##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248492_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21825607
Sequences flagged as poor quality	0
Sequence length	100
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83428974048694	33.0	31.0	34.0	26.0	34.0
2	31.051253144986987	34.0	31.0	34.0	27.0	34.0
3	31.169219806807664	34.0	31.0	34.0	27.0	34.0
4	34.51366488913688	37.0	35.0	37.0	30.0	37.0
5	34.45414480339539	37.0	35.0	37.0	30.0	37.0
6	34.40463506925604	37.0	35.0	37.0	30.0	37.0
7	34.41616730292999	37.0	35.0	37.0	30.0	37.0
8	34.38382744635693	37.0	35.0	37.0	30.0	37.0
9	35.88672773224589	39.0	37.0	39.0	30.0	39.0
10-11	36.048424082775796	39.0	37.0	39.0	30.0	39.0
12-13	36.04994665211373	39.0	37.0	39.0	30.0	39.0
14-15	37.15840111571697	40.0	37.0	41.0	30.5	41.0
16-17	36.99335070039518	40.0	37.0	41.0	30.0	41.0
18-19	36.75359567319251	40.0	36.5	41.0	30.0	41.0
20-21	36.6068295374328	39.5	35.5	41.0	30.0	41.0
22-23	36.492908994466916	39.0	35.0	41.0	29.0	41.0
24-25	36.366196733955675	39.0	35.0	41.0	28.0	41.0
26-27	36.21057689254644	39.0	35.0	41.0	27.0	41.0
28-29	36.080117267758006	39.0	35.0	41.0	26.5	41.0
30-31	36.00024936305323	39.0	35.0	41.0	25.0	41.0
32-33	35.90156459336961	39.0	35.0	41.0	25.0	41.0
34-35	35.77897691459394	39.0	35.0	41.0	25.0	41.0
36-37	35.66509621015351	39.0	35.0	40.5	25.0	41.0
38-39	35.48457192966042	39.0	35.0	40.0	25.0	41.0
40-41	35.26558750462243	38.0	34.0	40.0	25.0	41.0
42-43	34.996404498624024	38.0	33.5	40.0	25.0	41.0
44-45	34.780374997130664	38.0	33.0	40.0	25.0	41.0
46-47	34.621486426471435	37.5	33.0	40.0	25.0	41.0
48-49	34.53145582617702	37.0	33.0	40.0	25.0	41.0
50-51	34.273754768882256	36.5	33.0	39.5	25.0	41.0
52-53	33.93958209272255	36.0	33.0	39.0	25.0	41.0
54-55	33.77403879305625	35.0	32.5	39.0	25.0	41.0
56-57	33.65096006264568	35.0	32.5	39.0	25.0	41.0
58-59	33.37803995554396	35.0	32.0	37.5	25.0	40.0
60-61	33.10379901461618	35.0	32.0	37.0	25.0	40.0
62-63	32.84851479732041	35.0	32.0	36.5	25.0	40.0
64-65	32.6829218312233	35.0	32.0	36.0	25.0	39.0
66-67	32.53514186340843	35.0	32.0	36.0	25.0	39.0
68-69	32.36226456382175	35.0	32.0	35.0	24.0	39.0
70-71	32.166982068356674	35.0	31.0	35.0	24.0	37.5
72-73	31.968592099179645	35.0	31.0	35.0	23.5	37.0
74-75	31.76480878630317	35.0	31.0	35.0	23.0	36.5
76-77	31.532430232066396	35.0	31.0	35.0	23.0	36.0
78-79	31.332003824681713	35.0	31.0	35.0	20.5	36.0
80-81	31.0996876283899	35.0	30.0	35.0	20.0	36.0
82-83	30.891635201715122	35.0	30.0	35.0	20.0	36.0
84-85	30.687629397890284	35.0	30.0	35.0	18.5	36.0
86-87	30.412839491703483	35.0	29.5	35.0	16.0	36.0
88-89	30.113578788438737	35.0	29.0	35.0	13.5	36.0
90-91	29.677129964816096	34.0	28.5	35.0	10.0	35.5
92-93	29.123940722473378	34.0	27.0	35.0	9.0	35.0
94-95	28.398034405182866	34.0	25.0	35.0	8.0	35.0
96-97	27.470752955461904	33.5	22.5	35.0	7.0	35.0
98-99	26.2815962690064	33.0	18.5	35.0	7.0	35.0
100	24.570126090880315	30.0	10.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.510955649459639
1101	2	0.4835172786280566
1101	3	0.4560979770845357
1101	4	0.4231819491241069
1101	5	0.34426105153801956
1101	6	0.3514073330810419
1101	7	0.3454294388262724
1101	8	0.35933903137460277
1101	9	0.4023280167427714
1101	10-11	0.432379898548497
1101	12-13	0.4610464381094914
1101	14-15	0.629171437316856
1101	16-17	0.633917080293898
1101	18-19	0.6194556464886887
1101	20-21	0.6473331729718552
1101	22-23	0.6193255803321662
1101	24-25	0.6429395333274499
