##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248497_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	106210222
Sequences flagged as poor quality	0
Sequence length	100
%GC	23
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77346827313853	34.0	31.0	34.0	31.0	34.0
2	32.95948374912539	34.0	31.0	34.0	31.0	34.0
3	33.02812820596496	34.0	31.0	34.0	31.0	34.0
4	36.38848938664303	37.0	37.0	37.0	35.0	37.0
5	36.16573050755887	37.0	35.0	37.0	35.0	37.0
6	36.09825189895564	37.0	35.0	37.0	35.0	37.0
7	35.975322073990206	37.0	35.0	37.0	35.0	37.0
8	35.96252380491211	37.0	35.0	37.0	35.0	37.0
9	37.70909268036367	39.0	37.0	39.0	35.0	39.0
10-11	37.73900994199974	39.0	37.0	39.0	35.0	39.0
12-13	37.615550248073106	39.0	37.0	39.0	35.0	39.0
14-15	38.70762205920255	40.0	38.0	41.0	34.5	41.0
16-17	38.99882559797305	40.0	38.0	41.0	36.0	41.0
18-19	38.99179970643504	40.0	38.5	41.0	35.0	41.0
20-21	38.680389543861416	40.0	38.0	41.0	34.5	41.0
22-23	38.28966650215645	39.5	37.0	41.0	34.0	41.0
24-25	38.08875355707288	39.0	37.0	41.0	34.0	41.0
26-27	38.20363015058946	40.0	37.0	41.0	34.0	41.0
28-29	38.23870797012363	40.0	37.0	41.0	34.0	41.0
30-31	38.072244976571085	40.0	37.0	41.0	34.0	41.0
32-33	38.00267341499389	40.0	37.0	41.0	34.0	41.0
34-35	37.93082345219088	40.0	36.5	41.0	33.0	41.0
36-37	37.881310614339924	40.0	36.0	41.0	33.0	41.0
38-39	37.81631028885336	40.0	36.0	41.0	33.0	41.0
40-41	37.72932743705215	40.0	36.0	41.0	33.0	41.0
42-43	37.657062876678665	40.0	35.5	41.0	33.0	41.0
44-45	37.65161077433771	40.0	36.0	41.0	33.0	41.0
46-47	37.62866552524483	40.0	35.5	41.0	33.0	41.0
48-49	37.60587606153389	40.0	35.5	41.0	33.0	41.0
50-51	37.48111315029546	40.0	35.0	41.0	33.0	41.0
52-53	37.30233626665426	39.0	35.0	41.0	32.0	41.0
54-55	37.11194146171731	39.0	35.0	41.0	31.5	41.0
56-57	36.91514683021753	39.0	35.0	41.0	31.0	41.0
58-59	36.696367525717065	39.0	35.0	41.0	31.0	41.0
60-61	36.45853770082507	38.0	35.0	41.0	31.0	41.0
62-63	36.20894325500986	37.5	35.0	41.0	30.0	41.0
64-65	35.92809254743861	37.0	35.0	40.0	30.0	41.0
66-67	35.68323494795068	36.5	35.0	40.0	30.0	41.0
68-69	35.44172284565981	36.0	34.5	39.5	29.5	41.0
70-71	35.156904257294556	36.0	34.0	39.0	29.0	41.0
72-73	34.84216441521043	35.0	34.0	39.0	29.0	41.0
74-75	34.53134329669323	35.0	34.0	37.5	29.0	40.5
76-77	34.34886753649757	35.0	34.0	37.0	29.0	39.5
78-79	34.11527255352126	35.0	34.0	37.0	29.0	39.0
80-81	33.86859201744254	35.0	34.0	36.0	29.0	39.0
82-83	33.63110777604815	35.0	34.0	36.0	29.0	38.0
84-85	33.43579324219848	35.0	34.0	36.0	29.0	37.0
86-87	33.29460666224763	35.0	34.0	36.0	29.0	37.0
88-89	33.181776058240416	35.0	34.0	35.0	29.0	36.5
90-91	33.03776246696857	35.0	33.0	35.0	29.0	36.0
92-93	32.934943248682785	35.0	33.0	35.0	28.0	36.0
94-95	32.8772163144523	35.0	33.0	35.0	28.5	36.0
96-97	32.85469610448607	35.0	33.5	35.0	29.0	36.0
98-99	32.81064530210661	35.0	33.0	35.0	29.0	36.0
100	31.67369937330514	35.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0781379852004278
1101	2	0.086945422536715
1101	3	0.053576691490803796
1101	4	0.0016091379884031198
1101	5	-0.0037910508495073714
1101	6	0.0412651559404793
1101	7	0.0568283004139829
1101	8	9.364223230008406E-4
1101	9	0.1015047367879518
1101	10-11	0.11503030577176077
1101	12-13	0.2513398657499053
1101	14-15	0.2872447905330091
1101	16-17	0.228968659138566
1101	18-19	0.1081472403990773
1101	20-21	0.17079077545118793
1101	22-23	0.21804350652073623
1101	24-25	0.10076055552423213
1101	26-27	0.01060629230513399
1101	28-29	-0.033397819104166615
1101	30-31	0.06069324408193211
1101	32-33	0.1266261176421537
1101	34-35	0.08731204005061954
1101	36-37	0.07645933227510682
1101	38-39	0.018627867214306093
1101	40-41	0.01108201918684415
1101	42-43	-4.2497903599780784E-4
1101	44-45	0.04438674623798278
1101	46-47	-0.0026935923056683464
