FastQCFastQC Report
Thu 26 May 2016
SRR1248492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21825607
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5708042.6152949606395826No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1246630.5711776996626028No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1073620.4919084266476529No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA988630.45296792891029336No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA465520.21329074604889567No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA386450.17706265855515496No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340910.1561972594851543No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA329730.15107483608588754No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA306450.14040846607381868No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA272850.12501370523165747No Hit
CCAGCTCACCACCACCATTCCGCCACCATCCCCCGCCGCCAGATCGGAAG253250.11603342807373009No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA238510.10927989310904387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCA47450.078.537922
AGCTCAC48400.077.28733
CCAGCTC50850.073.6559451
GCTCACC72800.052.028834
TTGCTGA74350.044.9404079
GCTTGCT15350.041.330137
TACCCGA161000.040.7179376
CTACCCG160200.039.1025475
ACCCGAC174350.038.085677
CCCGACG186000.037.165948
CTTGCTG49500.036.3612638
CATTCCG49350.035.47345416-17
TGACGCC13750.034.17733
CTGCTGA79600.033.3567549
ATTCCGC51050.033.27950716-17
CCTGCTG56600.032.298228
GTTGCTG22150.032.0367628
AGTTGCC6400.031.573953
TCACCAC123850.031.1139586
TTGCGCC13950.030.9923153
Produced by FastQC (version 0.11.5)