##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248494_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21326695
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28138471525944	34.0	31.0	34.0	30.0	34.0
2	32.56807283078789	34.0	31.0	34.0	31.0	34.0
3	32.6910376408534	34.0	31.0	34.0	31.0	34.0
4	36.179242306414565	37.0	35.0	37.0	35.0	37.0
5	36.090867994314166	37.0	35.0	37.0	35.0	37.0
6	36.08021735200883	37.0	35.0	37.0	35.0	37.0
7	36.07071161284015	37.0	36.0	37.0	35.0	37.0
8	36.065601022568195	37.0	35.0	37.0	35.0	37.0
9	37.790702497503716	39.0	38.0	39.0	35.0	39.0
10-11	37.80816270406643	39.0	38.0	39.0	35.0	39.0
12-13	37.80038339273854	39.0	38.0	39.0	35.0	39.0
14-15	39.215089961196526	41.0	39.0	41.0	36.0	41.0
16-17	39.109061483741385	41.0	39.0	41.0	36.0	41.0
18-19	38.913121606512405	40.0	38.0	41.0	35.0	41.0
20-21	38.67223765801499	40.0	38.0	41.0	35.0	41.0
22-23	38.47372466760555	40.0	37.5	41.0	34.0	41.0
24-25	38.290588977804575	40.0	37.0	41.0	34.0	41.0
26-27	38.11933023377509	40.0	36.0	41.0	34.0	41.0
28-29	37.96947783048428	40.0	36.0	41.0	33.0	41.0
30-31	37.9052099024251	40.0	36.0	41.0	33.0	41.0
32-33	37.83456836607829	40.0	35.5	41.0	33.0	41.0
34-35	37.799994302914726	40.0	35.0	41.0	33.0	41.0
36-37	37.715993125048215	40.0	35.0	41.0	33.0	41.0
38-39	37.643360586344954	40.0	35.0	41.0	33.0	41.0
40-41	37.49937428185661	40.0	35.0	41.0	33.0	41.0
42-43	37.32834487481534	39.5	35.0	41.0	32.5	41.0
44-45	37.08046056831591	39.0	35.0	41.0	32.0	41.0
46-47	36.77028972374763	39.0	35.0	41.0	31.5	41.0
48-49	36.41265979093338	38.0	35.0	40.0	31.0	41.0
50-51	36.01737796222059	37.0	35.0	40.0	31.0	41.0
52-53	35.6111263840928	36.5	35.0	39.0	30.5	41.0
54-55	35.278206351241955	35.5	35.0	39.0	30.5	41.0
56-57	34.953487143694794	35.0	35.0	37.5	30.0	40.5
58-59	34.62936113635985	35.0	34.0	37.0	30.0	40.0
60-61	34.33676157979471	35.0	34.0	36.5	30.0	40.0
62-63	34.08966065768747	35.0	34.0	36.0	29.0	39.0
64-65	33.88293451001198	35.0	34.0	35.5	29.0	39.0
66-67	33.700126367447	35.0	34.0	35.0	29.0	39.0
68-69	33.54277343488994	35.0	33.5	35.0	29.0	37.5
70-71	33.44796237766799	35.0	33.0	35.0	29.0	37.0
72-73	33.35635437183305	35.0	33.0	35.0	29.0	36.5
74-75	33.262461764469364	35.0	33.0	35.0	29.0	36.0
76-77	33.15294599092827	35.0	33.0	35.0	29.0	36.0
78-79	33.01677533720063	35.0	33.0	35.0	29.0	36.0
80-81	32.88360280390374	35.0	33.0	35.0	29.0	36.0
82-83	32.779199425883846	35.0	33.0	35.0	27.5	35.5
84-85	32.6725605397367	35.0	33.0	35.0	27.0	35.0
86-87	32.58306015067032	35.0	33.0	35.0	27.0	35.0
88-89	32.471664385878825	35.0	33.0	35.0	27.0	35.0
90-91	32.382520381146726	35.0	33.0	35.0	27.0	35.0
92-93	32.23386509255185	35.0	33.0	35.0	25.5	35.0
94-95	31.981040404994772	35.0	33.0	35.0	24.5	35.0
96-97	31.757318304594314	35.0	33.0	35.0	24.0	35.0
98-99	31.50168689991581	35.0	32.5	35.0	21.5	35.0
100	30.253986752283932	34.0	30.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2898505890235299
1101	2	0.27824696931657655
1101	3	0.27182863674227065
1101	4	0.1640469569820837
1101	5	0.20350074492771597
1101	6	0.21752124715239773
1101	7	0.2104153111849314
1101	8	0.21210889614234674
1101	9	0.31908388008429966
1101	10-11	0.28711941669550356
1101	12-13	0.28968950282030903
1101	14-15	0.3982471338180531
1101	16-17	0.4128782713981778
1101	18-19	0.42095932754453713
1101	20-21	0.396063488794411
1101	22-23	0.41582565644446134
1101	24-25	0.4187238851298929
1101	26-27	0.46595249253648063
1101	28-29	0.4575339497256792
1101	30-31	0.4627681475653844
1101	32-33	0.45837734142399
1101	34-35	0.5100985402151963
1101	36-37	0.5264727926944985
1101	38-39	0.5100230099658702
1101	40-41	0.5170045821323015
1101	42-43	0.5279926098646328
1101	44-45	0.523315647788344
1101	46-47	0.5064294813988042
1101	48-49	0.5049740732193371
1101	50-51	0.481733994076059
1101	52-53	0.44291839860134985