1101	48-49	0.01999081086479748
1101	50-51	-0.08664695724105798
1101	52-53	0.042477238616740465
1101	54-55	0.0809481388685711
1101	56-57	0.13711857886811885
1101	58-59	0.07008872787879028
1101	60-61	0.007320538888301087
1101	62-63	-0.011643423074211512
1101	64-65	0.09674814733091353
1101	66-67	0.02743781736600681
1101	68-69	0.05665968801639565
1101	70-71	0.07525261443384323
1101	72-73	0.07333337029068332
1101	74-75	0.11759378906369733
1101	76-77	0.14693493377121314
1101	78-79	0.11024931635085977
1101	80-81	0.09170877063059635
1101	82-83	0.08585857580989398
1101	84-85	0.05148623225039728
1101	86-87	0.025217623594294025
1101	88-89	-0.0341671049181258
1101	90-91	-0.08681651439692217
1101	92-93	0.04258274905166104
1101	94-95	0.0446216837825375
1101	96-97	0.03442987392972441
1101	98-99	-0.05995958450529315
1101	100	-0.10926643327751506
1102	1	0.13047908643339667
1102	2	0.11711450581066174
1102	3	0.08557173109076643
1102	4	0.035327156811540306
1102	5	0.04945500814427817
1102	6	0.09050531812293627
1102	7	0.08548710658722314
1102	8	0.12429394278704109
1102	9	0.16552321999219544
1102	10-11	0.12038176837130976
1102	12-13	0.23889376042734511
1102	14-15	0.4735627441299002
1102	16-17	0.3018811017378056
1102	18-19	0.30804827964094983
1102	20-21	0.4396214232817002
1102	22-23	0.46781320283483296
1102	24-25	0.38536262905648044
1102	26-27	0.3554227292000931
1102	28-29	0.38994861068821507
1102	30-31	0.42334744378744915
1102	32-33	0.38150810323351436
1102	34-35	0.39811525398408065
1102	36-37	0.36526260462645865
1102	38-39	0.2963851179197121
1102	40-41	0.307771340011989
1102	42-43	0.3294163374348571
1102	44-45	0.24038466817913928
1102	46-47	0.25756067623638046
1102	48-49	0.2837771322019691
1102	50-51	0.28771715704115763
1102	52-53	0.3173664859675256
1102	54-55	0.30414334310155056
1102	56-57	0.2806995927716329
1102	58-59	0.2838855819801367
1102	60-61	0.29392731183943255
1102	62-63	0.2900274354416439
1102	64-65	0.27550824062294055
1102	66-67	0.2565147128649983
1102	68-69	0.2807298534306639
1102	70-71	0.27431839198285957
1102	72-73	0.2924442333271742
1102	74-75	0.2776840409137904
1102	76-77	0.23474789048727018
1102	78-79	0.24171912363085823
1102	80-81	0.23681469090541185
1102	82-83	0.24295367646053734
1102	84-85	0.29349974612766516
1102	86-87	0.4339971710540098
1102	88-89	0.38332146685262103
1102	90-91	0.34289277840902344
1102	92-93	0.33977301311367825
1102	94-95	0.33911641503363654
1102	96-97	0.2348130433673461
1102	98-99	0.1875890663538229
1102	100	0.22181525460352702
1103	1	0.08378299069746475
1103	2	0.07715609658642109
1103	3	0.0513991616722862
1103	4	0.0071160049154315175
1103	5	0.027488479484595985
1103	6	0.05755261079823981
1103	7	0.08393048467914355
1103	8	-0.025998758443357417
1103	9	0.04185583748741806
1103	10-11	0.06434559034949672
1103	12-13	0.16923979016813462
1103	14-15	0.22340051585145204
1103	16-17	0.24622561239981877
1103	18-19	0.2188523995993208
1103	20-21	0.22730799615444397
1103	22-23	0.15426219066107905
1103	24-25	0.18127324319314653
1103	26-27	0.11953895710552587
1103	28-29	0.1093132158230361
1103	30-31	0.1335898106381137
1103	32-33	0.21278634037188482
1103	34-35	0.15414839164292715
1103	36-37	0.12915998646575133
1103	38-39	0.1111962469908292
1103	40-41	0.06911983886929107
1103	42-43	0.12099900707193711
1103	44-45	0.1664618698588285
1103	46-47	0.2394344400509283
1103	48-49	0.23139701079494301
1103	50-51	0.23016158488891847
1103	52-53	0.24364511173226333
1103	54-55	0.2516491054768579
1103	56-57	0.24952144520614894
1103	58-59	0.2517875543035757
1103	60-61	0.2488588013277493
1103	62-63	0.2402946196036666
1103	64-65	0.25682019739956985
1103	66-67	0.23976605824703512
1103	68-69	0.2401159128210466
1103	70-71	0.2185329241434104
1103	72-73	0.2453048517184513
1103	74-75	0.21142726138055679
1103	76-77	0.1659667732651755
