##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248493_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22770588
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26821775529029	34.0	31.0	34.0	30.0	34.0
2	32.55532825941956	34.0	31.0	34.0	31.0	34.0
3	32.67683017232581	34.0	31.0	34.0	31.0	34.0
4	36.16646733057574	37.0	35.0	37.0	35.0	37.0
5	36.07665897780066	37.0	35.0	37.0	35.0	37.0
6	36.06543629879035	37.0	35.0	37.0	35.0	37.0
7	36.0517782412997	37.0	35.0	37.0	35.0	37.0
8	36.04556237195104	37.0	35.0	37.0	35.0	37.0
9	37.76525090173341	39.0	38.0	39.0	35.0	39.0
10-11	37.77874018887874	39.0	38.0	39.0	35.0	39.0
12-13	37.776098491615585	39.0	38.0	39.0	35.0	39.0
14-15	39.18306174175213	41.0	39.0	41.0	36.0	41.0
16-17	39.07836036118171	41.0	39.0	41.0	36.0	41.0
18-19	38.89951144432459	40.0	38.0	41.0	35.0	41.0
20-21	38.70064468690927	40.0	38.0	41.0	35.0	41.0
22-23	38.53118487761493	40.0	37.5	41.0	34.0	41.0
24-25	38.37773192330387	40.0	37.0	41.0	34.0	41.0
26-27	38.20982466504598	40.0	37.0	41.0	34.0	41.0
28-29	38.0656269174955	40.0	37.0	41.0	33.0	41.0
30-31	38.003145856400366	40.0	37.0	41.0	33.0	41.0
32-33	37.937233307282185	40.0	36.0	41.0	33.0	41.0
34-35	37.90067026376306	40.0	36.0	41.0	33.0	41.0
36-37	37.809661173440055	40.0	36.0	41.0	33.0	41.0
38-39	37.72979999462464	40.0	36.0	41.0	33.0	41.0
40-41	37.587431031644854	40.0	35.5	41.0	33.0	41.0
42-43	37.428463002360765	40.0	35.0	41.0	32.5	41.0
44-45	37.18671046175882	39.0	35.0	41.0	32.0	41.0
46-47	36.88307699827514	39.0	35.0	41.0	31.5	41.0
48-49	36.53299938938775	38.0	35.0	40.5	31.0	41.0
50-51	36.14598795165061	37.0	35.0	40.0	31.0	41.0
52-53	35.74273262069473	36.5	35.0	39.5	31.0	41.0
54-55	35.40917164721438	36.0	35.0	39.0	31.0	41.0
56-57	35.08474862397054	35.0	35.0	38.5	30.5	41.0
58-59	34.762824504136645	35.0	34.0	37.0	30.0	40.0
60-61	34.47147192685581	35.0	34.0	37.0	30.0	40.0
62-63	34.21994399090617	35.0	34.0	36.0	30.0	40.0
64-65	34.00909776682095	35.0	34.0	35.5	29.0	39.0
66-67	33.826214983996024	35.0	34.0	35.0	29.0	39.0
68-69	33.66919541120326	35.0	34.0	35.0	29.0	38.5
70-71	33.570464737230324	35.0	34.0	35.0	29.0	37.5
72-73	33.463077194141846	35.0	34.0	35.0	29.0	37.0
74-75	33.35909404271862	35.0	34.0	35.0	29.0	36.5
76-77	33.24497338408652	35.0	34.0	35.0	29.0	36.0
78-79	33.10317008502372	35.0	33.0	35.0	29.0	36.0
80-81	32.968037847770994	35.0	33.0	35.0	29.0	36.0
82-83	32.85731418090741	35.0	33.0	35.0	29.0	35.5
84-85	32.74105556694452	35.0	33.0	35.0	27.0	35.0
86-87	32.64535002346009	35.0	33.0	35.0	27.0	35.0
88-89	32.52906819973204	35.0	33.0	35.0	27.0	35.0
90-91	32.430908854878936	35.0	33.0	35.0	27.0	35.0
92-93	32.258557266944536	35.0	33.0	35.0	26.0	35.0
94-95	31.967459975122292	35.0	33.0	35.0	24.5	35.0
96-97	31.711730193352935	35.0	33.0	35.0	24.0	35.0
98-99	31.429709303071135	35.0	32.5	35.0	21.5	35.0
100	30.16493702314582	34.0	30.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2945964621011399
1101	2	0.27618364129462236
1101	3	0.2590131646015905
1101	4	0.1767664332735066
1101	5	0.20191311159928205
1101	6	0.1942264391343116
1101	7	0.20593642694159087
1101	8	0.2123863349546582
1101	9	0.28579140043664353
1101	10-11	0.2939073714666236
1101	12-13	0.2843075228122487
1101	14-15	0.38499279522658014
1101	16-17	0.39397259537890505
1101	18-19	0.396893251247306
1101	20-21	0.385491868075448
1101	22-23	0.38845938223332865
1101	24-25	0.39384312797369603
1101	26-27	0.40454368563393217
1101	28-29	0.43726257308012606
1101	30-31	0.40989637459235695
1101	32-33	0.421290902805076
1101	34-35	0.47061363794398403
1101	36-37	0.4950771988974836
1101	38-39	0.45521080798985736
1101	40-41	0.4585429497985345
1101	42-43	0.4508486338148785
1101	44-45	0.44702396074816875
1101	46-47	0.43947888099677357
