##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1411188_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	54185479
Sequences flagged as poor quality	0
Sequence length	151
%GC	23
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.765549677986606	34.0	31.0	34.0	31.0	34.0
2	32.961901028871594	34.0	31.0	34.0	31.0	34.0
3	33.04414690142354	34.0	33.0	34.0	31.0	34.0
4	36.40981844969941	37.0	37.0	37.0	35.0	37.0
5	36.304881110306326	37.0	37.0	37.0	35.0	37.0
6	36.18568006015043	37.0	37.0	37.0	35.0	37.0
7	36.24584034774335	37.0	37.0	37.0	35.0	37.0
8	36.10743308184099	37.0	37.0	37.0	35.0	37.0
9	37.94653300010506	39.0	39.0	39.0	35.0	39.0
10-14	38.41396264486284	39.4	39.2	39.4	36.6	39.4
15-19	39.61507452577839	41.0	39.6	41.0	37.0	41.0
20-24	38.97406144181174	40.6	38.0	41.0	35.2	41.0
25-29	38.37917015368638	40.0	35.8	41.0	35.0	41.0
30-34	38.17903096510414	40.0	35.0	41.0	35.0	41.0
35-39	38.041234571350756	40.0	35.0	41.0	35.0	41.0
40-44	37.85685573804746	40.0	35.0	41.0	34.6	41.0
45-49	37.651880740963826	40.0	35.0	41.0	34.0	41.0
50-54	37.38836678734537	39.0	35.0	41.0	33.8	41.0
55-59	36.90919112480301	38.4	35.0	40.8	33.0	41.0
60-64	36.32146383351156	36.8	35.0	40.0	33.0	41.0
65-69	35.667230879328386	35.6	35.0	39.2	32.4	41.0
70-74	35.017558217027116	35.0	35.0	38.0	31.8	40.2
75-79	34.38987552366198	35.0	34.6	36.4	31.0	39.0
80-84	33.82029277991619	35.0	34.0	35.6	31.0	37.6
85-89	33.342362355050874	35.0	34.0	35.0	30.0	36.4
90-94	32.94070355269905	35.0	34.0	35.0	29.6	36.0
95-99	32.531143157376164	35.0	33.6	35.0	29.0	35.2
100-104	31.621579475194817	34.2	32.2	35.0	26.8	35.0
105-109	31.735733209998937	35.0	33.0	35.0	26.0	35.0
110-114	31.33229795015746	35.0	33.0	35.0	24.4	35.0
115-119	30.849964928795778	34.8	32.4	35.0	20.8	35.0
120-124	30.405721521812143	34.0	32.0	35.0	17.2	35.0
125-129	29.983144805271543	34.0	31.2	35.0	8.2	35.0
130-134	29.55743083862007	34.0	31.0	35.0	2.6	35.0
135-139	29.07230057706051	34.0	30.2	35.0	2.0	35.0
140-144	28.49259291036257	34.0	29.2	35.0	2.0	35.0
145-149	27.739795150652817	33.0	29.0	35.0	2.0	35.0
150-151	25.975164480875033	32.0	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17547809307890105
1101	2	0.16496073678571577
1101	3	0.16578465935579345
1101	4	0.1045021771355863
1101	5	0.09085267727427038
1101	6	0.06992672638657638
1101	7	0.029203366405475606
1101	8	0.015777722074687972
1101	9	-0.03910328159542331
1101	10-14	0.09073450884585554
1101	15-19	0.20971886544525375
1101	20-24	0.1775319792732617
1101	25-29	0.17870537978467382
1101	30-34	0.1897986914757297
1101	35-39	0.20909103495733916
1101	40-44	0.20861449564196732
1101	45-49	0.22134560878279785
1101	50-54	0.22497656691683687
1101	55-59	0.21875081761488957
1101	60-64	0.20697013525990116
1101	65-69	0.20941108963358346
1101	70-74	0.20701950805305813
1101	75-79	0.20199179714263238
1101	80-84	0.22294302184405268
1101	85-89	0.23080571877027722
1101	90-94	0.24550093705106946
1101	95-99	0.26821808671039093
1101	100-104	0.2593530067052434
1101	105-109	0.28038629069222765
1101	110-114	0.2552272099009869
1101	115-119	0.26751362274133683
1101	120-124	0.24918740266742034
1101	125-129	0.2488862764710582
1101	130-134	0.22177536506200113
1101	135-139	0.2985750905755893
1101	140-144	0.3222326598283658
1101	145-149	0.35282862764252343
1101	150-151	0.41544392310332157
1102	1	0.15518490301378307
1102	2	0.15771151206451606
1102	3	0.15609691554269034
1102	4	0.10665754787907389
1102	5	0.1057753473340739
1102	6	-0.0401222828765313
1102	7	0.005356265545586325
1102	8	-0.08686309390708402
1102	9	-0.075156972508438
1102	10-14	0.08056233892018838
1102	15-19	0.20138595348213784
1102	20-24	0.16775985095326718
1102	25-29	0.13535681225633311
1102	30-34	0.13747042727616332
1102	35-39	0.14700971391197726
1102	40-44	0.1684649245695553
1102	45-49	0.17186008830401533
1102	50-54	0.17232271268773047
1102	55-59	0.16066449271006888
1102	60-64	0.14260961968363262
1102	65-69	0.14992632913819648
1102	70-74	0.16211686192684738
1102	75-79	0.15238259926930198
1102	80-84	0.1636916781050175
1102	85-89	0.1708744175236916
1102	90-94	0.1833143956260912
1102	95-99	0.18933307096894225
1102	100-104	0.16721192751058922
1102	105-109	0.126202021834942
1102	110-114	0.13728978153646665