1103	78-79	0.1413707289220838
1103	80-81	0.17151983764609469
1103	82-83	0.13636500326234113
1103	84-85	0.17870907049562845
1103	86-87	0.28248925347912035
1103	88-89	0.16507375575828576
1103	90-91	0.19926501136367847
1103	92-93	0.1413140020613568
1103	94-95	0.20690416593414085
1103	96-97	0.323054503554836
1103	98-99	0.2551086279438408
1103	100	0.3598766767363841
1104	1	0.09931182646171521
1104	2	0.08294852765856575
1104	3	0.05179267355703843
1104	4	0.029964559510077038
1104	5	0.03882805711823778
1104	6	0.03218337958531947
1104	7	0.07704980437409858
1104	8	0.08659641874699275
1104	9	0.1338604555018108
1104	10-11	0.1125440789695844
1104	12-13	0.19112634070787493
1104	14-15	0.2902750353449477
1104	16-17	0.25273697806368034
1104	18-19	0.2550372362920328
1104	20-21	0.37699594959840255
1104	22-23	0.31780397837604113
1104	24-25	0.4266989937631678
1104	26-27	0.2678659040549931
1104	28-29	0.2009350372702059
1104	30-31	0.22175746870928492
1104	32-33	0.3196978225676972
1104	34-35	0.29336605507911173
1104	36-37	0.3024092488359855
1104	38-39	0.33167480467174926
1104	40-41	0.12865604390076868
1104	42-43	0.21224272648462517
1104	44-45	0.23975634181516625
1104	46-47	0.23664801665071167
1104	48-49	0.22517771312369206
1104	50-51	0.22728157217791534
1104	52-53	0.23262902718842327
1104	54-55	0.23249001649594447
1104	56-57	0.20650118939543205
1104	58-59	0.20406962060452116
1104	60-61	0.20972078865555943
1104	62-63	0.2076933653957056
1104	64-65	0.18595259165227418
1104	66-67	0.19898689279690984
1104	68-69	0.21026604722168685
1104	70-71	0.20526459037021283
1104	72-73	0.21106523199497929
1104	74-75	0.17778734866082146
1104	76-77	0.166088926259782
1104	78-79	0.17998238374424602
1104	80-81	0.21066411454837208
1104	82-83	0.1450508968055999
1104	84-85	0.15628375519902704
1104	86-87	0.16780667304553987
1104	88-89	0.15297616734621755
1104	90-91	0.22239363944518686
1104	92-93	0.21214464475420414
1104	94-95	0.1725310007210723
1104	96-97	0.27024992969945316
1104	98-99	0.3108888793699265
1104	100	0.30114559168765354
1105	1	0.0913738335236971
1105	2	0.0802799191791479
1105	3	0.057810695835271986
1105	4	0.036336585968584245
1105	5	0.035158742836550516
1105	6	0.05398956652684461
1105	7	0.06478106103766379
1105	8	0.09331454876777201
1105	9	0.11057643573601439
1105	10-11	0.12251950757613628
1105	12-13	0.24332893078286588
1105	14-15	0.21726311683798372
1105	16-17	0.15684693762226232
1105	18-19	0.22050036486349
1105	20-21	0.2516710212921609
1105	22-23	0.18861822154349994
1105	24-25	0.18998834782110663
1105	26-27	0.20371689858006192
1105	28-29	0.21075655098210433
1105	30-31	0.2023133079046886
1105	32-33	0.24327005088441211
1105	34-35	0.10104914236971041
1105	36-37	0.01906020412746301
1105	38-39	0.12914451776030234
1105	40-41	0.21889162175063603
1105	42-43	0.12809835759529875
1105	44-45	0.13944933971376372
1105	46-47	0.12597262302119105
1105	48-49	0.12423216725154163
1105	50-51	0.10708289438714758
1105	52-53	0.1443076443446074
1105	54-55	0.13990135146661942
1105	56-57	0.08470312912921685
1105	58-59	0.043835528303695526
1105	60-61	0.12801162964733948
1105	62-63	0.13755842483544
1105	64-65	0.122652976247565
1105	66-67	0.11438482027256924
1105	68-69	0.12002348218917547
1105	70-71	0.11735023689700341
1105	72-73	0.12796149654436562
1105	74-75	0.13110345498269993
1105	76-77	0.11274921208541144
1105	78-79	0.21332527280264912
1105	80-81	0.17529002086044443
1105	82-83	0.198145174941196
1105	84-85	0.23975499624954466
1105	86-87	0.29719086486745994
1105	88-89	0.12175135673557236
1105	90-91	0.10692662280724363
1105	92-93	0.1766498989299734
1105	94-95	0.2722896884267243
1105	96-97	0.3086932163764118
1105	98-99	0.23799920513953055
1105	100	0.23828933204745795
1106	1	0.08369872761512909
1106	2	0.056073922326426384
1106	3	0.03276202973079023
1106	4	0.021568480470108398
