##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248495_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27893745
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27153037356583	34.0	31.0	34.0	30.0	34.0
2	32.54823810857954	34.0	31.0	34.0	31.0	34.0
3	32.66714021369307	34.0	31.0	34.0	31.0	34.0
4	36.1593732573378	37.0	35.0	37.0	35.0	37.0
5	36.06808910026244	37.0	35.0	37.0	35.0	37.0
6	36.05708459011151	37.0	35.0	37.0	35.0	37.0
7	36.049778292588535	37.0	35.0	37.0	35.0	37.0
8	36.04708109291169	37.0	35.0	37.0	35.0	37.0
9	37.758406839956415	39.0	38.0	39.0	35.0	39.0
10-11	37.77648954989729	39.0	38.0	39.0	35.0	39.0
12-13	37.7717256144702	39.0	38.0	39.0	35.0	39.0
14-15	39.17784021112977	41.0	39.0	41.0	36.0	41.0
16-17	39.069053205297465	41.0	39.0	41.0	36.0	41.0
18-19	38.89540621024534	40.0	38.0	41.0	35.0	41.0
20-21	38.672293895997115	40.0	38.0	41.0	34.5	41.0
22-23	38.502244858121415	40.0	37.5	41.0	34.0	41.0
24-25	38.34697624861774	40.0	37.0	41.0	34.0	41.0
26-27	38.17910248337037	40.0	37.0	41.0	34.0	41.0
28-29	38.028789895369016	40.0	36.5	41.0	33.0	41.0
30-31	37.968913550331806	40.0	36.0	41.0	33.0	41.0
32-33	37.90378511383108	40.0	36.0	41.0	33.0	41.0
34-35	37.8680126494309	40.0	36.0	41.0	33.0	41.0
36-37	37.77416153693238	40.0	36.0	41.0	33.0	41.0
38-39	37.68531202963245	40.0	35.5	41.0	33.0	41.0
40-41	37.53344074092597	40.0	35.0	41.0	33.0	41.0
42-43	37.35906641435203	39.5	35.0	41.0	32.0	41.0
44-45	37.1031639351403	39.0	35.0	41.0	32.0	41.0
46-47	36.7796035813764	39.0	35.0	41.0	31.5	41.0
48-49	36.41093490673268	37.5	35.0	40.0	31.0	41.0
50-51	36.006294887975784	37.0	35.0	40.0	31.0	41.0
52-53	35.58876626283061	36.5	35.0	39.0	30.5	41.0
54-55	35.251175272449075	35.5	35.0	39.0	30.5	41.0
56-57	34.91611830896139	35.0	35.0	37.5	30.5	40.0
58-59	34.578751544477086	35.0	34.0	37.0	30.0	40.0
60-61	34.281012087118455	35.0	34.0	36.0	30.0	39.5
62-63	34.03551186834181	35.0	34.0	35.5	30.0	39.0
64-65	33.830950630687994	35.0	34.0	35.0	29.0	39.0
66-67	33.6527701461385	35.0	34.0	35.0	29.0	37.5
68-69	33.5003204481865	35.0	33.0	35.0	29.0	37.0
70-71	33.42174890822297	35.0	33.0	35.0	29.0	36.5
72-73	33.346927976146624	35.0	33.0	35.0	29.0	36.0
74-75	33.26585980118482	35.0	33.5	35.0	29.0	36.0
76-77	33.15107919356114	35.0	33.0	35.0	29.0	36.0
78-79	33.00297330100351	35.0	33.0	35.0	29.0	36.0
80-81	32.875916787078964	35.0	33.0	35.0	29.0	35.0
82-83	32.79576824481617	35.0	33.0	35.0	28.5	35.0
84-85	32.71743012635987	35.0	33.0	35.0	27.0	35.0
86-87	32.64167730077119	35.0	33.0	35.0	27.0	35.0
88-89	32.531529900341454	35.0	33.0	35.0	27.0	35.0
90-91	32.44200491902396	35.0	33.0	35.0	27.0	35.0
92-93	32.29123900358306	35.0	33.0	35.0	26.0	35.0
94-95	32.035421113228075	35.0	33.0	35.0	25.0	35.0
96-97	31.80876540600769	35.0	33.0	35.0	24.0	35.0
98-99	31.550639543022996	35.0	33.0	35.0	23.5	35.0
100	30.294461320987914	34.0	30.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3060987069935095
1101	2	0.3071685201212091
1101	3	0.2855083751420153
1101	4	0.1786354032979034
1101	5	0.215290054820251
1101	6	0.2310064472661253
1101	7	0.25790971387544914
1101	8	0.23251338458690896
1101	9	0.34641126678356926
1101	10-11	0.3176301442524547
1101	12-13	0.32870357190462585
1101	14-15	0.442627690929875
1101	16-17	0.4534003177002006
1101	18-19	0.4727265850876634
1101	20-21	0.4287453974081572
1101	22-23	0.4456242270763866
1101	24-25	0.4580943922990812
1101	26-27	0.4745429596220845
1101	28-29	0.49528370648751263
1101	30-31	0.5020170248571105
1101	32-33	0.4847360587187879
1101	34-35	0.5075290271544262
1101	36-37	0.5311550558665559
1101	38-39	0.5407747716936697
1101	40-41	0.5364041824162769
1101	42-43	0.550330496717379
1101	44-45	0.5513704451040056