1101	54-55	0.47950619855144794
1101	56-57	0.47081024541456173
1101	58-59	0.4698140205174113
1101	60-61	0.4615907995599784
1101	62-63	0.46334135575119006
1101	64-65	0.4623039661193076
1101	66-67	0.44724499575197285
1101	68-69	0.48102202458010623
1101	70-71	0.4585291686213182
1101	72-73	0.4404040983363231
1101	74-75	0.42744659795924633
1101	76-77	0.44710133219557235
1101	78-79	0.46208547556539514
1101	80-81	0.49670802139367964
1101	82-83	0.4721598524947481
1101	84-85	0.44542622383604424
1101	86-87	0.41400896869364345
1101	88-89	0.46969526014223817
1101	90-91	0.5115230927375052
1101	92-93	0.5220894316642344
1101	94-95	0.5291905593620854
1101	96-97	0.5675418422697369
1101	98-99	0.5221008504015572
1101	100	0.5114610573705036
1102	1	0.273514028606904
1102	2	0.26302722509609566
1102	3	0.252614988061886
1102	4	0.183475681631883
1102	5	0.2083566120516025
1102	6	0.19101851831566563
1102	7	0.20489702872826854
1102	8	0.20923862289227202
1102	9	0.2706719747950004
1102	10-11	0.2647171809979483
1102	12-13	0.2685735659807591
1102	14-15	0.3594590637559847
1102	16-17	0.36774014079706063
1102	18-19	0.36764326379785217
1102	20-21	0.3717050910221076
1102	22-23	0.3674919172969382
1102	24-25	0.3825643851640379
1102	26-27	0.38304417712059546
1102	28-29	0.4035510771940807
1102	30-31	0.4148733339373578
1102	32-33	0.4159102764931717
1102	34-35	0.4468542639439974
1102	36-37	0.4626932480613206
1102	38-39	0.4810390199185903
1102	40-41	0.4615442272919381
1102	42-43	0.47358562097817014
1102	44-45	0.46046195573969584
1102	46-47	0.4447634037456254
1102	48-49	0.4154007810114706
1102	50-51	0.42873426221321154
1102	52-53	0.4049858758971254
1102	54-55	0.41101940805316417
1102	56-57	0.39117420578355677
1102	58-59	0.41471726297834266
1102	60-61	0.42242598848008583
1102	62-63	0.4032518878785183
1102	64-65	0.3967302309022429
1102	66-67	0.42679950836690494
1102	68-69	0.463038582465245
1102	70-71	0.43463366216687405
1102	72-73	0.39536130464703945
1102	74-75	0.3721963094203602
1102	76-77	0.37058216538595445
1102	78-79	0.39992055956226835
1102	80-81	0.42106953355394694
1102	82-83	0.3947370401857526
1102	84-85	0.32985948017015687
1102	86-87	0.332392518063358
1102	88-89	0.34365370388090355
1102	90-91	0.4046974135261223
1102	92-93	0.4037606655447732
1102	94-95	0.4386213991309873
1102	96-97	0.4224795461830446
1102	98-99	0.4276898577778212
1102	100	0.4556243045946182
1103	1	0.19685930441350052
1103	2	0.19403331062675733
1103	3	0.20453517782863173
1103	4	0.14049647114102015
1103	5	0.1193563599726204
1103	6	0.15201027523298904
1103	7	0.15581799680927588
1103	8	0.16941855712944687
1103	9	0.23854886784162233
1103	10-11	0.21952330988068525
1103	12-13	0.21115683790415574
1103	14-15	0.29938497218246596
1103	16-17	0.31119606893376073
1103	18-19	0.31905888924886483
1103	20-21	0.30694264118709924
1103	22-23	0.30467715261088557
1103	24-25	0.3068223766392819
1103	26-27	0.3083239005433853
1103	28-29	0.31992139419216414
1103	30-31	0.33005935697121913
1103	32-33	0.34223765499595515
1103	34-35	0.39028512216589917
1103	36-37	0.39309102800921636
1103	38-39	0.38827787616487086
1103	40-41	0.39270547184685967
1103	42-43	0.3560252742158241
1103	44-45	0.3813210084480758
1103	46-47	0.3843343470409195
1103	48-49	0.3801586970199864
1103	50-51	0.36728032020013046
1103	52-53	0.33401911395322514
1103	54-55	0.33943056498554824
1103	56-57	0.32637107873323146
1103	58-59	0.34863235678234616
1103	60-61	0.31410235015945887
1103	62-63	0.315000324170434
1103	64-65	0.3309750800405169
1103	66-67	0.34315042732220036
1103	68-69	0.36133342063890694
1103	70-71	0.3759695418949818
1103	72-73	0.36276200413107773
1103	74-75	0.3288582196306393
1103	76-77	0.321695578356227
1103	78-79	0.37186103970581996
1103	80-81	0.3775954928975196
1103	82-83	0.36031124940583936
1103	84-85	0.33705214050320365
