##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248495_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27893745 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.71348106896367 33.0 31.0 34.0 26.0 34.0 2 30.959364975911267 34.0 31.0 34.0 26.0 34.0 3 31.104708492889714 34.0 31.0 34.0 27.0 34.0 4 34.444930108882836 37.0 35.0 37.0 30.0 37.0 5 34.38332697886211 37.0 35.0 37.0 30.0 37.0 6 34.3309100660381 37.0 35.0 37.0 30.0 37.0 7 34.35812362233899 37.0 35.0 37.0 30.0 37.0 8 34.32184824949106 37.0 35.0 37.0 30.0 37.0 9 35.8190948902702 39.0 37.0 39.0 30.0 39.0 10-11 35.97565404000072 39.0 37.0 39.0 30.0 39.0 12-13 35.97480119646896 39.0 37.0 39.0 30.0 39.0 14-15 37.063837017940756 40.0 37.0 41.0 30.0 41.0 16-17 36.881486530403144 40.0 37.0 41.0 30.0 41.0 18-19 36.59613103224397 39.0 36.0 41.0 30.0 41.0 20-21 36.423199699430825 39.0 35.0 41.0 29.0 41.0 22-23 36.30367162960728 39.0 35.0 41.0 28.0 41.0 24-25 36.16908009304595 39.0 35.0 41.0 27.0 41.0 26-27 36.0057556093669 39.0 35.0 41.0 26.5 41.0 28-29 35.865296933057934 39.0 35.0 41.0 25.0 41.0 30-31 35.795517400047935 39.0 35.0 41.0 25.0 41.0 32-33 35.7015781315847 39.0 35.0 41.0 25.0 41.0 34-35 35.57906439239335 39.0 35.0 40.0 25.0 41.0 36-37 35.46795389073787 39.0 35.0 40.0 25.0 41.0 38-39 35.28751904772916 38.0 34.5 40.0 25.0 41.0 40-41 35.06436444801514 38.0 34.0 40.0 25.0 41.0 42-43 34.782165159249864 38.0 33.0 40.0 25.0 41.0 44-45 34.553557078836135 38.0 33.0 40.0 24.5 41.0 46-47 34.37197368083777 37.0 33.0 40.0 24.5 41.0 48-49 34.24630902017638 37.0 33.0 39.5 25.0 41.0 50-51 33.94889006477976 36.0 33.0 39.0 25.0 41.0 52-53 33.57403900049994 35.0 32.0 39.0 25.0 40.5 54-55 33.382634798590146 35.0 32.0 38.5 25.0 40.0 56-57 33.24358435914576 35.0 32.0 37.0 25.0 40.0 58-59 32.9593970081823 35.0 32.0 37.0 25.0 39.5 60-61 32.68143859851017 35.0 32.0 36.0 25.0 39.0 62-63 32.429324549285155 35.0 32.0 36.0 25.0 39.0 64-65 32.281295053783566 35.0 31.0 35.0 24.5 38.0 66-67 32.1582847516531 35.0 31.0 35.0 24.0 37.0 68-69 32.013055023626265 35.0 31.0 35.0 24.0 37.0 70-71 31.849633475175168 35.0 31.0 35.0 24.0 36.0 72-73 31.68020880666974 35.0 31.0 35.0 23.0 36.0 74-75 31.5002083262753 35.0 31.0 35.0 23.0 36.0 76-77 31.292024842845592 35.0 31.0 35.0 22.0 36.0 78-79 31.109673243947704 35.0 30.0 35.0 20.0 35.0 80-81 30.891733953257262 35.0 30.0 35.0 20.0 35.0 82-83 30.69824915944417 35.0 30.0 35.0 19.5 35.0 84-85 30.50878713130847 35.0 30.0 35.0 17.5 35.0 86-87 30.250010369708335 35.0 29.0 35.0 16.0 35.5 88-89 29.962950923226693 34.5 29.0 35.0 12.5 36.0 90-91 29.529984500109254 34.0 28.0 35.0 10.0 35.0 92-93 28.973374424983092 34.0 26.5 35.0 9.0 35.0 94-95 28.2313006195475 34.0 25.0 35.0 8.0 35.0 96-97 27.273117145080377 33.0 22.0 35.0 7.0 35.0 98-99 26.022875791687348 33.0 17.0 35.0 7.0 35.0 100 24.256207332504115 29.0 10.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5396923998236609 1101 2 0.48263059123863883 1101 3 0.45875277244607204 1101 4 0.4274786718419392 1101 5 0.3834566066365639 1101 6 0.37148282189809834 1101 7 0.33003320796179025 1101 8 0.3532821624729223 1101 9 0.403708074396917 1101 10-11 0.4819404403857206 1101 12-13 0.48787388870339043 1101 14-15 0.6435461462104257 1101 16-17 0.6525018858970455 1101 18-19 0.6815936246512635 1101 20-21 0.6889229936653294 1101 22-23 0.6477069206000863 1101 24-25 0.6692345270582578 1101 26-27 0.6690319034368954 1101 28-29 0.6558667913432075 1101 30-31 0.6503341377374099 1101 32-33 0.6614992627204757 1101 34-35 0.6610549845131075 1101 36-37 0.6593924362938992 1101 38-39 0.6432701173368045 1101 40-41 0.6548537223823487 1101 42-43 0.6769280746227295 1101 44-45 0.6980564943455718 1101 46-47 0.7264847188027659 1101 48-49 0.712086575349204 1101 50-51 0.6603833615868524 1101 52-53 0.6472681118331067 1101 54-55 0.6099336186712065 1101 56-57 0.5770636241092362 1101 58-59 0.5911860334547612 1101 60-61 0.542018052662641 1101 62-63 0.5891220754786701 1101 64-65 0.5596242592791327 1101 66-67 0.5547597375002624 1101 68-69 0.5810389582234521 1101 70-71 0.5700039661843128 1101 72-73 0.5800927576853887 1101 74-75 0.620530439720131 1101 76-77 0.6205612463097054 1101 