1101	48-49	0.41849123965210566
1101	50-51	0.3745138122718501
1101	52-53	0.3959394007163013
1101	54-55	0.38424522098086555
1101	56-57	0.38768872993305337
1101	58-59	0.4072659412778208
1101	60-61	0.39744948865023844
1101	62-63	0.36871475995391023
1101	64-65	0.3699222218376974
1101	66-67	0.3876417790045039
1101	68-69	0.4071527923793994
1101	70-71	0.396881830304622
1101	72-73	0.40397025134732445
1101	74-75	0.3645625276250044
1101	76-77	0.38718764953488005
1101	78-79	0.39020129941054904
1101	80-81	0.4279056338215099
1101	82-83	0.4146024361026761
1101	84-85	0.38538874597934125
1101	86-87	0.3865687797964483
1101	88-89	0.4402536051285537
1101	90-91	0.45604609239017435
1101	92-93	0.4502090070673148
1101	94-95	0.45323681296783747
1101	96-97	0.4570331862094825
1101	98-99	0.4504896703528267
1101	100	0.4605855168991866
1102	1	0.2502088075072919
1102	2	0.2642429222907765
1102	3	0.2476843345009243
1102	4	0.1739671480413989
1102	5	0.19626644059634657
1102	6	0.1872587506875476
1102	7	0.20737756680346564
1102	8	0.20485578856963826
1102	9	0.28741838312702583
1102	10-11	0.29675269081197797
1102	12-13	0.2974777631920489
1102	14-15	0.3853064617402566
1102	16-17	0.3977479511591966
1102	18-19	0.40970742358367573
1102	20-21	0.4058026369353769
1102	22-23	0.3923166731502903
1102	24-25	0.38308213174957473
1102	26-27	0.4074322288014258
1102	28-29	0.4214171066703898
1102	30-31	0.44437690634877924
1102	32-33	0.4494646636062569
1102	34-35	0.45260577669584734
1102	36-37	0.49204202032446887
1102	38-39	0.5011883351719462
1102	40-41	0.47046991501188273
1102	42-43	0.47333996756033514
1102	44-45	0.4730038300613373
1102	46-47	0.4596329924658704
1102	48-49	0.4637416934261793
1102	50-51	0.4358638457516193
1102	52-53	0.44382724734750667
1102	54-55	0.4323376039267046
1102	56-57	0.39417745565230433
1102	58-59	0.3946676884204834
1102	60-61	0.39448439505398625
1102	62-63	0.38681751160250144
1102	64-65	0.3911520521436671
1102	66-67	0.4149851225464687
1102	68-69	0.3962983049589468
1102	70-71	0.38582437688653215
1102	72-73	0.3535715991348951
1102	74-75	0.3696776680088618
1102	76-77	0.3317704875806484
1102	78-79	0.37659225851223965
1102	80-81	0.3866639224840256
1102	82-83	0.3644295839488194
1102	84-85	0.3240713903032457
1102	86-87	0.3257890571707307
1102	88-89	0.3624105755465976
1102	90-91	0.4256489463036601
1102	92-93	0.4024466824156079
1102	94-95	0.4272105542445601
1102	96-97	0.4212107832232057
1102	98-99	0.3743308660611824
1102	100	0.3444421383608365
1103	1	0.21570014244507263
1103	2	0.22945994773346
1103	3	0.2249264797123658
1103	4	0.1407422593568768
1103	5	0.13862244163532011
1103	6	0.16482822380228157
1103	7	0.1635800766259905
1103	8	0.17044201974689344
1103	9	0.24659963755220105
1103	10-11	0.24621833913689528
1103	12-13	0.2312285991103522
1103	14-15	0.31745663181727934
1103	16-17	0.33303642073699535
1103	18-19	0.3369781332027273
1103	20-21	0.3265110611793105
1103	22-23	0.3086597051365416
1103	24-25	0.33404035852038305
1103	26-27	0.33921616411478794
1103	28-29	0.3632517457416782
1103	30-31	0.3614243494447038
1103	32-33	0.3694685160103077
1103	34-35	0.4432453443015092
1103	36-37	0.43045476132211746
1103	38-39	0.4202126520409024
1103	40-41	0.4291878993123035
1103	42-43	0.42166811188733533
1103	44-45	0.43243612532397435
1103	46-47	0.42994007948504986
1103	48-49	0.4368043192978135
1103	50-51	0.44649911397663544
1103	52-53	0.4133714231029586
1103	54-55	0.3860863946349582
1103	56-57	0.3878343414173102
1103	58-59	0.3975420954351634
1103	60-61	0.3623449946900763
1103	62-63	0.36256172182257274
1103	64-65	0.3367066541436401
1103	66-67	0.37240308882322637
1103	68-69	0.365622132578018
1103	70-71	0.3451889923266833
1103	72-73	0.336216034210544
1103	74-75	0.32915403944078037