1103	86-87	0.32166013615042033
1103	88-89	0.3266625355824928
1103	90-91	0.3485087897354475
1103	92-93	0.36163647092876516
1103	94-95	0.3752171528185926
1103	96-97	0.39276660822715215
1103	98-99	0.35906993177559343
1103	100	0.29652281342965736
1104	1	0.14304882831503818
1104	2	0.1617645724900285
1104	3	0.16083245586629857
1104	4	0.10821395580771309
1104	5	0.14072724166917538
1104	6	0.12220528872123992
1104	7	0.1431743358798485
1104	8	0.14193800903853315
1104	9	0.18693409156770713
1104	10-11	0.18547443783445772
1104	12-13	0.19152711260252175
1104	14-15	0.24794934227620047
1104	16-17	0.23995249257692564
1104	18-19	0.25569550007820396
1104	20-21	0.24403671143103622
1104	22-23	0.21544777375681434
1104	24-25	0.21000541042638332
1104	26-27	0.2535413983331978
1104	28-29	0.25429082907734823
1104	30-31	0.23307914911463
1104	32-33	0.1985706438601298
1104	34-35	0.2503485465517201
1104	36-37	0.28126209126174473
1104	38-39	0.27679908475749215
1104	40-41	0.263980273978909
1104	42-43	0.27438508877183665
1104	44-45	0.2835483662129903
1104	46-47	0.25563816453167476
1104	48-49	0.2659743276189914
1104	50-51	0.24896655573042636
1104	52-53	0.24915331931971707
1104	54-55	0.22739797011495
1104	56-57	0.23221412349172965
1104	58-59	0.2382084294825475
1104	60-61	0.23836663591059448
1104	62-63	0.2362534683353985
1104	64-65	0.23735217282074927
1104	66-67	0.24132328576069995
1104	68-69	0.24692963526037914
1104	70-71	0.24364049460052684
1104	72-73	0.24384170110924686
1104	74-75	0.22673345970319758
1104	76-77	0.2206219412277335
1104	78-79	0.26811911577418357
1104	80-81	0.27301471786234544
1104	82-83	0.26352869288602676
1104	84-85	0.2412372408745611
1104	86-87	0.22089736359450995
1104	88-89	0.21649395616406508
1104	90-91	0.2535199629645746
1104	92-93	0.2624455611470822
1104	94-95	0.28214896892926333
1104	96-97	0.3151805534491565
1104	98-99	0.31530130700669545
1104	100	0.34151539375819695
1105	1	0.1348169128818455
1105	2	0.14766987269129572
1105	3	0.14540549600828712
1105	4	0.10960665101050893
1105	5	0.11109317994492329
1105	6	0.09033121052735282
1105	7	0.12318253773780441
1105	8	0.11403839406218452
1105	9	0.16644153549762564
1105	10-11	0.14546067547756536
1105	12-13	0.16160733579415876
1105	14-15	0.19375275309320728
1105	16-17	0.20960678134431276
1105	18-19	0.21295334098557817
1105	20-21	0.19676613053297842
1105	22-23	0.18937767595836874
1105	24-25	0.18389082648695876
1105	26-27	0.1715492310197746
1105	28-29	0.2202111043687296
1105	30-31	0.20205592210509593
1105	32-33	0.1946218719039905
1105	34-35	0.21453279094254896
1105	36-37	0.2628018832438599
1105	38-39	0.2799973982643351
1105	40-41	0.26764037973689625
1105	42-43	0.24300408060759793
1105	44-45	0.23795773161415212
1105	46-47	0.24136029862275876
1105	48-49	0.23363306881587675
1105	50-51	0.21992115169445015
1105	52-53	0.22266497611495595
1105	54-55	0.229471807292839
1105	56-57	0.2190804781633915
1105	58-59	0.21091555952319396
1105	60-61	0.2385000842576659
1105	62-63	0.2264875815111438
1105	64-65	0.20790475338615977
1105	66-67	0.24360849032446197
1105	68-69	0.23473434455861053
1105	70-71	0.21606287348316755
1105	72-73	0.22014809984862183
1105	74-75	0.2142946640283867
1105	76-77	0.19635344145620337
1105	78-79	0.23236014671950045
1105	80-81	0.20678090618387301
1105	82-83	0.20852848265833757
1105	84-85	0.19079807704044072
1105	86-87	0.213163729661332
1105	88-89	0.19709739078343347
1105	90-91	0.18542632249487667
1105	92-93	0.24580499930083022
1105	94-95	0.22251507079256072
1105	96-97	0.2495062499307643
1105	98-99	0.24312778383854194
1105	100	0.2488420408523062
1106	1	0.07295073396447549
1106	2	0.0902044612400772
1106	3	0.10814392778910786
1106	4	0.07765462495632391
1106	5	0.09230909942988319
1106	6	0.08569893029321918
1106	7	0.08585768101555402
1106	8	0.08522785097260765
1106	9	0.11835130312589115