1101	46-47	0.5336027143549202
1101	48-49	0.524613748308532
1101	50-51	0.5153281838245647
1101	52-53	0.47498760044920374
1101	54-55	0.48668619660107737
1101	56-57	0.4803164903678123
1101	58-59	0.5035949301797444
1101	60-61	0.5207400904157282
1101	62-63	0.5083638502303316
1101	64-65	0.49714539948035963
1101	66-67	0.5260496782534076
1101	68-69	0.5656648270964837
1101	70-71	0.5387734533194504
1101	72-73	0.5187064478308372
1101	74-75	0.48396661520332174
1101	76-77	0.4890738638642773
1101	78-79	0.5209818841236142
1101	80-81	0.5556186730714927
1101	82-83	0.5354504907836315
1101	84-85	0.510424931991281
1101	86-87	0.4760113805855113
1101	88-89	0.4877531488280269
1101	90-91	0.5320475314291642
1101	92-93	0.5560806448867197
1101	94-95	0.5229549986852646
1101	96-97	0.5349412948348267
1101	98-99	0.4970581045936058
1101	100	0.5294433324798327
1102	1	0.25444268120869395
1102	2	0.25327421007136763
1102	3	0.2514340560722772
1102	4	0.16855002810625308
1102	5	0.2015127926964766
1102	6	0.19301277395823746
1102	7	0.2057081946776762
1102	8	0.21158429011914848
1102	9	0.2804746941055427
1102	10-11	0.2714866043031918
1102	12-13	0.2635465774478334
1102	14-15	0.37152024426473673
1102	16-17	0.3703913853228187
1102	18-19	0.4004664243657672
1102	20-21	0.39891841844370646
1102	22-23	0.3958247294679964
1102	24-25	0.3999900247097514
1102	26-27	0.40470270432675903
1102	28-29	0.42432867720089007
1102	30-31	0.4346342229282172
1102	32-33	0.4177905771510879
1102	34-35	0.44355272591081985
1102	36-37	0.4881011538526181
1102	38-39	0.47048784169740543
1102	40-41	0.45441716378905994
1102	42-43	0.4525161125419572
1102	44-45	0.42841000038837507
1102	46-47	0.4399530401560625
1102	48-49	0.4312013434066344
1102	50-51	0.43720815343722563
1102	52-53	0.39755254224061076
1102	54-55	0.3889225421420406
1102	56-57	0.3943454650300282
1102	58-59	0.39016828202041154
1102	60-61	0.39694322879809363
1102	62-63	0.3784826626602822
1102	64-65	0.37396412678826607
1102	66-67	0.39743848973365914
1102	68-69	0.3965391740463531
1102	70-71	0.3794542634986513
1102	72-73	0.36909907307401824
1102	74-75	0.3517571463331919
1102	76-77	0.35092523630912353
1102	78-79	0.4003412620536153
1102	80-81	0.4149868520152751
1102	82-83	0.4021083633191438
1102	84-85	0.3425316324031371
1102	86-87	0.34723547021514634
1102	88-89	0.3658722139890429
1102	90-91	0.42743509069310903
1102	92-93	0.41502619630791315
1102	94-95	0.4411462620251214
1102	96-97	0.417501616161406
1102	98-99	0.3965835358244192
1102	100	0.4066354858881027
1103	1	0.18657649094376438
1103	2	0.19872496481661273
1103	3	0.18788794201476122
1103	4	0.13782669990872165
1103	5	0.12965726791992438
1103	6	0.1555651681789456
1103	7	0.14700074083543768
1103	8	0.16092924850361356
1103	9	0.24123479963135708
1103	10-11	0.22724512440181144
1103	12-13	0.23435851252175155
1103	14-15	0.30351087220333994
1103	16-17	0.31851768621009313
1103	18-19	0.3295137046553762
1103	20-21	0.34065443337495793
1103	22-23	0.3350399466017038
1103	24-25	0.32046596593903587
1103	26-27	0.3469580959139691
1103	28-29	0.3583133020290177
1103	30-31	0.33128689071630646
1103	32-33	0.33488877701422837
1103	34-35	0.3781539174054487
1103	36-37	0.3668703188933691
1103	38-39	0.3636165875073516
1103	40-41	0.36472226616510994
1103	42-43	0.3620822412592517
1103	44-45	0.35074600281603097
1103	46-47	0.3557708717020134
1103	48-49	0.35689272928364346
1103	50-51	0.3248431827134226
1103	52-53	0.2994208686695785
1103	54-55	0.2888028300077181
1103	56-57	0.29356892931409817
1103	58-59	0.27253665823408113
1103	60-61	0.25514618709702575
1103	62-63	0.26938964498913975
1103	64-65	0.2817628198584501
1103	66-67	0.2894053546502775
1103	68-69	0.28770169549250113
1103	70-71	0.2751091794987559