1102	115-119	0.11760418760732705
1102	120-124	0.12546494102755545
1102	125-129	0.10065702881369631
1102	130-134	0.0825237687741307
1102	135-139	0.08540680058205652
1102	140-144	0.05426483813538496
1102	145-149	0.07742682856320116
1102	150-151	-0.018772973163375895
1103	1	0.1281680424258056
1103	2	0.14205468343366334
1103	3	0.1319966733490716
1103	4	0.09051977142708267
1103	5	0.06909021301488139
1103	6	-6.895175343188953E-4
1103	7	0.04826696820212817
1103	8	0.04567322819801234
1103	9	0.01771057251070829
1103	10-14	0.0921713791823322
1103	15-19	0.17549516688283973
1103	20-24	0.1426894897779576
1103	25-29	0.102735239238541
1103	30-34	0.13018139839593346
1103	35-39	0.12914576365906782
1103	40-44	0.14548495614146617
1103	45-49	0.13199191734602067
1103	50-54	0.12619323169967345
1103	55-59	0.12909878717766787
1103	60-64	0.10620973402572531
1103	65-69	0.1294262272988007
1103	70-74	0.12922799119133543
1103	75-79	0.11677904467516242
1103	80-84	0.12910181185236524
1103	85-89	0.14425076185425922
1103	90-94	0.15181441503469273
1103	95-99	0.13539617834732098
1103	100-104	0.10430294335487389
1103	105-109	0.15134178271894427
1103	110-114	0.12475368829003841
1103	115-119	0.1053319997260651
1103	120-124	0.09922040121134756
1103	125-129	0.03964766213629645
1103	130-134	0.028464586056536234
1103	135-139	0.008489256975884985
1103	140-144	-0.03453480930081554
1103	145-149	-0.08765598491583049
1103	150-151	-0.22907204240278034
1104	1	0.13755600349693964
1104	2	0.12827844075643924
1104	3	0.11883068530018193
1104	4	0.08272526929390267
1104	5	0.0836306850832571
1104	6	0.041179364954381015
1104	7	0.049614570471788966
1104	8	-0.003167005638609055
1104	9	0.03612879653888257
1104	10-14	0.07861283922704132
1104	15-19	0.1451063417301981
1104	20-24	0.12682513438360843
1104	25-29	0.10662812359451834
1104	30-34	0.11967477472991561
1104	35-39	0.14469794689589577
1104	40-44	0.1464079345076641
1104	45-49	0.15195061397189136
1104	50-54	0.14624841154877544
1104	55-59	0.15707621689632845
1104	60-64	0.1444695822736506
1104	65-69	0.1481689733928846
1104	70-74	0.15769248291557858
1104	75-79	0.16477974435605347
1104	80-84	0.17402369172778265
1104	85-89	0.18637990146279293
1104	90-94	0.20571196610656983
1104	95-99	0.2160056311311962
1104	100-104	0.1709893637374833
1104	105-109	0.1629520040595196
1104	110-114	0.15046208991071097
1104	115-119	0.15551340559292726
1104	120-124	0.12440334178246815
1104	125-129	0.12882032646406216
1104	130-134	0.09342915028514653
1104	135-139	0.09218578684649259
1104	140-144	0.05617844328336474
1104	145-149	0.08446785887842267
1104	150-151	0.010693214975805887
1105	1	0.1496931016380998
1105	2	0.1416973467364997
1105	3	0.123602804006822
1105	4	0.09299822421235149
1105	5	0.10394745380962434
1105	6	0.016832463192734792
1105	7	0.04425050115204243
1105	8	-0.048384638830306415
1105	9	-0.029887975069833317
1105	10-14	0.09445697427067756
1105	15-19	0.16986484056843665
1105	20-24	0.1421794092101223
1105	25-29	0.1300590688786727
1105	30-34	0.14725932435273137
1105	35-39	0.15001057548739283
1105	40-44	0.15878662811275746
1105	45-49	0.14872129404049161
1105	50-54	0.15862434997365682
1105	55-59	0.15483260579676994
1105	60-64	0.12905582906017798
1105	65-69	0.15187827366727902
1105	70-74	0.15864376684356785
1105	75-79	0.15175889521712094
1105	80-84	0.1870330591129914
1105	85-89	0.1939300284569896
1105	90-94	0.2127006866923864
1105	95-99	0.2172934490422378
1105	100-104	0.20213413201632235
1105	105-109	0.2194706880025734
1105	110-114	0.20657623225626764
1105	115-119	0.2147822194664073
1105	120-124	0.21335213790534624
1105	125-129	0.19029905340842745
1105	130-134	0.1924779571267372
1105	135-139	0.21605637593116
1105	140-144	0.14235273101664347
1105	145-149	0.09449324083245969
1105	150-151	0.0974155577857303
1106	1	0.08098296508771341
1106	2	0.09032421456117135
1106	3	0.08251167273149917
1106	4	0.06454434690582644
1106	5	0.07351715961992511
1106	6	0.00423237759607531
1106	7	0.04548710676637313
1106	8	0.11455040109952819
1106	9	0.03602217655478057
1106	10-14	0.09834818449532179
1106	15-19	0.13167087326719695
1106	20-24	0.11060589681464705
1106	25-29	0.08924714589577576
1106	30-34	0.09107555214502838