1106	10-11	0.11795601668020339
1106	12-13	0.1294305039691821
1106	14-15	0.14418445184217887
1106	16-17	0.13998046294934596
1106	18-19	0.15809773499137236
1106	20-21	0.1677923114519686
1106	22-23	0.15166526445224804
1106	24-25	0.1584753121480631
1106	26-27	0.13302379092588978
1106	28-29	0.1797751420561582
1106	30-31	0.1784131544226284
1106	32-33	0.17460230608143235
1106	34-35	0.20969807649938588
1106	36-37	0.23767844905143676
1106	38-39	0.23579635874449423
1106	40-41	0.1860175226720031
1106	42-43	0.18594971972248686
1106	44-45	0.19778732806486943
1106	46-47	0.18963631311346774
1106	48-49	0.1818850403185479
1106	50-51	0.18346314880937342
1106	52-53	0.15160368928219725
1106	54-55	0.1566183714189009
1106	56-57	0.16139480466583223
1106	58-59	0.12737268291866854
1106	60-61	0.15432468458737958
1106	62-63	0.16430678267830956
1106	64-65	0.12708396801773603
1106	66-67	0.1636221927410304
1106	68-69	0.16400523719874371
1106	70-71	0.1325493639449391
1106	72-73	0.1722539566536483
1106	74-75	0.1823500987101312
1106	76-77	0.16624661146801145
1106	78-79	0.1694869590156145
1106	80-81	0.16995676725723996
1106	82-83	0.14689331082266222
1106	84-85	0.1253895794555433
1106	86-87	0.14480447162623733
1106	88-89	0.15802072175281978
1106	90-91	0.15476120206005817
1106	92-93	0.17655377770858394
1106	94-95	0.18328462309146687
1106	96-97	0.15987157385076856
1106	98-99	0.1438748990387495
1106	100	0.10016342852823712
1107	1	0.09974026171381922
1107	2	0.09768298168306444
1107	3	0.08176594422435102
1107	4	0.04654878168317822
1107	5	0.052224195930079986
1107	6	0.033426085694948426
1107	7	0.05633270195029638
1107	8	0.04500463624898998
1107	9	0.0650647647217113
1107	10-11	0.0675184672201965
1107	12-13	0.0910384541785163
1107	14-15	0.10450050967131119
1107	16-17	0.09528684952068289
1107	18-19	0.11347668142494172
1107	20-21	0.14230303729225113
1107	22-23	0.09677916264492836
1107	24-25	0.05668697662451194
1107	26-27	0.08781879719376917
1107	28-29	0.0766443065375455
1107	30-31	0.086081953571707
1107	32-33	0.07234145837286832
1107	34-35	0.09510196057601661
1107	36-37	0.11306158452016035
1107	38-39	0.11087337895355631
1107	40-41	0.11602786892538575
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2216	52-53	-0.6960412279954937
2216	54-55	-0.6272897760733116
2216	56-57	-0.535611958969767
2216	58-59	-0.49779315341406516
2216	60-61	-0.5405578949113448
2216	62-63	-0.49937456441506356
2216	64-65	-0.5231041973119446
2216	66-67	-0.46925561353734224
2216	68-69	-0.5828662983234878
2216	70-71	-0.5198493016849781
2216	72-73	-0.4974587889166955
2216	74-75	-0.5118046766693496
2216	76-77	-0.5625141034433767
2216	78-79	-0.5129482299242483
2216	80-81	-0.5141268129946397
2216	82-83	-0.5652242878613478
2216	84-85	-0.5721527659634447
2216	86-87	-0.5826341898993093
2216	88-89	-0.5992713712487614
2216	90-91	-0.662444053267734
2216	92-93	-0.7423435877505469
2216	94-95	-0.7130487629734503
2216	96-97	-0.6885280035434711
2216	98-99	-0.7483868854003788
2216	100	-0.7708217667115846
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	5.0
6	110.0
7	368.0
8	768.0
9	1301.0
10	1989.0
11	2940.0
12	4202.0
13	5840.0
14	8407.0
15	12615.0
16	18944.0
17	28510.0
18	38703.0
19	50152.0
20	62193.0
21	77588.0
22	94487.0
23	114522.0
24	139120.0
25	166645.0
26	200422.0
27	238841.0
28	284999.0
29	344456.0
30	408229.0
31	501147.0
32	636060.0
33	863237.0
34	1237882.0
35	3323534.0
36	3573523.0
37	6459626.0
38	1977530.0
39	444652.0
40	3148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.419649015077628	23.131783463648524	30.715365757397358	26.73320176387649
2	17.154876619360923	23.986714802094106	36.10781196571898	22.75059661282599
3	14.779809998689435	21.56188757798618	37.610211052392316	26.048091370932063
4	15.375495359219983	19.622656018665808	39.66780600557189	25.33404261654232