1101	26-27	0.6415288070907224
1101	28-29	0.6061486168032175
1101	30-31	0.6167077961320828
1101	32-33	0.6381547191413688
1101	34-35	0.6221144522318127
1101	36-37	0.5989076708890408
1101	38-39	0.6235844929153416
1101	40-41	0.6115056493629041
1101	42-43	0.6126268976397
1101	44-45	0.6243222425375734
1101	46-47	0.6485992349231395
1101	48-49	0.6133647639193995
1101	50-51	0.5771921073234907
1101	52-53	0.578308872263591
1101	54-55	0.5249941700048808
1101	56-57	0.5343783207739463
1101	58-59	0.5784720592314159
1101	60-61	0.542398551975019
1101	62-63	0.5613263491349372
1101	64-65	0.5118397181753309
1101	66-67	0.49379933212286886
1101	68-69	0.5262615794239167
1101	70-71	0.5307424075196394
1101	72-73	0.4910458480283637
1101	74-75	0.4843705770640092
1101	76-77	0.47489970543058746
1101	78-79	0.5174339305138602
1101	80-81	0.47722269221196
1101	82-83	0.478964825677739
1101	84-85	0.4160216928488438
1101	86-87	0.43324952613695444
1101	88-89	0.36528542157080324
1101	90-91	0.3204556096103275
1101	92-93	0.230996980012808
1101	94-95	0.2636797874295347
1101	96-97	0.26866280692639677
1101	98-99	0.25796707854303236
1101	100	0.2631371544154639
1102	1	0.40057534279153373
1102	2	0.42709067323086103
1102	3	0.3761128001813887
1102	4	0.292942660190306
1102	5	0.31475290371606235
1102	6	0.3006060163498745
1102	7	0.3170900830409309
1102	8	0.31310638150211645
1102	9	0.2378013240503023
1102	10-11	0.4112864005730401
1102	12-13	0.40405770753555714
1102	14-15	0.5643506628526325
1102	16-17	0.626711886522294
1102	18-19	0.6211122298301888
1102	20-21	0.5933581372470229
1102	22-23	0.5791320753105325
1102	24-25	0.5873620858610948
1102	26-27	0.6297035235316812
1102	28-29	0.6191476082797607
1102	30-31	0.6034118994003208
1102	32-33	0.6074863783707727
1102	34-35	0.6335564964578424
1102	36-37	0.6005698497177718
1102	38-39	0.5974482054435981
1102	40-41	0.610885831719223
1102	42-43	0.589928612078225
1102	44-45	0.6146342579415318
1102	46-47	0.6110130430009519
1102	48-49	0.601155081190548
1102	50-51	0.5724164154621505
1102	52-53	0.5524646346579303
1102	54-55	0.5360741538015859
1102	56-57	0.5216309138838255
1102	58-59	0.5484685444383146
1102	60-61	0.474781628291403
1102	62-63	0.4901345628000584
1102	64-65	0.45021841922933703
1102	66-67	0.4260370449032891
1102	68-69	0.4257916133105155
1102	70-71	0.44416940148622786
1102	72-73	0.44120661004885875
1102	74-75	0.5008279858004236
1102	76-77	0.47581884059248125
1102	78-79	0.5058173552331127
1102	80-81	0.45976235056865633
1102	82-83	0.4650178185477465
1102	84-85	0.40007647705004246
1102	86-87	0.4112819093578324
1102	88-89	0.40029183340041286
1102	90-91	0.3799093606301511
1102	92-93	0.3246273194538638
1102	94-95	0.29254312031648055
1102	96-97	0.33466944938513876
1102	98-99	0.26807879429404835
1102	100	0.16700793951610748
1103	1	0.35141990361378816
1103	2	0.3332233576002466
1103	3	0.33713725519289994
1103	4	0.2746509400855004
1103	5	0.2863648288771188
1103	6	0.239672552804862
1103	7	0.2565852663219701
1103	8	0.23656797305026345
1103	9	0.31462928377240473
1103	10-11	0.3901041219694221
1103	12-13	0.44367716564109116
1103	14-15	0.5336965691804636
1103	16-17	0.5680495141663826
1103	18-19	0.5739114953989173
1103	20-21	0.5412649798798981
1103	22-23	0.5378122701655457
1103	24-25	0.5189134024291988
1103	26-27	0.5562013014303702
1103	28-29	0.5602876328019875
1103	30-31	0.5518786547845522
1103	32-33	0.5699992779589991
1103	34-35	0.5983787369629994