78-79 0.6034903175871875 1101 80-81 0.5334476767866718 1101 82-83 0.5469230547028019 1101 84-85 0.5139339942263987 1101 86-87 0.5090199831937952 1101 88-89 0.4744559360265299 1101 90-91 0.46787006976148504 1101 92-93 0.40882481649489577 1101 94-95 0.41814026583849895 1101 96-97 0.4462805547469024 1101 98-99 0.3788770479368111 1101 100 0.393542601759183 1102 1 0.36886534600764165 1102 2 0.37175592590116935 1102 3 0.3620544213527097 1102 4 0.31380626930868516 1102 5 0.32540342637113184 1102 6 0.28653092752076503 1102 7 0.28029569185318337 1102 8 0.3046665613302153 1102 9 0.2603655067561377 1102 10-11 0.39202804242183475 1102 12-13 0.4247895226821754 1102 14-15 0.5984755610539878 1102 16-17 0.6139361300743502 1102 18-19 0.6252897158743025 1102 20-21 0.5888108929243714 1102 22-23 0.5736168629264853 1102 24-25 0.576057224423316 1102 26-27 0.6165336037570128 1102 28-29 0.6210691112292608 1102 30-31 0.6232596249928619 1102 32-33 0.6332784742077777 1102 34-35 0.6728625013869589 1102 36-37 0.6215605461923275 1102 38-39 0.6432203426606691 1102 40-41 0.6617906074849031 1102 42-43 0.6451826162339387 1102 44-45 0.6639475341966588 1102 46-47 0.656949961125207 1102 48-49 0.666933514003972 1102 50-51 0.624434489200425 1102 52-53 0.5694783136104746 1102 54-55 0.5760646795912052 1102 56-57 0.5752107250091782 1102 58-59 0.5752325095398021 1102 60-61 0.5271692748163161 1102 62-63 0.5245948921587384 1102 64-65 0.488415236003064 1102 66-67 0.4933778957766535 1102 68-69 0.49705143814419017 1102 70-71 0.5212500078928421 1102 72-73 0.4991582018425298 1102 74-75 0.5583581681591276 1102 76-77 0.5228210297792124 1102 78-79 0.5599281840476031 1102 80-81 0.5014133049354186 1102 82-83 0.5048158281074251 1102 84-85 0.4550427515866424 1102 86-87 0.46187632386526545 1102 88-89 0.40270903132233116 1102 90-91 0.3344918548717821 1102 92-93 0.3371475535251385 1102 94-95 0.36282737177478097 1102 96-97 0.4044164816299265 1102 98-99 0.3072192281161499 1102 100 0.19828406889305938 1103 1 0.392714173433351 1103 2 0.35619806065593806 1103 3 0.3571046593886429 1103 4 0.30985415068029454 1103 5 0.2863779559429318 1103 6 0.25467502093385974 1103 7 0.26362348130824387 1103 8 0.2378277172322285 1103 9 0.34013565983091354 1103 10-11 0.44433695337515644 1103 12-13 0.47255305539705716 1103 14-15 0.571101573546926 1103 16-17 0.584987762221779 1103 18-19 0.5960012437617692 1103 20-21 0.5913473813691823 1103 22-23 0.5650824253104787 1103 24-25 0.5508577443049631 1103 26-27 0.5834797795394664 1103 28-29 0.5910473624796353 1103 30-31 0.5923182144855232 1103 32-33 0.5799278722679091 1103 34-35 0.6260330473083116 1103 36-37 0.6085690512786712 1103 38-39 0.6174934080089542 1103 40-41 0.6087497467975922 1103 42-43 0.6290509057978539 1103 44-45 0.6280815022808355 1103 46-47 0.6246167231687636 1103 48-49 0.6082310213090096 1103 50-51 0.5667281353382805 1103 52-53 0.543740328469049 1103 54-55 0.5331257133863971 1103 56-57 0.5550183613858266 1103 58-59 0.5137547462563958 1103 60-61 0.4914403906724658 1103 62-63 0.4805749956115193 1103 64-65 0.458059054539774 1103 66-67 0.43328590480970064 1103 68-69 0.43234167811380075 1103 70-71 0.43045743605444997 1103 72-73 0.4260995085966677 1103 74-75 0.46780294747616225 1103 76-77 0.4473940845521618 1103 78-79 0.5060508445242 1103 80-81 0.47141685614443674 1103 82-83 0.44852211070838877 1103 84-85 0.40342188974574356 1103 86-87 0.4416135688699043 1103 88-89 0.40233216100147473 1103 90-91 0.397591507891061 1103 92-93 0.3231176517646759 1103 94-95 0.33265491944233005 1103 96-97 0.3255481691964057 1103 98-99 0.3267750087070951 1103 100 0.2517883259199003 1104 1 0.24206955163988653 1104 2 0.2344573872323572 1104 3 0.2174831101379766 1104 4 0.2100234011016866 1104 5 0.13257769335923086 1104 6 0.14412941412123814 1104 7 0.12034546415161884 1104 8 0.16052859993965285 1104 9 0.15890768417639833 1104 10-11 0.2869819982844106 1104 12-13 0.3347708358504846 1104 14-15 0.4027981737215711 1104 16-17 0.4373814971646297 1104 18-19 0.45981473341998935 1104 20-21 0.42350130460779667 1104 22-23 0.41014719621853857 1104 24-25 0.3966956920544433 1104 26-27 0.40053343114814766 1104 28-29 0.3780828230530844 