1103	76-77	0.32546209558110917
1103	78-79	0.3588877139164097
1103	80-81	0.38031128963724825
1103	82-83	0.32373759641528466
1103	84-85	0.34603393174718633
1103	86-87	0.3362618750981241
1103	88-89	0.3515728470468247
1103	90-91	0.3998877185337122
1103	92-93	0.40074853455247705
1103	94-95	0.40351130153216985
1103	96-97	0.3665346702707204
1103	98-99	0.37067178886112373
1103	100	0.38957602065274344
1104	1	0.16163446707242457
1104	2	0.1852377064813453
1104	3	0.17421553233163678
1104	4	0.12249898753270827
1104	5	0.1425652085604341
1104	6	0.11592936035163603
1104	7	0.14592616848890572
1104	8	0.14347527751395006
1104	9	0.2290960178612238
1104	10-11	0.20039896111188682
1104	12-13	0.20428316492808563
1104	14-15	0.2616091539682941
1104	16-17	0.2712260499429746
1104	18-19	0.28003518207419376
1104	20-21	0.27659173492298805
1104	22-23	0.24901623789228466
1104	24-25	0.2637810906071678
1104	26-27	0.3049406102971517
1104	28-29	0.26703454948357574
1104	30-31	0.27086503959358765
1104	32-33	0.2838123166901383
1104	34-35	0.32955836636699587
1104	36-37	0.31833359578152454
1104	38-39	0.33611366376786833
1104	40-41	0.3265694572199749
1104	42-43	0.3195898817159062
1104	44-45	0.35157223594504217
1104	46-47	0.3609491981407942
1104	48-49	0.34114203941035015
1104	50-51	0.3004706645237789
1104	52-53	0.2749551528144991
1104	54-55	0.26618208449583847
1104	56-57	0.2646755164598673
1104	58-59	0.2703531510006272
1104	60-61	0.24657245925146754
1104	62-63	0.2604289601952843
1104	64-65	0.2601856291258855
1104	66-67	0.24431781197800717
1104	68-69	0.24088932750325398
1104	70-71	0.23223495412052841
1104	72-73	0.23123005947446273
1104	74-75	0.22401191836247847
1104	76-77	0.19472350440805997
1104	78-79	0.25071564291146586
1104	80-81	0.2814095390617055
1104	82-83	0.23670893083328792
1104	84-85	0.23891508095394443
1104	86-87	0.23145439927243672
1104	88-89	0.2392510784190236
1104	90-91	0.2546920064049729
1104	92-93	0.25432574153251153
1104	94-95	0.21729827174181793
1104	96-97	0.2511327511865922
1104	98-99	0.23722099656366424
1104	100	0.20203859681970826
1105	1	0.15631186686438525
1105	2	0.1629252929691276
1105	3	0.15840487031969985
1105	4	0.10191142754862881
1105	5	0.11948356839572227
1105	6	0.11942628291585322
1105	7	0.1402836802136349
1105	8	0.12471125347025236
1105	9	0.17784570969750746
1105	10-11	0.1710994506876844
1105	12-13	0.18305459990590833
1105	14-15	0.22087485028625053
1105	16-17	0.24835755428557604
1105	18-19	0.2561590304521957
1105	20-21	0.24235484481643255
1105	22-23	0.21436143802326058
1105	24-25	0.2023230817241526
1105	26-27	0.23127809884526584
1105	28-29	0.2825282203797812
1105	30-31	0.2575491529906344
1105	32-33	0.23037652470426195
1105	34-35	0.27870109579647107
1105	36-37	0.3182747369493413
1105	38-39	0.30369013976140735
1105	40-41	0.2869203749789122
1105	42-43	0.28840355256608774
1105	44-45	0.3266432525204408
1105	46-47	0.3244772939438576
1105	48-49	0.2936944387459164
1105	50-51	0.2855334413928432
1105	52-53	0.28490623824851014
1105	54-55	0.28014497060578236
1105	56-57	0.270868080407503
1105	58-59	0.26012383368135517
1105	60-61	0.26962436114864374
1105	62-63	0.2633472757160149
1105	64-65	0.2772596650929984
1105	66-67	0.28008139902817675
1105	68-69	0.283166657992453
1105	70-71	0.2715702332929766
1105	72-73	0.23518970602037825
1105	74-75	0.22030194382134027
1105	76-77	0.24762855857310484
1105	78-79	0.27651193820464925
1105	80-81	0.26662457494024494
1105	82-83	0.29234462437683817
1105	84-85	0.24077132313669836
1105	86-87	0.2520400806394818
1105	88-89	0.2610958108213808
1105	90-91	0.2649202588745041
1105	92-93	0.28234243661601965
1105	94-95	0.2960716948726443
1105	96-97	0.2812111839478284
1105	98-99	0.2695717116560381
1105	100	0.23769809818293197
