##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248496_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23375132
Sequences flagged as poor quality	0
Sequence length	100
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.800380293039627	33.0	31.0	34.0	26.0	34.0
2	31.033650547941292	34.0	31.0	34.0	27.0	34.0
3	31.16004247590987	34.0	31.0	34.0	27.0	34.0
4	34.505630342536676	37.0	35.0	37.0	30.0	37.0
5	34.441714382618244	37.0	35.0	37.0	30.0	37.0
6	34.396867833730305	37.0	35.0	37.0	30.0	37.0
7	34.40255601551255	37.0	35.0	37.0	30.0	37.0
8	34.3678874198443	37.0	35.0	37.0	30.0	37.0
9	35.860054608461674	39.0	37.0	39.0	30.0	39.0
10-11	35.99628010656795	39.0	37.0	39.0	30.0	39.0
12-13	35.99578776710223	39.0	37.0	39.0	30.0	39.0
14-15	37.09272371167786	40.0	37.0	41.0	30.0	41.0
16-17	36.926465335040675	40.0	37.0	41.0	30.0	41.0
18-19	36.658463468783836	39.0	36.5	41.0	30.0	41.0
20-21	36.49639184925245	39.0	35.0	41.0	29.5	41.0
22-23	36.378654546207486	39.0	35.0	41.0	28.5	41.0
24-25	36.24777887885296	39.0	35.0	41.0	27.5	41.0
26-27	36.0921425812697	39.0	35.0	41.0	27.0	41.0
28-29	35.96089498018664	39.0	35.0	41.0	25.5	41.0
30-31	35.88845585984285	39.0	35.0	41.0	25.0	41.0
32-33	35.790460605741174	39.0	35.0	41.0	25.0	41.0
34-35	35.66531423223621	39.0	35.0	40.5	25.0	41.0
36-37	35.55066891173064	39.0	35.0	40.0	25.0	41.0
38-39	35.37374257394568	38.5	34.5	40.0	25.0	41.0
40-41	35.153750554221475	38.0	34.0	40.0	25.0	41.0
42-43	34.8751459029194	38.0	33.5	40.0	25.0	41.0
44-45	34.65204878843037	38.0	33.0	40.0	25.0	41.0
46-47	34.47797062707496	37.0	33.0	40.0	25.0	41.0
48-49	34.36848609026037	37.0	33.0	39.5	25.0	41.0
50-51	34.086940771072435	36.0	33.0	39.0	25.0	41.0
52-53	33.72913218201292	35.5	32.5	39.0	25.0	41.0
54-55	33.546695714916176	35.0	32.0	39.0	25.0	40.5
56-57	33.409398351205034	35.0	32.0	37.5	25.0	40.0
58-59	33.12613631443878	35.0	32.0	37.0	25.0	40.0
60-61	32.84605481586158	35.0	32.0	36.5	25.0	39.5
62-63	32.58617557753257	35.0	32.0	36.0	25.0	39.0
64-65	32.42438162488237	35.0	32.0	35.5	25.0	39.0
66-67	32.284141218967235	35.0	32.0	35.0	24.0	38.0
68-69	32.12640827012228	35.0	31.0	35.0	24.0	37.0
70-71	31.958215701199038	35.0	31.0	35.0	24.0	37.0
72-73	31.785427350741806	35.0	31.0	35.0	23.0	36.0
74-75	31.60384041895464	35.0	31.0	35.0	23.0	36.0
76-77	31.392760177782097	35.0	31.0	35.0	22.5	36.0
78-79	31.20526215638055	35.0	31.0	35.0	20.5	36.0
80-81	30.980651595892592	35.0	30.0	35.0	20.0	36.0
82-83	30.775040735598843	35.0	30.0	35.0	19.5	36.0
84-85	30.565274219627938	35.0	30.0	35.0	17.5	36.0
86-87	30.284497302517906	35.0	29.0	35.0	16.0	36.0
88-89	29.97386985878839	34.5	29.0	35.0	12.5	36.0
90-91	29.521932068661688	34.0	28.0	35.0	10.0	35.0
92-93	28.96004138072889	34.0	26.5	35.0	8.0	35.0
94-95	28.231254159334803	34.0	25.0	35.0	7.5	35.0
96-97	27.312573978191867	33.0	22.0	35.0	7.0	35.0
98-99	26.127417376723262	33.0	17.0	35.0	7.0	35.0
100	24.404365973206055	30.0	10.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5183183729610015
1101	2	0.5105013463483346
1101	3	0.47846918010314
1101	4	0.4192695134719102
1101	5	0.3987841922521582
1101	6	0.3490549029479766
1101	7	0.3777120828421374
1101	8	0.393183013402826
1101	9	0.44609640164842546
1101	10-11	0.4953927209937419
1101	12-13	0.48484312097338744
1101	14-15	0.6268374348461236
1101	16-17	0.6391746807618546
1101	18-19	0.6346361630819715
1101	20-21	0.665959441308118
1101	22-23	0.6497233269023255
1101	24-25	0.6501392300306605
1101	26-27	0.6931035627517872
1101	28-29	0.6668223125633972
1101	30-31	0.6600912724956771
1101	32-33	0.6715170817597311
1101	34-35	0.6520475865590214