1106	35-39	0.12673031139571833
1106	40-44	0.14335390327884312
1106	45-49	0.1304603907506987
1106	50-54	0.11363311707376766
1106	55-59	0.13390806028699132
1106	60-64	0.11272524502092551
1106	65-69	0.14003604518663337
1106	70-74	0.13887602288440348
1106	75-79	0.11856512668105523
1106	80-84	0.14991705113888543
1106	85-89	0.15078092443859958
1106	90-94	0.1604357092142621
1106	95-99	0.15018651043953213
1106	100-104	0.14799407915824148
1106	105-109	0.1665116574685861
1106	110-114	0.19219501945738315
1106	115-119	0.16840031144674938
1106	120-124	0.14558728463894255
1106	125-129	0.1539255854326207
1106	130-134	0.1593524485019664
1106	135-139	0.14921569997571638
1106	140-144	0.12978839278767396
1106	145-149	0.1279148443606566
1106	150-151	0.12398801426220274
1107	1	0.11942928146898169
1107	2	0.10442075835143072
1107	3	0.09099226458106813
1107	4	0.07515429186108236
1107	5	0.08297856446377949
1107	6	0.05911944470162922
1107	7	0.05801870050206048
1107	8	0.07573107414870606
1107	9	0.09814951923564053
1107	10-14	0.09504849740840626
1107	15-19	0.11729474354656588
1107	20-24	0.09810887549375735
1107	25-29	0.12021757590534321
1107	30-34	0.14119144044365584
1107	35-39	0.1299377301485407
1107	40-44	0.14701908823294474
1107	45-49	0.13294474926989608
1107	50-54	0.10755542807159202
1107	55-59	0.11357413409373152
1107	60-64	0.09646754563428317
1107	65-69	0.08926692892438126
1107	70-74	0.10952256852485931
1107	75-79	0.10585859118985042
1107	80-84	0.14682341819219857
1107	85-89	0.13541463634815187
1107	90-94	0.11843251712669911
1107	95-99	0.12070900555443842
1107	100-104	0.12410997600920837
1107	105-109	0.14904610057224943
1107	110-114	0.1556000900900969
1107	115-119	0.15601902896533204
1107	120-124	0.13146538224023274
1107	125-129	0.11767103262701539
1107	130-134	0.12924326080607784
1107	135-139	0.15231915515928307
1107	140-144	0.14697810031817582
1107	145-149	0.11161269796803808
1107	150-151	0.1418955273585567
1108	1	0.06379339032010023
1108	2	0.06736433378541307
1108	3	0.07393736465464684
1108	4	0.06343117287698874
1108	5	0.05001592495056428
1108	6	0.029601997810267733
1108	7	0.025059509476356823
1108	8	0.10114633854885113
1108	9	0.09007687065410863
1108	10-14	0.07472432252355077
1108	15-19	0.09078252862194347
1108	20-24	0.09242553540769904
1108	25-29	0.09083616524654303
1108	30-34	0.11474241534511975
1108	35-39	0.11342179160114796
1108	40-44	0.13223854610163954
1108	45-49	0.11707115405067725
1108	50-54	0.12233021376954412
1108	55-59	0.11469668145695522
1108	60-64	0.12304473027083418
1108	65-69	0.11195615274925075
1108	70-74	0.12386731804546969
1108	75-79	0.1081202839854214
1108	80-84	0.10866076446886552
1108	85-89	0.11324115689233594
1108	90-94	0.12069632667965635
1108	95-99	0.14027214829788193
1108	100-104	0.13410502354459553
1108	105-109	0.1371028020398697
1108	110-114	0.16482745564807644
1108	115-119	0.1300705581871462
1108	120-124	0.1395305436659413
1108	125-129	0.16240906000539468
1108	130-134	0.15334389032320317
1108	135-139	0.15508020212484652
1108	140-144	0.143958994497126
1108	145-149	0.11538738033907237
1108	150-151	0.1488359042122802
1109	1	0.06670024914998862
1109	2	0.04482343963144331
1109	3	0.03510100532715654
1109	4	0.05027641964665719
1109	5	0.06785619706475643
1109	6	0.032133882971379535
1109	7	0.051305467736192156
1109	8	-0.025416951677776467
1109	9	-0.0013880388111502384
1109	10-14	0.037985551348100444
1109	15-19	0.06278667402673221
1109	20-24	0.05527958729949489
1109	25-29	0.061285755483204696
1109	30-34	0.07169476013720555
1109	35-39	0.06333836084401412
1109	40-44	0.07309513677152069
1109	45-49	0.06135416518385739
1109	50-54	0.03392384901268031
1109	55-59	0.058060062389415634
1109	60-64	0.05808237692165363
1109	65-69	0.052057658528717354
1109	70-74	0.07863701087602948
1109	75-79	0.061026992097716004
1109	80-84	0.07802422326103908
1109	85-89	0.06698596055657902
1109	90-94	0.07236272439722313
1109	95-99	0.07002268091713404
1109	100-104	0.04350114386480186
1109	105-109	0.07751128887991499
1109	110-114	0.1027662275384813
1109	115-119	0.07923541920529331
1109	120-124	0.08885916110315506
1109	125-129	0.08552619050110621
1109	130-134	0.031988980010300594
1109	135-139	0.06706215047443465