1103	72-73	0.260107779302281
1103	74-75	0.25243307819277305
1103	76-77	0.26046541508178933
1103	78-79	0.2857054877522671
1103	80-81	0.32942452747309403
1103	82-83	0.29382259913413833
1103	84-85	0.26651487183670497
1103	86-87	0.27612926282738925
1103	88-89	0.28345594174158606
1103	90-91	0.3059015122028157
1103	92-93	0.330820431105991
1103	94-95	0.3642543932200155
1103	96-97	0.35223443891756645
1103	98-99	0.33507921264312657
1103	100	0.29518158444203024
1104	1	0.18222950368936353
1104	2	0.19307054430913695
1104	3	0.1966627883871439
1104	4	0.1287157636526146
1104	5	0.13364098415730297
1104	6	0.14355986187847236
1104	7	0.1481021801581477
1104	8	0.1309942605418044
1104	9	0.1985843759285757
1104	10-11	0.196860138666473
1104	12-13	0.196553027425594
1104	14-15	0.246975372114683
1104	16-17	0.26457918743462017
1104	18-19	0.2724813337649863
1104	20-21	0.265807364814016
1104	22-23	0.2252089122533718
1104	24-25	0.23318841687265035
1104	26-27	0.26570203381856317
1104	28-29	0.2544808495137687
1104	30-31	0.2498858599525846
1104	32-33	0.26638850786918766
1104	34-35	0.2906171577241565
1104	36-37	0.3060785437138591
1104	38-39	0.3087227622904507
1104	40-41	0.32341763714082816
1104	42-43	0.3292091122297123
1104	44-45	0.3084062745198892
1104	46-47	0.3137244284981122
1104	48-49	0.2941365790020356
1104	50-51	0.2717428580998913
1104	52-53	0.2604628821640418
1104	54-55	0.26466656481947126
1104	56-57	0.2600374595727146
1104	58-59	0.26172957257463025
1104	60-61	0.260622741187575
1104	62-63	0.2767623324715842
1104	64-65	0.2595563203249114
1104	66-67	0.27766413085443276
1104	68-69	0.2959084399553831
1104	70-71	0.2554735098454586
1104	72-73	0.2674820302800427
1104	74-75	0.2660007585267081
1104	76-77	0.24556200592611788
1104	78-79	0.27802216921018896
1104	80-81	0.29185720501803303
1104	82-83	0.26367942704789726
1104	84-85	0.23165683382276114
1104	86-87	0.2421459398597321
1104	88-89	0.2742124383333078
1104	90-91	0.3021970130225782
1104	92-93	0.3154297395368957
1104	94-95	0.2834211600866112
1104	96-97	0.2545406694635126
1104	98-99	0.24237077737712198
1104	100	0.24796235498304142
1105	1	0.14979795620043745
1105	2	0.14748243635079916
1105	3	0.1269464285480879
1105	4	0.08887069389687952
1105	5	0.09638821492179517
1105	6	0.0800874861942944
1105	7	0.09374814151237842
1105	8	0.08463413497191397
1105	9	0.15543266357774854
1105	10-11	0.15635344365789194
1105	12-13	0.1504947263419112
1105	14-15	0.19070877235325412
1105	16-17	0.2095554846232517
1105	18-19	0.21873109025955983
1105	20-21	0.1944684400615202
1105	22-23	0.18046306271113366
1105	24-25	0.1587340944153368
1105	26-27	0.17319615993742588
1105	28-29	0.2103128435133783
1105	30-31	0.19985856955933912
1105	32-33	0.19619110225939806
1105	34-35	0.22040784136838454
1105	36-37	0.23761213342746856
1105	38-39	0.25565742257419544
1105	40-41	0.24988264984191488
1105	42-43	0.2544983274683261
1105	44-45	0.2495559958824245
1105	46-47	0.24629831379949252
1105	48-49	0.213445829789201
1105	50-51	0.23106409336376998
1105	52-53	0.21928469298030961
1105	54-55	0.18234306194772643
1105	56-57	0.18930512752419304
1105	58-59	0.19763395139067796
1105	60-61	0.18144886033591234
1105	62-63	0.19827477149174655
1105	64-65	0.19103780990288044
1105	66-67	0.20595939919159179
1105	68-69	0.19547387105893677
1105	70-71	0.1933194042858304
1105	72-73	0.1910455555736874
1105	74-75	0.1662140016903848
1105	76-77	0.1766699229374069
1105	78-79	0.19781237683849895
1105	80-81	0.2081227796636682
1105	82-83	0.215407672991347
1105	84-85	0.17338015015577213
1105	86-87	0.16947046817065115
1105	88-89	0.1761658485345876
1105	90-91	0.19033961736513305
1105	92-93	0.20115270099228155
1105	94-95	0.22676226338801087
1105	96-97	0.2371326847622477