1101	36-37	0.6350026325772831
1101	38-39	0.6401593548953457
1101	40-41	0.642542298499805
1101	42-43	0.6425474620268403
1101	44-45	0.6705299651471748
1101	46-47	0.7120161773574267
1101	48-49	0.6612528359399263
1101	50-51	0.644695557153689
1101	52-53	0.6310801103979955
1101	54-55	0.580813012474259
1101	56-57	0.5927550451894561
1101	58-59	0.5928536954580821
1101	60-61	0.5614927930682256
1101	62-63	0.5842394980805494
1101	64-65	0.5671727023024289
1101	66-67	0.5507405353096004
1101	68-69	0.5718689913496249
1101	70-71	0.5735446413591561
1101	72-73	0.5579796489677697
1101	74-75	0.5906689104467304
1101	76-77	0.5740125172949817
1101	78-79	0.6161391852886915
1101	80-81	0.5832270073528285
1101	82-83	0.5643299330076879
1101	84-85	0.4940535764439069
1101	86-87	0.5169311059940895
1101	88-89	0.46487497340186223
1101	90-91	0.4196618193499866
1101	92-93	0.37176096987890617
1101	94-95	0.39859877468684957
1101	96-97	0.3693597079005855
1101	98-99	0.3322672567074072
1101	100	0.35098529738360185
1102	1	0.3650054042271087
1102	2	0.3524644939053303
1102	3	0.3119285779333225
1102	4	0.28847985713693447
1102	5	0.2827951094591583
1102	6	0.23772044996449182
1102	7	0.26155021469703854
1102	8	0.2845424470723117
1102	9	0.2106973106212635
1102	10-11	0.3618432450949669
1102	12-13	0.41045498419961746
1102	14-15	0.5683660497529033
1102	16-17	0.602539273939648
1102	18-19	0.6225387386471368
1102	20-21	0.589215392926377
1102	22-23	0.5718106828300762
1102	24-25	0.5558345605760024
1102	26-27	0.584715362355702
1102	28-29	0.583943015806355
1102	30-31	0.5796168464421214
1102	32-33	0.5652639300351368
1102	34-35	0.6323136905040982
1102	36-37	0.5792794790996467
1102	38-39	0.6306474324809486
1102	40-41	0.635130161446547
1102	42-43	0.6536985175010557
1102	44-45	0.6643867762980378
1102	46-47	0.6477671332732271
1102	48-49	0.6163818082799608
1102	50-51	0.5913490230637564
1102	52-53	0.574056597936142
1102	54-55	0.5258944283811502
1102	56-57	0.5541532458639935
1102	58-59	0.5403687483150819
1102	60-61	0.507024823275728
1102	62-63	0.4977062636715104
1102	64-65	0.4803893461179882
1102	66-67	0.4623787769720309
1102	68-69	0.47705797249034987
1102	70-71	0.483922217242025
1102	72-73	0.4484014165230157
1102	74-75	0.4927233421445436
1102	76-77	0.498624386584666
1102	78-79	0.5093994726244659
1102	80-81	0.45557679316274147
1102	82-83	0.4577273666403592
1102	84-85	0.41300401562164524
1102	86-87	0.4291637553866039
1102	88-89	0.3921754998468572
1102	90-91	0.34097286179125774
1102	92-93	0.29986440979629947
1102	94-95	0.30895970037611775
1102	96-97	0.40868775729373397
1102	98-99	0.40161787852555264
1102	100	0.3713563135060305
1103	1	0.3708073441328743
1103	2	0.39116622800496614
1103	3	0.36959068789601446
1103	4	0.35025379828492476
1103	5	0.3491214523266635
1103	6	0.32235990219629684
1103	7	0.33329986188883254
1103	8	0.3167396742697477
1103	9	0.3571750123517461
1103	10-11	0.4726791878730978
1103	12-13	0.5084036455027103
1103	14-15	0.6237771240301626
1103	16-17	0.6719250373664707
1103	18-19	0.6558637478482297
1103	20-21	0.6436639377763029
1103	22-23	0.6242892797273498
1103	24-25	0.5973926845384696
1103	26-27	0.6212573742475485
1103	28-29	0.6248698153700616
1103	30-31	0.6195422081397552
1103	32-33	0.6396272370026068
1103	34-35	0.6358033238677478
1103	36-37	0.617352193891449
1103	38-39	0.618401397270695
1103	40-41	0.6255933404882938
1103	42-43	0.6441446214136448
1103	44-45	0.6582437555778355
1103	46-47	0.654411759562457
1103	48-49	0.601359272606139
1103	50-51	0.5523148679621457
1103	52-53	0.5620893448256084
1103	54-55	0.5505083926825378