1104 30-31 0.38640534217863376 1104 32-33 0.4346807036131466 1104 34-35 0.4530133895378583 1104 36-37 0.40510049511011914 1104 38-39 0.4590710795160575 1104 40-41 0.46061551251035127 1104 42-43 0.46460145552151033 1104 44-45 0.47534279893664433 1104 46-47 0.4614000109098484 1104 48-49 0.4199510174386063 1104 50-51 0.39774906081319017 1104 52-53 0.3719839379472134 1104 54-55 0.41041143281106685 1104 56-57 0.38281327782867436 1104 58-59 0.4136240509859732 1104 60-61 0.3793148975151368 1104 62-63 0.39440962154954917 1104 64-65 0.3740077797657051 1104 66-67 0.35179050368672904 1104 68-69 0.3683444182009552 1104 70-71 0.3800886496776421 1104 72-73 0.3599404271926083 1104 74-75 0.36315251549041605 1104 76-77 0.34265813060489947 1104 78-79 0.36859192692091725 1104 80-81 0.35842616958619544 1104 82-83 0.3059937009066829 1104 84-85 0.2831099477870147 1104 86-87 0.2999630801698032 1104 88-89 0.2633856802880672 1104 90-91 0.24731371776299582 1104 92-93 0.20838830194221458 1104 94-95 0.231695182835157 1104 96-97 0.23663414401707783 1104 98-99 0.2595336093089671 1104 100 0.18859322604016882 1105 1 0.1542674302904956 1105 2 0.19046023504929366 1105 3 0.20142220002142253 1105 4 0.11526278148419067 1105 5 0.13501381279990454 1105 6 0.12355786065106855 1105 7 0.16278472620277284 1105 8 0.13703448080580216 1105 9 0.12675195945122653 1105 10-11 0.17417986399980379 1105 12-13 0.23012654779566333 1105 14-15 0.27807897266253434 1105 16-17 0.31061413998168774 1105 18-19 0.3014903437099008 1105 20-21 0.29731489994563987 1105 22-23 0.3164905105362408 1105 24-25 0.3340329162788933 1105 26-27 0.3362851472631192 1105 28-29 0.318387956367296 1105 30-31 0.3458512357264709 1105 32-33 0.33236315029353847 1105 34-35 0.33903853475504775 1105 36-37 0.31924936216637434 1105 38-39 0.35996336811002294 1105 40-41 0.3738884275024503 1105 42-43 0.3694449449173476 1105 44-45 0.39943526522166195 1105 46-47 0.39905036338152655 1105 48-49 0.359726014870958 1105 50-51 0.3048878096855674 1105 52-53 0.28546978016035496 1105 54-55 0.3158122779850885 1105 56-57 0.30211121030271215 1105 58-59 0.27015247114090357 1105 60-61 0.23109160427148367 1105 62-63 0.24985931106558468 1105 64-65 0.2344986465984391 1105 66-67 0.18209308197259588 1105 68-69 0.20367032256003625 1105 70-71 0.21563543124092277 1105 72-73 0.21645314496665335 1105 74-75 0.2685195525397148 1105 76-77 0.2432361093197457 1105 78-79 0.2852455279424859 1105 80-81 0.2667890173074099 1105 82-83 0.23193941808286667 1105 84-85 0.21584542108782756 1105 86-87 0.21075129170040796 1105 88-89 0.21363661796180367 1105 90-91 0.13463480336643485 1105 92-93 0.08911215851448517 1105 94-95 0.14985358818961103 1105 96-97 0.14586452976847397 1105 98-99 0.14113427716705118 1105 100 0.11361576371204407 1106 1 0.1602412316050028 1106 2 0.15027243446064986 1106 3 0.18255716477793626 1106 4 0.15501281200215544 1106 5 0.14377527956325054 1106 6 0.12083404030863676 1106 7 0.10198634360509118 1106 8 0.09411656273217517 1106 9 0.12403666134009228 1106 10-11 0.16880991942970525 1106 12-13 0.22304722906184082 1106 14-15 0.2698708729883208 1106 16-17 0.28659241331262564 1106 18-19 0.2748140019384735 1106 20-21 0.27392951699294343 1106 22-23 0.26100110483116623 1106 24-25 0.2777590481288428 1106 26-27 0.29131892310190466 1106 28-29 0.2631304191733719 1106 30-31 0.2918028089270521 1106 32-33 0.27977001822355163 1106 34-35 0.32782501251190155 1106 36-37 0.28104512059560705 1106 38-39 0.2948580345116767 1106 40-41 0.2776370594694413 1106 42-43 0.3059891211271051 1106 44-45 0.31333969715230836 1106 46-47 0.30704603096220495 1106 48-49 0.30975158383974133 1106 50-51 0.3165117270750599 1106 52-53 0.30081735066692517 1106 54-55 0.306919788294465 1106 56-57 0.3001567778747827 1106 58-59 0.2987349475726262 1106 60-61 0.2726198385289109 1106 62-63 0.2758237166646609 1106 64-65 0.26872995655882903 1106 66-67 0.20977773048539916 1106 68-69 0.2232090017295576 1106 70-71 0.2370037807783767 1106 72-73 0.22665608496318868 1106 74-75 0.23662149283037692 1106 76-77 0.22227671805867644 1106 78-79 0.21718011556693284 1106 80-81 0.2425392282647607 1106 82-83 0.24118008774163968 1106 84-85 