1106	5	0.05990592111903226
1106	6	0.027233856943240653
1106	7	0.04910241478537358
1106	8	4.839507412910393E-4
1106	9	0.053579137943351896
1106	10-11	0.10405373217106018
1106	12-13	0.12322178712476983
1106	14-15	0.011752205007212524
1106	16-17	0.10713038846438394
1106	18-19	0.15398739589151944
1106	20-21	0.08203817734066376
1106	22-23	0.03760551514513111
1106	24-25	0.10710640313399011
1106	26-27	0.20941819276003315
1106	28-29	0.28714738243355953
1106	30-31	0.026179803663460177
1106	32-33	-0.019280376779050812
1106	34-35	0.021287956497438643
1106	36-37	0.10641899724529935
1106	38-39	0.1868102904469069
1106	40-41	0.1523375589550895
1106	42-43	0.06503405071990898
1106	44-45	0.1586724763320646
1106	46-47	0.1792843405727993
1106	48-49	0.15935405056723795
1106	50-51	0.1498510334718901
1106	52-53	0.17088185406315404
1106	54-55	0.17542611473054137
1106	56-57	0.1746344145429788
1106	58-59	0.18036075404665297
1106	60-61	0.16909690857750803
1106	62-63	0.16465816828544177
1106	64-65	0.1740864498792689
1106	66-67	0.17896227957853483
1106	68-69	0.16693002814582059
1106	70-71	0.1628360209388191
1106	72-73	0.15618850744308332
1106	74-75	0.17638595412012847
1106	76-77	0.16290105247224318
1106	78-79	0.17694095113682096
1106	80-81	0.1778580780707273
1106	82-83	0.13477235415012245
1106	84-85	0.16937062206822162
1106	86-87	0.06510848948875747
1106	88-89	0.10704193408303553
1106	90-91	0.007759250034546028
1106	92-93	0.1667059745310624
1106	94-95	0.16182361799566536
1106	96-97	0.10641274610801332
1106	98-99	0.19470324063144062
1106	100	0.21662451022708495
1107	1	0.03226646592334248
1107	2	0.01777624665316324
1107	3	0.019240690059262988
1107	4	0.02763022664843362
1107	5	0.004568923320491081
1107	6	-0.016520121471039317
1107	7	0.003962295198263632
1107	8	-0.0016212044082450916
1107	9	-0.016189372389582957
1107	10-11	0.05835848694513146
1107	12-13	0.02568621648092062
1107	14-15	-0.1291009817097546
1107	16-17	0.05714072395515757
1107	18-19	0.1367926614719508
1107	20-21	0.12187451091798351
1107	22-23	0.17881420856226526
1107	24-25	0.15059488368144258
1107	26-27	0.14101589409833792
1107	28-29	0.1634018059853588
1107	30-31	0.10184477082307808
1107	32-33	0.07827724179513496
1107	34-35	0.1254294604096131
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2216	18-19	-0.578093095696083
2216	20-21	-0.7714825852309488
2216	22-23	-0.8791868836718066
2216	24-25	-0.9686297071246202
2216	26-27	-0.6148196796159127
2216	28-29	-0.5399988354490333
2216	30-31	-0.6663846627223649
2216	32-33	-0.8514783173156104
2216	34-35	-0.6994992453927367
2216	36-37	-0.7928383352357429
2216	38-39	-0.6096668602312718
2216	40-41	-0.3974863261769528
2216	42-43	-0.4542205808236943
2216	44-45	-0.3535811981559007
2216	46-47	-0.24798370445694928
2216	48-49	-0.22345973747065528
2216	50-51	-0.2807733363603546
2216	52-53	-0.2794458798112629
2216	54-55	-0.2802096953656701
2216	56-57	-0.30419275302906357
2216	58-59	-0.27409182947081234
2216	60-61	-0.274940584787565
2216	62-63	-0.3004197539639719
2216	64-65	-0.2932585387370352
2216	66-67	-0.2695427163194495
2216	68-69	-0.26110682649895267
2216	70-71	-0.2474566532571103
2216	72-73	-0.2586456025960189
2216	74-75	-0.24259975230118158
2216	76-77	-0.26652263235856566
2216	78-79	-0.28642821857294365
2216	80-81	-0.28284017437845677
2216	82-83	-0.27737418843575057
2216	84-85	-0.24608268326591087
2216	86-87	-0.26188267791837205
2216	88-89	-0.2932042761564446
2216	90-91	-0.30643168847029756
2216	92-93	-0.33432252632247383
2216	94-95	-0.3026221909059785
2216	96-97	-0.18589738029908176
2216	98-99	-0.2566663304474517
2216	100	-0.3094312037129612
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	89.0
8	539.0
9	1454.0
10	3280.0
11	6457.0
12	10684.0
13	15297.0
14	21596.0
15	30923.0
16	43004.0
17	61359.0
18	85959.0
19	118520.0
