3300.010.3652424
TAGACAG3600.010.2932615
TAGGACT2400.010.2932614
GTGTAAC1803.8926373E-1010.0337841
CTTAGAC2105.456968E-129.9539223
TAACACT3000.09.8181884
GTCTTAG2054.0017767E-119.7375351
GTTCTAG2401.8189894E-129.505691
TAAGACT2303.6379788E-129.50147154
CGAATGT800.00452216959.49858815
TATACTG3850.09.3780765
GTATTAG3700.09.2487791
AGGACTG3600.09.2375425
GTATTAA4650.09.1990561
GTGCTAG2501.8189894E-129.1254631
ACTGCGG1054.4946498E-49.047338
Produced by FastQC (version 0.11.5)