FastQCFastQC Report
Thu 26 May 2016
SRR1248494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21326695
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT743080.34842717073601887No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT596150.27953229508838573No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT563860.2643916462442962No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT555960.260687368577269No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT525240.24628288630751272No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520250.24394309573049175No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT474220.22235981712121827No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT468170.21952299688254556No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448880.2104779948322982No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445550.20891657146126016No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438060.20540454111619266No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429170.20123605650101906No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422310.19801943057749924No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412690.193508651950056No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388610.18221763850423145No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386230.1811016662450511No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370070.17352430838439806No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358670.16817889504210567No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351500.1648169113873481No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346080.16227549557022314No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341330.16004824001093465No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334950.1570566841228798No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325160.1524661931912094No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315170.14778192307809532No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310370.1455312227234459No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302270.141733165874975No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299310.14034523398960785No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296820.13917768318063348No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296810.1391729942215613No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285610.13392136006071265No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271560.1273333725642909No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219720.10302580873407718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTA158650.060.3441736
GCCCTAC403950.055.2068567
CCCCTAC1049000.054.8883867
GCCCCTA109900.054.6106836
TGCTCTA148100.053.0280726
CCCCCTA571850.051.9831776
GCCTTTT25150.051.42431
CTCCCTA380050.051.2958686
ATGCCCT51400.050.016085
CGCCCTA162750.049.8430066
TCCCCTA336950.049.0838056
TTGCTCT54050.048.694265
CCGCCTA71650.046.637776
TTGCCCT71150.046.5032355
CCGCCCT92400.046.3882835
GTCCCTA42250.046.386736
CCCGGAA89450.046.1316036
CTGCCCT79750.045.9673355
CACCCTA215900.045.888346
ACCCTAC376900.045.5021747
Produced by FastQC (version 0.11.5)