20	161433.0
21	218446.0
22	287652.0
23	374175.0
24	474396.0
25	592272.0
26	731292.0
27	901237.0
28	1120610.0
29	1403781.0
30	1786298.0
31	2333410.0
32	3177101.0
33	4585620.0
34	6995608.0
35	1.3653386E7
36	1.1403079E7
37	1.8953673E7
38	2.4174124E7
39	1.2148423E7
40	335040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.92831370922842	29.517674405860365	30.69488624534938	27.85912563956183
2	9.023796511222029	32.249639971104656	33.01611814209478	25.710445375578534
3	7.640318745518973	28.70856778295041	33.41758617318114	30.23352729834948
4	6.9722075685482245	28.05544960498385	35.945797315150074	29.02654551131785
5	5.590708771963126	28.139914032016172	37.47400064586809	28.795376550152614
6	5.355055201240883	23.204868556743083	34.48333772603749	36.95673851597854
7	6.581256369090351	26.86157176095536	32.174614040445185	34.3825578295091
8	5.385976287290633	26.72658711160737	31.639159978536117	36.248276622565875
9	6.061375147111547	27.692979494949178	30.8866881005107	35.35895725742858
10-11	4.491063958043511	46.45441942490244	34.35782292216657	14.696693694887482
12-13	4.03101448449514	45.73824362924177	34.622913625371375	15.607828260891713
14-15	4.070309641815011	43.75798620550124	34.892808023671556	17.27889612901219
16-17	4.337998415413638	43.051601326402114	35.109301179600635	17.50109907858362
18-19	4.394781904256871	42.37328785891076	35.49271749239688	17.739212744435502
20-21	4.098919597246306	42.058157239665306	36.13405362286333	17.708869540225063
22-23	4.125060109562712	41.64102773459978	36.399892375707495	17.834019780130014
24-25	4.145025726166223	41.33119204436948	36.3723609859403	18.15142124352401
26-27	4.4903933032085765	41.17934230737043	36.147818336084306	18.18244605333669
28-29	4.37683766445757	41.35055381015963	36.327315086489506	17.94529343889329
30-31	4.313592193524859	41.56963237196874	36.44041237081565	17.676363063690754
32-33	4.319171491398581	41.41971687300635	36.472796464151905	17.788315171443156
34-35	4.364074789734654	41.22850378312478	36.537891260871234	17.869530166269335
36-37	4.462385219761225	41.32612510116501	36.31931398111921	17.89217569795456
38-39	4.51763406326078	41.66618083674375	36.32393945399194	17.49224564600353
40-41	4.5291734671472	41.88046280266648	36.301811131396185	17.288552598790137
42-43	4.565806315461305	41.682263614604146	36.44559458224424	17.30633548769031
44-45	4.694075581219824	41.60085257263342	36.31971272265352	17.385359123493238
46-47	4.781392522317847	41.741089932384476	36.21888447134651	17.25863307395116
48-49	4.779692792661874	41.78685282026963	36.331875185890574	17.101579201177916
50-51	4.79790079956108	41.721656741716764	36.474408870776976	17.006033587945186
52-53	4.82648991223781	41.63282778662868	36.500863251282986	17.039819049850525
54-55	4.875254125322708	41.46296080537805	36.44986627007304	17.2119187992262
56-57	4.973781210282585	41.44767020732229	36.3757369524074	17.20281162998772
58-59	4.994125797119683	41.545335119982454	36.53378281364679	16.92675626925107
60-61	5.049223650105942	41.63542137758767	36.427187423634564	16.888167548671834
62-63	5.113352634278585	41.426166822942506	36.32701527700697	17.13346526577194
64-65	5.252572581949786	41.30018295225859	36.19413816873483	17.253106297056796
66-67	5.303051212828473	41.32767109494137	36.185863848798554	17.1834138434316
68-69	5.378996585808721	41.62387181994109	36.11726321849749	16.8798683757527
70-71	5.390214700803469	41.900451916953905	36.02557670955626	16.683756672686364
72-73	5.454530544150449	41.67872325885921	36.057575512835285	16.809170684155053
74-75	5.526626738086597	41.38243841248368	36.01347117047487	17.077463678954853
76-77	5.649094179244336	41.45311905836234	35.91434385452033	16.983442907872988
78-79	5.673839472814585	41.68832214661975	35.87633071701893	16.761507663546734