0.19993531107255436 1106 86-87 0.23173341096105204 1106 88-89 0.2076421935361772 1106 90-91 0.22835647852165053 1106 92-93 0.2139965369026271 1106 94-95 0.1861534489176222 1106 96-97 0.2408002140179768 1106 98-99 0.21704125856859946 1106 100 0.21774447618929926 1107 1 0.1315875832280753 1107 2 0.10373294156229207 1107 3 0.1260046008687432 1107 4 0.11188708700453276 1107 5 0.11598359235541977 1107 6 0.05882003507433353 1107 7 0.024213287071361833 1107 8 0.05078517263084592 1107 9 0.07187566617239582 1107 10-11 0.0797020695310593 1107 12-13 0.14908863132878736 1107 14-15 0.18329745598363445 1107 16-17 0.17707096388677002 1107 18-19 0.16514678648697867 1107 20-21 0.1395832759215878 1107 22-23 0.1414101425337151 1107 24-25 0.14992835452913766 1107 26-27 0.20169054247273976 1107 28-29 0.18211045389892178 1107 30-31 0.1796773804841294 1107 32-33 0.1344804347016435 1107 34-35 0.17779611202407608 1107 36-37 0.18913056022171304 1107 38-39 0.21079030942057386 1107 40-41 0.17713881605132542 1107 42-43 0.15844844362702304 1107 44-45 0.18852762971003045 1107 46-47 0.20913473521689951 1107 48-49 0.17831632385701823 1107 50-51 0.18850519670264276 1107 52-53 0.2001872082451044 1107 54-55 0.19147640324060688 1107 56-57 0.18357535582720885 1107 58-59 0.174381998296397 1107 60-61 0.15942825063594768 1107 62-63 0.1416603885091945 1107 64-65 0.1629215438560223 1107 66-67 0.13506177559212773 1107 68-69 0.14046998679765466 1107 70-71 0.13127719729852672 1107 72-73 0.14854522630818323 1107 74-75 0.14781192242640273 1107 76-77 0.13395779311872857 1107 78-79 0.1974072120723065 1107 80-81 0.19441974479169133 1107 82-83 0.1957145946634853 1107 84-85 0.20116445542744188 1107 86-87 0.20180339434289962 1107 88-89 0.15655018023439027 1107 90-91 0.11343403918177941 1107 92-93 0.09343477565666092 1107 94-95 0.10407747925540534 1107 96-97 0.08455320414043399 1107 98-99 0.0896724342347035 1107 100 0.04705148992090713 1108 1 0.0363280666520005 1108 2 0.053606577868581695 1108 3 0.08671253859648331 1108 4 0.05779084063914297 1108 5 0.09854713486922861 1108 6 0.05186310407253103 1108 7 0.003339550610391484 1108 8 0.048856072949220675 1108 9 0.03064366292567655 1108 10-11 0.026383678122591903 1108 12-13 0.07320344098886267 1108 14-15 0.10657744863510032 1108 16-17 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-0.47551855886463557 2215 10-11 -0.4985801299074595 2215 12-13 -0.5799830783273592 2215 14-15 -0.7604102239307196 2215 16-17 -0.7435412220984077 2215 18-19 -0.7254427013549858 2215 20-21 -0.7561974271656666 2215 22-23 -0.7494911324218876 2215 24-25 -0.7972694611578746 2215 26-27 -0.7666857680956198 2215 28-29 -0.7475372847354791 2215 30-31 -0.7397949749619599 2215 32-33 -0.7874062886435382 2215 34-35 -0.7722538630685207 2215 36-37 -0.8326112896699129 2215 38-39 -0.7984969010015703 2215 40-41 -0.8745319832602121 2215 42-43 -0.8218534246859761 2215 44-45 -0.8353504451186993 2215 46-47 -0.9011962096229809 2215 48-49 -0.764917963644443 2215 50-51 -0.7813359518208358 2215 52-53 -0.7713530594577733 2215 54-55 -0.7327391958655909 2215 56-57 -0.6843220777533716 2215 58-59 -0.6581127244279514 2215 60-61 -0.6286642461322742 2215 62-63 -0.6444330288325375 2215 64-65 -0.6807411646672534 2215 66-67 -0.721781425811276 2215 68-69 -0.6460016765516912 2215 70-71 -0.5938673783956894 2215 72-73 -0.5755988199724023 2215 74-75 -0.6413768828329793 2215 76-77 -0.5916126332778155 2215 78-79 -0.6263736414126129 2215 80-81 -0.6154223107035577 2215 82-83 -0.5279904117506007 2215 84-85 -0.5523931714008263 2215 86-87 -0.4848290604655645 2215 88-89 -0.47842134891271826 2215 90-91 -0.4176757682281895 2215 92-93 -0.42531719679993074 2215 94-95 -0.38006450440040496 2215 96-97 -0.3780876232006847 2215 98-99 -0.3131866902271483 2215 100 -0.38354412125432447 2216 1 -0.2655130328624118 2216 2 -0.3271312664939785 2216 3 -0.3589810168099703 2216 4 -0.2614881669076823 2216 5 -0.32840156131904763 2216 6 -0.1901482139570021 2216 7 -0.29733349775863616 2216 8 -0.2853947772534937 2216 9 -0.2596602486051367 2216 10-11 -0.36742373677482476 2216 12-13 -0.5003924621629139 2216 14-15 -0.6212389942913674 2216 16-17 -0.7442801478362142 