1106	1	0.12740520317468196
1106	2	0.13097082344057753
1106	3	0.12526673211041128
1106	4	0.08725973123900843
1106	5	0.1001329720902504
1106	6	0.08395065531341572
1106	7	0.07691933200831613
1106	8	0.08537407813656017
1106	9	0.13264649801138262
1106	10-11	0.12395941788676623
1106	12-13	0.14393623417504386
1106	14-15	0.17040679650152413
1106	16-17	0.16773997256614592
1106	18-19	0.19059493266048833
1106	20-21	0.1789788070413465
1106	22-23	0.17727197315442567
1106	24-25	0.20022049629647398
1106	26-27	0.18630008679804178
1106	28-29	0.21260765543666338
1106	30-31	0.20716370233995463
1106	32-33	0.23004659074393885
1106	34-35	0.24868892480787252
1106	36-37	0.24759826007857555
1106	38-39	0.2492463765899089
1106	40-41	0.2507013381236476
1106	42-43	0.23199695189676817
1106	44-45	0.22650686504573514
1106	46-47	0.2442657122574161
1106	48-49	0.23549931684554792
1106	50-51	0.21982032646319283
1106	52-53	0.18746548830408472
1106	54-55	0.20492967308609167
1106	56-57	0.20997686988361153
1106	58-59	0.17060033219700443
1106	60-61	0.1666851645381371
1106	62-63	0.1801927389404483
1106	64-65	0.1755016836329304
1106	66-67	0.18762188512094724
1106	68-69	0.19215025772035688
1106	70-71	0.14636460395250595
1106	72-73	0.17067868636200245
1106	74-75	0.16062293173072106
1106	76-77	0.14166627148485134
1106	78-79	0.16707577822879216
1106	80-81	0.1986130394624226
1106	82-83	0.16652644452292975
1106	84-85	0.1787242003819074
1106	86-87	0.17337663960542926
1106	88-89	0.19764274129293824
1106	90-91	0.16736179444139765
1106	92-93	0.1923519380044567
1106	94-95	0.21196836779460426
1106	96-97	0.22498245901217118
1106	98-99	0.2515298505817647
1106	100	0.23282117745915798
1107	1	0.11740791801193495
1107	2	0.11189252869840516
1107	3	0.10527607007281148
1107	4	0.07751120049590554
1107	5	0.07212074546724523
1107	6	0.054594013676315
1107	7	0.06505177079494473
1107	8	0.08071552143983496
1107	9	0.09085007498596553
1107	10-11	0.09913622630956098
1107	12-13	0.09016938271828678
1107	14-15	0.12795275090701352
1107	16-17	0.13595785286583606
1107	18-19	0.11914590794437885
1107	20-21	0.13791522581800564
1107	22-23	0.1148567559858833
1107	24-25	0.10879238131681745
1107	26-27	0.13630107701770555
1107	28-29	0.12272232899801594
1107	30-31	0.12503238305987452
1107	32-33	0.12414313737066607
1107	34-35	0.1685049973216124
1107	36-37	0.1843594585877284
1107	38-39	0.1648593142086554
1107	40-41	0.12356531110368252
1107	42-43	0.15074135566076308
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2216	60-61	-0.49523411128880923
2216	62-63	-0.4862740601402322
2216	64-65	-0.5065861153979654
2216	66-67	-0.4853350574075961
2216	68-69	-0.5525652710702502
2216	70-71	-0.4681777562023868
2216	72-73	-0.47641983783629627
2216	74-75	-0.49369638644016334
2216	76-77	-0.5180930216699906
2216	78-79	-0.4442410445860574
2216	80-81	-0.48136918319239896
2216	82-83	-0.5249113659028382
2216	84-85	-0.5474467091786721
2216	86-87	-0.5529867315428092
2216	88-89	-0.5672010926839945
2216	90-91	-0.6637968555332527
2216	92-93	-0.7279093275494333
2216	94-95	-0.7176760724027034
2216	96-97	-0.7289011820183724
2216	98-99	-0.7713704824268142
2216	100	-0.7801459397708044
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	4.0
6	76.0
7	279.0
8	603.0
9	1110.0
10	1748.0
11	2462.0
12	3694.0
13	5143.0
14	7495.0
15	11856.0
16	18445.0
17	27986.0
18	38365.0
19	49971.0
20	62879.0
21	78616.0
22	95945.0
23	117534.0
24	144309.0
25	174873.0
26	210246.0
27	253380.0
28	304363.0
29	369480.0
30	437130.0
31	538088.0
32	685259.0
33	932623.0
34	1328932.0
35	3244646.0
36	3866821.0
37	6916435.0
38	2283205.0
39	552532.0
40	4055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.313618275647464	23.34991607707647	30.743064906384976	26.593400740891088
