FastQCFastQC Report
Wed 25 May 2016
SRR1294492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186452
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC228110.7158745840200951No Hit
ACCATGTACTCTGCGTTGATACCAC137050.4301021951687959No Hit
GTACATGGTAAGCAGTGGTATCAAC126360.3965539101169577No Hit
GTATCAACGCAGAGTACTTTTTTTT114190.35836096071743745No Hit
CATGTACTCTGCGTTGATACCACTG109800.3445838820104618No Hit
GTGGTAAGCAGTGGTATCAACGCAG105370.33068127183462986No Hit
GGTAAGCAGTGGTATCAACGCAGAG90030.28253995352825023No Hit
GTATCAACGCAGAGTACATGGGAAG82480.25884588878162923No Hit
GGTATCAACGCAGAGTACTTTTTTT70840.22231623134445458No Hit
TATCAACGCAGAGTACTTTTTTTTT70670.22178272260181542No Hit
GGTATCAACGCAGAGTACATGGGAA69910.21939762469354632No Hit
GTGGTATCAACGCAGAGTACATGGG68100.21371732572780006No Hit
CCATGTACTCTGCGTTGATACCACT67630.2122423309687389No Hit
ACTCTGCGTTGATACCACTGCTTAC67060.21045350753753703No Hit
GTACTCTGCGTTGATACCACTGCTT58840.1846567906875735No Hit
GCAGTGGTATCAACGCAGAGTACAT56810.1782860686431178No Hit
GTACATGGGAAGCAGTGGTAAGCAG56540.17743873122833798No Hit
TATCAACGCAGAGTACATGGGAAGC54110.16981269449531958No Hit
GTACTTTTTTTTTTTTTTTTTTTTT53240.16708238504769568No Hit
GTGATAAGCAGTGGTATCAACGCAG49550.15550210704570475No Hit
ATTCCATTCCATTCCATTCCATTCC47350.14859787625860987No Hit
AAGCAGTGGTATCAACGCAGAGTAC47180.14806436751597074No Hit
GCTTACCACTGCTTCCCATGTACTC46880.14712288149954872No Hit
GAGTACATGGTAAGCAGTGGTATCA46770.14677766996019398No Hit
GTACATGGGAAGCAGTGGTATCAAC44320.13908886749274743No Hit
GCTTACCATGTACTCTGCGTTGATA43590.13679791818612047No Hit
GCGTTGATACCACTGCTTACCACTG42020.13187080803351187No Hit
GCGTTGATACCACTGCTTACCATGT41930.13158836222858528No Hit
GAATGGAATGGAATGGAATGGAATG39910.1252490230513436No Hit
GTACATGATAAGCAGTGGTATCAAC37020.1161793744264781No Hit
CTGCTTACCATGTACTCTGCGTTGA36720.11523788841005607No Hit
CAGTGGTATCAACGCAGAGTACATG36350.11407672232313557No Hit
ATCATGTACTCTGCGTTGATACCAC36210.11363736218213863No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35350.11093843560172884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA34530.10836504049017527No Hit
GTATCAACGCAGAGTACATGGTAAG33260.10437941635398869No Hit
ACGCAGAGTACATGGGAAGCAGTGG32060.1006134722883006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG658.0079195E-411.6957085
GTACGGA1701.382432E-1010.6245721
GTCTTAC2700.010.2103591
GTGCGGG750.002634588910.1399421
ACCGGCC750.0026511110.1323048
TGCGTGA750.002651800410.13198610
CTAGGAC2550.010.0617493
GTGTAGG2600.09.8718191
TACGGAA1901.0477379E-99.5030612
CTTAGAC2054.2200554E-109.2709883
CTAATAC2600.09.1372723
GTACTAG1651.0409822E-68.6419961
GTAGGAC1107.1268703E-48.6388763
GTACTAA3900.08.5312011
TCCGTAC1451.6840191E-58.5197183
CGTACCA1804.0192936E-78.44695
TAGGACT2502.3464963E-108.3624324
GTCCTAG2053.8220605E-88.3469041
GTCTAAC1502.5664447E-58.2387031
ACTGTCC2201.46810635E-88.20371158
Produced by FastQC (version 0.11.5)