FastQCFastQC Report
Wed 25 May 2016
SRR1294492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186452
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC214510.6731938846089632No Hit
ACCATGTACTCTGCGTTGATACCAC128430.4030501636302697No Hit
GTACATGGTAAGCAGTGGTATCAAC121720.3819922597296303No Hit
CATGTACTCTGCGTTGATACCACTG104360.32751160224600906No Hit
GTGGTAAGCAGTGGTATCAACGCAG91280.28646281193000867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA87140.2734703049033847No Hit
GGTAAGCAGTGGTATCAACGCAGAG81540.25589589926350687No Hit
GTATCAACGCAGAGTACATGGGAAG80790.2535421842224518No Hit
GTGGTATCAACGCAGAGTACATGGG67700.2124620110392374No Hit
GGTATCAACGCAGAGTACATGGGAA66620.20907266138011807No Hit
ACTCTGCGTTGATACCACTGCTTAC64900.20367480821929848No Hit
CCATGTACTCTGCGTTGATACCACT63580.19953226974704155No Hit
GTACTCTGCGTTGATACCACTGCTT60620.19024294105167755No Hit
GTACATGGGAAGCAGTGGTAAGCAG55530.17426906163971714No Hit
GCAGTGGTATCAACGCAGAGTACAT53010.16636057910177213No Hit
TATCAACGCAGAGTACATGGGAAGC50690.15907975390810847No Hit
GCTTACCACTGCTTCCCATGTACTC48170.15117127137016345No Hit
ATTCCATTCCATTCCATTCCATTCC47610.14941383080617565No Hit
GTGATAAGCAGTGGTATCAACGCAG45370.1423840685502245No Hit
GAGTACATGGTAAGCAGTGGTATCA43930.1378649356713988No Hit
GTACATGGGAAGCAGTGGTATCAAC42360.1329378255187902No Hit
GCTTACCATGTACTCTGCGTTGATA41510.13027028180559444No Hit
GCGTTGATACCACTGCTTACCATGT40700.12772826956125496No Hit
GAATGGAATGGAATGGAATGGAATG39680.12452721710542007No Hit
GCGTTGATACCACTGCTTACCACTG39640.1244016856365638No Hit
AAGCAGTGGTATCAACGCAGAGTAC39260.12320913668242923No Hit
GTACATGATAAGCAGTGGTATCAAC37420.11743468911504079No Hit
CTGCTTACCATGTACTCTGCGTTGA36030.11307247057228541No Hit
ATCATGTACTCTGCGTTGATACCAC35330.11087566986730069No Hit
GTATCAACGCAGAGTACATGGTAAG33300.10450494782284496No Hit
GTATCAACGCAGAGTACTTTTTTTT32410.10171187264079296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT657.9411414E-411.7073891
CACCGTT600.00588822711.0807337
GCGAAAA1451.41662895E-810.496281
GTCTTAG2251.8189894E-129.7236371
CTAGACT2350.09.7001554
CGCGCCC800.00449659689.50538118
TGTACTG4100.09.2662645
CGAATAG1651.0718395E-79.20923710
GTGTAGG2700.09.1599471
CTTGGAC3050.09.0309513
AGCAACG850.0074526638.9390787
TGTGGCG850.0074545828.93879812
CCAGTAC2900.08.843033
CTCGATC1401.0780908E-58.82642518
TAGACTG3100.08.5787665
GTTCTAA3000.08.56102851
GTCTAAG2002.4854671E-88.56102851
CTGTTCG1000.00291397268.5474579
GTATTAC2605.820766E-118.4146851
GTCTAGA2605.820766E-118.4146851
Produced by FastQC (version 0.11.5)