2	17.14130956986675	24.005932024327677	36.048813636857325	22.803944768948252
3	14.965665357433897	22.092472974347434	36.807780282178044	26.134081386040624
4	15.53126339996139	20.153313563971206	38.53705929772213	25.778363738345274
5	14.85285460291724	20.687569291941482	40.08747719912228	24.372098906018994
6	14.18542200139935	15.527978460635271	37.11797868373008	33.1686208542353
7	19.642338616815692	17.1191275341682	34.096405415617724	29.142128433398383
8	17.318446058573457	16.30794953560268	33.5132057195888	32.86039868623507
9	17.325200385690522	16.726348919931272	36.30762631162621	29.640824382751994
10-11	20.246615941582185	25.161699820839058	35.27841266110476	19.313271576474005
12-13	19.0048978972348	31.703487411040943	29.364467882867146	19.92714680885711
14-15	21.00374395250575	27.51856693380074	29.622539830767657	21.85514928292585
16-17	26.642772365477846	27.411277771712438	31.443797917679856	14.502151945129857
18-19	26.73151435527269	21.430959534290466	31.08072791093493	20.75679819950192
20-21	21.70400925852833	21.40515360815042	36.49080828001932	20.40002885330193
22-23	21.847556154456793	23.928312698820076	37.30223391684044	16.921897229882692
24-25	22.679981946004236	25.67732511893361	34.021817404100226	17.620875530961932
26-27	26.24558048303364	25.534830281940895	30.33153996725952	17.888049267765943
28-29	23.83821830496589	28.91858602145768	32.70449649385396	14.538699179722473
30-31	23.69607275474524	27.326858048112495	32.595809095954884	16.381260101187376
32-33	24.279941793817848	27.946066551609565	32.1318344685884	15.642157185984185
34-35	24.556725988571856	27.903717687823576	32.02977092657562	15.509785397028947
36-37	25.102944201528743	26.997012549697878	31.53700510500651	16.36303814376686
38-39	25.794491626608167	27.424684904974743	30.330776134253927	16.450047334163163
40-41	26.04429590573042	27.819422786066024	30.216097581573443	15.920183726630114
42-43	26.146210815348088	27.179868846755646	30.5314738164844	16.14244652141187
44-45	26.183067609503514	25.476829281092687	31.107291951528136	17.232811157875663
46-47	25.576779483062612	25.187471845489917	32.01188684376455	17.223861827682928
48-49	24.270929618109257	25.529953785503384	32.01158387168634	18.187532724701015
50-51	24.181822158242948	25.909721447891794	31.448421425042124	18.460034968823134
52-53	23.994797937435376	26.53684522401457	31.4016832287148	18.066673609835252
54-55	23.429134147181767	26.58071189142797	31.79111958461838	18.199034376771888
56-57	22.92300301861243	26.051502946029682	32.061288870720205	18.964205164637686
58-59	22.215627457666002	25.5841790295446	32.59436910456594	19.60582440822345
60-61	21.7030473514801	24.711304520550893	32.80415188434074	20.781496243628265
62-63	21.140380740277763	24.47620808035348	33.109116461990354	21.2742947173784
64-65	20.49530877205433	24.13843887359235	33.608696641245565	21.757555713107752
66-67	19.29789208781082	23.344300551219845	35.27463366339069	22.083173697578648
68-69	18.604485659603014	22.039572849825365	36.97118209932801	22.384759391243612
70-71	17.559234796051012	21.190989178934057	38.67715103818993	22.572624986825
72-73	17.03293904212026	20.11126289201395	39.997108087057775	22.85868997880801
74-75	16.753762129859833	19.23880940317	40.75274326829491	23.254685198675258
76-77	17.247276896051144	19.03416904149758	40.632090498750614	23.08646356370066
78-79	17.699462841379514	19.514712300007083	40.554323032709746	22.231501825903656
80-81	17.611950635247382	19.65282902729771	40.8457665592757	21.889453778179202
82-83	17.71940587596708	19.91743195193433	40.88721994079119	21.475942231307396
84-85	17.948424778477353	20.288943679499738	40.58821869629502	21.174412845727883