1109	140-144	0.07131654699096401
1109	145-149	0.10553657708593178
1109	150-151	0.15381961615388562
1110	1	-0.003207408614009921
1110	2	0.0032737264872864102
1110	3	0.008131187020858022
1110	4	0.01049927052512345
1110	5	0.006961462617965708
1110	6	0.026487772753085892
1110	7	0.02163877945564252
1110	8	0.11499219049146348
1110	9	0.0817576814372174
1110	10-14	0.030845437828872946
1110	15-19	0.014797249386809597
1110	20-24	0.016155202980350225
1110	25-29	0.007946652772446328
1110	30-34	0.006654335810011958
1110	35-39	0.005034070377213595
1110	40-44	0.012286865338865027
1110	45-49	0.011384193054126968
1110	50-54	-0.02328585557291518
1110	55-59	-0.022412776587088956
1110	60-64	-0.029845057145358567
1110	65-69	-0.033340362996575834
1110	70-74	-0.0034239381195249052
1110	75-79	-0.022630534900294208
1110	80-84	-0.007378438888977712
1110	85-89	-0.021177502227374134
1110	90-94	-0.03852365050779838
1110	95-99	-0.031065497832997835
1110	100-104	-0.056180290206263805
1110	105-109	-0.03274905711290188
1110	110-114	-0.04904547283555516
1110	115-119	-0.08826655443380105
1110	120-124	-0.06736495044718538
1110	125-129	-0.08826284894271907
1110	130-134	-0.11068782849114456
1110	135-139	-0.0800729519158132
1110	140-144	-0.13648798629801462
1110	145-149	-0.14173543923818954
1110	150-151	-0.08439800896055516
1111	1	-0.05820630577193242
1111	2	-0.060261355019846974
1111	3	-0.07430146596493614
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2216	35-39	-0.3389993661362496
2216	40-44	-0.38000778929897905
2216	45-49	-0.40416968406283615
2216	50-54	-0.3415873367902691
2216	55-59	-0.34454885280989345
2216	60-64	-0.3706754591479182
2216	65-69	-0.37971021839385344
2216	70-74	-0.4078527462710895
2216	75-79	-0.41562336658216026
2216	80-84	-0.45907068509500704
2216	85-89	-0.5044963209450941
2216	90-94	-0.5606847348127673
2216	95-99	-0.5496249887919902
2216	100-104	-0.5828526781176535
2216	105-109	-0.5314250434600396
2216	110-114	-0.4814838051400123
2216	115-119	-0.4239627224680014
2216	120-124	-0.4530612548812982
2216	125-129	-0.41077964147092416
2216	130-134	-0.38499750041119185
2216	135-139	-0.3169959746824276
2216	140-144	-0.3621200286647479
2216	145-149	-0.3015827638884403
2216	150-151	0.016330295177162668
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	927.0
3	2704.0
4	11736.0
5	28952.0
6	37535.0
7	39995.0
8	47796.0
9	57653.0
10	68020.0
11	77393.0
12	86144.0
13	93757.0
14	102996.0
15	112751.0
16	124945.0
17	139320.0
18	159570.0
19	183832.0
20	217397.0
21	267320.0
22	341638.0
23	480152.0
24	550781.0
25	656864.0
26	758121.0
27	795813.0
28	881085.0
29	956411.0
30	1057877.0
31	1288801.0
32	1681662.0
33	2408190.0
34	4023095.0
35	1.2287093E7
36	1.485953E7
37	9147412.0
38	150206.0
39	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.99958992794522	23.20620238320191	34.04668361562255	23.747524073230327
2	14.127304022552813	25.67518721206927	38.43257277955355	21.76493598582436
3	11.808670489305511	21.68411283837532	43.239582241211664	23.267634431107506
4	12.170550342463523	20.43184484906002	46.497418616526396	20.90018619195006
5	10.056373756259713	19.95952880573153	49.33273935220063	20.651358085808123
6	9.296509130439565	16.482433444278914	46.80989380037222	27.411163624909303
7	12.137418073528853	18.251812782753344	44.006704157892635	25.60406498582517
8	5.8361185939651445	18.498866516490345	47.7286754163458	27.936339473198707
9	8.815113107317645	16.685040605556008	47.44564047295213	27.05420581417422
10-14	11.758740424750108	30.97528151767481	45.65379497947705	11.612183078098038
15-19	11.79041228865019	29.81240927521338	46.09526933399353	12.301909102142904
20-24	10.97421402891532	29.42673383215224	47.3642367644453	12.234815374487141
25-29	11.459747528134297	29.460380598712852	46.93755927839167	12.14231259476118
30-34	11.406737996514742	29.56488356638428	47.287552862239515	11.740825574861462
35-39	11.429096592327253	29.38604806146031	47.4367546226388	11.748100723573634
40-44	11.646946368385388	29.886279053842625	47.128829362151706	11.337945215620286
45-49	11.754650398760901	29.61900045579224	47.1094056879465	11.516943457500362
50-54	11.477798377515498	29.46036686891601	47.45504112317974	11.606793630388758