80-81	5.639987628295237	41.60311919487883	36.026665543107484	16.73022763371845
82-83	5.697514287801546	41.31635103054444	36.098783080978755	16.88735160067526
84-85	5.855923709337756	41.23034696042504	35.89324492781472	17.02048440242248
86-87	5.955879955091789	41.390680349962935	35.66678114561114	16.986658549334134
88-89	5.989912114385267	41.67340760199742	35.59476592112951	16.741914362487808
90-91	5.985912986605997	41.588089856165524	35.69144235083292	16.734554806395554
92-93	6.050260774245463	41.26725176902907	35.75153178011814	16.930955676607322
94-95	6.172249597595229	41.13406428997013	35.6995920788114	16.99409403362324
96-97	6.291485996778046	41.28005284328682	35.52103729102394	16.907423868911195
98-99	6.362445278099325	41.3282270073111	35.52693724816571	16.78239046642386
100	6.320352868104371	41.06798133351	35.65165814278251	16.960007655603132
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1366101.0
1	2536775.5
2	2917558.5
3	1300283.0
4	310622.5
5	131717.0
6	117191.0
7	131047.0
8	166329.0
9	228927.0
10	332885.0
11	497386.0
12	736991.0
13	1060527.0
14	1472227.0
15	1964446.0
16	2506552.5
17	3065675.5
18	3627106.5
19	4161061.5
20	4619282.0
21	4974019.5
22	5218298.5
23	5347432.5
24	5376562.0
25	5321816.5
26	5182366.5
27	4956948.0
28	4658626.5
29	4305260.0
30	3910437.5
31	3488474.5
32	3060299.0
33	2653827.5
34	2283334.5
35	1955260.0
36	1669048.5
37	1420261.5
38	1205981.0
39	1021664.0
40	862554.5
41	727879.0
42	614101.5
43	517793.0
44	440188.0
45	378876.0
46	326880.0
47	278904.0
48	235005.0
49	203157.5
50	181953.5
51	163385.5
52	144648.0
53	125129.5
54	106837.0
55	88283.5
56	67969.5
57	48344.0
58	32460.0
59	21168.0
60	14060.5
61	9733.0
62	7114.0
63	5497.0
64	4385.0
65	3581.0
66	3053.5
67	2675.0
68	2304.0
69	1982.5
70	1714.5
71	1456.5
72	1271.0
73	1118.5
74	981.0
75	848.5
76	736.5
77	653.5
78	565.0
79	488.0
80	440.0
81	377.0
82	315.0
83	261.0
84	231.5
85	226.5
86	188.0
87	163.5
88	146.5
89	112.5
90	95.0
91	76.5
92	64.0
93	53.5
94	43.0
95	37.0
96	30.5
97	25.0
98	14.0
99	7.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006263050650623817
2	0.008496357346847463
3	0.0064758361958795274
4	0.05411249399328061
5	0.00261368439659226
6	1.7889050264860573E-5
7	0.0
8	1.638260392676705E-4
9	0.0
10-11	0.0
12-13	0.0038640348572098835
14-15	0.0019640294132894287
16-17	7.541646980080693E-4
18-19	4.524046659087107E-4
20-21	2.320868889625332E-4
22-23	0.0
24-25	3.413042484743135E-4
26-27	1.4593698900280993E-5
28-29	0.0
30-31	1.2757717425729512E-4
32-33	2.8245868839253534E-6
34-35	1.2993099666056625E-4
36-37	7.90884327499099E-5
38-39	2.014871977200085E-4
40-41	1.911303791456156E-4
42-43	3.2011984684487336E-4
44-45	3.629594145844079E-4
46-47	1.991333753167374E-4
48-49	2.1325630973636418E-4
50-51	4.23688032588803E-4
52-53	3.930883413462783E-4
54-55	7.202696554009652E-5
56-57	4.8394588611254386E-4
58-59	0.0012649441595179041
60-61	0.003965249220550542
62-63	4.943027046869368E-5
64-65	0.0
66-67	4.378109670084298E-5
68-69	3.766115845233805E-6
70-71	0.0
72-73	0.0
74-75	4.707644806542256E-7
76-77	2.4479752994019724E-5
78-79	0.0
80-81	1.1486653327963105E-4
82-83	0.0011910341360551907
84-85	0.005925983282475391
86-87	0.0027478522735787147
88-89	0.0014532499517795941
90-91	0.0011241855798022906
92-93	0.0010488632628976146
94-95	0.0
96-97	0.007433841914010876
98-99	3.766115845233805E-6
100	0.11601331555450471
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.06210222E8
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.62449757460865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.90556564015256	83.07771438186867
2	3.5313121312465263	6.117962782967017
3	0.2665616957291597	0.692723188955225
4	0.09960865660604723	0.3451419933032226
5	0.051575661261241405	0.2233857871916622
6	0.029742726026437117	0.1545869219123611