1105	98-99	0.2424192844334101
1105	100	0.22231294510048372
1106	1	0.13853178776739128
1106	2	0.1297100893744627
1106	3	0.13386683757997275
1106	4	0.07041695268886627
1106	5	0.10851847341948684
1106	6	0.08713821017066437
1106	7	0.09486996128815406
1106	8	0.09811041291655442
1106	9	0.1332183520853505
1106	10-11	0.12111031225045821
1106	12-13	0.13781809266146183
1106	14-15	0.1721669181843808
1106	16-17	0.16499498962373593
1106	18-19	0.18424326611731345
1106	20-21	0.17295997664290752
1106	22-23	0.16589103646433756
1106	24-25	0.16215322855191516
1106	26-27	0.15965571655720368
1106	28-29	0.2058987842170552
1106	30-31	0.2031395907960487
1106	32-33	0.21026097842242564
1106	34-35	0.235279275777728
1106	36-37	0.25599774996835833
1106	38-39	0.26109653703132807
1106	40-41	0.23162164718571887
1106	42-43	0.22623247673787006
1106	44-45	0.24450125520709065
1106	46-47	0.2234296817304866
1106	48-49	0.23231752707996378
1106	50-51	0.2136102510252016
1106	52-53	0.21823359831731892
1106	54-55	0.18558487360394338
1106	56-57	0.2038083822971899
1106	58-59	0.2042676950278377
1106	60-61	0.1803152332641389
1106	62-63	0.1753304661159305
1106	64-65	0.15959415555396816
1106	66-67	0.1869724971472735
1106	68-69	0.21044755133943482
1106	70-71	0.1702358076784094
1106	72-73	0.18788982976654012
1106	74-75	0.18599551557245064
1106	76-77	0.17705659743268143
1106	78-79	0.20462281213506373
1106	80-81	0.21012775031886122
1106	82-83	0.18466936347356722
1106	84-85	0.17947301093339263
1106	86-87	0.184081261316571
1106	88-89	0.2031867101599758
1106	90-91	0.21113294162719853
1106	92-93	0.19959213786162877
1106	94-95	0.21741844066867344
1106	96-97	0.20130427754626012
1106	98-99	0.19126900098304844
1106	100	0.17471774846294252
1107	1	0.10919021947155017
1107	2	0.11568717161001274
1107	3	0.09845469972491827
1107	4	0.0647037648989226
1107	5	0.0706334032795155
1107	6	0.04850596728094558
1107	7	0.06334127807821943
1107	8	0.0728179516280818
1107	9	0.09244975609273354
1107	10-11	0.11094660634851294
1107	12-13	0.11174858949824795
1107	14-15	0.14292740735457699
1107	16-17	0.15224271602863837
1107	18-19	0.1573773738690889
1107	20-21	0.1660204780662653
1107	22-23	0.15046283264454274
1107	24-25	0.12857959543005393
1107	26-27	0.15846093600330136
1107	28-29	0.17050197961971492
1107	30-31	0.1624455876420683
1107	32-33	0.1580221556581023
1107	34-35	0.210415640035869
1107	36-37	0.2344828253974569
1107	38-39	0.20555780620872355
1107	40-41	0.20018820620408917
1107	42-43	0.19334791662549833
1107	44-45	0.19334969559253068
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2216	64-65	-0.5231891726627325
2216	66-67	-0.47352127023725643
2216	68-69	-0.5785567735245252
2216	70-71	-0.4946880330927499
2216	72-73	-0.4587991560741429
2216	74-75	-0.45323386157065926
2216	76-77	-0.5172891220434579
2216	78-79	-0.4731532360890398
2216	80-81	-0.47104400746341923
2216	82-83	-0.5306697611033897
2216	84-85	-0.5249645895793478
2216	86-87	-0.5260726459435858
2216	88-89	-0.5448414445168837
2216	90-91	-0.6362579478276587
2216	92-93	-0.684273403646646
2216	94-95	-0.6672079559919339
2216	96-97	-0.6344486679599477
2216	98-99	-0.6935290453059402
2216	100	-0.7342711473573011
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	103.0
7	394.0
8	794.0
9	1376.0
10	2041.0
11	2981.0
12	4336.0
13	6026.0
14	9185.0
15	14620.0
16	23029.0
17	34405.0
18	48344.0
19	62166.0
20	77958.0
21	97720.0
22	119223.0
23	146254.0
24	178980.0
25	216065.0
26	261756.0
27	313082.0
28	376346.0
29	456354.0
30	538558.0
31	661828.0
32	843489.0
33	1142285.0
34	1620271.0
35	4065857.0
36	4921410.0
37	8870342.0
38	2299348.0
39	473671.0
40	3145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.776149518903477	23.307294758707048	29.83330414596677	27.0832515764227