1103	36-37	0.5444323992523792
1103	38-39	0.5664071674080589
1103	40-41	0.5487952644823011
1103	42-43	0.6006623049349002
1103	44-45	0.6018105368793272
1103	46-47	0.6299406079482495
1103	48-49	0.5891165440884905
1103	50-51	0.553428709874801
1103	52-53	0.49975097410214886
1103	54-55	0.5330459906873628
1103	56-57	0.5240004416488517
1103	58-59	0.5273874614399858
1103	60-61	0.4854922241475492
1103	62-63	0.4746310854825282
1103	64-65	0.46177071323199215
1103	66-67	0.4615212063948846
1103	68-69	0.48846180073494594
1103	70-71	0.4444497819230264
1103	72-73	0.44041947716231533
1103	74-75	0.46407918632154477
1103	76-77	0.4540037575183504
1103	78-79	0.4819855386769021
1103	80-81	0.460938929486705
1103	82-83	0.41933991683275096
1103	84-85	0.4161444376204102
1103	86-87	0.42545844972316615
1103	88-89	0.35527627713070586
1103	90-91	0.38607886964804905
1103	92-93	0.3067737855030579
1103	94-95	0.26898921352926664
1103	96-97	0.28340167580220665
1103	98-99	0.27631740278530614
1103	100	0.2577271353784987
1104	1	0.23534140493820388
1104	2	0.23527406105355908
1104	3	0.20216705425323056
1104	4	0.1965991810887715
1104	5	0.17385123647932943
1104	6	0.1303498072680327
1104	7	0.11593698565961574
1104	8	0.144653002239572
1104	9	0.16054839547593502
1104	10-11	0.2676844800998808
1104	12-13	0.3002767086196485
1104	14-15	0.35881419526819514
1104	16-17	0.4313202765743469
1104	18-19	0.37649919532715614
1104	20-21	0.35658380802960465
1104	22-23	0.38398581731115655
1104	24-25	0.36909133089125845
1104	26-27	0.3923255463422848
1104	28-29	0.3717943953542573
1104	30-31	0.37417133501728017
1104	32-33	0.39626179681303597
1104	34-35	0.43361638070259545
1104	36-37	0.3826410599690391
1104	38-39	0.37647169055099994
1104	40-41	0.39954224417131456
1104	42-43	0.4254969414181744
1104	44-45	0.4173694340582159
1104	46-47	0.4045185009228973
1104	48-49	0.38888923769238914
1104	50-51	0.37072189110399023
1104	52-53	0.3697928613191408
1104	54-55	0.3784848931980136
1104	56-57	0.3377897472485287
1104	58-59	0.319877781583358
1104	60-61	0.2990629494043233
1104	62-63	0.3135401497713701
1104	64-65	0.27318601941926346
1104	66-67	0.2756897620245482
1104	68-69	0.3073069775034938
1104	70-71	0.34629973001700876
1104	72-73	0.3197553102570012
1104	74-75	0.35058788378750805
1104	76-77	0.33110346586313355
1104	78-79	0.35435093469624945
1104	80-81	0.3296210938717863
1104	82-83	0.310859290793875
1104	84-85	0.2680326750473725
1104	86-87	0.27269773733375047
1104	88-89	0.2549558414404416
1104	90-91	0.20554638345366172
1104	92-93	0.1841951012171421
1104	94-95	0.19741318035026367
1104	96-97	0.13835186105818664
1104	98-99	0.10871424870384772
1104	100	0.027339309416678503
1105	1	0.1903114997124007
1105	2	0.2278942221288922
1105	3	0.18992758423795308
1105	4	0.12184497687211149
1105	5	0.1442532969788246
1105	6	0.10239270884487439
1105	7	0.1171087837223439
1105	8	0.12673800590922468
1105	9	0.13006130441635833
1105	10-11	0.1823995737210211
1105	12-13	0.2387221672401978
1105	14-15	0.31320559888382604
1105	16-17	0.2976508820631878
1105	18-19	0.29204327589529555
1105	20-21	0.301425129491804
1105	22-23	0.33058925659850047
1105	24-25	0.3619390273174403
1105	26-27	0.31988996378756696
1105	28-29	0.3301676477389819
1105	30-31	0.3409692436749552
1105	32-33	0.3222845420902303
1105	34-35	0.3482585076845339
1105	36-37	0.3243222625822426
1105	38-39	0.35103580186876115
1105	40-41	0.3555363714324926
1105	42-43	0.3481161306229268