7	0.022587260253006045	0.13696270497025037
8	0.016949040212957574	0.11745616742554306
9	0.009929340144961203	0.07741116911741905
>10	0.05816714088640229	0.9432720937071777
>50	0.004562180601744284	0.2704250460519007
>100	0.002873486149922045	0.47752767087628323
>500	2.939519139303228E-4	0.17265126172215825
>1k	1.3930135189984999E-4	0.252118030966048
>5k	3.3806304190744663E-5	0.20630544605402296
>10k+	9.798115907980488E-5	6.734355352911059
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	345392	0.32519657100424854	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	259414	0.24424579396887053	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	246584	0.23216597739528308	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	242411	0.22823697703974294	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	236273	0.22245787227523167	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	219370	0.20654320824223493	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	206757	0.1946677034532514	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	206640	0.19455754456477833	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	197776	0.18621183185174023	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	191153	0.17997608554099434	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	187418	0.17645947487050728	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	185759	0.17489747832369656	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	176428	0.1661120715857274	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	175771	0.16549348705814776	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	172022	0.16196369498220237	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	164277	0.15467155317686843	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163466	0.15390797318924726	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163220	0.1536763570647654	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	151393	0.14254089403937034	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	149475	0.14073504149158073	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	143786	0.13537868323069696	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	143269	0.1348919127577005	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	143135	0.13476574787688517	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	140287	0.13208427339507867	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	140171	0.1319750560355669	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	139644	0.13147887027295735	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	131059	0.12339584414012429	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	129234	0.12167755378573637	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127553	0.12009484360177686	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121772	0.11465186467645272	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117267	0.11041027670575813	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6288451030636205E-4	0.0	0.0	1.1298347535701414E-5	0.0
2	3.7378699763945507E-4	0.0	0.0	5.0842563910656355E-5	0.0
3	5.686834926303044E-4	0.0	0.0	9.132830924691976E-5	0.0
4	8.803295788234018E-4	0.0	0.0	1.0450971470523806E-4	0.0
5	0.0015478736123910935	0.0	0.0	1.0921735951178033E-4	0.0
6	0.0028942600270621785	0.0	0.0	1.1486653327963103E-4	0.0
7	0.005641641536160239	0.0	0.0	1.2145723600879019E-4	0.0
8	0.014900637341667546	0.0	0.0	1.233402939314071E-4	0.0
9	0.04490622380960657	0.0	0.0	1.2616488081533244E-4	0.0
10-11	0.09732820255285786	0.0	0.0	1.2710640977664088E-4	0.0
12-13	0.1436212985224718	0.0	0.0	1.3981705075430498E-4	0.0
14-15	0.18913339621868036	0.0	0.0	1.8124432505187686E-4	0.0
16-17	0.22240891276924363	0.0	0.0	2.264377151946825E-4	0.0
18-19	0.25087368709200136	0.0	0.0	2.69277282934217E-4	0.0
