FastQCFastQC Report
Thu 26 May 2016
SRR1248492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21825607
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT556240.254856600322731No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438100.20072752157591767No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420500.1926635992300237No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417270.19118368620858975No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387250.17742920047996832No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384170.17601801406943687No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353370.16190614996412242No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349320.16005053146975476No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330240.15130850656295608No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330120.15125352527423408No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327110.1498744112821238No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326780.1497232127381383No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314460.1440784670960125No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311780.14285055164788774No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282630.12949468026250083No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281050.12877075996099446No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279160.1279048046636229No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262390.12022116956472277No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261190.11967135667750271No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261040.11960263006660021No Hit
GGCGGCGGGGGATGGTGGCGGAATGGTGGTGGTGAGCTGGAGATCGGAAG258550.11846176832561862No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253280.11604717339591059No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246840.11309651090116303No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232680.1066087188319665No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231500.1060680694928668No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230250.10549534773534593No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224640.10292497248759222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTA141400.061.856276
CGGCGGG56500.061.5563743
TGCTCTA119950.058.1068956
GCCCTAC346550.057.1634527
CCCCTAC921650.057.1440967
CCCCCTA515000.054.3269656
GCCCCTA100950.054.145226
GCGGCGG66700.052.428052
GGCGGCG68300.051.8485641
CTCCCTA352300.051.7347036
GCCTTTT22100.051.071831
CCGCCTA58250.050.9120036
CGCCCTA145800.050.415726
TCCCCTA301700.050.3636446
CCCGGAA103400.048.7714846
TTGCCCT65000.048.7344745
TTGCTCT48550.048.693225
ATGCCCT47400.048.2881285
GTTACAC23750.048.285087
CCGGAAG343450.047.5256427
Produced by FastQC (version 0.11.5)