1103	56-57	0.5508320608694817
1103	58-59	0.5435484607929766
1103	60-61	0.495178908916003
1103	62-63	0.504613233379267
1103	64-65	0.482244100241239
1103	66-67	0.45689194549086665
1103	68-69	0.47251649718377564
1103	70-71	0.4779060128452457
1103	72-73	0.4546638688186029
1103	74-75	0.4993862123719808
1103	76-77	0.5113682059655389
1103	78-79	0.5441001711549518
1103	80-81	0.4909588119530781
1103	82-83	0.45989914774813556
1103	84-85	0.4199984248230848
1103	86-87	0.4032453844703099
1103	88-89	0.35979395807698467
1103	90-91	0.36541310507194424
1103	92-93	0.34639769305906754
1103	94-95	0.3124214910386094
1103	96-97	0.3089266119874239
1103	98-99	0.36423886437029296
1103	100	0.2951698303179242
1104	1	0.27282994050729314
1104	2	0.2559478548432317
1104	3	0.23615689523947836
1104	4	0.24044890298856103
1104	5	0.18639256404091498
1104	6	0.15269258097047356
1104	7	0.14115711282689603
1104	8	0.15072471902509932
1104	9	0.19861451420552356
1104	10-11	0.3050831236909275
1104	12-13	0.3349480656665662
1104	14-15	0.4251979648235107
1104	16-17	0.4594441721822733
1104	18-19	0.437012759937069
1104	20-21	0.3921617485162443
1104	22-23	0.4024045369030844
1104	24-25	0.3960912733635453
1104	26-27	0.4578855915649882
1104	28-29	0.4130169669935526
1104	30-31	0.40278065440028854
1104	32-33	0.42597725800738573
1104	34-35	0.45837874553194524
1104	36-37	0.4017953141391928
1104	38-39	0.4509500016697814
1104	40-41	0.4828711403634003
1104	42-43	0.47427234998858125
1104	44-45	0.47031518859689214
1104	46-47	0.48812094369536396
1104	48-49	0.4560108585452767
1104	50-51	0.4262724030512075
1104	52-53	0.39749996382075636
1104	54-55	0.41111946619807327
1104	56-57	0.3938981989089356
1104	58-59	0.4103664781776999
1104	60-61	0.37362027097954353
1104	62-63	0.3838050831037094
1104	64-65	0.3752833938160691
1104	66-67	0.3628913716357829
1104	68-69	0.4158105708412805
1104	70-71	0.3807211237879713
1104	72-73	0.35998045013262825
1104	74-75	0.3844153024398267
1104	76-77	0.3598508908523037
1104	78-79	0.42034368432073066
1104	80-81	0.3604605821214868
1104	82-83	0.36420358294364874
1104	84-85	0.35913919585934195
1104	86-87	0.353406944135255
1104	88-89	0.33752143134884705
1104	90-91	0.3093357066690352
1104	92-93	0.3055840941175063
1104	94-95	0.24360126414022432
1104	96-97	0.18556316732645683
1104	98-99	0.19197375179285459
1104	100	0.13574226992910354
1105	1	0.18054995073379132
1105	2	0.19692872539074813
1105	3	0.23028585145677383
1105	4	0.13210130486865523
1105	5	0.12550619595245394
1105	6	0.10945932468747088
1105	7	0.1404353949690602
1105	8	0.15926346390727275
1105	9	0.14524127168296275
1105	10-11	0.19441566841890534
1105	12-13	0.22943297519146455
1105	14-15	0.28790142825867093
1105	16-17	0.3069277447650123
1105	18-19	0.2829535362358371
1105	20-21	0.2955046137212207
1105	22-23	0.30126920983973093
1105	24-25	0.34246350962796157
1105	26-27	0.3384647960162255
1105	28-29	0.3226393420050968
1105	30-31	0.3269287147461313
1105	32-33	0.31304710402979197
1105	34-35	0.32288167068384155
1105	36-37	0.29664729954747315
1105	38-39	0.354083502084066
1105	40-41	0.34900157724686665
1105	42-43	0.31695029041443235
1105	44-45	0.303474211545371
1105	46-47	0.34220321407677545
1105	48-49	0.3270455559929175
1105	50-51	0.29910272086467415
1105	52-53	0.2496821461955534
1105	54-55	0.2780163259970294
1105	56-57	0.2693564264625863
1105	58-59	0.2574175854393843
1105	60-61	0.24366040395062782
1105	62-63	0.23444546742315708
1105	64-65	0.1864458203392516
1105	66-67	0.16357754713881434
1105	68-69	0.21172702286028766
1105	70-71	0.20294298729405824
1105	72-73	0.22030158564451696