1105	44-45	0.3733878643588895
1105	46-47	0.35487213618865354
1105	48-49	0.3143963878896656
1105	50-51	0.2958248576377045
1105	52-53	0.24099865778418916
1105	54-55	0.263026293901639
1105	56-57	0.2747176955531785
1105	58-59	0.2562630979642506
1105	60-61	0.22331076227927582
1105	62-63	0.19468746963870132
1105	64-65	0.20452958878815508
1105	66-67	0.17919199504697048
1105	68-69	0.17800187153593328
1105	70-71	0.18722531141185073
1105	72-73	0.20104459551689047
1105	74-75	0.25355105953928003
1105	76-77	0.20921109441383123
1105	78-79	0.2906882421681303
1105	80-81	0.27884208597176396
1105	82-83	0.2556357926466255
1105	84-85	0.2624835866600499
1105	86-87	0.26185259119770876
1105	88-89	0.2581426331837946
1105	90-91	0.18420312432031238
1105	92-93	0.14927693061157044
1105	94-95	0.13442352974283978
1105	96-97	0.12611378513670246
1105	98-99	0.1377750207517785
1105	100	0.1375896474985403
1106	1	0.1573640225515085
1106	2	0.14677794608766703
1106	3	0.1256189971376962
1106	4	0.1518441180851724
1106	5	0.11053590132338798
1106	6	0.07753954540772412
1106	7	0.06985240814496052
1106	8	0.10051495548496092
1106	9	0.049050668882337334
1106	10-11	0.18327984426709776
1106	12-13	0.19327150545751692
1106	14-15	0.21880868364397088
1106	16-17	0.20732177127678852
1106	18-19	0.26114131162360366
1106	20-21	0.2328619063951507
1106	22-23	0.20235603046464234
1106	24-25	0.2526080711502985
1106	26-27	0.2750452161598602
1106	28-29	0.25429230689746873
1106	30-31	0.25443119432580374
1106	32-33	0.24139195393108537
1106	34-35	0.2751931581546643
1106	36-37	0.23838665195231812
1106	38-39	0.2623782224036262
1106	40-41	0.23968672596436136
1106	42-43	0.25922708236029735
1106	44-45	0.25456332750867716
1106	46-47	0.26812308250641337
1106	48-49	0.2539892540884452
1106	50-51	0.24964828561292052
1106	52-53	0.21778894152004113
1106	54-55	0.21960945671568055
1106	56-57	0.20018614607302965
1106	58-59	0.2243679692098226
1106	60-61	0.20588729420079943
1106	62-63	0.21927144604708104
1106	64-65	0.21817808275565653
1106	66-67	0.17986352049222631
1106	68-69	0.19586685331771747
1106	70-71	0.181367333098855
1106	72-73	0.15655278376292614
1106	74-75	0.1614417493541147
1106	76-77	0.1373428911698369
1106	78-79	0.16400750074311432
1106	80-81	0.1791665013768018
1106	82-83	0.18913187470391435
1106	84-85	0.17687341097593645
1106	86-87	0.21738963193001837
1106	88-89	0.2001713916860126
1106	90-91	0.16650425085036247
1106	92-93	0.16736953352917538
1106	94-95	0.15952220703738007
1106	96-97	0.13856115577330286
1106	98-99	0.15449367205467723
1106	100	0.12273186511572476
1107	1	0.17790040610886138
1107	2	0.14613311094459291
1107	3	0.17749742163718452
1107	4	0.15119076936056075
1107	5	0.16996246263128256
1107	6	0.11577752445374045
1107	7	0.12235696994851253
1107	8	0.12259105573549789
1107	9	0.1365286116687301
1107	10-11	0.15204882031370204
1107	12-13	0.22582229188931535
1107	14-15	0.2432581936312772
1107	16-17	0.24501959561500541
1107	18-19	0.2212280479693618
1107	20-21	0.19888361319019054
1107	22-23	0.20455176781964468
1107	24-25	0.2074018886835134
1107	26-27	0.22493408931845948
1107	28-29	0.2406923267029839
1107	30-31	0.20032557284224595
1107	32-33	0.1567165872079883
1107	34-35	0.22851081619928948
1107	36-37	0.2349302150907917
1107	38-39	0.22501436447048917
1107	40-41	0.1779668071885041
1107	42-43	0.17752694061145746
1107	44-45	0.21490495064450954
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2216	82-83	-0.5853576445878126
