FastQCFastQC Report
Thu 26 May 2016
SRR1248497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106210222
Sequences flagged as poor quality0
Sequence length100
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3453920.32519657100424854No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2594140.24424579396887053No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2465840.23216597739528308No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2424110.22823697703974294No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2362730.22245787227523167No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2193700.20654320824223493No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2067570.1946677034532514No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2066400.19455754456477833No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1977760.18621183185174023No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1911530.17997608554099434No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1874180.17645947487050728No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1857590.17489747832369656No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1764280.1661120715857274No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1757710.16549348705814776No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720220.16196369498220237No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1642770.15467155317686843No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1634660.15390797318924726No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1632200.1536763570647654No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1513930.14254089403937034No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1494750.14073504149158073No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1437860.13537868323069696No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1432690.1348919127577005No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1431350.13476574787688517No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1402870.13208427339507867No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1401710.1319750560355669No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1396440.13147887027295735No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310590.12339584414012429No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1292340.12167755378573637No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1275530.12009484360177686No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1217720.11465186467645272No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1172670.11041027670575813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATCC10650.047.6951141
CCCAGGT9700.047.4850127
GGCCAGC2000.047.0326841
CCCTGTG19200.046.510887
GAGTACC4250.046.479361
GGCAACC17400.046.2217751
CCCAGTG8350.046.1561477
CCCCTGG21950.045.3908276
CCCTGGG13050.045.0196047
ATGGTCC5650.044.951591
GGCTCTA22700.044.5122386
GGCCACC21300.044.3829541
CGCTAGT8500.044.2321326
GGCCACT14950.044.0439831
CGCCTGG6000.043.863536
GGCAATC7300.043.8112681
CCAGTGG3550.043.6921968
GGACACC12250.043.385251
CCAGGGT5150.042.895228
GGGCACC3950.042.865231
Produced by FastQC (version 0.11.5)