1105	74-75	0.2221435408067478
1105	76-77	0.2004246622982464
1105	78-79	0.23715083230273137
1105	80-81	0.2423663183630751
1105	82-83	0.2265736057157106
1105	84-85	0.26623157400619846
1105	86-87	0.2662576512787851
1105	88-89	0.2292246996519367
1105	90-91	0.15938004303302478
1105	92-93	0.1593897810558147
1105	94-95	0.1868203470973917
1105	96-97	0.1626402703425569
1105	98-99	0.18638301212351038
1105	100	0.14483726333039115
1106	1	0.18146963446713826
1106	2	0.18145050816212915
1106	3	0.16113301763552812
1106	4	0.16208160589865628
1106	5	0.16577552942026585
1106	6	0.10295804129285813
1106	7	0.08876177286749254
1106	8	0.11509235217037173
1106	9	0.14092943313054462
1106	10-11	0.20334750797093903
1106	12-13	0.210463737818273
1106	14-15	0.27399168364295434
1106	16-17	0.2910196169494341
1106	18-19	0.2928677655364851
1106	20-21	0.2773353183472764
1106	22-23	0.22660163529096167
1106	24-25	0.27948104910728944
1106	26-27	0.31019431636446626
1106	28-29	0.2808635126641832
1106	30-31	0.2895687072342312
1106	32-33	0.27864841882725244
1106	34-35	0.33708364011381775
1106	36-37	0.3026646026858657
1106	38-39	0.2883315366664547
1106	40-41	0.27880342492735366
1106	42-43	0.3377146712530461
1106	44-45	0.35512129367781853
1106	46-47	0.2904041556953558
1106	48-49	0.323964857934385
1106	50-51	0.31255143225422444
1106	52-53	0.2927155338548246
1106	54-55	0.32266236581340735
1106	56-57	0.32923129778969695
1106	58-59	0.3133133160209596
1106	60-61	0.29826366574296515
1106	62-63	0.3046114633644521
1106	64-65	0.2758683823531456
1106	66-67	0.20762107877744995
1106	68-69	0.27180247592828977
1106	70-71	0.26538104771408655
1106	72-73	0.2471508775894229
1106	74-75	0.2590475903827496
1106	76-77	0.2512217886834378
1106	78-79	0.25516112766064225
1106	80-81	0.26792518564558065
1106	82-83	0.25205731631064054
1106	84-85	0.27373200566633216
1106	86-87	0.3336361048797798
1106	88-89	0.28391773283033217
1106	90-91	0.25600146602999985
1106	92-93	0.23501691732439411
1106	94-95	0.2994101102880826
1106	96-97	0.2923524912347091
1106	98-99	0.28526773571979547
1106	100	0.260921882995941
1107	1	0.08110083187914086
1107	2	0.07017163654668934
1107	3	0.08171233451658821
1107	4	0.07971072777714738
1107	5	0.09113568709621944
1107	6	0.043214615888587105
1107	7	0.06695763114134223
1107	8	0.01777933544710919
1107	9	0.05155850701490294
1107	10-11	0.052767560360827304
1107	12-13	0.12812355405673514
1107	14-15	0.10437884892555616
1107	16-17	0.15703764491996708
1107	18-19	0.08358246627475552
1107	20-21	0.10896936632667575
1107	22-23	0.10155065353175985
1107	24-25	0.10183709007222319
1107	26-27	0.11944912055574974
1107	28-29	0.14187160349645467
1107	30-31	0.05506104785519739
1107	32-33	0.06321946771073073
1107	34-35	0.111569464221148
1107	36-37	0.094857400558638
1107	38-39	0.1421070976403911
1107	40-41	0.09729902884740227
1107	42-43	0.10394413872083419
1107	44-45	0.12562263501637716
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2216	70-71	-0.6072956263680496
2216	72-73	-0.6887742407656852
2216	74-75	-0.6207870181081212
2216	76-77	-0.5541288688367949
2216	78-79	-0.5885636839759485
2216	80-81	-0.5964869932189529
2216	82-83	-0.5483093231270573
2216	84-85	-0.6245186606737576
2216	86-87	-0.5529729320555461
2216	88-89	-0.4943941460222625
2216	90-91	-0.41120817314074287
2216	92-93	-0.43498652311217256
2216	94-95	-0.39886914064021184
2216	96-97	-0.3931417517671676
2216	98-99	-0.4143273958780789
2216	100	-0.477914339140348
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	8.0
3	15.0
4	42.0
5	772.0
6	5579.0
7	14225.0
8	26681.0
9	45784.0
10	70922.0
11	90320.0