5	14.592271699704277	20.166075198845533	41.31882413158701	23.92282896986318
6	13.880608317416272	15.090584828075798	38.10163740795281	32.927169446555126
7	18.925961101802223	16.400051672328974	35.239810950548126	29.434176275320674
8	16.04292648251405	15.431772246004364	35.06876710151292	33.45653416996867
9	16.14822643639814	15.615190258031072	38.255219573403195	29.981363732167594
10-11	19.39235779383538	23.850402043073245	37.40914145393836	19.34809870915301
12-13	18.1546156120299	31.134915653831968	30.903576480087512	19.806892254050616
14-15	20.177699826438182	27.19816174048534	31.004365655344156	21.619772777732322
16-17	26.46845013715803	26.83413532182873	32.63542809235053	14.061986448662713
18-19	26.334598492640325	20.456828871046355	32.554019739111006	20.654552897202308
20-21	20.803142778072818	20.430847431845535	38.440843173290375	20.325166616791275
22-23	21.039432035765504	22.961337891314148	39.20764328462521	16.79158678829514
24-25	21.879745741104895	25.067758413021245	35.745902874039864	17.306592971833997
26-27	25.71354586352925	25.01514416556339	31.73977261830771	17.531537352599642
28-29	23.24762915656919	28.359061576025873	34.43532243841087	13.957986828994068
30-31	22.99080884089757	26.55806866488622	34.40707278932957	16.044049704886643
32-33	23.599651448652764	27.40418266774573	33.84393772149761	15.152228162103901
34-35	23.87740858578881	27.307492783031524	33.73492792362569	15.080170707553977
36-37	24.545221376307953	26.40783299990927	33.09325237689197	15.95369324689081
38-39	25.214476987404538	26.780529541282323	31.973926796837777	16.031066674475365
40-41	25.447031773171176	26.955980286094334	31.996386965580005	15.60060097515449
42-43	25.542592580349165	26.56594192511459	32.38195970607086	15.509505788465383
44-45	25.603571640077195	25.001253125604727	32.78197213414394	16.613203100174132
46-47	25.25309588582605	24.46823048935884	33.664269032776616	16.614404592038493
48-49	23.979674838967906	24.853915220268764	33.6529057190463	17.513504221717024
50-51	23.90560392665128	25.211703223630167	33.150635652418515	17.732057197300037
52-53	23.674865922706903	26.21662919953217	32.91376754134057	17.194737336420356
54-55	23.50725200696358	26.485120224394958	33.33219313411732	16.675434634524148
56-57	22.982451052411456	26.49598705395244	33.066674963010506	17.454886930625598
58-59	22.558435451833276	26.129638283364503	33.57091586722255	17.74101039757967
60-61	22.00165525064509	25.512534663448193	33.72667554740531	18.75913453850141
62-63	21.5584200927523	25.518759470232027	33.86143750824964	19.061382928766037
64-65	21.182769738215786	25.43104796352101	34.13896635703426	19.24721594122894
66-67	20.269254096802154	24.8015550463867	35.63448766909266	19.29470318771849
68-69	19.484012299048462	24.138614005768968	37.026300447319755	19.351073247862814
70-71	18.67003240266055	23.516577822149305	38.64283195788036	19.17055781730978
72-73	18.53945554114806	23.11654734746761	39.62659611149586	18.717400999888472
74-75	18.916722641299316	22.84994226700193	40.07162408491733	18.161711006781427
76-77	19.28741641278667	22.733933186121778	40.1838340778547	17.794816323236855
78-79	18.90417009647863	22.895500594683092	40.36584322264077	17.834486086197515
80-81	18.051090515221585	22.25661712901369	41.03017964925979	18.66211270650493
82-83	17.789789110518985	21.655700042285368	41.43846963166903	19.116041215526618
84-85	17.990937006727062	21.29427363273742	41.481951299499265	19.232838061036258
86-87	18.430585628499223	21.496641462247823	41.18951118244607	18.88326172680689
88-89	18.53092050787385	22.152777507508624	40.63349281740252	18.682809167215005
90-91	18.630628537914507	22.524200117102293	40.099088684942735	18.746082660040468
92-93	18.12401131663453	22.76875207618671	39.87156262665339	19.23567398052536
94-95	17.57116413651208	23.627275697815563	40.04174280880698	18.759817356865376