86-87	18.46363418797586	20.927416901361628	40.14285667546071	20.4660922352018
88-89	18.729803248695564	21.963057692957996	39.36944590711526	19.937693151231183
90-91	18.78039739608472	22.66960405644895	38.68763593898111	19.862362608485213
92-93	18.288651700075633	23.007332208620866	38.425922805018345	20.278093286285156
94-95	17.734784405398525	23.917917413108526	38.56668538796242	19.78061279353053
96-97	16.758856425538543	25.383531611306736	39.14006470340999	18.717547259744734
98-99	16.482831692470885	26.900622176111355	38.646053416825936	17.97049271459182
100	16.322393825785085	28.451033082023645	37.84007439160254	17.38649870058873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	299163.0
1	597906.5
2	726427.5
3	359311.0
4	105415.0
5	37988.5
6	23958.5
7	19306.0
8	18475.0
9	19754.0
10	23275.0
11	30134.5
12	40960.5
13	56587.0
14	77812.5
15	104296.5
16	134331.0
17	165120.0
18	196004.0
19	225858.0
20	251231.0
21	271286.5
22	286779.5
23	298689.5
24	308179.5
25	314730.5
26	316522.0
27	312947.0
28	305150.0
29	292914.5
30	276691.0
31	259056.5
32	241032.5
33	223074.0
34	205511.0
35	188666.0
36	172329.5
37	156529.0
38	142574.0
39	129164.5
40	116984.0
41	107816.5
42	102453.5
43	104668.0
44	123069.0
45	168269.0
46	245047.5
47	347568.0
48	465763.0
49	596970.0
50	756466.0
51	968815.0
52	1238377.0
53	1524356.0
54	1738169.0
55	1776939.0
56	1582099.5
57	1201703.5
58	771257.5
59	417954.5
60	194408.5
61	85061.5
62	36813.0
63	14008.0
64	6590.0
65	3712.5
66	2422.5
67	1784.0
68	1386.5
69	1031.0
70	778.5
71	584.5
72	415.5
73	326.0
74	258.5
75	198.0
76	138.0
77	101.0
78	73.5
79	45.0
80	31.5
81	21.5
82	19.0
83	14.5
84	12.0
85	12.5
86	10.0
87	7.0
88	5.0
89	2.5
90	1.5
91	3.0
92	3.0
93	1.5
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.058834668652386145
2	0.005687160999092338
3	0.0
4	0.0
5	3.6450529955572516E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.1418238299335967E-4
18-19	0.0
20-21	0.0010122707415372847
22-23	0.0
24-25	2.41539656332107E-5
26-27	0.0
28-29	8.783260230258436E-6
30-31	2.613019918501885E-4
32-33	6.785068527874643E-4
34-35	2.2177732081402556E-4
36-37	0.0
38-39	1.602944992022165E-4
40-41	8.124515712989053E-5
42-43	2.1738569069889633E-4
44-45	7.465771195719672E-5
46-47	1.5590286908708726E-4
48-49	5.950658806000091E-4
50-51	8.344097218745515E-5
52-53	1.9762335518081483E-5
54-55	3.9524671036162966E-5
56-57	1.1637819805092428E-4
58-59	0.0
60-61	5.050374632398601E-5
62-63	0.0
64-65	1.7566520460516872E-5
66-67	0.0
68-69	6.587445172693828E-6
70-71	8.256264616442931E-4
72-73	8.475846122199392E-4
74-75	3.31568073692256E-4
76-77	8.563678724501976E-5
78-79	9.617669952132988E-4
80-81	2.1738569069889633E-4
82-83	6.916817431328519E-4
84-85	2.6569362196531774E-4
86-87	3.227848134619976E-4
88-89	9.573753650981696E-4
90-91	1.3833634862657037E-4
92-93	1.207698281660535E-4
94-95	0.0027162232262074215
96-97	3.183931833468683E-4
98-99	0.0015238956499498387
100	0.0012120899117756643
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.2770588E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.1461733487477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.42298545431211	80.39755887599142
2	4.885091454128087	8.31893687555352
3	0.477136853380628	1.2187913168706888
4	0.09686765668782665	0.32991641152914664
5	0.03546705638192329	0.1509942065432524
6	0.01981157202737836	0.10121277276926152
7	0.009057512819064358	0.05398487896203908
8	0.008492101742711197	0.057845597366407195
9	0.005857952763958272	0.044890403535789006
>10	0.032473533605952024	0.5048420351120458
>50	0.002634789704050609	0.16099045055056696
>100	0.002987560750824955	0.4754829933946657
>500	2.9604845726688347E-4	0.17538981021368488
>1k	4.797960032067473E-4	0.9156253646790088