12	101128.0
13	113061.0
14	121108.0
15	126912.0
16	131865.0
17	136915.0
18	145175.0
19	154058.0
20	165344.0
21	177836.0
22	191541.0
23	217226.0
24	289245.0
25	320759.0
26	306542.0
27	441670.0
28	411407.0
29	571534.0
30	680372.0
31	894503.0
32	1141258.0
33	1590745.0
34	1947204.0
35	3441474.0
36	3829815.0
37	3629986.0
38	1496826.0
39	342860.0
40	2414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.241634076065495	26.147125917317204	27.18898273474249	29.422257271874813
2	15.536141298116727	26.104490466579893	29.76099882986257	28.598369405440817
3	14.021201606922576	27.09086564921713	27.019909848571867	31.868022895288426
4	12.76206721489703	25.569392247698147	27.284500551380457	34.38403998602437
5	12.524863635299951	25.783002105540014	28.11763302170554	33.57450123745449
6	12.480080167885735	23.146115503325653	28.84439604694702	35.52940828184159
7	16.84123118229659	26.381216970489323	24.351970251889522	32.425581595324566
8	14.911271294683928	25.630836187683443	22.073127491567003	37.38476502606562
9	11.848613670228444	24.782693327024607	23.4291335668874	39.93955943585954
10-11	17.59523934962853	37.821618412955544	22.294798920463947	22.288343316951973
12-13	18.50772910909523	39.03911431663262	20.08337773628986	22.36977883798229
14-15	20.82468201220977	36.71114079960689	19.733521412444922	22.73065577573842
16-17	20.13653483247223	35.67143836400855	21.60107099765941	22.590955805859814
18-19	20.269743705232955	33.355875863168905	24.126206194691477	22.248174236906664
20-21	18.731109449313436	33.44699242250635	25.61700335182438	22.20489477635584
22-23	19.77549958235112	35.317591014866565	25.36707249772341	19.53983690505891
24-25	20.85091545617829	36.08367672588381	23.427102397203903	19.638305420734
26-27	23.29111190241503	36.83940461334937	21.923185543707437	17.946297940528165
28-29	22.87293245285874	38.20269445487846	22.653583921654473	16.270789170608328
30-31	23.439508464397914	38.371267630846525	22.31299284523532	15.876231059520244
32-33	24.177001612517028	38.50024685443234	21.520951030579553	15.80180050247108
34-35	24.767794962938368	38.191549875484306	21.16255582669503	15.8780993348823
36-37	25.935519412809576	38.03361691958505	20.28383340590011	15.747030261705264
38-39	26.707719756773052	38.632788814887284	19.817186601262616	14.842304827077044
40-41	27.155776778082192	38.58720932856784	19.76822547173559	14.488788421614379
42-43	27.50971110200181	37.61936450350846	20.52373499194056	14.347189402549171
44-45	27.611079552959744	36.23529947806228	21.468709353133715	14.684911615844257
46-47	27.57758717819528	35.29670555248119	22.66282358558917	14.462883683734356
48-49	27.545839864874118	35.64597651383431	23.437932108484045	13.370251512807524
50-51	28.127253817558913	36.298526197440516	23.34873918841391	12.225480796586668
52-53	28.58762531990427	37.368371853044685	22.62780555296485	11.416197274086192
54-55	28.302392228719352	38.73824928637559	21.911520220903714	11.047838264001339
56-57	27.58628825874809	39.53656694864982	21.44824368748799	11.428901105114091
58-59	26.943621146708285	38.89867723707015	22.205850035413253	11.951851580808308
60-61	26.744922797097804	37.41464139766028	23.60375489608872	12.236680909153199
62-63	26.4790797311184	35.84402601313475	24.72163599952997	12.955258256216881
64-65	25.621660824016757	35.19190361913426	25.213941402476003	13.972494154372987
66-67	24.45487040510646	34.18702749566531	26.036306062713383	15.32179603651485
68-69	24.20736389698054	33.16724438224794	25.80520214426209	16.820189576509435
70-71	24.4786499774874	32.34494754608622	25.89071728171471	17.285685194711668