2216 18-19 -0.6841950067918177 2216 20-21 -0.6513639001517078 2216 22-23 -0.6354746950570132 2216 24-25 -0.6606841424677583 2216 26-27 -0.7087818520415752 2216 28-29 -0.6121137950015267 2216 30-31 -0.6277799828294377 2216 32-33 -0.7122308484507087 2216 34-35 -0.636575798470183 2216 36-37 -0.6496348849753346 2216 38-39 -0.6779830982445674 2216 40-41 -0.6708715089489345 2216 42-43 -0.7289941880972606 2216 44-45 -0.8766222689414391 2216 46-47 -0.7786303609694372 2216 48-49 -0.6629995834304054 2216 50-51 -0.6102263903749474 2216 52-53 -0.6701980637582992 2216 54-55 -0.6463834034542657 2216 56-57 -0.5864701608144074 2216 58-59 -0.5458338842879016 2216 60-61 -0.5060473858782046 2216 62-63 -0.45360345804856195 2216 64-65 -0.4242635826909691 2216 66-67 -0.47646715429344155 2216 68-69 -0.41295308215354964 2216 70-71 -0.5489647249012712 2216 72-73 -0.6453406483973865 2216 74-75 -0.6191542796945413 2216 76-77 -0.5520859761654933 2216 78-79 -0.5565071687017493 2216 80-81 -0.5681352394800214 2216 82-83 -0.5545938154605636 2216 84-85 -0.5751341288577798 2216 86-87 -0.5145046840905856 2216 88-89 -0.48082222540995545 2216 90-91 -0.4043182357761097 2216 92-93 -0.3857083890986459 2216 94-95 -0.38869570354032845 2216 96-97 -0.3735201461600859 2216 98-99 -0.33817274647578444 2216 100 -0.42785564648658436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 23.0 4 55.0 5 909.0 6 6247.0 7 16797.0 8 33342.0 9 56873.0 10 87296.0 11 111895.0 12 125208.0 13 139172.0 14 149447.0 15 155867.0 16 158653.0 17 163987.0 18 173288.0 19 183938.0 20 198537.0 21 212790.0 22 229492.0 23 261007.0 24 361330.0 25 398575.0 26 372706.0 27 547280.0 28 491878.0 29 693658.0 30 807992.0 31 1062107.0 32 1350655.0 33 1910135.0 34 2344381.0 35 4249081.0 36 4681181.0 37 4270786.0 38 1551445.0 39 333619.0 40 2104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.35876450363552 26.308722228850563 26.755134923675943 29.577378343837978 2 15.582227355054638 26.46169299364432 29.133586086372187 28.822493564928852 3 14.074590531606246 27.487387128748082 26.08935958649916 32.34866275314651 4 12.734921743898875 25.974770362633016 26.361304250187782 34.92900364328032 5 12.486842401030172 26.072220855718864 27.276903209652886 34.16403353359808 6 12.474468537052955 23.513596793521078 27.862150803815585 36.14978386561039 7 16.62889861787548 26.602212479054714 23.544819946806975 33.22406895626283 8 14.69217386416174 26.04970321303155 21.375978869272625 37.882144053534084 9 11.70404684340507 25.245184229782748 22.77873823247806 40.27203069433412 10-11 17.50968755624601 37.95552961894241 21.589269197146326 22.945513627665257 12-13 18.570486724243967 39.36753937250289 18.979031506068704 23.082942397184436 14-15 21.145901904378757 36.93189339806915 18.51562034634458 23.40658435120751 16-17 20.477468069557446 35.77057436642414 20.560783464062265 23.19117409995614 18-19 20.561347306098202 33.09733443438268 23.340662767438555 23.00065549208056 20-21 18.75432169047914 33.117658862446994 25.115971971335473 23.01204747573839 22-23 19.77528267534501 35.13562126391243 24.948372302655265 20.1407237580873 24-25 20.919154840325913 36.04364277138589 22.786836360670417 20.250366027617776 26-27 23.63761160830127 36.91231133768588 21.081516130987392 18.368560923025452 28-29 23.17939814296099 38.46798315237705 21.93075228992683 16.421866414735124 30-31 23.787069555542963 38.60648945038862 21.54989700387742 16.056543990190992 32-33 24.615093318742357 38.804555564524925 20.658066860028672 15.922284256704044 34-35 25.227905619278356 38.509796195288025 20.229898678037785 16.03239950739583 36-37 26.492020264478853 38.347019340517406 19.265562511818423 15.895397883185314 38-39 27.32095007595681 38.99705679363411 18.730499772141233 14.951493358267859 40-41 27.80271855799008 38.930501539440606 18.682692385259017 14.584087517310303 42-43 28.16113403363613 37.8636057297971 19.50075407351351 14.474506163053258 44-45 28.293189719848588 36.28719551514516 20.532857951990575 14.886756813015674 46-47 28.276787732780157 35.263540588255616 21.813293939919294 14.646377739044937 48-49 28.262764332926317 35.63574035627205 22.634537772487135 13.466957538314489 