2216	84-85	-0.6084614854540362
2216	86-87	-0.5586008120466133
2216	88-89	-0.4855096465212583
2216	90-91	-0.37495971692400687
2216	92-93	-0.4017322546321047
2216	94-95	-0.3258393911549362
2216	96-97	-0.2880838293343224
2216	98-99	-0.18009604166901383
2216	100	-0.19013076351383518
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	7.0
3	30.0
4	31.0
5	610.0
6	4630.0
7	11774.0
8	22314.0
9	38237.0
10	59698.0
11	76366.0
12	85294.0
13	97258.0
14	105943.0
15	115378.0
16	121338.0
17	128970.0
18	137796.0
19	146882.0
20	158097.0
21	170053.0
22	183441.0
23	207321.0
24	269955.0
25	293358.0
26	284457.0
27	403545.0
28	377208.0
29	526237.0
30	622539.0
31	815395.0
32	1037311.0
33	1449195.0
34	1772221.0
35	3068774.0
36	3384354.0
37	3414436.0
38	1809348.0
39	423020.0
40	2785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.53673653332219	26.36517817445001	27.311132590498417	29.786952701729387
2	14.84916743919599	26.243443847980892	29.86413640232548	29.043252310497635
3	13.203746629014844	27.55113686945413	27.069656251722463	32.175460249808566
4	12.01843534182769	25.958579586144076	27.393000013287228	34.629985058741006
5	11.535033824894587	26.024507212735127	28.394538441812063	34.045920520558234
6	11.615911583518182	23.433500723674072	29.30413339336727	35.64645429944047
7	15.88058495450077	27.34103584883391	24.69908030194511	32.07929889472022
8	13.694882516698353	26.54524276510116	21.78521982481203	37.974654893388454
9	10.948650937068166	25.17778270574997	23.21117205913078	40.66239429805109
10-11	15.96748697362616	39.72478284489691	22.187732540182644	22.119997641294283
12-13	16.87839880429304	40.66792866637195	20.398366317395713	22.055306211939296
14-15	19.09838841558135	38.1135262562334	20.258723515468414	22.529361812716836
16-17	18.248884112177066	37.10664778127455	22.239478162408002	22.404989944140375
18-19	18.2193671372138	34.968320388489424	24.905899087514566	21.906413386782216
20-21	16.904961131863736	35.031497169873305	25.921778591693183	22.14176310656978
22-23	18.172956906937337	36.7640598953786	25.490363274531543	19.57261992315252
24-25	19.164523781409986	37.63019456944513	23.705513796002272	19.49976785314261
26-27	21.38646998109404	38.283802818369615	22.444480627732545	17.885246572803794
28-29	21.137301761588443	39.60989070217589	23.090581526833702	16.162226009401966
30-31	21.75327579369934	39.76340759342447	22.648981839173306	15.834334773702885
32-33	22.455859969663216	39.720100252611026	21.997348658710994	15.826691119014761
34-35	23.100979734329048	39.45073309957252	21.643255530213978	15.805031635884461
36-37	24.184328187177982	39.40441311057493	20.77255496638698	15.638703735860105
38-39	24.93964341594072	40.00427243235476	20.337905226966324	14.718178924738199
40-41	25.290476480676983	39.88505757660041	20.339935859515936	14.484530083206677
42-43	25.620226185435474	38.75798930008483	21.195847779339875	14.425936735139825
44-45	25.561509472209092	37.30533324144873	22.315097049752765	14.818060236589414
46-47	25.64452085477429	36.43898466107253	23.426248008297843	14.490246475855336
48-49	25.51935637421348	37.0059717617931	24.129648348573344	13.345023515420074
50-51	26.246202825064245	37.70443392368069	23.85890935522918	12.190453896025884
52-53	26.754752381580328	38.722274576283766	23.018701434874973	11.504271607260932
54-55	26.308214884216973	40.119026596560175	22.382165859584248	11.190592659638606