>5k	1.2880615533618207E-4	0.7904213901322742
>10k+	2.3185107960464835E-4	6.30311661679612
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73905	0.3245634236586249	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61105	0.2683505581849709	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59954	0.26329579192245717	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57811	0.25388452858573524	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53611	0.23543968210219252	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53235	0.2337884291789039	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50961	0.2238018622971001	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48206	0.2117029213299191	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47271	0.20759674717227328	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45473	0.19970059622527095	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44738	0.196472748090651	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44269	0.19441307356665538	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43272	0.19003461834187155	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42260	0.18559028866536076	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38089	0.1672727994551568	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35691	0.15674167043907694	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35001	0.15371144565963776	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33993	0.14928468250358753	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33934	0.14902557632679492	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33856	0.14868302917781484	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33077	0.14526194931812916	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31679	0.13912245041717852	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30636	0.13454198020709873	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29491	0.12951356372527578	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29182	0.12815655001970086	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29165	0.12808189230774364	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28039	0.12313691679810815	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25665	0.1127111869047914	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25269	0.11097210137920022	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24009	0.10543864743413742	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22856	0.10037509791139342	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0025207956860841714	0.0	0.0	4.391630115129219E-6	0.0
2	0.00657427028234844	0.0	0.0	8.783260230258438E-6	0.0
3	0.011409455039105709	4.391630115129219E-6	0.0	3.074141080590453E-5	0.0
4	0.01960423683393683	4.391630115129219E-6	0.0	3.074141080590453E-5	0.0
5	0.03433815587019536	4.391630115129219E-6	0.0	3.074141080590453E-5	0.0
6	0.0627520027150814	4.391630115129219E-6	0.0	3.513304092103375E-5	0.0
7	0.10060785430749526	4.391630115129219E-6	0.0	3.513304092103375E-5	0.0
8	0.26450788183423285	4.391630115129219E-6	0.0	3.513304092103375E-5	0.0
9	0.8694637134535129	4.391630115129219E-6	0.0	3.513304092103375E-5	0.0
10-11	2.085402889025088	4.391630115129219E-6	0.0	3.513304092103375E-5	0.0
12-13	3.4317690873858857	4.391630115129219E-6	0.0	3.513304092103375E-5	0.0
14-15	4.932986359421197	4.391630115129219E-6	0.0	4.6112116208856796E-5	0.0
16-17	6.4225240911653225	4.391630115129219E-6	0.0	5.050374632398601E-5	0.0
18-19	7.970435370399746	4.391630115129219E-6	0.0	5.4895376439115226E-5	0.0
20-21	9.96964593097025	4.391630115129219E-6	0.0	6.587445172693827E-5	0.0
22-23	12.345853782958965	4.391630115129219E-6	0.0	6.587445172693827E-5	0.0