72-73	25.32446686637896	32.46603675216729	25.68272316115618	16.526773220297574
74-75	25.13271337703731	33.11984321047132	25.202346836714824	16.545096575776547
76-77	23.578274961339428	34.435882160367306	24.89413779495198	17.09170508334128
78-79	21.94001527594331	36.15832168896196	24.210226435554535	17.69143659954019
80-81	20.13908926076673	38.80749470009103	23.80089920551537	17.252516833626867
82-83	18.22158105251812	41.53276481998852	24.26213892778409	15.98351519970927
84-85	16.42764398073333	44.38511152760065	24.564255468657382	14.622989023008637
86-87	15.042135254547926	47.249503384992245	24.204359086796632	13.504002273663197
88-89	14.182665497222033	50.19558244750469	23.24165944321727	12.380092612055998
90-91	13.87215624442665	53.08740610672964	21.520367090466504	11.5200705583772
92-93	13.915486663429192	55.10766701827035	19.800464380434065	11.176381937866406
94-95	14.27981771557062	55.881684306895174	18.63538360313878	11.203114374395424
96-97	14.823092634950008	55.463623613676205	18.202629326803173	11.510654424570616
98-99	15.719373982493062	54.05165871602091	18.057355043342884	12.171612258143135
100	17.088460408023128	52.654312009999835	17.66695193738267	12.590275644594366
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	920591.0
1	779398.0
2	400982.0
3	116345.5
4	66630.0
5	60274.0
6	49906.5
7	38680.5
8	31328.0
9	29020.5
10	31269.5
11	37197.5
12	47296.5
13	63228.0
14	85531.5
15	113032.5
16	144319.0
17	176940.0
18	209219.5
19	239709.5
20	266145.5
21	287470.0
22	303291.5
23	314933.0
24	323593.5
25	328402.5
26	328409.0
27	322414.0
28	311048.5
29	295291.5
30	276923.0
31	256902.0
32	235885.0
33	217408.0
34	201489.0
35	187803.5
36	178273.5
37	173989.5
38	176559.5
39	188760.5
40	214446.5
41	259709.0
42	332998.5
43	445620.0
44	605406.5
45	809364.5
46	1031696.5
47	1220389.5
48	1322135.0
49	1315456.0
50	1221122.5
51	1086846.0
52	958712.5
53	858344.0
54	772283.0
55	674089.0
56	555471.0
57	422430.0
58	293454.0
59	188562.5
60	114494.5
61	67815.5
62	41094.5
63	27372.5
64	20277.0
65	16164.5
66	13686.0
67	11816.5
68	10258.5
69	8967.5
70	8011.5
71	7314.5
72	6726.5
73	6079.0
74	5538.0
75	5055.5
76	4571.0
77	4194.0
78	3913.5
79	3713.0
80	3540.5
81	3395.5
82	3222.5
83	3009.0
84	2891.5
85	2747.0
86	2575.0
87	2522.5
88	2363.5
89	2169.5
90	2034.5
91	1918.0
92	1824.5
93	1702.0
94	1567.5
95	1404.0
96	1247.0
97	1076.0
98	908.5
99	767.0
100	698.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.6684397760834035E-4
2	4.2780507079061633E-4
3	7.272686203440478E-5
4	7.058783668045168E-4
5	5.604246427357073E-4
6	7.957174316705462E-4
7	8.64166242997045E-4
8	0.0012149664010453502
9	4.3636117220642866E-4
10-11	3.8716358906550774E-4
12-13	7.999954823784525E-4
14-15	0.0024427669542144187
16-17	0.0012449127560006934
18-19	0.0034053283634933058
20-21	0.0036213699242425667
22-23	0.0035614772143318808
24-25	0.004832058274580011
26-27	0.006909051893268453
28-29	0.008793533230101118
30-31	0.009309038340403811
32-33	0.008750752723022056
34-35	0.011024536674274181
36-37	0.010299407079284086
38-39	0.00882989666111832
40-41	0.007202098366760025
42-43	0.004203184820517805
44-45	0.005024570556435788
46-47	0.007266269127378619
48-49	0.005869485571247255
50-51	0.003858801738531359
52-53	0.004072704273926668
54-55	0.0041347360091913065
56-57	0.006727234738182441
58-59	0.00778819131374317
60-61	0.007510118017729269
62-63	0.008956099157001553
64-65	0.009918660566280438
66-67	0.008838452762534133
68-69	0.011191380651882523
70-71	0.011390310009800158
72-73	0.013591367099017879
74-75	0.017897225136525433