20-21	0.2840771766770246	9.415289613084511E-7	0.0	2.8057563046991844E-4	0.0
22-23	0.3272622855453593	9.415289613084511E-7	0.0	2.885786266410402E-4	0.0
24-25	0.37353749246470835	9.415289613084511E-7	0.0	3.003477386573959E-4	0.0
26-27	0.4191894072116712	9.415289613084511E-7	0.0	3.1305837963506E-4	0.0
28-29	0.4662484369913096	9.415289613084511E-7	0.0	3.262397850933783E-4	0.0
30-31	0.5198906372684167	9.415289613084511E-7	0.0	3.337720167838459E-4	0.0
32-33	0.5873271783576537	9.415289613084511E-7	0.0	3.4601189328085577E-4	4.7076448065422555E-7
34-35	0.6621344789204942	9.415289613084511E-7	0.0	3.573102408165572E-4	9.415289613084511E-7
36-37	0.7283573891786046	9.415289613084511E-7	0.0	3.667255304296417E-4	9.415289613084511E-7
38-39	0.7916554397184106	9.415289613084511E-7	0.0	4.0815280472721353E-4	9.415289613084511E-7
40-41	0.8601912158699753	9.415289613084511E-7	0.0	4.4863855006347693E-4	9.415289613084511E-7
42-43	0.9303638401207748	9.415289613084511E-7	0.0	5.05130287741984E-4	9.415289613084511E-7
44-45	1.0045200734068704	9.415289613084511E-7	0.0	5.404376237910509E-4	9.415289613084511E-7
46-47	1.0812518591666254	9.415289613084511E-7	0.0	5.644466123044164E-4	9.415289613084511E-7
48-49	1.16154544898701	9.415289613084511E-7	0.0	6.430642805736721E-4	9.415289613084511E-7
50-51	1.2468154901323905	9.415289613084511E-7	0.0	7.53693933527415E-4	9.415289613084511E-7
52-53	1.3387035383468082	9.415289613084511E-7	0.0	8.676189378457376E-4	9.415289613084511E-7
54-55	1.4372486670821571	9.415289613084511E-7	0.0	9.528273088441526E-4	9.415289613084511E-7
56-57	1.5411002530434406	9.415289613084511E-7	0.0	9.942545831417243E-4	9.415289613084511E-7
58-59	1.651563255371032	9.415289613084511E-7	0.0	0.0010253250388649032	9.415289613084511E-7
60-61	1.7681334853061506	9.415289613084511E-7	0.0	0.001077109131736868	9.415289613084511E-7
62-63	1.8918226157177225	9.415289613084511E-7	0.0	0.001133600869415375	1.4122934419626767E-6
64-65	2.0226523017718576	9.415289613084511E-7	0.0	0.0012079816573587429	1.8830579226169022E-6
66-67	2.1606823305575995	9.415289613084511E-7	0.0	0.0013181405458318313	1.8830579226169022E-6
68-69	2.305772414259712	9.415289613084511E-7	0.0	0.0013802814572781894	1.8830579226169022E-6
70-71	2.458959647029078	9.415289613084511E-7	0.0	0.0014320655501501541	1.8830579226169022E-6
72-73	2.621236871155396	9.415289613084511E-7	0.0	0.0014852619364640815	1.8830579226169022E-6
74-75	2.7924303745453054	9.415289613084511E-7	0.0	0.001548344376871748	2.8245868839253534E-6
76-77	2.9712775668616906	9.415289613084511E-7	0.0	0.0017140534740620352	2.8245868839253534E-6
78-79	3.1575270598718834	9.415289613084511E-7	0.0	0.002041234788116722	2.8245868839253534E-6
80-81	3.352566196500371	9.415289613084511E-7	0.0	0.0023622961639229037	2.8245868839253534E-6
82-83	3.557626496628545	9.415289613084511E-7	0.0	0.0024272616622531868	2.8245868839253534E-6
84-85	3.7717113518508603	9.415289613084511E-7	0.0	0.002464922820705525	2.8245868839253534E-6
86-87	3.9933171404161083	9.415289613084511E-7	0.0	0.002485636457854311	2.8245868839253534E-6
88	4.164480514879255	9.415289613084511E-7	0.0	0.002500700921235246	2.8245868839253534E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCATCC	1065	0.0	47.695114	1
CCCAGGT	970	0.0	47.485012	7
GGCCAGC	200	0.0	47.032684	1
CCCTGTG	1920	0.0	46.51088	7
GAGTACC	425	0.0	46.47936	1
GGCAACC	1740	0.0	46.221775	1
CCCAGTG	835	0.0	46.156147	7
CCCCTGG	2195	0.0	45.390827	6
CCCTGGG	1305	0.0	45.019604	7
ATGGTCC	565	0.0	44.95159	1
GGCTCTA	2270	0.0	44.512238	6
GGCCACC	2130	0.0	44.382954	1
CGCTAGT	850	0.0	44.232132	6
GGCCACT	1495	0.0	44.043983	1
CGCCTGG	600	0.0	43.86353	6
GGCAATC	730	0.0	43.811268	1
CCAGTGG	355	0.0	43.692196	8
GGACACC	1225	0.0	43.38525	1
CCAGGGT	515	0.0	42.89522	8
GGGCACC	395	0.0	42.86523	1
>>END_MODULE