96-97	16.66803506661232	24.994505690566587	40.61316165048446	17.724297592336637
98-99	16.377674478362568	26.541263145999398	40.136994812287426	16.944067563350607
100	16.183265460553876	28.11785232204244	39.41371029050869	16.285171926894993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	312492.0
1	647720.5
2	850281.0
3	474918.0
4	146287.5
5	45751.5
6	26513.5
7	20399.0
8	18869.0
9	20154.5
10	23848.0
11	30605.5
12	41483.5
13	56840.0
14	77804.0
15	103614.0
16	133256.5
17	164937.5
18	196643.0
19	224811.5
20	247067.5
21	263219.5
22	274922.5
23	283994.0
24	290155.5
25	293707.0
26	293443.5
27	288832.5
28	279722.5
29	266419.5
30	250532.5
31	232628.0
32	213858.5
33	195017.0
34	176381.5
35	158562.5
36	141534.0
37	125733.0
38	111006.0
39	97660.5
40	87050.5
41	80595.5
42	80296.0
43	92738.5
44	126758.5
45	185825.0
46	265161.5
47	356533.0
48	458482.0
49	588712.0
50	772730.5
51	1021336.5
52	1308793.0
53	1554794.0
54	1657884.0
55	1549895.5
56	1253069.0
57	871650.0
58	524933.5
59	281977.0
60	139126.5
61	64795.5
62	29104.5
63	12912.0
64	5985.0
65	3041.5
66	1830.5
67	1297.5
68	974.0
69	711.5
70	544.0
71	433.0
72	326.0
73	262.5
74	221.5
75	160.0
76	111.5
77	73.5
78	49.5
79	30.5
80	23.0
81	17.5
82	11.5
83	9.0
84	8.5
85	7.0
86	4.5
87	2.0
88	1.5
89	3.0
90	4.5
91	3.0
92	2.5
93	2.5
94	1.0
95	1.5
96	1.5
97	0.5
98	0.0
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059695137948003664
2	0.006128469507347482
3	0.0
4	0.0
5	4.2669527556895247E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.205678376326009E-5
18-19	0.0
20-21	9.987482823756797E-4
22-23	0.0
24-25	3.5167193041397175E-5
26-27	0.0
28-29	9.377918144372581E-6
30-31	2.789930647950843E-4
32-33	6.494208314978012E-4
34-35	2.180365968566625E-4
36-37	0.0
38-39	2.2507003546494195E-4
40-41	1.0784605866028468E-4
42-43	2.7195962618680483E-4
44-45	1.0315709958809839E-4
46-47	2.086586787122899E-4
48-49	5.790864454150069E-4
50-51	1.1253501773247097E-4
52-53	1.8755836288745162E-5
54-55	5.861198840232863E-5
56-57	1.4770221077386816E-4
58-59	4.688959072186291E-6
60-61	7.267886561888751E-5
62-63	0.0
64-65	3.7511672577490325E-5
66-67	0.0
68-69	2.1100315824838305E-5
70-71	9.635810893342826E-4
72-73	0.001015159639128332
74-75	3.2822713505304037E-4
76-77	8.440126329935322E-5
78-79	9.84681405159121E-4
80-81	2.602372285063391E-4
82-83	7.92434083199483E-4
84-85	3.587053690222512E-4
86-87	3.6339432809443753E-4
88-89	0.0010221930777366114
90-91	1.9693628103182418E-4
92-93	1.711470061347996E-4
94-95	0.002937632858724711
96-97	3.9387256206364836E-4
98-99	0.0015942460845433388
100	0.001294152703923416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.1326695E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.92832375724484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.22596418495992	79.92155552211118
2	4.162390718963606	6.986849517306572
3	0.41051624259842007	1.0336182034922359
4	0.08465444587789586	0.2841962296452082
5	0.03481520802954427	0.1460991025589711
6	0.017525306497291636	0.08825217585897834
7	0.009471264288203449	0.0556435134899539
8	0.008581372460537397	0.05761761649195899
9	0.004734316887937157	0.03576089524861634
>10	0.03266425729715186	0.5037459647256013
>50	0.003726928357074864	0.21197814449229235
>100	0.0035892240113495196	0.5924022211623152
>500	3.656162772580792E-4	0.20467193586280363
>1k	5.033822880787017E-4	1.0235288214126017
>5k	1.732973862402428E-4	1.026522141453352
>10k+	3.24233819417166E-4	7.827557994687163