56-57	25.563046930312545	40.82440745476493	21.985794122291807	11.626751492630715
58-59	25.014539716617744	39.9982867362479	22.900725529854537	12.086448017279816
60-61	24.77548261032091	38.51929547768728	24.380707097468548	12.324514814523257
62-63	24.570695878464612	36.99873107567827	25.391245556273308	13.039327489583814
64-65	23.583620969862885	36.48451367888085	25.825477871704205	14.106387479552057
66-67	22.29730827287993	35.563915760567305	26.653600552887003	15.485175413665761
68-69	22.276844465869225	34.42326074365168	26.366940199201583	16.932954591277507
70-71	22.755238562741038	33.556306924244964	26.42998972993686	17.258464783077134
72-73	23.61882493647655	33.66347120995846	26.22889978989719	16.488804063667793
74-75	23.3869447721389	34.396623738196865	25.659538836867917	16.55689265279631
76-77	21.70393303620078	35.68269165466548	25.338320594651265	17.275054714482465
78-79	20.060192126937803	37.26536885699001	24.68427121862638	17.990167797445803
80-81	18.374049714081295	39.829861206905164	24.3340196286636	17.46206945034994
82-83	16.481728755153252	42.523058255041136	24.927064280999613	16.068148708806003
84-85	14.795955228006774	45.27660787431561	25.19997988177219	14.72745701590543
86-87	13.373689255882933	48.086675854756805	24.885981404520287	13.653653484839973
88-89	12.669206588092097	51.07101063957763	23.69899066880193	12.560792103528339
90-91	12.414926351172548	53.99778640040168	21.876081454397674	11.711205794028093
92-93	12.4476825372995	56.030341871352604	20.216649025297755	11.305326566050141
94-95	12.792345563325897	56.65509135145827	19.12618628764853	11.42637679756731
96-97	13.365001499818993	56.22709470728531	18.727220446445394	11.680683346450305
98-99	14.280583068735181	54.704930719487386	18.66453727926843	12.349948932508998
100	15.638758919116889	53.3679517478314	18.202869120937322	12.790420212114395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	778064.0
1	666386.5
2	352270.5
3	107982.5
4	64567.0
5	59731.5
6	50992.0
7	41021.0
8	34175.5
9	32451.0
10	35250.5
11	41799.0
12	52981.0
13	70174.0
14	93661.0
15	123410.5
16	157890.0
17	194605.5
18	232555.5
19	269723.5
20	303111.0
21	331401.5
22	354821.5
23	374167.0
24	388467.0
25	395852.5
26	396716.5
27	390326.5
28	376165.0
29	357132.5
30	335200.0
31	310506.0
32	285722.5
33	262531.5
34	241125.0
35	222748.0
36	207995.0
37	199000.5
38	197054.0
39	203628.5
40	222451.0
41	257924.0
42	319495.0
43	418202.5
44	559256.5
45	738871.5
46	934142.0
47	1098388.5
48	1181325.5
49	1158466.0
50	1048692.5
51	898535.0
52	756480.0
53	644969.0
54	556944.0
55	474898.5
56	384823.5
57	290384.5
58	203504.5
59	143653.0
60	94916.0
61	50863.5
62	29040.5
63	18958.5
64	14031.5
65	11328.0
66	9686.0
67	8426.0
68	7423.0
69	6726.0
70	6084.5
71	5508.0
72	4977.0
73	4601.0
74	4344.5
75	3951.0
76	3655.0
77	3399.0
78	3151.0
79	2952.0
80	2814.5
81	2715.5
82	2594.0
83	2443.0
84	2334.5
85	2267.5
86	2116.0
87	2046.5
88	1941.0
89	1786.0
90	1672.0
91	1580.0
92	1464.0
93	1322.5
94	1230.5
95	1140.5
96	1045.0
97	918.0
98	794.0
99	675.5
100	622.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.42034995865178E-4
2	5.223222428590417E-4
3	6.414483684233844E-5
4	6.277030462428834E-4
5	5.223222428590417E-4
6	7.972286864690636E-4
7	8.384646530105669E-4
8	0.001145443515041758
9	3.986143432345318E-4
10-11	3.573783766930285E-4
12-13	8.819915065821538E-4
14-15	0.0024397946870389443
16-17	0.0012485334313955163
18-19	0.003507348043057863