55-59	11.583021870052136	29.419824547997152	47.499291409392455	11.497862172558255
60-64	11.495706448416401	29.249379947432086	47.63677524536944	11.618138358782067
65-69	11.49174432332586	29.116479689814383	47.91246865461675	11.479307332243007
70-74	11.533258707632724	29.1643335061265	48.04537168672255	11.257036099518231
75-79	11.66919144606524	28.94117018214089	48.10951144738825	11.280126924405623
80-84	11.559633616114077	28.853973539834072	48.37196624875431	11.214426595297542
85-89	11.70675040653735	29.166078666436928	47.86854507947436	11.258625847551366
90-94	11.726935277020726	29.448559550346182	47.64134636207277	11.183158810560322
95-99	11.605469868725784	29.775731058816113	47.596871107400105	11.021927965058
100-104	11.628916232469223	30.212741283576865	47.420307191752684	10.738035292201232
105-109	11.71886670417429	30.573610035762712	47.08948299755727	10.618040262505726
110-114	11.649577257595563	30.4351907899473	47.144714554174946	10.770517398282188
115-119	11.88615940834846	30.101000605228766	47.083950771458866	10.928889214963908
120-124	12.070155349519716	29.70434878771918	47.07761872776965	11.147877134991452
125-129	12.250375370442724	29.57818282642316	47.03263378954988	11.138808013584237
130-134	12.425161797604563	29.728738489843355	46.71794504831677	11.128154664235309
135-139	12.603650176518416	29.699294295830157	46.4297366429041	11.267318884747324
140-144	12.62065547682863	29.56954187188509	46.37392120354477	11.435881447741517
145-149	12.887207819444965	29.59475564957942	45.94215882167846	11.575877709297162
150-151	12.756116669259306	29.563615211348488	45.77541007207973	11.904858047312475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	745635.0
1	1952904.5
2	3425326.5
3	2641078.5
4	964974.5
5	228220.5
6	96667.0
7	72428.0
8	75004.5
9	94089.5
10	131512.0
11	192336.0
12	283550.5
13	404114.0
14	553644.5
15	720854.5
16	889081.0
17	1053172.5
18	1198595.5
19	1314375.5
20	1420153.0
21	1530761.0
22	1657488.0
23	1793942.5
24	1895777.5
25	1947302.5
26	1965375.5
27	1965319.0
28	1946237.0
29	1897320.0
30	1801393.0
31	1656109.5
32	1496757.0
33	1371892.0
34	1274365.0
35	1160093.0
36	1026794.0
37	900108.5
38	790771.0
39	692748.0
40	605556.0
41	531191.0
42	469032.0
43	419901.5
44	388638.0
45	376962.0
46	375012.5
47	377196.0
48	384298.5
49	400024.0
50	422867.0
51	441509.5
52	443995.5
53	421244.5
54	371296.0
55	299481.0
56	218495.0
57	143969.5
58	87373.5
59	50768.5
60	29946.0
61	18648.0
62	12229.0
63	8632.5
64	6556.0
65	5246.0
66	4311.5
67	3449.5
68	2748.5
69	2208.5
70	1722.5
71	1352.5
72	1076.5
73	796.5
74	592.5
75	459.0
76	326.5
77	259.5
78	176.5
79	110.5
80	84.0
81	64.5
82	44.5
83	35.0
84	27.5
85	18.0
86	16.5
87	16.0
88	12.5
89	9.0
90	5.5
91	6.0
92	6.0
93	2.5
94	2.0
95	2.5
96	1.5
97	1.5
98	2.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008779104822530037
2	2.528352660682394E-4
3	8.858461876843425E-5
4	0.0
5	0.016007978816612475
6	6.551570763082117E-4
7	1.845512891009047E-6
8	0.007077541937019695
9	6.828397696733473E-5
10-14	3.691025782018094E-7
15-19	7.160590017115101E-5
20-24	1.2844769721422967E-4
25-29	9.4859362597865E-5
30-34	6.378092551327266E-4
35-39	8.77356828385701E-4
40-44	0.0011674714548523231
45-49	0.004537747096413044
50-54	0.005573448930847321
55-59	0.00604626933352384
60-64	0.008617806995855844
65-69	0.009544254467142388
70-74	0.007979628638144918
75-79	0.010755649128800724
80-84	0.00998533204809355
85-89	0.01121776555670939
90-94	0.011703135447044771
95-99	0.011504189157393995
100-104	0.008556535967874345
105-109	0.003701729756785946
110-114	0.004407822988886007
115-119	0.005521405467320867
120-124	0.004135794388751274
125-129	0.004067141309205738
130-134	0.005026808012530443
135-139	0.006533853839328429
140-144	0.0021001936699682954
145-149	0.0019001400725829148
150-151	1.2180385080659709E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	5.4185479E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.9885924380613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.05648118579985	22.016311872127403
2	20.6301984589296	16.499451961809118
3	11.42692983811553	13.708405203441712
4	6.078940650032337	9.72353120437226
5	3.1462793447331654	6.290764120641256
6	1.544319412701089	3.7053095753214027