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74308	0.34842717073601887	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59615	0.27953229508838573	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56386	0.2643916462442962	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55596	0.260687368577269	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52524	0.24628288630751272	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52025	0.24394309573049175	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47422	0.22235981712121827	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46817	0.21952299688254556	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44888	0.2104779948322982	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44555	0.20891657146126016	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43806	0.20540454111619266	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42917	0.20123605650101906	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42231	0.19801943057749924	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41269	0.193508651950056	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38861	0.18221763850423145	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38623	0.1811016662450511	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37007	0.17352430838439806	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35867	0.16817889504210567	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35150	0.1648169113873481	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34608	0.16227549557022314	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34133	0.16004824001093465	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33495	0.1570566841228798	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32516	0.1524661931912094	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31517	0.14778192307809532	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31037	0.1455312227234459	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30227	0.141733165874975	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29931	0.14034523398960785	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29682	0.13917768318063348	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29681	0.1391729942215613	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28561	0.13392136006071265	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27156	0.1273333725642909	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21972	0.10302580873407718	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0022178776411441153	0.0	0.0	9.377918144372581E-6	0.0
2	0.005561105459612941	0.0	0.0	1.4066877216558872E-5	0.0
3	0.009551409630043474	0.0	0.0	2.8133754433117744E-5	0.0
4	0.016537958647601048	0.0	0.0	3.7511672577490325E-5	0.0
5	0.029240348774153706	0.0	0.0	3.7511672577490325E-5	0.0
6	0.05409652081581323	0.0	0.0	3.7511672577490325E-5	0.0
7	0.08744439773720213	0.0	0.0	4.220063164967662E-5	0.0
8	0.23364614160797068	0.0	0.0	4.220063164967662E-5	0.0
9	0.7847535682392419	0.0	0.0	4.220063164967662E-5	0.0
10-11	1.8913994878250007	0.0	0.0	4.220063164967662E-5	0.0
12-13	3.122260622192046	2.3444795360931453E-6	0.0	4.454511118576976E-5	0.0
14-15	4.4833247720755605	4.688959072186291E-6	0.0	5.1578549794049195E-5	2.3444795360931453E-6
16-17	5.844039594508198	4.688959072186291E-6	0.0	5.626750886623548E-5	4.688959072186291E-6
18-19	7.297844321400948	4.688959072186291E-6	0.0	6.564542701060807E-5	4.688959072186291E-6
20-21	9.17885542040152	4.688959072186291E-6	0.0	6.564542701060807E-5	4.688959072186291E-6
22-23	11.42527006645896	4.688959072186291E-6	0.0	6.564542701060807E-5	4.688959072186291E-6
24-25	14.050151699548383	4.688959072186291E-6	0.0	6.564542701060807E-5	4.688959072186291E-6
26-27	17.18056642156696	4.688959072186291E-6	2.3444795360931453E-6	7.033438608279436E-5	4.688959072186291E-6
28-29	21.204992147165793	4.688959072186291E-6	4.688959072186291E-6	7.502334515498065E-5	4.688959072186291E-6
30-31	26.00989979928911	4.688959072186291E-6	4.688959072186291E-6	8.909022237153952E-5	4.688959072186291E-6