50-51 28.933137444121904 36.33976952412208 22.50504138421907 12.222051647536947 52-53 29.470580574602582 37.52157226746573 21.675642619323614 11.33220453860807 54-55 29.137523822430474 39.08722099534222 20.879884937089425 10.895370245137885 56-57 28.37240575984778 40.03509847073945 20.33478927427935 11.25770649513342 58-59 27.719032994328728 39.394160434771074 21.149104421209934 11.737702149690264 60-61 27.48522062294956 37.84274336960905 22.654676495778222 12.017359511663168 62-63 27.21795084457721 36.23215067420414 23.844960897893593 12.70493758332506 64-65 26.253850805484426 35.60752285026976 24.380337714233715 13.758288630012105 66-67 24.99313000022283 34.53762799581782 25.247900372284164 15.221341631675186 68-69 24.775082630673744 33.374843255435124 25.012763745301918 16.83731036858921 70-71 25.198373407793213 32.375996922531606 25.098993537466956 17.326636132208233 72-73 26.182853537664208 32.42137089776639 24.876964544332996 16.51881102023641 74-75 26.08842322125157 33.01032262041153 24.354742772463407 16.546511385873494 76-77 24.45436186893612 34.27308722360956 24.033066650181915 17.239484257272405 78-79 22.77351550683245 35.94248081171288 23.32654049308795 17.957463188366717 80-81 20.89693345829166 38.63268489102522 22.939518391678664 17.53086325900445 82-83 18.83476806806648 41.47681831710929 23.506270718045325 16.1821428967789 84-85 16.911194894014024 44.439054595758684 23.912703958016934 14.737046552210353 86-87 15.393632292970777 47.47176702281551 23.597378392785544 13.537222291428167 88-89 14.421263115785818 50.641606452880204 22.579020666477383 12.358109764856597 90-91 14.05908438676775 53.81919912228257 20.72111091052552 11.400605580424164 92-93 14.033638229730293 56.07952849320388 18.8783407975189 11.008492479546925 94-95 14.358455375506423 56.9940051479212 17.609914062844425 11.037625413727946 96-97 14.900155928762231 56.61731582001909 17.146399662790827 11.33612858842785 98-99 15.781667899280782 55.20282590419108 16.982672877648167 12.032833318879977 100 17.166070355993902 53.74133507063701 16.567054857557938 12.52553971581115 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1199608.0 1 1020853.0 2 530489.0 3 156154.0 4 90572.0 5 81987.0 6 66881.0 7 49673.5 8 38008.5 9 33745.5 10 35214.0 11 40902.5 12 50677.5 13 65675.0 14 87394.5 15 115038.0 16 145306.0 17 176619.5 18 207839.5 19 237453.5 20 263177.0 21 283607.0 22 300074.0 23 312492.5 24 321596.5 25 328203.5 26 329398.5 27 324484.0 28 315459.0 29 301702.0 30 284794.0 31 266739.5 32 248733.0 33 232091.0 34 216832.0 35 204512.5 36 196965.5 37 193959.0 38 198618.5 39 214661.5 40 245670.5 41 299815.5 42 387918.0 43 525417.5 44 722868.0 45 976029.5 46 1254933.0 47 1498274.0 48 1638783.5 49 1642110.0 50 1532756.5 51 1372917.5 52 1221346.5 53 1100516.0 54 996915.5 55 876439.0 56 722590.5 57 551300.5 58 384523.0 59 246015.0 60 146689.0 61 84659.5 62 50483.5 63 32460.5 64 23454.0 65 18552.5 66 15369.5 67 13288.0 68 11648.5 69 10207.0 70 9163.0 71 8378.5 72 7594.0 73 6880.5 74 6410.0 75 6056.5 76 5682.5 77 5350.5 78 4994.5 79 4726.0 80 4502.0 81 4171.0 82 3995.0 83 3939.0 84 3737.5 85 3563.0 86 3361.0 87 3151.0 88 3004.5 89 2821.0 90 2619.0 91 2458.0 92 2355.0 93 2190.5 94 1992.5 95 1774.5 96 1564.0 97 1402.0 98 1220.5 99 997.5 100 894.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.7566662346701743E-4 2 4.08693777045714E-4 3 6.094556324365911E-5 4 7.205916595279695E-4 5 6.022855661726312E-4 6 7.887072890355885E-4 7 8.532378854112276E-4 8 0.0012081561654772423 9 4.588842408934333E-4 10-11 3.6029582976398474E-4 12-13 8.24557620355388E-4 14-15 0.0024575402119722542 16-17 0.0012207037814391721 18-19 0.0034398392901347596 20-21 0.0036352235958276666 22-23 0.003633431079261677 24-25 0.004956308304962277 26-27 0.0069782669913989685 28-29 0.009213535149188466 30-31 0.009487790183784931 32-33 0.009093436539267136 34-35 0.011463143439505882 36-37 0.010651133435112424 38-39 0.009193817466962576 40-41 0.007600270239797488 42-43 0.004441856050523155 44-45 0.005273583737142503 46-47 0.007569797458175659 48-49 0.00615729440417556 50-51 0.003972216710233781 52-53 0.004167601015926689 54-55 