76-77	0.018913262179653147
78-79	0.018817006038725256
80-81	0.023062971366322123
82-83	0.021379558412761047
84-85	0.021092929015331333
86-87	0.021030897280066695
88-89	0.02166618781019076
90-91	0.02130469252537269
92-93	0.02168543903837634
94-95	0.018836257266910832
96-97	0.0214137828184243
98-99	0.018327169232670002
100	0.018605242528683903
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.3375132E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.9156701402924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.21062356424478	84.58421445265988
2	3.401113451227252	5.980223365756132
3	0.2567092851295877	0.677063065002093
4	0.052485475477702694	0.18457182997016464
5	0.020617565156513317	0.09063035286980055
6	0.011362275168446536	0.059935322145142855
7	0.008083781504609758	0.04974837477718277
8	0.005216878705779272	0.03669163099673648
9	0.002741069535173398	0.02168846685773181
>10	0.025323334981498195	0.44359042508815005
>50	0.0032134173652263753	0.19276987313458938
>100	0.0018149295114377297	0.3199908645018589
>500	3.1544447584599093E-4	0.19920512823264264
>1k	2.1417918735740674E-4	0.42403280771918994
>5k	5.34950476047575E-5	0.320359141082768
>10k+	1.1185328135432648E-4	6.415284899205897
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	659631	2.82193486650685	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	144943	0.620073503756043	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	123633	0.5289082431705626	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	113511	0.4856058139051364	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54064	0.2312885334722388	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42311	0.18100860350221765	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39112	0.16732311928762583	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37992	0.16253170249477092	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33800	0.1445981139272283	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28991	0.12402496807290757	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28457	0.12174048899488568	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27500	0.11764639446741948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.00220747416527958	0.0	0.0	4.278050707906163E-6	0.0
2	0.005647026934436135	0.0	0.0	2.5668304247436976E-5	0.0
3	0.009848072729599987	0.0	0.0	5.133660849487395E-5	4.278050707906163E-6
4	0.016906856397645154	0.0	0.0	5.561465920278012E-5	4.278050707906163E-6
5	0.02909930091517772	0.0	0.0	5.561465920278012E-5	4.278050707906163E-6
6	0.0495654955018008	0.0	0.0	7.272686203440476E-5	4.278050707906163E-6
7	0.07541775592967774	0.0	0.0	8.128296345021709E-5	4.278050707906163E-6
8	0.1665274018559553	0.0	0.0	8.128296345021709E-5	4.278050707906163E-6
9	0.498803600339027	0.0	0.0	8.128296345021709E-5	4.278050707906163E-6
10-11	1.242236835282898	0.0	0.0	8.128296345021709E-5	4.278050707906163E-6
12-13	2.298575255104442	0.0	0.0	8.556101415812326E-5	4.278050707906163E-6
14-15	3.5981422479239904	0.0	0.0	8.556101415812326E-5	4.278050707906163E-6
16-17	5.026927762375845	0.0	4.278050707906163E-6	9.625614092788866E-5	4.278050707906163E-6
18-19	6.5807072233859465	0.0	4.278050707906163E-6	1.155073691134664E-4	4.278050707906163E-6
20-21	8.621976979638019	0.0	4.278050707906163E-6	1.1978541982137256E-4	4.278050707906163E-6
22-23	11.02743719265414	0.0	4.278050707906163E-6	1.2192444517532565E-4	4.278050707906163E-6
24-25	13.66711640387742	0.0	4.278050707906163E-6	1.262024958832318E-4	4.278050707906163E-6