7	0.7999645368491107	2.2392619080273093
8	0.3997446077761563	1.2788179359738707
9	0.22214851342822725	0.7995065727782311
>10	0.6234807144502892	4.186412950810193
>50	0.03963365456319541	1.110710947770451
>100	0.027528561375844973	2.1118613551039367
>500	0.0017579588978871305	0.4833825675911401
>1k	0.0014611614891862272	1.2914880351977052
>5k	2.7595197002207354E-4	0.7898301748630919
>10k+	8.554488887318438E-4	13.764953614170794
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	128520	0.2371853167524827	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92745	0.17116209307663405	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85301	0.15742409511596273	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84902	0.1566877354724501	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84795	0.15649026559311213	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82331	0.15194292182966584	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82237	0.151769443617911	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81735	0.15084299614662444	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80363	0.14831095246016002	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77769	0.14352369202088258	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76901	0.14192178683148673	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75758	0.13981236559706336	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74645	0.1377583097493703	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73787	0.13617485968888454	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71691	0.13230666466932958	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70688	0.13045561523964752	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70400	0.1299241075270369	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69813	0.1288407914600146	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69729	0.12868576837716983	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69183	0.1276781183386789	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68976	0.12729609717024	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68667	0.12672583368691823	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68382	0.12619986251298065	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67282	0.1241697983328707	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67080	0.12379700472888687	No Hit
CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66856	0.12338360984130083	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66468	0.12266755083958933	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66219	0.12220801812972808	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64833	0.11965013726278953	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64505	0.11904480903453857	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64300	0.11866647889188173	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64297	0.11866094235320869	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64245	0.11856497568287622	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63927	0.11797810258353533	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63855	0.1178452256553827	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62928	0.1161344352054173	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62326	0.11502343644502985	No Hit
GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62248	0.11487948643953115	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61736	0.11393458383933451	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61714	0.11389398255573233	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61332	0.11318899663136688	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60910	0.11241019019136103	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60770	0.11215181838661978	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59776	0.11031737857295679	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59559	0.10991690227560781	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58332	0.10765245795833972	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58048	0.10712833229729316	No Hit
TTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57862	0.10678506689956546	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57693	0.10647317522098496	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57384	0.10590291173766314	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56796	0.10481775015774983	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56449	0.10417735718456969	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56250	0.10381010011925888	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54465	0.10051585960880775	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.246019583955325E-4	0.0	0.0	1.845512891009047E-6	1.845512891009047E-6
2	0.0020319096930009606	5.536538673027141E-6	0.0	7.382051564036188E-6	1.845512891009047E-6
3	0.0033090046135792212	5.536538673027141E-6	0.0	2.2146154692108564E-5	1.845512891009047E-6
4	0.005390743154637426	5.536538673027141E-6	0.0	2.5837180474126658E-5	1.845512891009047E-6
5	0.00905962278196341	5.536538673027141E-6	0.0	2.5837180474126658E-5	1.845512891009047E-6
6	0.016670517944484722	5.536538673027141E-6	0.0	2.5837180474126658E-5	1.845512891009047E-6
7	0.025381338790047423	5.536538673027141E-6	0.0	3.321923203816284E-5	1.845512891009047E-6
8	0.05042125769525817	5.536538673027141E-6	0.0	4.798333516623522E-5	1.845512891009047E-6
9	0.14257878942068594	5.536538673027141E-6	0.0	4.798333516623522E-5	1.845512891009047E-6
10-11	0.3294351241224609	5.536538673027141E-6	0.0	4.798333516623522E-5	1.845512891009047E-6
12-13	0.5325984107291919	5.536538673027141E-6	0.0	5.5365386730271406E-5	1.845512891009047E-6
14-15	0.74254211169749	5.536538673027141E-6	0.0	6.920673341283926E-5	1.845512891009047E-6
16-17	0.9603126328365577	5.536538673027141E-6	0.0	1.024259654510021E-4	1.845512891009047E-6
18-19	1.188838803104426	5.536538673027141E-6	0.0	1.3472244104366043E-4	1.845512891009047E-6
20-21	1.48407841886938	5.536538673027141E-6	0.0	1.5502308284475995E-4	1.845512891009047E-6
22-23	1.8198833307351587	5.536538673027141E-6	0.0	1.5594583929026446E-4	1.845512891009047E-6
24-25	2.166084939472437	7.382051564036188E-6	0.0	1.56868595735769E-4	1.845512891009047E-6
26-27	2.4864779731853988	7.382051564036188E-6	0.0	1.5871410862677802E-4	1.845512891009047E-6
28-29	2.7941148217957066	7.382051564036188E-6	0.0	1.614823779632916E-4	1.845512891009047E-6
30-31	3.1232066805204397	8.304808009540712E-6	0.0	1.734782117548504E-4	1.845512891009047E-6
32-33	3.505102354082724	9.227564455045235E-6	0.0	1.7624648109136398E-4	1.845512891009047E-6
34-35	3.8907960931747048	9.227564455045235E-6	0.0	1.8639680199191374E-4	1.845512891009047E-6
36-37	4.164901818068269	9.227564455045235E-6	0.0	1.937788535559499E-4	1.845512891009047E-6
38-39	4.3592693902364505	9.227564455045235E-6	0.0	2.131567389115449E-4	1.845512891009047E-6
40-41	4.535348852411178	9.227564455045235E-6	0.0	2.3714840649466252E-4	1.845512891009047E-6
42-43	4.688511658261801	9.227564455045235E-6	0.0	3.0081860123447465E-4	3.691025782018094E-6
44-45	4.820111491493874	9.227564455045235E-6	0.0	3.4049712839116916E-4	3.691025782018094E-6
46-47	4.941101471115536	9.227564455045235E-6	0.0	3.727936039838275E-4	5.536538673027141E-6
48-49	5.06009183751979	9.227564455045235E-6	0.0	4.290817471596034E-4	7.382051564036188E-6
50-51	5.18461689708418	9.227564455045235E-6	0.0	5.056705321364788E-4	7.382051564036188E-6
52-53	5.31794966692091	9.227564455045235E-6	0.0	5.942551509049132E-4	7.382051564036188E-6
54-55	5.442986856312555	9.227564455045235E-6	0.0	7.049859243654559E-4	7.382051564036188E-6
56-57	5.565930311329351	9.227564455045235E-6	0.0	7.43741695076646E-4	7.382051564036188E-6
58-59	5.68883593333188	9.227564455045235E-6	0.0	7.714243884417816E-4	7.382051564036188E-6
60-61	5.813032491601671	9.227564455045235E-6	0.0	8.092574027074671E-4	7.382051564036188E-6
62-63	5.937416369429899	9.227564455045235E-6	0.0	8.600090072102158E-4	7.382051564036188E-6
64-65	6.0640296268304645	9.227564455045235E-6	0.0	9.329067664050733E-4	7.382051564036188E-6
66-67	6.190468483262832	9.227564455045235E-6	0.0	0.0010214913851735075	7.382051564036188E-6
68-69	6.315215927130588	9.227564455045235E-6	0.0	0.0010879298492498333	7.382051564036188E-6