2	17.486947875719963	24.34429435351521	34.849208576317906	23.31954919444692
3	15.255728479628678	21.91859142614231	35.844731498047324	26.980948596181687
4	15.940631705804714	20.142172381018483	37.65545779727526	26.261738115901544
5	15.218787679959028	20.545008806845978	39.23317146011882	25.003032053076186
6	14.632015170426202	15.284652526937492	35.983110191908615	34.10022211072769
7	19.81011872016468	16.632227045884303	32.91906482976739	30.638589404183627
8	17.100285386562472	15.368147948581305	32.530307421968615	35.001259242887606
9	17.199160600342477	15.946460398200385	36.186478366386446	30.667900635070694
10-11	20.434549035993555	24.386653710356928	35.063258805872074	20.11553844777745
12-13	19.31129362514786	31.966781441502384	27.892411004689404	20.82951392866035
14-15	21.565587553768776	27.36049963889754	28.20661944102522	22.86729336630847
16-17	28.247626831585976	26.674824894289834	30.303505211674516	14.77404306244968
18-19	28.07867677861112	19.70705798020309	30.352267148064914	21.861998093120878
20-21	22.281981085085746	19.97203182685108	36.59653184686471	21.14945524119847
22-23	22.786900790840384	23.077535841816864	37.07167323713614	17.06389013020661
24-25	23.881635497749503	25.43606880606109	32.97201921815095	17.710276478038462
26-27	27.957061699674963	25.296691068194683	28.8541606729394	17.892086559190957
28-29	25.0844571128371	28.88685286875985	31.819046300096765	14.209643718306289
30-31	24.612234659371154	27.263281990031146	31.800432599879098	16.324050750718605
32-33	25.204231626968994	28.352596551449448	31.060691613057546	15.382480208524013
34-35	25.675926628660672	28.186390382248362	30.813767753982614	15.323915235108352
36-37	26.60579280408565	27.190045653604418	29.993244005062785	16.21091753724715
38-39	27.316814811642715	27.634074867536746	28.747031339018264	16.302078981802275
40-41	27.613173724889084	27.66695823169233	28.770873358396205	15.948994685022386
42-43	27.759305942827346	27.096293450834313	29.276128712726646	15.868271893611697
44-45	27.822390081635778	25.22006348204815	29.837317280731273	17.12022915558479
46-47	27.2827264408674	24.530418785844134	30.96800157340278	17.218853199885682
48-49	25.72726007691985	25.09689102121544	30.90300114035199	18.272847761512725
50-51	25.73049167029144	25.56930839576646	30.180300844136454	18.51989908980565
52-53	25.345069396760998	26.92624333555593	29.740715630895924	17.98797163678714
54-55	25.038529723773532	27.649226262344424	29.82120763399667	17.491036379885376
56-57	24.619281965296295	27.591380600803962	29.480537859658853	18.30879957424089
58-59	24.100278542744206	27.38427476784759	29.821699446298783	18.693747243109424
60-61	23.556049103366565	26.617242535051517	29.841592710677013	19.985115650904905
62-63	23.03442581840481	26.770073362325498	29.85157962833603	20.343921190933667
64-65	22.332146352295464	26.913580304451173	29.943984910732997	20.810288432520366
66-67	21.343089642498704	26.3350313842763	31.38678940386097	20.935089569364028
68-69	20.424303415439923	25.613872459277783	33.09654366201566	20.865280463266636
70-71	19.225650165728783	25.17609949140417	34.86090559204459	20.73734475082246
72-73	18.677828950274215	24.949169081091995	35.739264435357164	20.633737533276623
74-75	18.960875999826268	25.18070012221595	35.59149116276733	20.266932715190443
76-77	20.06070111621178	25.56519440342864	35.27611823463783	19.097986245721753
78-79	20.591362106712584	25.547805672639917	35.91942565191248	17.941406568735015
80-81	19.84599307635303	24.533669471591075	37.412123914373296	18.208213537682603
82-83	19.232750666489757	23.268712128086204	38.49799453471768	19.00054267070636