0.004221376512906388 56-57 0.006924491494419269 58-59 0.00819180070657418 60-61 0.0078046171283203455 62-63 0.009322878659713853 64-65 0.010134888664107312 66-67 0.009143627003114857 68-69 0.01155993933406934 70-71 0.011787588937950068 72-73 0.013684071464767458 74-75 0.018073944534876905 76-77 0.019158417057300838 78-79 0.01904548851364347 80-81 0.023594895558126022 82-83 0.021759358594552292 84-85 0.02125386892294312 86-87 0.021391892698524345 88-89 0.022108899324920335 90-91 0.021347079784374594 92-93 0.02197625309903708 94-95 0.019360971429257707 96-97 0.021546049123199483 98-99 0.018586604272750037 100 0.01906520619586936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7893745E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.765797823583 #Duplication Level Percentage of deduplicated Percentage of total 1 95.92188072228929 83.22738509607997 2 3.6359670305152867 6.309551605258057 3 0.292747352850165 0.762013728923596 4 0.0593197416815555 0.2058769885475606 5 0.025373270106595344 0.11007660120960071 6 0.014242386825143106 0.07414512334773775 7 0.009020736885034426 0.05478840029506444 8 0.004825518776871179 0.03349519892703267 9 0.0047752034402114745 0.03728919026338802 >10 0.025379408830476742 0.41703404028522234 >50 0.0034966813923946144 0.2075999405664289 >100 0.0022431353246553675 0.3768133984794721 >500 2.861285331183606E-4 0.16937809426211467 >1k 2.9020555179961975E-4 0.5257450936650807 >5k 4.1209999311177886E-5 0.2685754300143875 >10k+ 1.1126699812197868E-4 7.220232069875208 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 869471 3.117082342295737 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 190774 0.6839311107203425 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 164057 0.5881497805332343 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 148149 0.5311190734696972 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 71868 0.25764916112913483 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 58472 0.2096240572931315 No Hit ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52508 0.18824291969400309 No Hit AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51622 0.18506658033906884 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44046 0.15790636933118876 No Hit AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40514 0.1452440323090356 No Hit CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 35963 0.12892854652539484 No Hit AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30370 0.108877456218231 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002448577629142304 0.0 0.0 1.0755099395939843E-5 3.5850331319799477E-6 2 0.006402869173716186 0.0 3.5850331319799477E-6 2.1510198791879685E-5 3.5850331319799477E-6 3 0.011145868007325657 0.0 3.5850331319799477E-6 4.660543071573932E-5 3.5850331319799477E-6 4 0.018814253876630764 0.0 3.5850331319799477E-6 5.736053011167916E-5 3.5850331319799477E-6 5 0.03212189686254033 0.0 3.5850331319799477E-6 6.0945563243659104E-5 3.5850331319799477E-6 6 0.054374197512739864 0.0 3.5850331319799477E-6 7.52856957715789E-5 3.5850331319799477E-6 7 0.08190725196634586 0.0 3.5850331319799477E-6 7.887072890355884E-5 3.5850331319799477E-6 8 0.18056377872530202 0.0 3.5850331319799477E-6 7.887072890355884E-5 3.5850331319799477E-6 9 0.5322411888400069 0.0 3.5850331319799477E-6 8.245576203553879E-5 3.5850331319799477E-6 10-11 1.3153647887725366 0.0 3.5850331319799477E-6 8.245576203553879E-5 3.5850331319799477E-6 12-13 2.4048904153959967 0.0 3.5850331319799477E-6 8.245576203553879E-5 3.5850331319799477E-6 14-15 3.7234799414707496 0.0 3.5850331319799477E-6 8.962582829949869E-5 3.5850331319799477E-6 16-17 5.138157318065394 0.0 3.5850331319799477E-6 9.500337799746861E-5 3.5850331319799477E-6 18-19 6.659338858944899 0.0 3.5850331319799477E-6 1.0755099395939843E-4 3.5850331319799477E-6 20-21 8.663782866015303 0.0 3.5850331319799477E-6 1.1472106022335832E-4 3.5850331319799477E-6 22-23 11.056290576973439 0.0 3.5850331319799477E-6 1.1472106022335832E-4 3.5850331319799477E-6 24-25 13.705253633027763 0.0 3.5850331319799477E-6 1.2009860992132825E-4 3.5850331319799477E-6 26-27 