FastQCFastQC Report
Thu 26 May 2016
SRR1248495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27893745
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8694713.117082342295737No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1907740.6839311107203425No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640570.5881497805332343No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1481490.5311190734696972No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA718680.25764916112913483No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA584720.2096240572931315No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA525080.18824291969400309No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA516220.18506658033906884No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440460.15790636933118876No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA405140.1452440323090356No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA359630.12892854652539484No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA303700.108877456218231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTGA153650.045.1436169
GCTTGCT31800.041.673197
TACCCGA216850.041.00096
CTACCCG218050.038.986425
ACCCGAC234850.038.7591137
CTGCTGA141500.038.1265959
CCTGCTG100550.036.7348568
CTTGCTG101900.036.663248
GTTGCTG40000.036.4199688
CCCGACG268450.035.2035568
GCCTGCT29850.032.273377
ATTGCTG26850.030.803948
CGTACAC95850.030.544347
TCGCGAC16650.030.481846
GTACACG131800.030.1642728
ACTTGCT24500.030.1139957
CCCTGCA138550.029.542185
CTCCACG324300.029.3725437
TTTGCTG42300.029.1070928
CTGCACG222800.029.0016237
Produced by FastQC (version 0.11.5)