84-85	19.05783266558912	22.30165523246945	38.8709766178091	19.769535484132327
86-87	19.54752768014501	22.0235706060593	38.794678643590444	19.634223070205252
88-89	19.63330033694107	22.512407794897108	38.33495375536772	19.519338112794102
90-91	19.75275553379675	22.57066724199396	37.7656030998841	19.910974124325186
92-93	19.15605861215448	22.536020451211165	37.638124591025516	20.669796345608834
94-95	18.396287903617687	23.221867643966114	38.08298400332299	20.298860449093205
96-97	17.236942625813423	24.615908970280316	38.90038564559693	19.246762758309334
98-99	16.929515303368696	26.283127889156766	38.43909414385238	18.34826266362216
100	16.736909225590935	28.10073771716021	37.64022788804183	17.522125169207023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	309830.0
1	654986.0
2	904522.5
3	548887.5
4	182766.5
5	57327.0
6	32239.0
7	24527.5
8	22403.0
9	23337.0
10	26890.5
11	34092.0
12	46150.5
13	63282.0
14	86331.5
15	115021.5
16	147378.0
17	181393.0
18	214926.0
19	245003.5
20	268224.5
21	284053.5
22	295964.5
23	305835.5
24	313216.5
25	318109.5
26	317876.5
27	313578.0
28	304366.5
29	289858.5
30	273789.0
31	255489.0
32	236846.5
33	218727.5
34	200062.0
35	181680.0
36	164414.0
37	148229.5
38	133445.0
39	119875.5
40	108659.0
41	102551.5
42	104741.0
43	123977.5
44	170895.5
45	249710.5
46	355591.5
47	477103.5
48	617393.5
49	804046.5
50	1073563.5
51	1444668.5
52	1878773.0
53	2258873.0
54	2427855.0
55	2281053.5
56	1848404.0
57	1284045.0
58	771789.5
59	413013.5
60	203825.0
61	96252.0
62	43890.0
63	19598.5
64	8906.5
65	4403.5
66	2628.0
67	1831.5
68	1384.5
69	1074.0
70	797.0
71	593.5
72	462.5
73	338.5
74	272.0
75	220.5
76	156.5
77	110.5
78	75.5
79	49.0
80	32.5
81	28.5
82	18.5
83	8.5
84	7.5
85	10.0
86	10.5
87	6.0
88	3.5
89	2.5
90	2.5
91	5.0
92	3.5
93	1.0
94	0.5
95	1.5
96	2.0
97	0.5
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0587479379337554
2	0.005528121089513079
3	0.0
4	3.5850331319799477E-6
5	3.728434457259145E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.245576203553879E-5
18-19	0.0
20-21	9.088058989569167E-4
22-23	0.0
24-25	3.764284788578945E-5
26-27	0.0
28-29	7.170066263959895E-6
30-31	2.545373523705763E-4
32-33	6.13040665568571E-4
34-35	2.652924517665161E-4
36-37	0.0
38-39	2.384047032766665E-4
40-41	8.783331173350871E-5
42-43	2.778400677284459E-4
44-45	1.1651357678934829E-4
46-47	2.1868702105077678E-4
48-49	5.897379502107014E-4
50-51	1.1472106022335832E-4
52-53	2.868026505583958E-5
54-55	7.349317920558892E-5
56-57	1.3443874244924804E-4
58-59	3.5850331319799477E-6
60-61	5.736053011167916E-5
62-63	0.0
64-65	3.943536445177942E-5
66-67	0.0
68-69	1.0755099395939843E-5
70-71	0.0010038092769543855
72-73	0.0010360745751422048
74-75	3.1010536591626547E-4
76-77	8.604079516751874E-5
78-79	9.930541775584453E-4
80-81	2.2944212044671665E-4
82-83	7.815372227716285E-4
84-85	2.868026505583958E-4
86-87	3.7642847885789447E-4
88-89	0.0010450371579721546
90-91	2.04346888522857E-4
92-93	1.5236390810914778E-4
94-95	0.0029648224001474167
96-97	4.642617905914032E-4
98-99	0.0015845846443351369
100	0.001430428219659999
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.7893745E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.87952079386845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.32324212816754	81.86314354484921
2	4.116322513058329	7.070156097089233
3	0.3838613108458346	0.9889747628023936
4	0.07735188764808228	0.2657177217487383
5	0.022174416046088077	0.09521641119609556
6	0.01779569335417954	0.0916971370470941
7	0.00988003193132359	0.059394468538313205
8	0.007443788212873642	0.05114151716901079
9	0.005062586131455232	0.03912952238523537
>10	0.029229355175143013	0.4777960424777848
>50	0.003519231850414463	0.20882787004594255