20-21	0.0036150197334717886
22-23	0.003747891181216632
24-25	0.004856680503777053
26-27	0.007035314069386478
28-29	0.00906962175210064
30-31	0.009351400856800913
32-33	0.008870314580483376
34-35	0.011207019351168562
36-37	0.010503717032932921
38-39	0.009039840220709555
40-41	0.007500364136493432
42-43	0.004343521809038347
44-45	0.005181986462048913
46-47	0.007438510186681176
48-49	0.0060044149058488955
50-51	0.0037982906958784696
52-53	0.004206068587233335
54-55	0.004146505524451164
56-57	0.006742080529535788
58-59	0.008079958555104561
60-61	0.00772028929138145
62-63	0.009184166103604816
64-65	0.010091357367517888
66-67	0.00906962175210064
68-69	0.01139487208763541
70-71	0.011314691041582486
72-73	0.013548305895913916
74-75	0.018134661730141114
76-77	0.018991453479392347
78-79	0.01900748968860293
80-81	0.023225012710986686
82-83	0.02144270260158171
84-85	0.021073869789738266
86-87	0.021099069547069183
88-89	0.021635137112108727
90-91	0.02101888850101626
92-93	0.0217084454970714
94-95	0.01911057960495669
96-97	0.021261722526205114
98-99	0.01830189648333721
100	0.01869363816548149
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.1825607E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.16798883186422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.97087756210617	84.61559261079985
2	3.6471631563000138	6.431260808652925
3	0.25618065703493237	0.6776079992518665
4	0.04463192114378862	0.15740426899800797
5	0.021092463681870732	0.09298400511698401
6	0.011212859219726036	0.05931691478748425
7	0.009022878607410875	0.0556870342202628
8	0.006304667797564568	0.04446959039714295
9	0.003346818605680135	0.02655740389030934
>10	0.024740298965849006	0.41883348678756077
>50	0.0027928011519941904	0.17346209993889974
>100	0.0019469504748958395	0.33992299071855697
>500	2.9801082257380164E-4	0.17704527796638408
>1k	2.2824858828181935E-4	0.45042519686824034
>5k	3.628833852121494E-5	0.21707945698663653
>10k+	1.2441716064413096E-4	6.062350854618952
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	570804	2.6152949606395826	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124663	0.5711776996626028	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107362	0.4919084266476529	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	98863	0.45296792891029336	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46552	0.21329074604889567	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38645	0.17706265855515496	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34091	0.1561972594851543	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32973	0.15107483608588754	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30645	0.14040846607381868	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27285	0.12501370523165747	No Hit
CCAGCTCACCACCACCATTCCGCCACCATCCCCCGCCGCCAGATCGGAAG	25325	0.11603342807373009	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23851	0.10927989310904387	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002524557507152035	0.0	0.0	0.0	0.0
2	0.006616081742881194	0.0	0.0	1.3745322180501097E-5	0.0
3	0.011513998213199752	0.0	0.0	2.7490644361002194E-5	0.0
4	0.01942214024104805	0.0	0.0	4.123596654150329E-5	0.0
5	0.03211365438771073	0.0	0.0	4.581774060167032E-5	0.0
6	0.0534922121524501	0.0	0.0	5.0399514661837354E-5	0.0
7	0.07897603947509914	0.0	0.0	5.0399514661837354E-5	0.0
8	0.16724849851827717	0.0	0.0	5.0399514661837354E-5	0.0