32-33	31.128330010815084	4.688959072186291E-6	4.688959072186291E-6	8.909022237153952E-5	4.688959072186291E-6
34-35	35.434067491470195	4.688959072186291E-6	4.688959072186291E-6	8.909022237153952E-5	4.688959072186291E-6
36-37	38.3709008826731	4.688959072186291E-6	4.688959072186291E-6	1.0315709958809839E-4	4.688959072186291E-6
38-39	40.30742925708836	4.688959072186291E-6	4.688959072186291E-6	1.3363533355730927E-4	4.688959072186291E-6
40-41	41.91642680687279	4.688959072186291E-6	4.688959072186291E-6	1.6411356752652016E-4	4.688959072186291E-6
42-43	43.14258960424951	4.688959072186291E-6	4.688959072186291E-6	1.8286940381526532E-4	4.688959072186291E-6
44-45	44.1012801092715	4.688959072186291E-6	4.688959072186291E-6	1.9928076056791732E-4	4.688959072186291E-6
46-47	44.88055931779397	4.688959072186291E-6	4.688959072186291E-6	2.1100315824838307E-4	4.688959072186291E-6
48-49	45.54614299121359	4.688959072186291E-6	4.688959072186291E-6	2.3210347407322138E-4	4.688959072186291E-6
50-51	46.17679626402497	4.688959072186291E-6	4.688959072186291E-6	2.8368202386727057E-4	4.688959072186291E-6
52-53	46.835852906416115	4.688959072186291E-6	4.688959072186291E-6	3.422940122695992E-4	4.688959072186291E-6
54-55	47.468670133839304	4.688959072186291E-6	4.688959072186291E-6	3.798056848470895E-4	4.688959072186291E-6
56-57	48.095546450118036	4.688959072186291E-6	4.688959072186291E-6	3.985615211358347E-4	4.688959072186291E-6
58-59	48.68549252474422	4.688959072186291E-6	9.377918144372581E-6	4.2903975510504553E-4	4.688959072186291E-6
60-61	49.25365604000057	4.688959072186291E-6	9.377918144372581E-6	4.5717350953816335E-4	4.688959072186291E-6
62-63	49.803734240115496	7.033438608279436E-6	9.377918144372581E-6	4.759293458269085E-4	4.688959072186291E-6
64-65	50.33242844238172	9.377918144372581E-6	9.377918144372581E-6	5.110965388683056E-4	4.688959072186291E-6
66-67	50.80633450236898	9.377918144372581E-6	9.377918144372581E-6	5.579861295901686E-4	4.688959072186291E-6
68-69	51.21982098023159	9.377918144372581E-6	9.377918144372581E-6	5.908088430954725E-4	4.688959072186291E-6
70-71	51.60369199259426	9.377918144372581E-6	9.377918144372581E-6	5.954978021676589E-4	4.688959072186291E-6
72-73	51.975425634398576	9.377918144372581E-6	9.377918144372581E-6	6.119091589203109E-4	4.688959072186291E-6
74-75	52.33287436238948	9.377918144372581E-6	9.377918144372581E-6	6.28320515672963E-4	4.688959072186291E-6
76-77	52.677601944417546	9.377918144372581E-6	9.377918144372581E-6	6.634877087143601E-4	4.688959072186291E-6
78-79	52.997843782170655	9.377918144372581E-6	9.377918144372581E-6	7.314776152610613E-4	4.688959072186291E-6
80-81	53.305017490989584	9.377918144372581E-6	9.377918144372581E-6	8.487015920657186E-4	4.688959072186291E-6
82-83	53.60790783569606	9.377918144372581E-6	9.377918144372581E-6	8.88557744179302E-4	4.688959072186291E-6
84-85	53.90941962643532	9.377918144372581E-6	9.377918144372581E-6	8.979356623236746E-4	4.688959072186291E-6
86-87	54.21061725691674	9.377918144372581E-6	9.377918144372581E-6	9.073135804680472E-4	4.688959072186291E-6
88	54.43762852143757	9.377918144372581E-6	9.377918144372581E-6	9.190359781485129E-4	4.688959072186291E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCCTA	15865	0.0	60.344173	6
GCCCTAC	40395	0.0	55.206856	7
CCCCTAC	104900	0.0	54.888386	7
GCCCCTA	10990	0.0	54.610683	6
TGCTCTA	14810	0.0	53.028072	6
CCCCCTA	57185	0.0	51.983177	6
GCCTTTT	2515	0.0	51.4243	1
CTCCCTA	38005	0.0	51.295868	6
ATGCCCT	5140	0.0	50.01608	5
CGCCCTA	16275	0.0	49.843006	6
TCCCCTA	33695	0.0	49.083805	6
TTGCTCT	5405	0.0	48.69426	5
CCGCCTA	7165	0.0	46.63777	6
TTGCCCT	7115	0.0	46.503235	5
CCGCCCT	9240	0.0	46.388283	5
GTCCCTA	4225	0.0	46.38673	6
CCCGGAA	8945	0.0	46.131603	6
CTGCCCT	7975	0.0	45.967335	5
CACCCTA	21590	0.0	45.88834	6
ACCCTAC	37690	0.0	45.502174	7
>>END_MODULE