>100	0.0029519304846332165	0.5014352718151915
>500	3.2628134994491304E-4	0.20376338876360697
>1k	4.1371236487575255E-4	0.8481156865146842
>5k	1.625509979796724E-4	1.0300866167025642
>10k+	2.6258238129573357E-4	6.20540394085491
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71408	0.25600004588842407	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55773	0.19994805286991763	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53545	0.1919605990518663	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53247	0.19089225917853628	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49620	0.17788934400884499	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48791	0.17491735154243362	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44826	0.16070269517413313	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44775	0.16051985848440214	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42692	0.1530522344704879	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41820	0.1499260855794014	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41715	0.1495496571005435	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41119	0.14741297735388345	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40741	0.14605783482999504	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39516	0.14166616924331962	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38765	0.13897380936120268	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38089	0.13655032696398423	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36203	0.12978895447707003	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35911	0.1287421248025319	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35098	0.12582749286623218	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33721	0.12089090224349582	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33599	0.12045352820139427	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31902	0.11436972697642428	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31450	0.11274929200076934	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31182	0.11178850312139872	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30919	0.11084563940768799	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30490	0.1093076601940686	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30444	0.10914274866999753	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29238	0.1048191987128297	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28807	0.10327404943294635	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002326686502654986	0.0	0.0	0.0	0.0
2	0.005750393143695836	0.0	0.0	3.5850331319799477E-6	0.0
3	0.009944881908112374	0.0	0.0	7.170066263959895E-6	0.0
4	0.01692494141607733	0.0	0.0	1.0755099395939843E-5	0.0
5	0.03012144837489552	0.0	0.0	1.0755099395939843E-5	0.0
6	0.0561021834823542	0.0	0.0	1.0755099395939843E-5	0.0
7	0.09080530419992008	0.0	0.0	1.0755099395939843E-5	0.0
8	0.24819542876010375	0.0	0.0	1.0755099395939843E-5	0.0
9	0.8295121361437842	0.0	0.0	1.0755099395939843E-5	0.0
10-11	1.9803454143572332	0.0	0.0	1.2547615961929817E-5	0.0
12-13	3.262269014074661	3.5850331319799477E-6	0.0	1.7925165659899737E-5	0.0
14-15	4.684928466937659	3.5850331319799477E-6	0.0	2.5095231923859633E-5	0.0
16-17	6.06269434240544	3.5850331319799477E-6	0.0	2.868026505583958E-5	0.0
18-19	7.512318621970625	3.5850331319799477E-6	0.0	3.40578147538095E-5	0.0
20-21	9.427568797233931	3.5850331319799477E-6	0.0	3.5850331319799474E-5	0.0
22-23	11.804388403206525	3.5850331319799477E-6	1.7925165659899738E-6	3.5850331319799474E-5	0.0
24-25	14.671966779648987	3.5850331319799477E-6	3.5850331319799477E-6	3.5850331319799474E-5	0.0