16.828036178003348 0.0 3.5850331319799477E-6 1.290611927512781E-4 3.5850331319799477E-6 28-29 20.96790337762104 3.5850331319799477E-6 3.5850331319799477E-6 1.290611927512781E-4 3.5850331319799477E-6 30-31 26.24372776047103 3.5850331319799477E-6 3.5850331319799477E-6 1.36231259015238E-4 3.5850331319799477E-6 32-33 31.9489190139223 5.377549697969921E-6 3.5850331319799477E-6 1.36231259015238E-4 3.5850331319799477E-6 34-35 36.66544417036866 7.170066263959895E-6 3.5850331319799477E-6 1.3981629214721795E-4 3.5850331319799477E-6 36-37 39.95792067361339 7.170066263959895E-6 3.5850331319799477E-6 1.3981629214721795E-4 3.5850331319799477E-6 38-39 42.1594088567168 7.170066263959895E-6 3.5850331319799477E-6 1.4877887497716783E-4 3.5850331319799477E-6 40-41 43.83587252267489 7.170066263959895E-6 3.5850331319799477E-6 1.6132649093909764E-4 3.5850331319799477E-6 42-43 45.072735840956454 7.170066263959895E-6 3.5850331319799477E-6 1.8821423942894723E-4 3.5850331319799477E-6 44-45 46.03099727196904 7.170066263959895E-6 5.377549697969921E-6 1.989693388248871E-4 3.5850331319799477E-6 46-47 46.812210049242225 7.170066263959895E-6 7.170066263959895E-6 2.0793192165483696E-4 3.5850331319799477E-6 48-49 47.50368048463912 7.170066263959895E-6 7.170066263959895E-6 2.3481967014468656E-4 3.5850331319799477E-6 50-51 48.203561407763644 7.170066263959895E-6 7.170066263959895E-6 2.563298689365663E-4 3.5850331319799477E-6 52-53 48.97184117801321 7.170066263959895E-6 7.170066263959895E-6 2.72462518030476E-4 3.5850331319799477E-6 54-55 49.795929159028304 7.170066263959895E-6 7.170066263959895E-6 3.0293529965230557E-4 3.5850331319799477E-6 56-57 50.68001446202366 7.170066263959895E-6 7.170066263959895E-6 3.2982304814215517E-4 3.5850331319799477E-6 58-59 51.58977039476055 7.170066263959895E-6 7.170066263959895E-6 3.4954073036804487E-4 3.5850331319799477E-6 60-61 52.48551601801766 7.170066263959895E-6 7.170066263959895E-6 3.8001351198987444E-4 3.5850331319799477E-6 62-63 53.31011486625407 7.170066263959895E-6 7.170066263959895E-6 4.08693777045714E-4 3.5850331319799477E-6 64-65 54.04852055541484 7.170066263959895E-6 7.170066263959895E-6 4.248264261396238E-4 3.5850331319799477E-6 66-67 54.683589457062865 7.170066263959895E-6 8.962582829949868E-6 4.6067675745942323E-4 3.5850331319799477E-6 68-69 55.21660142802625 1.0755099395939843E-5 1.0755099395939843E-5 4.76809406553333E-4 3.5850331319799477E-6 70-71 55.6843604184379 1.0755099395939843E-5 1.0755099395939843E-5 4.8756450594927283E-4 3.5850331319799477E-6 72-73 56.11092020809683 1.0755099395939843E-5 1.0755099395939843E-5 4.929420556472428E-4 3.5850331319799477E-6 74-75 56.513013939146575 1.0755099395939843E-5 1.0755099395939843E-5 5.126597378731325E-4 3.5850331319799477E-6 76-77 56.8941262637914 1.0755099395939843E-5 1.0755099395939843E-5 5.43132519494962E-4 3.5850331319799477E-6 78-79 57.25853950410746 1.0755099395939843E-5 1.0755099395939843E-5 6.112481490025811E-4 3.5850331319799477E-6 80-81 57.606386306320644 1.0755099395939843E-5 1.0755099395939843E-5 7.080440435660396E-4 7.170066263959895E-6 82-83 57.94831959638263 1.0755099395939843E-5 1.0755099395939843E-5 7.331392754898993E-4 7.170066263959895E-6 84-85 58.29024213134522 1.2547615961929817E-5 1.0755099395939843E-5 7.456868914518291E-4 7.170066263959895E-6 86-87 58.63291035319926 1.434013252791979E-5 1.0755099395939843E-5 7.564419908477689E-4 7.170066263959895E-6 88 58.88982996008603 1.434013252791979E-5 1.0755099395939843E-5 7.600270239797489E-4 7.170066263959895E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCTGA 15365 0.0 45.143616 9 GCTTGCT 3180 0.0 41.67319 7 TACCCGA 21685 0.0 41.0009 6 CTACCCG 21805 0.0 38.98642 5 ACCCGAC 23485 0.0 38.759113 7 CTGCTGA 14150 0.0 38.126595 9 CCTGCTG 10055 0.0 36.734856 8 CTTGCTG 10190 0.0 36.66324 8 GTTGCTG 4000 0.0 36.419968 8 CCCGACG 26845 0.0 35.203556 8 GCCTGCT 2985 0.0 32.27337 7 ATTGCTG 2685 0.0 30.80394 8 CGTACAC 9585 0.0 30.54434 7 TCGCGAC 1665 0.0 30.48184 6 GTACACG 13180 0.0 30.164272 8 ACTTGCT 2450 0.0 30.113995 7 CCCTGCA 13855 0.0 29.54218 5 CTCCACG 32430 0.0 29.372543 7 TTTGCTG 4230 0.0 29.107092 8 CTGCACG 22280 0.0 29.001623 7 >>END_MODULE