70-71	6.44290973232884	9.227564455045235E-6	0.0	0.0011497545310986362	7.382051564036188E-6
72-73	6.573137426726449	9.227564455045235E-6	0.0	0.001200506135601385	7.382051564036188E-6
74-75	6.708515024846417	9.227564455045235E-6	0.0	0.0012521804965496383	7.382051564036188E-6
76-77	6.850865893425063	9.227564455045235E-6	0.0	0.0013490699233276132	7.382051564036188E-6
78-79	6.9918640010546	1.660961601908142E-5	0.0	0.0015963686507228256	7.382051564036188E-6
80-81	7.137581085146447	2.399166758311761E-5	0.0	0.0018676590457011553	7.382051564036188E-6
82-83	7.289974312121519	2.399166758311761E-5	0.0	0.001971007767597662	7.382051564036188E-6
84-85	7.4475792675008	2.399166758311761E-5	0.0	0.001996844948071789	7.382051564036188E-6
86-87	7.610141270505332	2.399166758311761E-5	0.0	0.002007918025417843	7.382051564036188E-6
88-89	7.780116698793048	2.399166758311761E-5	0.0	0.002015300076981879	7.382051564036188E-6
90-91	7.955415509014879	2.399166758311761E-5	0.0	0.0020226821285459153	7.382051564036188E-6
92-93	8.138294763436528	2.399166758311761E-5	0.0	0.002094657131295268	7.382051564036188E-6
94-95	8.33137139933745	2.399166758311761E-5	0.0	0.0021417177100159987	1.0150320900549757E-5
96-97	8.529083963620586	2.399166758311761E-5	0.0	0.0021694004033811346	1.2918590237063329E-5
98-99	8.732945776856564	2.399166758311761E-5	0.0	0.002224765790111406	1.2918590237063329E-5
100-101	8.945064414766916	2.399166758311761E-5	0.0	0.0022533712399220463	1.2918590237063329E-5
102-103	9.165849581213447	2.399166758311761E-5	0.0	0.002275517394614155	1.2918590237063329E-5
104-105	9.393899609155435	2.399166758311761E-5	0.0	0.002283822202623695	1.2918590237063329E-5
106-107	9.630792412114692	2.399166758311761E-5	0.0	0.002290281497742227	1.2918590237063329E-5
108-109	9.874869796758649	2.399166758311761E-5	0.0	0.002300431818642777	1.2918590237063329E-5
110-111	10.128178436883431	2.399166758311761E-5	1.845512891009047E-6	0.0023041228444247953	1.2918590237063329E-5
112-113	10.392051715552796	2.399166758311761E-5	1.845512891009047E-6	0.002318886947552868	1.2918590237063329E-5
114-115	10.664046173699045	2.399166758311761E-5	1.845512891009047E-6	0.002334573807126444	1.2918590237063329E-5
116-117	10.94656374634983	2.399166758311761E-5	1.845512891009047E-6	0.002342878615135985	1.2918590237063329E-5
118-119	11.238583864876418	2.399166758311761E-5	1.845512891009047E-6	0.0023631792569370844	1.2918590237063329E-5
120-121	11.539658069646297	2.399166758311761E-5	1.845512891009047E-6	0.00237240682139213	1.2918590237063329E-5
122-123	11.847903014754193	2.399166758311761E-5	1.845512891009047E-6	0.0023788661165106617	1.2918590237063329E-5
124-125	12.169230800746451	2.399166758311761E-5	3.691025782018094E-6	0.0023807116294016703	1.2918590237063329E-5
126-127	12.498390020691705	2.399166758311761E-5	3.691025782018094E-6	0.0023927074631932294	1.2918590237063329E-5
128-129	12.836975197727789	2.5837180474126658E-5	3.691025782018094E-6	0.0023963984889752475	1.2918590237063329E-5
130-131	13.186264349531726	2.5837180474126658E-5	3.691025782018094E-6	0.0024176218872218515	1.2918590237063329E-5
132-133	13.544639884054545	2.5837180474126658E-5	3.691025782018094E-6	0.0024305404774589146	1.2918590237063329E-5
134-135	13.91438377798598	2.5837180474126658E-5	3.691025782018094E-6	0.0024360770161319417	1.2918590237063329E-5
136-137	14.293886744085071	2.5837180474126658E-5	5.536538673027141E-6	0.002445304580586987	1.2918590237063329E-5
138-139	14.683962653536753	2.675993691963118E-5	5.536538673027141E-6	0.0024554549014875367	1.2918590237063329E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTTCT	11755	0.0	77.04239	1
GCCTTTT	14650	0.0	71.617775	1
GCTTTTC	13470	0.0	70.40931	1
GCCCTAC	17455	0.0	65.788185	7
GATTTCT	16905	0.0	60.906425	1
TGCTCTA	9405	0.0	56.501522	6
GGCTCTA	3665	0.0	53.605618	6
TGCCCTA	10475	0.0	53.49836	6
GACTTTT	17205	0.0	53.18567	1
GCTGCGT	1580	0.0	50.4796	5
GCCGCGT	1045	0.0	48.569324	5
GTTCTAC	7205	0.0	48.498203	7
GCTCTAC	33320	0.0	46.800232	7
GGCCCTA	3185	0.0	46.661575	6
CCGGAAG	3680	0.0	44.91585	7
CGCCCTA	9980	0.0	44.747128	6
CGCTTTT	10855	0.0	42.883987	1
CCGGCGT	1015	0.0	42.146965	5
CTTTTCT	99370	0.0	42.10205	2
CTGCGTG	1810	0.0	41.25472	6
>>END_MODULE