24-25	14.994197778291891	4.391630115129219E-6	0.0	7.02660818420675E-5	0.0
26-27	18.10692372107387	4.391630115129219E-6	4.391630115129219E-6	7.02660818420675E-5	0.0
28-29	22.118337479910487	4.391630115129219E-6	4.391630115129219E-6	8.344097218745515E-5	0.0
30-31	26.950208751745894	4.391630115129219E-6	4.391630115129219E-6	8.344097218745515E-5	0.0
32-33	32.06081459117349	4.391630115129219E-6	4.391630115129219E-6	9.222423241771359E-5	0.0
34-35	36.32916945315598	4.391630115129219E-6	4.391630115129219E-6	9.222423241771359E-5	0.0
36-37	39.0987312229267	4.391630115129219E-6	4.391630115129219E-6	9.222423241771359E-5	0.0
38-39	40.93110375542345	8.783260230258438E-6	4.391630115129219E-6	1.0100749264797202E-4	0.0
40-41	42.531479643828256	8.783260230258438E-6	4.391630115129219E-6	1.1198656793579507E-4	0.0
42-43	43.790109855749	8.783260230258438E-6	4.391630115129219E-6	1.449237937992642E-4	0.0
44-45	44.7462006690385	8.783260230258438E-6	4.391630115129219E-6	1.5370705402952263E-4	0.0
46-47	45.520150819118065	8.783260230258438E-6	4.391630115129219E-6	1.646861293173457E-4	0.0
48-49	46.1827270336629	8.783260230258438E-6	4.391630115129219E-6	1.7346938954760412E-4	0.0
50-51	46.814568424846996	8.783260230258438E-6	4.391630115129219E-6	1.932317250656856E-4	0.0
52-53	47.477697984786346	8.783260230258438E-6	6.587445172693828E-6	2.3714802621697777E-4	0.0
54-55	48.095837490011235	8.783260230258438E-6	8.783260230258438E-6	2.7447688219557615E-4	0.0
56-57	48.701129281334325	8.783260230258438E-6	8.783260230258438E-6	2.876517725409638E-4	0.0
58-59	49.277467055308364	8.783260230258438E-6	8.783260230258438E-6	3.0302247794391607E-4	0.0
60-61	49.8277668543298	8.783260230258438E-6	8.783260230258438E-6	3.140015532317391E-4	0.0
62-63	50.35932537183493	8.783260230258438E-6	8.783260230258438E-6	3.2278481346199753E-4	0.0
64-65	50.87432524799096	1.3174890345387655E-5	8.783260230258438E-6	3.3156807369225604E-4	0.0
66-67	51.34498283487453	1.3174890345387655E-5	8.783260230258438E-6	3.6670111461328976E-4	0.0
68-69	51.76645855609877	1.3174890345387655E-5	8.783260230258438E-6	3.8207182001624197E-4	0.0
70-71	52.168997568266576	1.3174890345387655E-5	8.783260230258438E-6	3.974425254191943E-4	0.0
72-73	52.56301242638091	1.3174890345387655E-5	8.783260230258438E-6	4.040299705918881E-4	0.0
74-75	52.94522038693072	1.3174890345387655E-5	8.783260230258438E-6	4.1720486093727575E-4	0.0
76-77	53.31714973719607	1.3174890345387655E-5	8.783260230258438E-6	4.479462717431803E-4	0.0
78-79	53.66946606736725	1.3174890345387655E-5	8.783260230258438E-6	5.291914288730708E-4	0.0
80-81	54.01034439690359	1.3174890345387655E-5	8.783260230258438E-6	6.411779968088659E-4	0.0
82-83	54.346569794332936	1.3174890345387655E-5	8.783260230258438E-6	6.587445172693828E-4	0.0
84-85	54.67924016718409	1.3174890345387655E-5	8.783260230258438E-6	6.63136147384512E-4	0.0
86-87	55.011087109388654	1.3174890345387655E-5	8.783260230258438E-6	6.719194076147704E-4	0.0
88	55.26119044444526	1.3174890345387655E-5	8.783260230258438E-6	6.719194076147704E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCCTA	14890	0.0	60.224335	6
GCCCTAC	36360	0.0	57.02955	7
CCCCTAC	97570	0.0	55.756187	7
CCCCCTA	53475	0.0	54.860695	6
CTCCCTA	41050	0.0	51.819084	6
GCCCCTA	9960	0.0	50.585236	6
CGCCCTA	15510	0.0	49.9383	6
TCCCCTA	33155	0.0	49.21744	6
TGCTCTA	15005	0.0	49.144543	6
ATGCCCT	5140	0.0	46.45055	5
CCGCCCT	8910	0.0	45.891453	5
TTGCCCT	7020	0.0	45.59322	5
CTGCCCT	7790	0.0	45.189194	5
GCTGCGT	2135	0.0	44.68774	5
CCGCCTA	6915	0.0	44.586155	6
TCCCCCT	23305	0.0	44.52872	5
GGCCCTA	5200	0.0	43.56443	6
CCCGGAA	9615	0.0	43.16182	6
CTGCGTC	3665	0.0	42.831257	6
CCACACT	23835	0.0	42.670765	8
>>END_MODULE