26-27	18.179939624457024	3.5850331319799477E-6	3.5850331319799477E-6	3.5850331319799474E-5	0.0
28-29	22.840713930667967	3.5850331319799477E-6	3.5850331319799477E-6	3.943536445177942E-5	0.0
30-31	28.562310295731173	3.5850331319799477E-6	3.5850331319799477E-6	4.1227881017769396E-5	0.0
32-33	34.72813528624428	3.5850331319799477E-6	3.5850331319799477E-6	4.660543071573932E-5	0.0
34-35	39.908639015664626	3.5850331319799477E-6	3.5850331319799477E-6	4.660543071573932E-5	0.0
36-37	43.426434851254285	3.5850331319799477E-6	3.5850331319799477E-6	5.556801354568919E-5	0.0
38-39	45.70572363087137	3.5850331319799477E-6	3.5850331319799477E-6	6.453059637563905E-5	0.0
40-41	47.49986600938669	3.5850331319799477E-6	3.5850331319799477E-6	6.632311294162903E-5	0.0
42-43	48.84581113077502	3.5850331319799477E-6	3.5850331319799477E-6	8.424827860152877E-5	0.0
44-45	49.89083574113121	3.5850331319799477E-6	3.5850331319799477E-6	9.500337799746861E-5	0.0
46-47	50.73280586740862	3.5850331319799477E-6	3.5850331319799477E-6	9.679589456345858E-5	0.0
48-49	51.445544511860994	3.5850331319799477E-6	3.5850331319799477E-6	1.0396596082741848E-4	0.0
50-51	52.12447091632909	3.5850331319799477E-6	3.5850331319799477E-6	1.1830609335533826E-4	0.0
52-53	52.84074440344959	3.5850331319799477E-6	3.5850331319799477E-6	1.4519384184518786E-4	0.0
54-55	53.52571517377821	3.5850331319799477E-6	3.5850331319799477E-6	1.6849655720305753E-4	0.0
56-57	54.211754283980156	3.5850331319799477E-6	3.5850331319799477E-6	1.7208159033503748E-4	0.0
58-59	54.85885634933567	3.5850331319799477E-6	3.5850331319799477E-6	1.7208159033503748E-4	0.0
60-61	55.47503033386158	3.5850331319799477E-6	3.5850331319799477E-6	1.7566662346701743E-4	0.0
62-63	56.07519893796979	3.5850331319799477E-6	3.5850331319799477E-6	1.846292062969673E-4	0.0
64-65	56.64750466457623	3.5850331319799477E-6	3.5850331319799477E-6	1.9717682225889712E-4	0.0
66-67	57.15409852639006	3.5850331319799477E-6	3.5850331319799477E-6	2.258570873147367E-4	0.0
68-69	57.59048847689688	3.5850331319799477E-6	3.5850331319799477E-6	2.509523192385963E-4	0.0
70-71	57.99102630356734	3.5850331319799477E-6	3.5850331319799477E-6	2.652924517665161E-4	0.0
72-73	58.37203824728447	3.5850331319799477E-6	3.5850331319799477E-6	2.688774848984961E-4	0.0
74-75	58.733262600629644	5.377549697969921E-6	3.5850331319799477E-6	2.814251008604259E-4	0.0
76-77	59.08072221926457	7.170066263959895E-6	3.5850331319799477E-6	3.1189788248225546E-4	0.0
78-79	59.402688667297994	7.170066263959895E-6	3.5850331319799477E-6	3.459556972360649E-4	0.0
80-81	59.70989732644362	7.170066263959895E-6	3.5850331319799477E-6	3.979386776497742E-4	0.0
82-83	60.01382926530661	7.170066263959895E-6	3.5850331319799477E-6	4.08693777045714E-4	0.0
84-85	60.31651182012311	7.170066263959895E-6	3.5850331319799477E-6	4.1407132674368394E-4	0.0
86-87	60.61924277288689	7.170066263959895E-6	5.377549697969921E-6	4.1944887644165384E-4	0.0
88	60.84547987371362	1.0755099395939843E-5	7.170066263959895E-6	4.230339095736338E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCCTA	23510	0.0	60.232857	6
CCCCTAC	150565	0.0	55.84982	7
GCCCTAC	58790	0.0	55.72866	7
TGCTCTA	20670	0.0	55.18439	6
CCCCCTA	80740	0.0	53.687157	6
CTCCCTA	51020	0.0	53.60371	6
GCCCCTA	16720	0.0	53.351536	6
TCCCCTA	46295	0.0	51.63322	6
CCGCCTA	9835	0.0	51.228	6
GCCTTTT	3330	0.0	50.417484	1
TTGCCCT	10440	0.0	49.02472	5
TTGCTCT	7535	0.0	48.963688	5
CGCCCTA	24255	0.0	48.829945	6
CCCGGAA	14600	0.0	48.801517	6
CACCCTA	28570	0.0	48.56161	6
ACCCTAC	50010	0.0	48.023193	7
ATGCCCT	7605	0.0	47.956802	5
CTGCGTC	4685	0.0	47.04898	6
GTCCCTA	5355	0.0	47.042706	6
CCACACT	29220	0.0	46.13026	8
>>END_MODULE