9	0.49474912656495645	0.0	0.0	5.498128872200439E-5	0.0
10-11	1.2042482942169719	0.0	0.0	5.727217575208791E-5	0.0
12-13	2.1797629729152552	0.0	0.0	5.956306278217142E-5	0.0
14-15	3.3612925404548886	0.0	0.0	6.87266109025055E-5	0.0
16-17	4.669476088339719	0.0	0.0	7.330838496267252E-5	0.0
18-19	6.110996134036501	0.0	0.0	9.163548120334064E-5	0.0
20-21	7.99295753836308	0.0	0.0	1.0538080338384174E-4	0.0
22-23	10.200256973379938	0.0	0.0	1.0538080338384174E-4	0.0
24-25	12.645586443483566	0.0	0.0	1.0538080338384174E-4	0.0
26-27	15.549001684122691	0.0	0.0	1.0767169041392526E-4	0.0
28-29	19.42839665352721	0.0	0.0	1.0996257744400878E-4	0.0
30-31	24.36318953236902	0.0	0.0	1.1683523853425932E-4	0.0
32-33	29.52394176253609	0.0	0.0	1.3287144774484392E-4	0.0
34-35	33.66386785943685	0.0	0.0	1.5119854398551206E-4	0.0
36-37	36.451437524738715	0.0	0.0	1.5119854398551206E-4	0.0
38-39	38.27749441287017	0.0	0.0	1.695256402261802E-4	0.0
40-41	39.70503317502235	0.0	0.0	1.878527364668483E-4	0.0
42-43	40.79804057683252	0.0	0.0	2.3825225112868569E-4	0.0
44-45	41.562848630051846	0.0	0.0	2.4741579924901974E-4	0.0
46-47	42.17693922556197	0.0	0.0	2.74906443610022E-4	0.0
48-49	42.71758856466168	0.0	0.0	3.0239708797102417E-4	0.0
50-51	43.26098467731046	0.0	0.0	3.527966026328615E-4	0.0
52-53	43.84905766881994	0.0	0.0	3.802872469938637E-4	0.0
54-55	44.46898544448271	0.0	0.0	4.146505524451164E-4	2.2908870300835163E-6
56-57	45.12948482944827	0.0	0.0	4.3297764868578453E-4	4.581774060167033E-6
58-59	45.808993994989464	0.0	0.0	4.558865189866197E-4	4.581774060167033E-6
60-61	46.4820107866874	0.0	0.0	5.085769206785405E-4	4.581774060167033E-6
62-63	47.09053452671442	0.0	0.0	5.498128872200439E-4	4.581774060167033E-6
64-65	47.63796489142318	0.0	0.0	5.795944186111296E-4	4.581774060167033E-6
66-67	48.116886279497294	0.0	0.0	6.43739255453468E-4	4.581774060167033E-6
68-69	48.52492075019953	0.0	0.0	6.643572387242196E-4	4.581774060167033E-6
70-71	48.88865862928807	0.0	0.0	6.849752219949713E-4	4.581774060167033E-6
72-73	49.226486576066364	0.0	0.0	7.1017497932589E-4	4.581774060167033E-6
74-75	49.54602408079647	0.0	0.0	7.491200588373097E-4	4.581774060167033E-6
76-77	49.8576511526117	0.0	0.0	8.063922345893977E-4	4.581774060167033E-6
78-79	50.15644238439737	0.0	0.0	9.003186028228218E-4	4.581774060167033E-6
80-81	50.44606319540162	2.2908870300835163E-6	0.0	0.0010515171468083338	6.8726610902505485E-6
82-83	50.730062169634046	6.8726610902505485E-6	0.0	0.0011110802095905053	9.163548120334065E-6
84-85	51.01247585004165	9.163548120334065E-6	0.0	0.0011248255317710064	9.163548120334065E-6
86-87	51.29615639097689	9.163548120334065E-6	0.0	0.0011339890798913405	9.163548120334065E-6
88	51.511492899143654	9.163548120334065E-6	0.0	0.0011500252891019252	9.163548120334065E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCTCA	4745	0.0	78.53792	2
AGCTCAC	4840	0.0	77.2873	3
CCAGCTC	5085	0.0	73.655945	1
GCTCACC	7280	0.0	52.02883	4
TTGCTGA	7435	0.0	44.940407	9
GCTTGCT	1535	0.0	41.33013	7
TACCCGA	16100	0.0	40.717937	6
CTACCCG	16020	0.0	39.102547	5
ACCCGAC	17435	0.0	38.08567	7
CCCGACG	18600	0.0	37.16594	8
CTTGCTG	4950	0.0	36.361263	8
CATTCCG	4935	0.0	35.473454	16-17
TGACGCC	1375	0.0	34.1773	3
CTGCTGA	7960	0.0	33.356754	9
ATTCCGC	5105	0.0	33.279507	16-17
CCTGCTG	5660	0.0	32.29822	8
GTTGCTG	2215	0.0	32.036762	8
AGTTGCC	640	0.0	31.57395	3
TCACCAC	12385	0.0	31.113958	6
TTGCGCC	1395	0.0	30.992315	3
>>END_MODULE