26-27	16.729105529757007	0.0	4.278050707906163E-6	1.283415212371849E-4	4.278050707906163E-6
28-29	20.68728210818232	0.0	4.278050707906163E-6	1.283415212371849E-4	4.278050707906163E-6
30-31	25.652693640403825	0.0	4.278050707906163E-6	1.283415212371849E-4	4.278050707906163E-6
32-33	30.999279490699777	0.0	4.278050707906163E-6	1.3048054659113798E-4	4.278050707906163E-6
34-35	35.425389255555864	0.0	4.278050707906163E-6	1.3903664800695028E-4	4.278050707906163E-6
36-37	38.5272562311092	0.0	4.278050707906163E-6	1.5400982548462186E-4	4.278050707906163E-6
38-39	40.60943056920492	0.0	4.278050707906163E-6	1.83956180439965E-4	4.278050707906163E-6
40-41	42.173019600488246	0.0	8.556101415812326E-6	2.053464339794958E-4	4.278050707906163E-6
42-43	43.33488897517242	0.0	1.2834152123718488E-5	2.4812694105855746E-4	6.417076061859245E-6
44-45	44.234477478030925	0.0	1.2834152123718488E-5	2.7807329601390056E-4	8.556101415812326E-6
46-47	44.96497174860873	0.0	1.2834152123718488E-5	2.8876842278366594E-4	8.556101415812326E-6
48-49	45.61489535117919	0.0	1.2834152123718488E-5	3.101586763231968E-4	8.556101415812326E-6
50-51	46.27101998825076	0.0	1.2834152123718488E-5	3.550782087562115E-4	8.556101415812326E-6
52-53	46.98539242473582	0.0	1.2834152123718488E-5	4.021367665431793E-4	8.556101415812326E-6
54-55	47.750934625738154	0.0	1.2834152123718488E-5	4.876977807013026E-4	8.556101415812326E-6
56-57	48.56850220139933	0.0	1.2834152123718488E-5	5.155051103026926E-4	8.556101415812326E-6
58-59	49.407663238008666	0.0	1.2834152123718488E-5	5.390343891961765E-4	8.556101415812326E-6
60-61	50.2410874086187	0.0	1.7112202831624653E-5	5.625636680896604E-4	8.556101415812326E-6
62-63	51.002203966163705	0.0	2.1390253539530814E-5	5.989270991068629E-4	8.556101415812326E-6
64-65	51.68474770538194	0.0	2.1390253539530814E-5	6.417076061859244E-4	8.556101415812326E-6
66-67	52.27476148583888	0.0	2.1390253539530814E-5	7.165734935742822E-4	8.556101415812326E-6
68-69	52.770538365302066	0.0	2.1390253539530814E-5	7.807442541928747E-4	8.556101415812326E-6
70-71	53.20592200292174	0.0	2.1390253539530814E-5	8.064125584403116E-4	8.556101415812326E-6
72-73	53.60586840750247	0.0	2.1390253539530814E-5	8.235247612719363E-4	8.556101415812326E-6
74-75	53.97857432420061	0.0	2.1390253539530814E-5	8.278028119798425E-4	8.556101415812326E-6
76-77	54.33486749935787	0.0	2.1390253539530814E-5	8.812784458286695E-4	8.556101415812326E-6
78-79	54.676431345927796	0.0	2.1390253539530814E-5	9.818126374644643E-4	8.556101415812326E-6
80-81	55.00528510384454	0.0	2.1390253539530814E-5	0.0011336834375951332	8.556101415812326E-6
82-83	55.32460308673337	0.0	2.1390253539530814E-5	0.00115935174184257	8.556101415812326E-6
84-85	55.643305030320256	0.0	2.1390253539530814E-5	0.0011999932235676786	8.556101415812326E-6
86-87	55.96278771816134	0.0	2.1390253539530814E-5	0.0012192444517532563	8.556101415812326E-6
88	56.204837688189315	0.0	2.1390253539530814E-5	0.0012278005531690687	8.556101415812326E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	11615	0.0	45.03258	9
TACCCGA	17755	0.0	40.416447	6
CTACCCG	17425	0.0	38.32305	5
ACCCGAC	19270	0.0	38.019547	7
GCTTGCT	2235	0.0	37.216732	7
CTGCTGA	10975	0.0	36.910812	9
GCCTGCT	2330	0.0	36.909462	7
CTTGCTG	7405	0.0	36.364193	8
CCTGCTG	7780	0.0	35.8799	8
CCCGACG	21690	0.0	35.684402	8
GTTGCTG	3535	0.0	32.03849	8
ACTGGCC	1420	0.0	30.115753	3
ATTGCTG	2205	0.0	29.62447	8
GTACACG	9770	0.0	29.437523	8
CTGCACG	17850	0.0	29.38113	7
ACTGCTG	1645	0.0	28.853592	8
CCTGCAC	14450	0.0	28.553965	6
CGTACAC	7575	0.0	28.53762	7
TCGACAC	3255	0.0	28.4416	5
CCCTGCA	11700	0.0	28.356829	5
>>END_MODULE